Restriction map for
pF1KB5390
(Linear) (Six-base) MAPSORT of: KIBB5390.seq Check: 8014 from: 1 to: 2760
KIBB5390.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AatII G_ACGT'C
Cuts at: 0 1871 2760
Size: 1871 889
AccI GT'mk_AC
Cuts at: 0 1646 2760
Size: 1646 1114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1491 2093 2760
Size: 1491 602 667
Fragments arranged by size:
1491 667 602
AflIII A'CryG_T
Cuts at: 0 752 818 1008 2760
Size: 752 66 190 1752
Fragments arranged by size:
1752 752 190 66
AleI CACnn'nnGTG
Cuts at: 0 1267 2239 2401 2760
Size: 1267 972 162 359
Fragments arranged by size:
1267 972 359 162
AlwNI CAG_nnn'CTG
Cuts at: 0 28 352 1080 2760
Size: 28 324 728 1680
Fragments arranged by size:
1680 728 324 28
ApaI G_GGCC'C
Cuts at: 0 1880 1928 2760
Size: 1880 48 832
Fragments arranged by size:
1880 832 48
AvaI C'yCGr_G
Cuts at: 0 554 1736 2381 2760
Size: 554 1182 645 379
Fragments arranged by size:
1182 645 554 379
BamHI G'GATC_C
Cuts at: 0 1380 2760
Size: 1380 1380
BanI G'GyrC_C
Cuts at: 0 116 299 1949 2701 2760
Size: 116 183 1650 752 59
Fragments arranged by size:
1650 752 183 116 59
BanII G_rGCy'C
Cuts at: 0 578 908 1621 1880 1928 2231 2760
Size: 578 330 713 259 48 303 529
Fragments arranged by size:
713 578 529 330 303 259 48
BbeI G_GCGC'C
Cuts at: 0 120 303 2705 2760
Size: 120 183 2402 55
Fragments arranged by size:
2402 183 120 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 590 1356 1497 2760
Size: 590 766 141 1263
Fragments arranged by size:
1263 766 590 141
BbvCI CC'TCA_GC
Cuts at: 0 850 2153 2760
Size: 850 1303 607
Fragments arranged by size:
1303 850 607
BclI T'GATC_A
Cuts at: 0 683 2760
Size: 683 2077
BglI GCCn_nnn'nGGC
Cuts at: 0 1998 2760
Size: 1998 762
BglII A'GATC_T
Cuts at: 0 1433 2760
Size: 1433 1327
Bme1580I G_kGCm'C
Cuts at: 0 1276 1736 1880 1928 1954 2760
Size: 1276 460 144 48 26 806
Fragments arranged by size:
1276 806 460 144 48 26
BmgBI CAC'GTC
Cuts at: 0 2664 2760
Size: 2664 96
BmrI ACTGGGnnnn_n'
Cuts at: 0 451 683 934 1895 2118 2760
Size: 451 232 251 961 223 642
Fragments arranged by size:
961 642 451 251 232 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 919 1955 2272 2760
Size: 225 694 1036 317 488
Fragments arranged by size:
1036 694 488 317 225
Bpu10I CC'TnA_GC
Cuts at: 0 850 1907 2153 2760
Size: 850 1057 246 607
Fragments arranged by size:
1057 850 607 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 688 1605 2760
Size: 289 399 917 1155
Fragments arranged by size:
1155 917 399 289
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 1895 2239 2760
Size: 688 1207 344 521
Fragments arranged by size:
1207 688 521 344
BsaAI yAC'GTr
Cuts at: 0 219 821 1797 2163 2187 2760
Size: 219 602 976 366 24 573
Fragments arranged by size:
976 602 573 366 219 24
BsaBI GATnn'nnATC
Cuts at: 0 2341 2760
Size: 2341 419
BsaHI Gr'CG_yC
Cuts at: 0 117 300 1868 2673 2702 2760
Size: 117 183 1568 805 29 58
Fragments arranged by size:
1568 805 183 117 58 29
BsaWI w'CCGG_w
Cuts at: 0 1170 2469 2760
Size: 1170 1299 291
Fragments arranged by size:
1299 1170 291
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 979 1009 1069 1099 1669 1699
Size: 223 30 726 30 60 30 570 30
Cuts at: 1699 1703 1733 1747 1777 2581 2611 2760
Size: 4 30 14 30 804 30 149
Fragments arranged by size:
804 726 570 223 149 60 30 30
30 30 30 30 30 14 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 916 1555 1867 2760
Size: 103 813 639 312 893
Fragments arranged by size:
893 813 639 312 103
BseYI C'CCAG_C
Cuts at: 0 345 721 1162 1921 1927 2087 2706 2734
Size: 345 376 441 759 6 160 619 28
Cuts at: 2734 2760
Size: 26
Fragments arranged by size:
759 619 441 376 345 160 28 26
