Restriction map for
pF1KB5398
(Linear) (Six-base) MAPSORT of: KIBB5398.seq Check: 8777 from: 1 to: 543
KIBB5398.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 481 543
Size: 481 62
AflII C'TTAA_G
Cuts at: 0 109 543
Size: 109 434
AhdI GACnn_n'nnGTC
Cuts at: 0 516 543
Size: 516 27
AlwNI CAG_nnn'CTG
Cuts at: 0 455 543
Size: 455 88
BanI G'GyrC_C
Cuts at: 0 233 543
Size: 233 310
BanII G_rGCy'C
Cuts at: 0 320 347 497 543
Size: 320 27 150 46
Fragments arranged by size:
320 150 46 27
BbeI G_GCGC'C
Cuts at: 0 237 543
Size: 237 306
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 543
Size: 94 449
BglI GCCn_nnn'nGGC
Cuts at: 0 231 543
Size: 231 312
BmgBI CAC'GTC
Cuts at: 0 201 543
Size: 201 342
BsaHI Gr'CG_yC
Cuts at: 0 234 543
Size: 234 309
BsaWI w'CCGG_w
Cuts at: 0 530 543
Size: 530 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 543
Size: 328 215
BseYI C'CCAG_C
Cuts at: 0 114 426 543
Size: 114 312 117
Fragments arranged by size:
312 117 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 543
Size: 418 125
BsiEI CG_ry'CG
Cuts at: 0 410 543
Size: 410 133
BsiHKAI G_wGCw'C
Cuts at: 0 320 347 543
Size: 320 27 196
Fragments arranged by size:
320 196 27
BsmI GAATG_Cn'
Cuts at: 0 408 543
Size: 408 135
Bsp1286I G_dGCh'C
Cuts at: 0 320 347 497 543
Size: 320 27 150 46
Fragments arranged by size:
320 150 46 27
BspEI T'CCGG_A
Cuts at: 0 530 543
Size: 530 13
BspHI T'CATG_A
Cuts at: 0 320 543
Size: 320 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 481 543
Size: 481 62
BsrDI GCAATG_nn'
Cuts at: 0 288 543
Size: 288 255
BstYI r'GATC_y
Cuts at: 0 218 543
Size: 218 325
BtgI C'CryG_G
Cuts at: 0 164 543
Size: 164 379
DraIII CAC_nnn'GTG
Cuts at: 0 194 543
Size: 194 349
EaeI y'GGCC_r
Cuts at: 0 207 332 407 543
Size: 207 125 75 136
Fragments arranged by size:
207 136 125 75
EagI C'GGCC_G
Cuts at: 0 407 543
Size: 407 136
EarI CTCTTCn'nnn_
Cuts at: 0 28 543
Size: 28 515
EcoICRI GAG'CTC
Cuts at: 0 318 345 543
Size: 318 27 198
Fragments arranged by size:
318 198 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 543
Size: 25 518
EcoO109I rG'GnC_Cy
Cuts at: 0 30 384 543
Size: 30 354 159
Fragments arranged by size:
354 159 30
FspI TGC'GCA
Cuts at: 0 55 543
Size: 55 488
FspAI rTGC'GCAy
Cuts at: 0 55 543
Size: 55 488
HaeII r_GCGC'y
Cuts at: 0 237 543
Size: 237 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 543
Size: 502 32 9
Fragments arranged by size:
502 32 9
HindIII A'AGCT_T
Cuts at: 0 106 543
Size: 106 437
KasI G'GCGC_C
Cuts at: 0 233 543
Size: 233 310
MscI TGG'CCA
Cuts at: 0 209 543
Size: 209 334
MslI CAynn'nnrTG
Cuts at: 0 61 325 543
Size: 61 264 218
Fragments arranged by size:
264 218 61
MspA1I CmG'CkG
Cuts at: 0 485 543
Size: 485 58
NarI GG'CG_CC
Cuts at: 0 234 543
Size: 234 309
PfoI T'CCnGG_A
Cuts at: 0 347 543
Size: 347 196
PpuMI rG'GwC_Cy
Cuts at: 0 384 543
Size: 384 159
PstI C_TGCA'G
Cuts at: 0 84 543
Size: 84 459
SacI G_AGCT'C
Cuts at: 0 320 347 543
Size: 320 27 196
Fragments arranged by size:
320 196 27
SfcI C'TryA_G
Cuts at: 0 80 543
Size: 80 463
SfoI GGC'GCC
Cuts at: 0 235 543
Size: 235 308
SmlI C'TyrA_G
Cuts at: 0 109 543
Size: 109 434
StuI AGG'CCT
Cuts at: 0 260 543
Size: 260 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 193 543
Size: 106 87 350
Fragments arranged by size:
350 106 87
TatI w'GTAC_w
Cuts at: 0 170 543
Size: 170 373
XmnI GAAnn'nnTTC
Cuts at: 0 10 543
Size: 10 533
Enzymes that do cut:
AarI AflII AhdI AlwNI BanI BanII BbeI BbsI
BglI BmgBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstYI
BtgI DraIII EaeI EagI EarI EcoICRI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HindIII KasI MscI MslI
MspA1I NarI PfoI PpuMI PstI SacI SfcI SfoI
SmlI StuI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiWI
BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EciI Eco57MI EcoRI EcoRV FalI FseI
HincII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI