Restriction map for
pF1KB5396
(Linear) (Six-base) MAPSORT of: KIBB5396.seq Check: 768 from: 1 to: 1899
KIBB5396.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 1899
Size: 1795 104
AflIII A'CryG_T
Cuts at: 0 267 1899
Size: 267 1632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1768 1800 1899
Size: 1768 32 99
Fragments arranged by size:
1768 99 32
ApoI r'AATT_y
Cuts at: 0 208 689 793 1087 1864 1899
Size: 208 481 104 294 777 35
Fragments arranged by size:
777 481 294 208 104 35
AvaI C'yCGr_G
Cuts at: 0 1682 1899
Size: 1682 217
BamHI G'GATC_C
Cuts at: 0 594 751 1899
Size: 594 157 1148
Fragments arranged by size:
1148 594 157
BanI G'GyrC_C
Cuts at: 0 543 1599 1899
Size: 543 1056 300
Fragments arranged by size:
1056 543 300
BanII G_rGCy'C
Cuts at: 0 228 1899
Size: 228 1671
BbsI GAAGACnn'nnnn_
Cuts at: 0 313 1151 1899
Size: 313 838 748
Fragments arranged by size:
838 748 313
BciVI GTATCCnnnnn_n'
Cuts at: 0 51 1869 1899
Size: 51 1818 30
Fragments arranged by size:
1818 51 30
BfrBI ATG'CAT
Cuts at: 0 625 1129 1899
Size: 625 504 770
Fragments arranged by size:
770 625 504
BlpI GC'TnA_GC
Cuts at: 0 1799 1899
Size: 1799 100
Bme1580I G_kGCm'C
Cuts at: 0 548 1602 1899
Size: 548 1054 297
Fragments arranged by size:
1054 548 297
BmrI ACTGGGnnnn_n'
Cuts at: 0 1788 1899
Size: 1788 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1260 1367 1899
Size: 1260 107 532
Fragments arranged by size:
1260 532 107
Bpu10I CC'TnA_GC
Cuts at: 0 1013 1899
Size: 1013 886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1899
Size: 412 1487
BsaHI Gr'CG_yC
Cuts at: 0 918 1899
Size: 918 981
BsaWI w'CCGG_w
Cuts at: 0 604 1899
Size: 604 1295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1145 1175 1899
Size: 1145 30 724
Fragments arranged by size:
1145 724 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1166 1304 1307 1520 1523 1899
Size: 1166 138 3 213 3 376
Fragments arranged by size:
1166 376 213 138 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1899
Size: 638 1261
BsiHKAI G_wGCw'C
Cuts at: 0 228 1533 1560 1899
Size: 228 1305 27 339
Fragments arranged by size:
1305 339 228 27
Bsp1286I G_dGCh'C
Cuts at: 0 228 548 1533 1560 1602 1899
Size: 228 320 985 27 42 297
Fragments arranged by size:
985 320 297 228 42 27
BsrDI GCAATG_nn'
Cuts at: 0 1707 1899
Size: 1707 192
BsrFI r'CCGG_y
Cuts at: 0 920 1899
Size: 920 979
BstAPI GCAn_nnn'nTGC
Cuts at: 0 196 1899
Size: 196 1703
BstYI r'GATC_y
Cuts at: 0 576 594 751 996 1461 1678 1899
Size: 576 18 157 245 465 217 221
Fragments arranged by size:
576 465 245 221 217 157 18
BtgI C'CryG_G
Cuts at: 0 1665 1674 1899
Size: 1665 9 225
Fragments arranged by size:
1665 225 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1779 1899
Size: 1779 120
BtsI GCAGTG_nn'
Cuts at: 0 1283 1899
Size: 1283 616
EarI CTCTTCn'nnn_
Cuts at: 0 35 1497 1899
Size: 35 1462 402
Fragments arranged by size:
1462 402 35
EcoICRI GAG'CTC
Cuts at: 0 226 1899
Size: 226 1673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1260 1367 1795 1899
Size: 1260 107 428 104
Fragments arranged by size:
1260 428 107 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1568 1899
Size: 1568 331
EcoO109I rG'GnC_Cy
Cuts at: 0 361 1642 1899
Size: 361 1281 257
Fragments arranged by size:
1281 361 257
EcoRI G'AATT_C
Cuts at: 0 793 1899
Size: 793 1106
HaeII r_GCGC'y
Cuts at: 0 1257 1899
Size: 1257 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 883 915 1899
Size: 586 32 265 32 984
Fragments arranged by size:
984 586 265 32 32
MfeI C'AATT_G
Cuts at: 0 191 1899
Size: 191 1708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 1899
Size: 729 1170
MslI CAynn'nnrTG
Cuts at: 0 291 724 1899
Size: 291 433 1175
Fragments arranged by size:
1175 433 291
MspA1I CmG'CkG
Cuts at: 0 1159 1899
Size: 1159 740
NaeI GCC'GGC
Cuts at: 0 922 1899
Size: 922 977
NgoMIV G'CCGG_C
Cuts at: 0 920 1899
Size: 920 979
NsiI A_TGCA'T
Cuts at: 0 627 1131 1899
Size: 627 504 768
Fragments arranged by size:
768 627 504
NspI r_CATG'y
Cuts at: 0 271 1326 1899
Size: 271 1055 573
Fragments arranged by size:
1055 573 271
PciI A'CATG_T
Cuts at: 0 267 1899
Size: 267 1632
PpuMI rG'GwC_Cy
Cuts at: 0 361 1642 1899
Size: 361 1281 257
Fragments arranged by size:
1281 361 257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 390 422 1899
Size: 390 32 1477
Fragments arranged by size:
1477 390 32
PvuII CAG'CTG
Cuts at: 0 1159 1899
Size: 1159 740
SacI G_AGCT'C
Cuts at: 0 228 1899
Size: 228 1671
SfcI C'TryA_G
Cuts at: 0 238 312 1899
Size: 238 74 1587
Fragments arranged by size:
1587 238 74
SmlI C'TyrA_G
Cuts at: 0 391 1899
Size: 391 1508
SphI G_CATG'C
Cuts at: 0 1326 1899
Size: 1326 573
SspI AAT'ATT
Cuts at: 0 146 1067 1899
Size: 146 921 832
Fragments arranged by size:
921 832 146
StuI AGG'CCT
Cuts at: 0 110 385 1273 1899
Size: 110 275 888 626
Fragments arranged by size:
888 626 275 110
StyI C'CwwG_G
Cuts at: 0 517 708 1543 1899
Size: 517 191 835 356
Fragments arranged by size:
835 517 356 191
TatI w'GTAC_w
Cuts at: 0 234 464 1899
Size: 234 230 1435
Fragments arranged by size:
1435 234 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 1899
Size: 426 32 1441
Fragments arranged by size:
1441 426 32
XmnI GAAnn'nnTTC
Cuts at: 0 146 1899
Size: 146 1753
Enzymes that do cut:
AcuI AflIII AloI ApoI AvaI BamHI BanI BanII
BbsI BciVI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BsrDI BsrFI BstAPI BstYI BtgI BtgZI BtsI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MfeI
MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PciI
PpuMI PsrI PvuII SacI SfcI SmlI SphI SspI
StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BglI
BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI MscI NarI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI ZraI