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 2760
Size: 241 2519
BsiEI CG_ry'CG
Cuts at: 0 11 1171 2058 2690 2760
Size: 11 1160 887 632 70
Fragments arranged by size:
1160 887 632 70 11
BsiHKAI G_wGCw'C
Cuts at: 0 908 2231 2760
Size: 908 1323 529
Fragments arranged by size:
1323 908 529
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2601 2760
Size: 2601 159
Bsp1286I G_dGCh'C
Cuts at: 0 578 908 1276 1621 1736 1880 1928 1954
Size: 578 330 368 345 115 144 48 26
Cuts at: 1954 2231 2760
Size: 277 529
Fragments arranged by size:
578 529 368 345 330 277 144 115
48 26
BspEI T'CCGG_A
Cuts at: 0 2469 2760
Size: 2469 291
BspHI T'CATG_A
Cuts at: 0 740 827 2760
Size: 740 87 1933
Fragments arranged by size:
1933 740 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1658 2760
Size: 1658 1102
BsrBI CCG'CTC
Cuts at: 0 561 995 2760
Size: 561 434 1765
Fragments arranged by size:
1765 561 434
BsrDI GCAATG_nn'
Cuts at: 0 861 1293 2096 2760
Size: 861 432 803 664
Fragments arranged by size:
861 803 664 432
BsrFI r'CCGG_y
Cuts at: 0 163 1406 1781 2689 2760
Size: 163 1243 375 908 71
Fragments arranged by size:
1243 908 375 163 71
BsrGI T'GTAC_A
Cuts at: 0 2477 2760
Size: 2477 283
BssSI C'ACGA_G
Cuts at: 0 85 1411 2401 2760
Size: 85 1326 990 359
Fragments arranged by size:
1326 990 359 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 821 2760
Size: 821 1939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2313 2760
Size: 2313 447
BstYI r'GATC_y
Cuts at: 0 1380 1433 1440 2760
Size: 1380 53 7 1320
Fragments arranged by size:
1380 1320 53 7
BtgI C'CryG_G
Cuts at: 0 1245 1376 2760
Size: 1245 131 1384
Fragments arranged by size:
1384 1245 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1169 2478 2760
Size: 1169 1309 282
Fragments arranged by size:
1309 1169 282
BtsI GCAGTG_nn'
Cuts at: 0 154 211 850 1330 2760
Size: 154 57 639 480 1430
Fragments arranged by size:
1430 639 480 154 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 2593 2760
Size: 2593 167
EaeI y'GGCC_r
Cuts at: 0 393 984 1029 2565 2687 2760
Size: 393 591 45 1536 122 73
Fragments arranged by size:
1536 591 393 122 73 45
EagI C'GGCC_G
Cuts at: 0 2687 2760
Size: 2687 73
EarI CTCTTCn'nnn_
Cuts at: 0 755 1258 2236 2650 2760
Size: 755 503 978 414 110
Fragments arranged by size:
978 755 503 414 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1230 2760
Size: 1230 1530
EcoICRI GAG'CTC
Cuts at: 0 906 2229 2760
Size: 906 1323 531
Fragments arranged by size:
1323 906 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 919 1491 1955 2093 2272 2760
Size: 225 694 572 464 138 179 488
Fragments arranged by size:
694 572 488 464 225 179 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 2254 2760
Size: 307 1947 506
Fragments arranged by size:
1947 506 307
EcoO109I rG'GnC_Cy
Cuts at: 0 1113 1758 1877 2421 2760
Size: 1113 645 119 544 339
Fragments arranged by size:
1113 645 544 339 119
EcoRV GAT'ATC
Cuts at: 0 1575 2760
Size: 1575 1185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1371 2760
Size: 1371 1389
FspI TGC'GCA
Cuts at: 0 547 2760
Size: 547 2213
HaeII r_GCGC'y
Cuts at: 0 120 303 2705 2760
Size: 120 183 2402 55
Fragments arranged by size:
2402 183 120 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 1069 1101 1425 1457 1616 1648
Size: 223 32 814 32 324 32 159 32
Cuts at: 1648 1703 1735 2760
Size: 55 32 1025
Fragments arranged by size:
1025 814 324 223 159 55 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 116 299 2701 2760
Size: 116 183 2402 59
Fragments arranged by size:
2402 183 116 59
MfeI C'AATT_G
Cuts at: 0 1968 2760
Size: 1968 792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 2528 2760
Size: 737 1791 232
Fragments arranged by size:
1791 737 232
MscI TGG'CCA
Cuts at: 0 395 986 1031 2760
Size: 395 591 45 1729
Fragments arranged by size:
1729 591 395 45
MslI CAynn'nnrTG
Cuts at: 0 1267 1986 2239 2401 2760
Size: 1267 719 253 162 359
Fragments arranged by size:
1267 719 359 253 162
MspA1I CmG'CkG
Cuts at: 0 25 55 352 374 732 2016 2298 2564
Size: 25 30 297 22 358 1284 282 266
Cuts at: 2564 2760
Size: 196
Fragments arranged by size:
1284 358 297 282 266 196 30 25
22
NaeI GCC'GGC
Cuts at: 0 165 2691 2760
Size: 165 2526 69
Fragments arranged by size:
2526 165 69
NarI GG'CG_CC
Cuts at: 0 117 300 2702 2760
Size: 117 183 2402 58
Fragments arranged by size:
2402 183 117 58
NcoI C'CATG_G
Cuts at: 0 1376 2760
Size: 1376 1384
NgoMIV G'CCGG_C
Cuts at: 0 163 2689 2760
Size: 163 2526 71
Fragments arranged by size:
2526 163 71
NspI r_CATG'y
Cuts at: 0 739 756 1012 2523 2760
Size: 739 17 256 1511 237
Fragments arranged by size:
1511 739 256 237 17
PasI CC'CwG_GG
Cuts at: 0 1873 2760
Size: 1873 887
PciI A'CATG_T
Cuts at: 0 752 1008 2760
Size: 752 256 1752
Fragments arranged by size:
1752 752 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 2664 2760
Size: 1050 1614 96
Fragments arranged by size:
1614 1050 96
PfoI T'CCnGG_A
Cuts at: 0 1094 1961 2417 2760
Size: 1094 867 456 343
Fragments arranged by size:
1094 867 456 343
PmlI CAC'GTG
Cuts at: 0 821 2760
Size: 821 1939
PpuMI rG'GwC_Cy
Cuts at: 0 1113 2421 2760
Size: 1113 1308 339
Fragments arranged by size:
1308 1113 339
PspOMI G'GGCC_C
Cuts at: 0 1876 1924 2760
Size: 1876 48 836
Fragments arranged by size:
1876 836 48
PstI C_TGCA'G
Cuts at: 0 845 2030 2216 2725 2760
Size: 845 1185 186 509 35
Fragments arranged by size:
1185 845 509 186 35
PvuII CAG'CTG
Cuts at: 0 25 352 374 732 2016 2298 2760
Size: 25 327 22 358 1284 282 462
Fragments arranged by size:
1284 462 358 327 282 25 22
SacI G_AGCT'C
Cuts at: 0 908 2231 2760
Size: 908 1323 529
Fragments arranged by size:
1323 908 529
SapI GCTCTTCn'nnn_
Cuts at: 0 2236 2760
Size: 2236 524
ScaI AGT'ACT
Cuts at: 0 877 2760
Size: 877 1883
SexAI A'CCwGG_T
Cuts at: 0 365 962 2760
Size: 365 597 1798
Fragments arranged by size:
1798 597 365
SfcI C'TryA_G
Cuts at: 0 841 2026 2212 2721 2760
Size: 841 1185 186 509 39
Fragments arranged by size:
1185 841 509 186 39
SfoI GGC'GCC
Cuts at: 0 118 301 2703 2760
Size: 118 183 2402 57
Fragments arranged by size:
2402 183 118 57
SmaI CCC'GGG
Cuts at: 0 1738 2760
Size: 1738 1022
SmlI C'TyrA_G
Cuts at: 0 304 667 1620 2760
Size: 304 363 953 1140
Fragments arranged by size:
1140 953 363 304
SphI G_CATG'C
Cuts at: 0 2523 2760
Size: 2523 237
StuI AGG'CCT
Cuts at: 0 101 952 2760
Size: 101 851 1808
Fragments arranged by size:
1808 851 101
StyI C'CwwG_G
Cuts at: 0 1376 1974 1996 2627 2760
Size: 1376 598 22 631 133
Fragments arranged by size:
1376 631 598 133 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 2748 2760
Size: 417 2331 12
Fragments arranged by size:
2331 417 12
TatI w'GTAC_w
Cuts at: 0 875 2477 2760
Size: 875 1602 283
Fragments arranged by size:
1602 875 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 979 1011 2760
Size: 97 32 850 32 1749
Fragments arranged by size:
1749 850 97 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2176 2760
Size: 2176 584
XbaI T'CTAG_A
Cuts at: 0 384 2760
Size: 384 2376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 353 2760
Size: 353 2407
XmaI C'CCGG_G
Cuts at: 0 1736 2760
Size: 1736 1024
ZraI GAC'GTC
Cuts at: 0 1869 2760
Size: 1869 891
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BfrBI BlpI BmtI BplI BsiWI BsmI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII EcoRI FseI FspAI HincII HindIII HpaI KpnI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XhoI XmnI