Restriction map for pF1KB5390
 (Linear) (Six-base) MAPSORT of: KIBB5390.seq  Check: 8014  from: 1  to: 2760



KIBB5390.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AatII G_ACGT'C

Cuts at:      0    1871    2760 
   Size:       1871     889
 
AccI GT'mk_AC

Cuts at:      0    1646    2760 
   Size:       1646    1114
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1491    2093    2760 
   Size:       1491     602     667

  Fragments arranged by size: 

                1491     667     602
 
AflIII A'CryG_T

Cuts at:      0     752     818    1008    2760 
   Size:        752      66     190    1752

  Fragments arranged by size: 

                1752     752     190      66
 
AleI CACnn'nnGTG

Cuts at:      0    1267    2239    2401    2760 
   Size:       1267     972     162     359

  Fragments arranged by size: 

                1267     972     359     162
 
AlwNI CAG_nnn'CTG

Cuts at:      0      28     352    1080    2760 
   Size:         28     324     728    1680

  Fragments arranged by size: 

                1680     728     324      28
 
ApaI G_GGCC'C

Cuts at:      0    1880    1928    2760 
   Size:       1880      48     832

  Fragments arranged by size: 

                1880     832      48
 
AvaI C'yCGr_G

Cuts at:      0     554    1736    2381    2760 
   Size:        554    1182     645     379

  Fragments arranged by size: 

                1182     645     554     379
 
BamHI G'GATC_C

Cuts at:      0    1380    2760 
   Size:       1380    1380
 
BanI G'GyrC_C

Cuts at:      0     116     299    1949    2701    2760 
   Size:        116     183    1650     752      59

  Fragments arranged by size: 

                1650     752     183     116      59
 
BanII G_rGCy'C

Cuts at:      0     578     908    1621    1880    1928    2231    2760 
   Size:        578     330     713     259      48     303     529

  Fragments arranged by size: 

                 713     578     529     330     303     259      48
 
BbeI G_GCGC'C

Cuts at:      0     120     303    2705    2760 
   Size:        120     183    2402      55

  Fragments arranged by size: 

                2402     183     120      55
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     590    1356    1497    2760 
   Size:        590     766     141    1263

  Fragments arranged by size: 

                1263     766     590     141
 
BbvCI CC'TCA_GC

Cuts at:      0     850    2153    2760 
   Size:        850    1303     607

  Fragments arranged by size: 

                1303     850     607
 
BclI T'GATC_A

Cuts at:      0     683    2760 
   Size:        683    2077
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1998    2760 
   Size:       1998     762
 
BglII A'GATC_T

Cuts at:      0    1433    2760 
   Size:       1433    1327
 
Bme1580I G_kGCm'C

Cuts at:      0    1276    1736    1880    1928    1954    2760 
   Size:       1276     460     144      48      26     806

  Fragments arranged by size: 

                1276     806     460     144      48      26
 
BmgBI CAC'GTC

Cuts at:      0    2664    2760 
   Size:       2664      96
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     451     683     934    1895    2118    2760 
   Size:        451     232     251     961     223     642

  Fragments arranged by size: 

                 961     642     451     251     232     223
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     919    1955    2272    2760 
   Size:        225     694    1036     317     488

  Fragments arranged by size: 

                1036     694     488     317     225
 
Bpu10I CC'TnA_GC

Cuts at:      0     850    1907    2153    2760 
   Size:        850    1057     246     607

  Fragments arranged by size: 

                1057     850     607     246
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     688    1605    2760 
   Size:        289     399     917    1155

  Fragments arranged by size: 

                1155     917     399     289
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     688    1895    2239    2760 
   Size:        688    1207     344     521

  Fragments arranged by size: 

                1207     688     521     344
 
BsaAI yAC'GTr

Cuts at:      0     219     821    1797    2163    2187    2760 
   Size:        219     602     976     366      24     573

  Fragments arranged by size: 

