(Linear) (Six-base) MAPSORT of: KIBB5390.seq Check: 8014 from: 1 to: 2760 KIBB5390.orf With 182 enzymes: SgfI * February 28, 2007 11:27 .. AatII G_ACGT'C Cuts at: 0 1871 2760 Size: 1871 889 AccI GT'mk_AC Cuts at: 0 1646 2760 Size: 1646 1114 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1491 2093 2760 Size: 1491 602 667 Fragments arranged by size: 1491 667 602 AflIII A'CryG_T Cuts at: 0 752 818 1008 2760 Size: 752 66 190 1752 Fragments arranged by size: 1752 752 190 66 AleI CACnn'nnGTG Cuts at: 0 1267 2239 2401 2760 Size: 1267 972 162 359 Fragments arranged by size: 1267 972 359 162 AlwNI CAG_nnn'CTG Cuts at: 0 28 352 1080 2760 Size: 28 324 728 1680 Fragments arranged by size: 1680 728 324 28 ApaI G_GGCC'C Cuts at: 0 1880 1928 2760 Size: 1880 48 832 Fragments arranged by size: 1880 832 48 AvaI C'yCGr_G Cuts at: 0 554 1736 2381 2760 Size: 554 1182 645 379 Fragments arranged by size: 1182 645 554 379 BamHI G'GATC_C Cuts at: 0 1380 2760 Size: 1380 1380 BanI G'GyrC_C Cuts at: 0 116 299 1949 2701 2760 Size: 116 183 1650 752 59 Fragments arranged by size: 1650 752 183 116 59 BanII G_rGCy'C Cuts at: 0 578 908 1621 1880 1928 2231 2760 Size: 578 330 713 259 48 303 529 Fragments arranged by size: 713 578 529 330 303 259 48 BbeI G_GCGC'C Cuts at: 0 120 303 2705 2760 Size: 120 183 2402 55 Fragments arranged by size: 2402 183 120 55 BbsI GAAGACnn'nnnn_ Cuts at: 0 590 1356 1497 2760 Size: 590 766 141 1263 Fragments arranged by size: 1263 766 590 141 BbvCI CC'TCA_GC Cuts at: 0 850 2153 2760 Size: 850 1303 607 Fragments arranged by size: 1303 850 607 BclI T'GATC_A Cuts at: 0 683 2760 Size: 683 2077 BglI GCCn_nnn'nGGC Cuts at: 0 1998 2760 Size: 1998 762 BglII A'GATC_T Cuts at: 0 1433 2760 Size: 1433 1327 Bme1580I G_kGCm'C Cuts at: 0 1276 1736 1880 1928 1954 2760 Size: 1276 460 144 48 26 806 Fragments arranged by size: 1276 806 460 144 48 26 BmgBI CAC'GTC Cuts at: 0 2664 2760 Size: 2664 96 BmrI ACTGGGnnnn_n' Cuts at: 0 451 683 934 1895 2118 2760 Size: 451 232 251 961 223 642 Fragments arranged by size: 961 642 451 251 232 223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 919 1955 2272 2760 Size: 225 694 1036 317 488 Fragments arranged by size: 1036 694 488 317 225 Bpu10I CC'TnA_GC Cuts at: 0 850 1907 2153 2760 Size: 850 1057 246 607 Fragments arranged by size: 1057 850 607 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 688 1605 2760 Size: 289 399 917 1155 Fragments arranged by size: 1155 917 399 289 BsaI GGTCTCn'nnnn_ Cuts at: 0 688 1895 2239 2760 Size: 688 1207 344 521 Fragments arranged by size: 1207 688 521 344 BsaAI yAC'GTr Cuts at: 0 219 821 1797 2163 2187 2760 Size: 219 602 976 366 24 573 Fragments arranged by size: 976 602 573 366 219 24 BsaBI GATnn'nnATC Cuts at: 0 2341 2760 Size: 2341 419 BsaHI Gr'CG_yC Cuts at: 0 117 300 1868 2673 2702 2760 Size: 117 183 1568 805 29 58 Fragments arranged by size: 1568 805 183 117 58 29 BsaWI w'CCGG_w Cuts at: 0 1170 2469 2760 Size: 1170 1299 291 Fragments arranged by size: 1299 1170 291 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 979 1009 1069 1099 1669 1699 Size: 223 30 726 30 60 30 570 30 Cuts at: 1699 1703 1733 1747 1777 2581 2611 2760 Size: 4 30 14 30 804 30 149 Fragments arranged by size: 804 726 570 223 149 60 30 30 30 30 30 30 30 14 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 916 1555 1867 2760 Size: 103 813 639 312 893 Fragments arranged by size: 893 813 639 312 103 BseYI C'CCAG_C Cuts at: 0 345 721 1162 1921 1927 2087 2706 2734 Size: 345 376 441 759 6 160 619 28 Cuts at: 2734 2760 Size: 26 Fragments arranged by size: 759 619 441 376 345 160 28 26 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 2760 Size: 241 2519 BsiEI CG_ry'CG Cuts at: 0 11 1171 2058 2690 2760 Size: 11 1160 887 632 70 Fragments arranged by size: 1160 887 632 70 11 BsiHKAI G_wGCw'C Cuts at: 0 908 2231 2760 Size: 908 1323 529 Fragments arranged by size: 1323 908 529 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2601 2760 Size: 2601 159 Bsp1286I G_dGCh'C Cuts at: 0 578 908 1276 1621 1736 1880 1928 1954 Size: 578 330 368 345 115 144 48 26 Cuts at: 1954 2231 2760 Size: 277 529 Fragments arranged by size: 578 529 368 345 330 277 144 115 48 26 BspEI T'CCGG_A Cuts at: 0 2469 2760 Size: 2469 291 BspHI T'CATG_A Cuts at: 0 740 827 2760 Size: 740 87 1933 Fragments arranged by size: 1933 740 87 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1658 2760 Size: 1658 1102 BsrBI CCG'CTC Cuts at: 0 561 995 2760 Size: 561 434 1765 Fragments arranged by size: 1765 561 434 BsrDI GCAATG_nn' Cuts at: 0 861 1293 2096 2760 Size: 861 432 803 664 Fragments arranged by size: 861 803 664 432 BsrFI r'CCGG_y Cuts at: 0 163 1406 1781 2689 2760 Size: 163 1243 375 908 71 Fragments arranged by size: 1243 908 375 163 71 BsrGI T'GTAC_A Cuts at: 0 2477 2760 Size: 2477 283 BssSI C'ACGA_G Cuts at: 0 85 1411 2401 2760 Size: 85 1326 990 359 Fragments arranged by size: 1326 990 359 85 BstAPI GCAn_nnn'nTGC Cuts at: 0 821 2760 Size: 821 1939 BstXI CCAn_nnnn'nTGG Cuts at: 0 2313 2760 Size: 2313 447 BstYI r'GATC_y Cuts at: 0 1380 1433 1440 2760 Size: 1380 53 7 1320 Fragments arranged by size: 1380 1320 53 7 BtgI C'CryG_G Cuts at: 0 1245 1376 2760 Size: 1245 131 1384 Fragments arranged by size: 1384 1245 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1169 2478 2760 Size: 1169 1309 282 Fragments arranged by size: 1309 1169 282 BtsI GCAGTG_nn' Cuts at: 0 154 211 850 1330 2760 Size: 154 57 639 480 1430 Fragments arranged by size: 1430 639 480 154 57 DrdI GACnn_nn'nnGTC Cuts at: 0 2593 2760 Size: 2593 167 EaeI y'GGCC_r Cuts at: 0 393 984 1029 2565 2687 2760 Size: 393 591 45 1536 122 73 Fragments arranged by size: 1536 591 393 122 73 45 EagI C'GGCC_G Cuts at: 0 2687 2760 Size: 2687 73 EarI CTCTTCn'nnn_ Cuts at: 0 755 1258 2236 2650 2760 Size: 755 503 978 414 110 Fragments arranged by size: 978 755 503 414 110 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1230 2760 Size: 1230 1530 EcoICRI GAG'CTC Cuts at: 0 906 2229 2760 Size: 906 1323 531 Fragments arranged by size: 1323 906 531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 225 919 1491 1955 2093 2272 2760 Size: 225 694 572 464 138 179 488 Fragments arranged by size: 694 572 488 464 225 179 138 EcoNI CCTnn'n_nnAGG Cuts at: 0 307 2254 2760 Size: 307 1947 506 Fragments arranged by size: 1947 506 307 EcoO109I rG'GnC_Cy Cuts at: 0 1113 1758 1877 2421 2760 Size: 1113 645 119 544 339 Fragments arranged by size: 1113 645 544 339 119 EcoRV GAT'ATC Cuts at: 0 1575 2760 Size: 1575 1185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1371 2760 Size: 1371 1389 FspI TGC'GCA Cuts at: 0 547 2760 Size: 547 2213 HaeII r_GCGC'y Cuts at: 0 120 303 2705 2760 Size: 120 183 2402 55 Fragments arranged by size: 2402 183 120 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 223 255 1069 1101 1425 1457 1616 1648 Size: 223 32 814 32 324 32 159 32 Cuts at: 1648 1703 1735 2760 Size: 55 32 1025 Fragments arranged by size: 1025 814 324 223 159 55 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 116 299 2701 2760 Size: 116 183 2402 59 Fragments arranged by size: 2402 183 116 59 MfeI C'AATT_G Cuts at: 0 1968 2760 Size: 1968 792 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 737 2528 2760 Size: 737 1791 232 Fragments arranged by size: 1791 737 232 MscI TGG'CCA Cuts at: 0 395 986 1031 2760 Size: 395 591 45 1729 Fragments arranged by size: 1729 591 395 45 MslI CAynn'nnrTG Cuts at: 0 1267 1986 2239 2401 2760 Size: 1267 719 253 162 359 Fragments arranged by size: 1267 719 359 253 162 MspA1I CmG'CkG Cuts at: 0 25 55 352 374 732 2016 2298 2564 Size: 25 30 297 22 358 1284 282 266 Cuts at: 2564 2760 Size: 196 Fragments arranged by size: 1284 358 297 282 266 196 30 25 22 NaeI GCC'GGC Cuts at: 0 165 2691 2760 Size: 165 2526 69 Fragments arranged by size: 2526 165 69 NarI GG'CG_CC Cuts at: 0 117 300 2702 2760 Size: 117 183 2402 58 Fragments arranged by size: 2402 183 117 58 NcoI C'CATG_G Cuts at: 0 1376 2760 Size: 1376 1384 NgoMIV G'CCGG_C Cuts at: 0 163 2689 2760 Size: 163 2526 71 Fragments arranged by size: 2526 163 71 NspI r_CATG'y Cuts at: 0 739 756 1012 2523 2760 Size: 739 17 256 1511 237 Fragments arranged by size: 1511 739 256 237 17 PasI CC'CwG_GG Cuts at: 0 1873 2760 Size: 1873 887 PciI A'CATG_T Cuts at: 0 752 1008 2760 Size: 752 256 1752 Fragments arranged by size: 1752 752 256 PflMI CCAn_nnn'nTGG Cuts at: 0 1050 2664 2760 Size: 1050 1614 96 Fragments arranged by size: 1614 1050 96 PfoI T'CCnGG_A Cuts at: 0 1094 1961 2417 2760 Size: 1094 867 456 343 Fragments arranged by size: 1094 867 456 343 PmlI CAC'GTG Cuts at: 0 821 2760 Size: 821 1939 PpuMI rG'GwC_Cy Cuts at: 0 1113 2421 2760 Size: 1113 1308 339 Fragments arranged by size: 1308 1113 339 PspOMI G'GGCC_C Cuts at: 0 1876 1924 2760 Size: 1876 48 836 Fragments arranged by size: 1876 836 48 PstI C_TGCA'G Cuts at: 0 845 2030 2216 2725 2760 Size: 845 1185 186 509 35 Fragments arranged by size: 1185 845 509 186 35 PvuII CAG'CTG Cuts at: 0 25 352 374 732 2016 2298 2760 Size: 25 327 22 358 1284 282 462 Fragments arranged by size: 1284 462 358 327 282 25 22 SacI G_AGCT'C Cuts at: 0 908 2231 2760 Size: 908 1323 529 Fragments arranged by size: 1323 908 529 SapI GCTCTTCn'nnn_ Cuts at: 0 2236 2760 Size: 2236 524 ScaI AGT'ACT Cuts at: 0 877 2760 Size: 877 1883 SexAI A'CCwGG_T Cuts at: 0 365 962 2760 Size: 365 597 1798 Fragments arranged by size: 1798 597 365 SfcI C'TryA_G Cuts at: 0 841 2026 2212 2721 2760 Size: 841 1185 186 509 39 Fragments arranged by size: 1185 841 509 186 39 SfoI GGC'GCC Cuts at: 0 118 301 2703 2760 Size: 118 183 2402 57 Fragments arranged by size: 2402 183 118 57 SmaI CCC'GGG Cuts at: 0 1738 2760 Size: 1738 1022 SmlI C'TyrA_G Cuts at: 0 304 667 1620 2760 Size: 304 363 953 1140 Fragments arranged by size: 1140 953 363 304 SphI G_CATG'C Cuts at: 0 2523 2760 Size: 2523 237 StuI AGG'CCT Cuts at: 0 101 952 2760 Size: 101 851 1808 Fragments arranged by size: 1808 851 101 StyI C'CwwG_G Cuts at: 0 1376 1974 1996 2627 2760 Size: 1376 598 22 631 133 Fragments arranged by size: 1376 631 598 133 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 417 2748 2760 Size: 417 2331 12 Fragments arranged by size: 2331 417 12 TatI w'GTAC_w Cuts at: 0 875 2477 2760 Size: 875 1602 283 Fragments arranged by size: 1602 875 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 979 1011 2760 Size: 97 32 850 32 1749 Fragments arranged by size: 1749 850 97 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2176 2760 Size: 2176 584 XbaI T'CTAG_A Cuts at: 0 384 2760 Size: 384 2376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 353 2760 Size: 353 2407 XmaI C'CCGG_G Cuts at: 0 1736 2760 Size: 1736 1024 ZraI GAC'GTC Cuts at: 0 1869 2760 Size: 1869 891 Enzymes that do cut: AatII AccI AcuI AflIII AleI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BlpI BmtI BplI BsiWI BsmI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRI FseI FspAI HincII HindIII HpaI KpnI MluI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI XmnI