Restriction map for
pF1KB5394
(Linear) (Six-base) MAPSORT of: KIBB5394.seq Check: 6443 from: 1 to: 1779
KIBB5394.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 1779
Size: 1685 94
AfeI AGC'GCT
Cuts at: 0 1308 1779
Size: 1308 471
AflIII A'CryG_T
Cuts at: 0 380 719 1091 1779
Size: 380 339 372 688
Fragments arranged by size:
688 380 372 339
AhdI GACnn_n'nnGTC
Cuts at: 0 408 1779
Size: 408 1371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 1779
Size: 196 1583
AlwNI CAG_nnn'CTG
Cuts at: 0 96 351 366 1501 1779
Size: 96 255 15 1135 278
Fragments arranged by size:
1135 278 255 96 15
ApoI r'AATT_y
Cuts at: 0 147 1779
Size: 147 1632
AvaI C'yCGr_G
Cuts at: 0 922 970 1140 1753 1779
Size: 922 48 170 613 26
Fragments arranged by size:
922 613 170 48 26
BanI G'GyrC_C
Cuts at: 0 172 461 554 1218 1318 1510 1779
Size: 172 289 93 664 100 192 269
Fragments arranged by size:
664 289 269 192 172 100 93
BanII G_rGCy'C
Cuts at: 0 275 626 889 1210 1265 1304 1422 1779
Size: 275 351 263 321 55 39 118 357
Fragments arranged by size:
357 351 321 275 263 118 55 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1740 1779
Size: 1740 39
BbvCI CC'TCA_GC
Cuts at: 0 1075 1779
Size: 1075 704
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1364 1398 1779
Size: 1364 34 381
Fragments arranged by size:
1364 381 34
BglI GCCn_nnn'nGGC
Cuts at: 0 279 961 1779
Size: 279 682 818
Fragments arranged by size:
818 682 279
Bme1580I G_kGCm'C
Cuts at: 0 175 459 1321 1779
Size: 175 284 862 458
Fragments arranged by size:
862 458 284 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 543 848 1122 1779
Size: 543 305 274 657
Fragments arranged by size:
657 543 305 274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 763 829 881 1436 1443 1750 1779
Size: 73 690 66 52 555 7 307 29
Fragments arranged by size:
690 555 307 73 66 52 29 7
Bpu10I CC'TnA_GC
Cuts at: 0 1075 1779
Size: 1075 704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1606 1779
Size: 553 1053 173
Fragments arranged by size:
1053 553 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 1480 1779
Size: 106 1374 299
Fragments arranged by size:
1374 299 106
BsaAI yAC'GTr
Cuts at: 0 383 1779
Size: 383 1396
BsaHI Gr'CG_yC
Cuts at: 0 1745 1779
Size: 1745 34
BsaWI w'CCGG_w
Cuts at: 0 194 1004 1779
Size: 194 810 775
Fragments arranged by size:
810 775 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 667 697 1779
Size: 193 30 444 30 1082
Fragments arranged by size:
1082 444 193 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 634 664 688 1779
Size: 631 3 30 24 1091
Fragments arranged by size:
1091 631 30 24 3
BseYI C'CCAG_C
Cuts at: 0 96 848 1303 1339 1494 1580 1779
Size: 96 752 455 36 155 86 199
Fragments arranged by size:
752 455 199 155 96 86 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1779
Size: 73 1706
BsiEI CG_ry'CG
Cuts at: 0 956 1352 1779
Size: 956 396 427
Fragments arranged by size:
956 427 396
BsiHKAI G_wGCw'C
Cuts at: 0 626 1779
Size: 626 1153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1429 1779
Size: 1429 350
Bsp1286I G_dGCh'C
Cuts at: 0 175 275 459 626 889 1210 1265 1304
Size: 175 100 184 167 263 321 55 39
Cuts at: 1304 1321 1422 1779
Size: 17 101 357
Fragments arranged by size:
357 321 263 184 175 167 101 100
55 39 17
BspEI T'CCGG_A
Cuts at: 0 194 1004 1779
Size: 194 810 775
Fragments arranged by size:
810 775 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 605 1416 1556 1779
Size: 332 273 811 140 223
Fragments arranged by size:
811 332 273 223 140
BsrDI GCAATG_nn'
Cuts at: 0 416 1779
Size: 416 1363
BsrFI r'CCGG_y
Cuts at: 0 951 955 1279 1779
Size: 951 4 324 500
Fragments arranged by size:
951 500 324 4
BssHII G'CGCG_C
Cuts at: 0 71 1779
Size: 71 1708
BssSI C'ACGA_G
Cuts at: 0 226 940 1779
Size: 226 714 839
Fragments arranged by size:
839 714 226
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1170 1779
Size: 1170 609
BstYI r'GATC_y
Cuts at: 0 189 663 1070 1779
Size: 189 474 407 709
Fragments arranged by size:
709 474 407 189