                 976     602     573     366     219      24
 
BsaBI GATnn'nnATC

Cuts at:      0    2341    2760 
   Size:       2341     419
 
BsaHI Gr'CG_yC

Cuts at:      0     117     300    1868    2673    2702    2760 
   Size:        117     183    1568     805      29      58

  Fragments arranged by size: 

                1568     805     183     117      58      29
 
BsaWI w'CCGG_w

Cuts at:      0    1170    2469    2760 
   Size:       1170    1299     291

  Fragments arranged by size: 

                1299    1170     291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     223     253     979    1009    1069    1099    1669    1699 
   Size:        223      30     726      30      60      30     570      30

Cuts at:   1699    1703    1733    1747    1777    2581    2611    2760 
   Size:          4      30      14      30     804      30     149

  Fragments arranged by size: 

                 804     726     570     223     149      60      30      30
                  30      30      30      30      30      14       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     916    1555    1867    2760 
   Size:        103     813     639     312     893

  Fragments arranged by size: 

                 893     813     639     312     103
 
BseYI C'CCAG_C

Cuts at:      0     345     721    1162    1921    1927    2087    2706    2734 
   Size:        345     376     441     759       6     160     619      28

Cuts at:   2734    2760 
   Size:         26

  Fragments arranged by size: 

                 759     619     441     376     345     160      28      26
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241    2760 
   Size:        241    2519
 
BsiEI CG_ry'CG

Cuts at:      0      11    1171    2058    2690    2760 
   Size:         11    1160     887     632      70

  Fragments arranged by size: 

                1160     887     632      70      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     908    2231    2760 
   Size:        908    1323     529

  Fragments arranged by size: 

                1323     908     529
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2601    2760 
   Size:       2601     159
 
Bsp1286I G_dGCh'C

Cuts at:      0     578     908    1276    1621    1736    1880    1928    1954 
   Size:        578     330     368     345     115     144      48      26

Cuts at:   1954    2231    2760 
   Size:        277     529

  Fragments arranged by size: 

                 578     529     368     345     330     277     144     115
                  48      26
 
BspEI T'CCGG_A

Cuts at:      0    2469    2760 
   Size:       2469     291
 
BspHI T'CATG_A

Cuts at:      0     740     827    2760 
   Size:        740      87    1933

  Fragments arranged by size: 

                1933     740      87
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1658    2760 
   Size:       1658    1102
 
BsrBI CCG'CTC

Cuts at:      0     561     995    2760 
   Size:        561     434    1765

  Fragments arranged by size: 

                1765     561     434
 
BsrDI GCAATG_nn'

Cuts at:      0     861    1293    2096    2760 
   Size:        861     432     803     664

  Fragments arranged by size: 

                 861     803     664     432
 
BsrFI r'CCGG_y

Cuts at:      0     163    1406    1781    2689    2760 
   Size:        163    1243     375     908      71

  Fragments arranged by size: 

                1243     908     375     163      71
 
BsrGI T'GTAC_A

Cuts at:      0    2477    2760 
   Size:       2477     283
 
BssSI C'ACGA_G

Cuts at:      0      85    1411    2401    2760 
   Size:         85    1326     990     359

  Fragments arranged by size: 

                1326     990     359      85
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     821    2760 
   Size:        821    1939
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2313    2760 
   Size:       2313     447
 
BstYI r'GATC_y

Cuts at:      0    1380    1433    1440    2760 
   Size:       1380      53       7    1320

  Fragments arranged by size: 

                1380    1320      53       7
 
BtgI C'CryG_G

Cuts at:      0    1245    1376    2760 
   Size:       1245     131    1384

  Fragments arranged by size: 

                1384    1245     131
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1169    2478    2760 
   Size:       1169    1309     282

  Fragments arranged by size: 

                1309    1169     282
 
BtsI GCAGTG_nn'

Cuts at:      0     154     211     850    1330    2760 
   Size:        154      57     639     480    1430

  Fragments arranged by size: 

                1430     639     480     154      57
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2593    2760 
   Size:       2593     167
 
EaeI y'GGCC_r

Cuts at:      0     393     984    1029    2565    2687    2760 
   Size:        393     591      45    1536     122      73

  Fragments arranged by size: 

                1536     591     393     122      73      45
 
EagI C'GGCC_G

Cuts at:      0    2687    2760 
   Size:       2687      73
 
EarI CTCTTCn'nnn_

Cuts at:      0     755    1258    2236    2650    2760 
   Size:        755     503     978     414     110

  Fragments arranged by size: 

                 978     755     503     414     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1230    2760 
   Size:       1230    1530
 
EcoICRI GAG'CTC

Cuts at:      0     906    2229    2760 
   Size:        906    1323     531

  Fragments arranged by size: 

                1323     906     531
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     919    1491    1955    2093    2272    2760 
   Size:        225     694     572     464     138     179     488

  Fragments arranged by size: 

                 694     572     488     464     225     179     138
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     307    2254    2760 
   Size:        307    1947     506

  Fragments arranged by size: 

                1947     506     307
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1113    1758    1877    2421    2760 
   Size:       1113     645     119     544     339

  Fragments arranged by size: 

                1113     645     544     339     119
 
EcoRV GAT'ATC

Cuts at:      0    1575    2760 
   Size:       1575    1185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1371    2760 
   Size:       1371    1389
 
FspI TGC'GCA

Cuts at:      0     547    2760 
   Size:        547    2213
 
HaeII r_GCGC'y

Cuts at:      0     120     303    2705    2760 
   Size:        120     183    2402      55

  Fragments arranged by size: 

                2402     183     120      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255    1069    1101    1425    1457    1616    1648 
   Size:        223      32     814      32     324      32     159      32

Cuts at:   1648    1703    1735    2760 
   Size:         55      32    1025

  Fragments arranged by size: 

                1025     814     324     223     159      55      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0     116     299    2701    2760 
   Size:        116     183    2402      59

  Fragments arranged by size: 

                2402     183     116      59
 
MfeI C'AATT_G

Cuts at:      0    1968    2760 
   Size:       1968     792
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    2528    2760 
   Size:        737    1791     232

  Fragments arranged by size: 

                1791     737     232
 
MscI TGG'CCA

Cuts at:      0     395     986    1031    2760 
   Size:        395     591      45    1729

  Fragments arranged by size: 

                1729     591     395      45
 
MslI CAynn'nnrTG

Cuts at:      0    1267    1986    2239    2401    2760 
   Size:       1267     719     253     162     359

  Fragments arranged by size: 

                1267     719     359     253     162
 
MspA1I CmG'CkG

Cuts at:      0      25      55     352     374     732    2016    2298    2564 
   Size:         25      30     297      22     358    1284     282     266

Cuts at:   2564    2760 
   Size:        196

  Fragments arranged by size: 

                1284     358     297     282     266     196      30      25
                  22
 
NaeI GCC'GGC

Cuts at:      0     165    2691    2760 
   Size:        165    2526      69

  Fragments arranged by size: 

                2526     165      69
 
NarI GG'CG_CC

Cuts at:      0     117     300    2702    2760 
   Size:        117     183    2402      58

  Fragments arranged by size: 

                2402     183     117      58
 
NcoI C'CATG_G

Cuts at:      0    1376    2760 
   Size:       1376    1384
 
NgoMIV G'CCGG_C

Cuts at:      0     163    2689    2760 
   Size:        163    2526      71

  Fragments arranged by size: 

                2526     163      71
 
NspI r_CATG'y

Cuts at:      0     739     756    1012    2523    2760 
   Size:        739      17     256    1511     237

  Fragments arranged by size: 

                1511     739     256     237      17
 
PasI CC'CwG_GG

Cuts at:      0    1873    2760 
   Size:       1873     887
 
PciI A'CATG_T

Cuts at:      0     752    1008    2760 
   Size:        752     256    1752

  Fragments arranged by size: 

                1752     752     256
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1050    2664    2760 
   Size:       1050    1614      96

  Fragments arranged by size: 

                1614    1050      96
 
PfoI T'CCnGG_A

Cuts at:      0    1094    1961    2417    2760 
   Size:       1094     867     456     343

  Fragments arranged by size: 

                1094     867     456     343
 
PmlI CAC'GTG

Cuts at:      0     821    2760 
   Size:        821    1939
 
PpuMI rG'GwC_Cy

Cuts at:      0    1113    2421    2760 
   Size:       1113    1308     339

  Fragments arranged by size: 

                1308    1113     339
 
PspOMI G'GGCC_C

Cuts at:      0    1876    1924    2760 
   Size:       1876      48     836

  Fragments arranged by size: 

                1876     836      48
 
PstI C_TGCA'G

Cuts at:      0     845    2030    2216    2725    2760 
   Size:        845    1185     186     509      35

  Fragments arranged by size: 

                1185     845     509     186      35
 
PvuII CAG'CTG

Cuts at:      0      25     352     374     732    2016    2298    2760 
   Size:         25     327      22     358    1284     282     462

  Fragments arranged by size: 

                1284     462     358     327     282      25      22
 
SacI G_AGCT'C

Cuts at:      0     908    2231    2760 
   Size:        908    1323     529

  Fragments arranged by size: 

                1323     908     529
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2236    2760 
   Size:       2236     524
 
ScaI AGT'ACT

Cuts at:      0     877    2760 
   Size:        877    1883
 
SexAI A'CCwGG_T

Cuts at:      0     365     962    2760 
   Size:        365     597    1798

  Fragments arranged by size: 

                1798     597     365
 
SfcI C'TryA_G

Cuts at:      0     841    2026    2212    2721    2760 
   Size:        841    1185     186     509      39

  Fragments arranged by size: 

                1185     841     509     186      39
 
SfoI GGC'GCC

Cuts at:      0     118     301    2703    2760 
   Size:        118     183    2402      57

  Fragments arranged by size: 

                2402     183     118      57
 
SmaI CCC'GGG

Cuts at:      0    1738    2760 
   Size:       1738    1022
 
SmlI C'TyrA_G

Cuts at:      0     304     667    1620    2760 
   Size:        304     363     953    1140

  Fragments arranged by size: 

                1140     953     363     304
 
SphI G_CATG'C

Cuts at:      0    2523    2760 
   Size:       2523     237
 
StuI AGG'CCT

Cuts at:      0     101     952    2760 
   Size:        101     851    1808

  Fragments arranged by size: 

                1808     851     101
 
StyI C'CwwG_G

Cuts at:      0    1376    1974    1996    2627    2760 
   Size:       1376     598      22     631     133

  Fragments arranged by size: 

                1376     631     598     133      22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     417    2748    2760 
   Size:        417    2331      12

  Fragments arranged by size: 

                2331     417      12
 
TatI w'GTAC_w

Cuts at:      0     875    2477    2760 
   Size:        875    1602     283

  Fragments arranged by size: 

                1602     875     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      97     129     979    1011    2760 
   Size:         97      32     850      32    1749

  Fragments arranged by size: 

                1749     850      97      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2176    2760 
   Size:       2176     584
 
XbaI T'CTAG_A

Cuts at:      0     384    2760 
   Size:        384    2376
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     353    2760 
   Size:        353    2407
 
XmaI C'CCGG_G

Cuts at:      0    1736    2760 
   Size:       1736    1024
 
ZraI GAC'GTC

Cuts at:      0    1869    2760 
   Size:       1869     891
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AleI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BclI     BglI
    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI     SapI     ScaI    SexAI
     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BcgI    BciVI    BfrBI     BlpI     BmtI     BplI    BsiWI     BsmI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII    EcoRI     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     XhoI     XmnI
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