BtgI C'CryG_G
Cuts at: 0 374 1246 1514 1740 1779
Size: 374 872 268 226 39
Fragments arranged by size:
872 374 268 226 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 717 1779
Size: 717 1062
BtsI GCAGTG_nn'
Cuts at: 0 142 1533 1779
Size: 142 1391 246
Fragments arranged by size:
1391 246 142
DrdI GACnn_nn'nnGTC
Cuts at: 0 1096 1779
Size: 1096 683
EaeI y'GGCC_r
Cuts at: 0 953 1349 1595 1779
Size: 953 396 246 184
Fragments arranged by size:
953 396 246 184
EagI C'GGCC_G
Cuts at: 0 953 1349 1779
Size: 953 396 430
Fragments arranged by size:
953 430 396
EarI CTCTTCn'nnn_
Cuts at: 0 1472 1661 1779
Size: 1472 189 118
Fragments arranged by size:
1472 189 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1016 1779
Size: 1016 763
EcoICRI GAG'CTC
Cuts at: 0 624 1779
Size: 624 1155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 763 829 881 1436 1443 1685 1750
Size: 73 690 66 52 555 7 242 65
Cuts at: 1750 1779
Size: 29
Fragments arranged by size:
690 555 242 73 66 65 52 29
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 872 1084 1779
Size: 872 212 695
Fragments arranged by size:
872 695 212
EcoO109I rG'GnC_Cy
Cuts at: 0 1137 1144 1251 1360 1400 1773 1779
Size: 1137 7 107 109 40 373 6
Fragments arranged by size:
1137 373 109 107 40 7 6
HaeII r_GCGC'y
Cuts at: 0 367 1310 1542 1779
Size: 367 943 232 237
Fragments arranged by size:
943 367 237 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 667 699 1779
Size: 394 32 241 32 1080
Fragments arranged by size:
1080 394 241 32 32
HincII GTy'rAC
Cuts at: 0 717 936 1779
Size: 717 219 843
Fragments arranged by size:
843 717 219
MslI CAynn'nnrTG
Cuts at: 0 1693 1779
Size: 1693 86
MspA1I CmG'CkG
Cuts at: 0 162 1779
Size: 162 1617
NaeI GCC'GGC
Cuts at: 0 953 957 1281 1779
Size: 953 4 324 498
Fragments arranged by size:
953 498 324 4
NcoI C'CATG_G
Cuts at: 0 374 1246 1779
Size: 374 872 533
Fragments arranged by size:
872 533 374
NgoMIV G'CCGG_C
Cuts at: 0 951 955 1279 1779
Size: 951 4 324 500
Fragments arranged by size:
951 500 324 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 351 1779
Size: 96 255 1428
Fragments arranged by size:
1428 255 96
PpuMI rG'GwC_Cy
Cuts at: 0 1251 1400 1773 1779
Size: 1251 149 373 6
Fragments arranged by size:
1251 373 149 6
PstI C_TGCA'G
Cuts at: 0 185 260 572 671 1619 1779
Size: 185 75 312 99 948 160
Fragments arranged by size:
948 312 185 160 99 75
PvuII CAG'CTG
Cuts at: 0 162 1779
Size: 162 1617
SacI G_AGCT'C
Cuts at: 0 626 1779
Size: 626 1153
SanDI GG'GwC_CC
Cuts at: 0 1400 1773 1779
Size: 1400 373 6
Fragments arranged by size:
1400 373 6
SapI GCTCTTCn'nnn_
Cuts at: 0 1661 1779
Size: 1661 118
SfcI C'TryA_G
Cuts at: 0 181 256 568 667 1615 1779
Size: 181 75 312 99 948 164
Fragments arranged by size:
948 312 181 164 99 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 961 1779
Size: 961 818
SmaI CCC'GGG
Cuts at: 0 972 1142 1779
Size: 972 170 637
Fragments arranged by size:
972 637 170
SmlI C'TyrA_G
Cuts at: 0 532 1621 1779
Size: 532 1089 158
Fragments arranged by size:
1089 532 158
StuI AGG'CCT
Cuts at: 0 1381 1779
Size: 1381 398
StyI C'CwwG_G
Cuts at: 0 116 374 558 1049 1246 1779
Size: 116 258 184 491 197 533
Fragments arranged by size:
533 491 258 197 184 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1236 1779
Size: 1236 543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 457 489 1030 1062 1257 1289
Size: 217 32 208 32 541 32 195 32
Cuts at: 1289 1779
Size: 490
Fragments arranged by size:
541 490 217 208 195 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1253 1411 1779
Size: 1253 158 368
Fragments arranged by size:
1253 368 158
XmaI C'CCGG_G
Cuts at: 0 970 1140 1779
Size: 970 170 639
Fragments arranged by size:
970 639 170
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MslI MspA1I
NaeI NcoI NgoMIV PflMI PpuMI PstI PvuII SacI
SanDI SapI SfcI SfiI SmaI SmlI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaBI BsiWI BsmI BspHI BsrBI BsrGI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI