Restriction map for
pF1KB5400
(Linear) (Six-base) MAPSORT of: KIBB5400.seq Check: 1399 from: 1 to: 1413
KIBB5400.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 1225 1413
Size: 1225 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1413
Size: 196 1217
AlwNI CAG_nnn'CTG
Cuts at: 0 1246 1375 1413
Size: 1246 129 38
Fragments arranged by size:
1246 129 38
ApoI r'AATT_y
Cuts at: 0 180 1384 1413
Size: 180 1204 29
Fragments arranged by size:
1204 180 29
BanI G'GyrC_C
Cuts at: 0 953 1046 1195 1225 1413
Size: 953 93 149 30 188
Fragments arranged by size:
953 188 149 93 30
BanII G_rGCy'C
Cuts at: 0 677 1025 1413
Size: 677 348 388
Fragments arranged by size:
677 388 348
BbeI G_GCGC'C
Cuts at: 0 957 1050 1413
Size: 957 93 363
Fragments arranged by size:
957 363 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 300 1413
Size: 208 92 1113
Fragments arranged by size:
1113 208 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1126 1160 1413
Size: 1126 34 253
Fragments arranged by size:
1126 253 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1039 1223 1397 1413
Size: 907 132 184 174 16
Fragments arranged by size:
907 184 174 132 16
Bpu10I CC'TnA_GC
Cuts at: 0 1024 1413
Size: 1024 389
BsaHI Gr'CG_yC
Cuts at: 0 954 1047 1413
Size: 954 93 366
Fragments arranged by size:
954 366 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1218 1248 1413
Size: 1218 30 165
Fragments arranged by size:
1218 165 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1226 1340 1413
Size: 1226 114 73
Fragments arranged by size:
1226 114 73
BseYI C'CCAG_C
Cuts at: 0 1230 1413
Size: 1230 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 970 1388 1413
Size: 890 80 418 25
Fragments arranged by size:
890 418 80 25
BsiHKAI G_wGCw'C
Cuts at: 0 920 1025 1413
Size: 920 105 388
Fragments arranged by size:
920 388 105
BsmI GAATG_Cn'
Cuts at: 0 1357 1413
Size: 1357 56
Bsp1286I G_dGCh'C
Cuts at: 0 677 920 1025 1413
Size: 677 243 105 388
Fragments arranged by size:
677 388 243 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 132 1254 1413
Size: 132 1122 159
Fragments arranged by size:
1122 159 132
BssSI C'ACGA_G
Cuts at: 0 875 1413
Size: 875 538
BstBI TT'CG_AA
Cuts at: 0 456 1413
Size: 456 957
Bsu36I CC'TnA_GG
Cuts at: 0 561 1413
Size: 561 852
BtgI C'CryG_G
Cuts at: 0 402 677 1413
Size: 402 275 736
Fragments arranged by size:
736 402 275
BtsI GCAGTG_nn'
Cuts at: 0 630 1413
Size: 630 783
DraIII CAC_nnn'GTG
Cuts at: 0 403 1413
Size: 403 1010
EaeI y'GGCC_r
Cuts at: 0 513 1413
Size: 513 900
EarI CTCTTCn'nnn_
Cuts at: 0 692 974 1031 1052 1413
Size: 692 282 57 21 361
Fragments arranged by size:
692 361 282 57 21
EcoICRI GAG'CTC
Cuts at: 0 1023 1413
Size: 1023 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 907 1039 1223 1397 1413
Size: 196 711 132 184 174 16
Fragments arranged by size:
711 196 184 174 132 16
EcoO109I rG'GnC_Cy
Cuts at: 0 557 1243 1413
Size: 557 686 170
Fragments arranged by size:
686 557 170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 721 982 1134 1413
Size: 721 261 152 279
Fragments arranged by size:
721 279 261 152
HaeII r_GCGC'y
Cuts at: 0 957 1050 1413
Size: 957 93 363
Fragments arranged by size:
957 363 93
KasI G'GCGC_C
Cuts at: 0 953 1046 1413
Size: 953 93 367
Fragments arranged by size:
953 367 93
KpnI G_GTAC'C
Cuts at: 0 1229 1413
Size: 1229 184
MfeI C'AATT_G
Cuts at: 0 1337 1413
Size: 1337 76
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1413
Size: 503 910
MscI TGG'CCA
Cuts at: 0 515 1413
Size: 515 898
MslI CAynn'nnrTG
Cuts at: 0 826 931 1255 1413
Size: 826 105 324 158
Fragments arranged by size:
826 324 158 105
MspA1I CmG'CkG
Cuts at: 0 414 598 1222 1413
Size: 414 184 624 191
Fragments arranged by size:
624 414 191 184
NarI GG'CG_CC
Cuts at: 0 954 1047 1413
Size: 954 93 366
Fragments arranged by size:
954 366 93
NcoI C'CATG_G
Cuts at: 0 677 1413
Size: 677 736
PflMI CCAn_nnn'nTGG
Cuts at: 0 403 809 1413
Size: 403 406 604
Fragments arranged by size:
604 406 403
PfoI T'CCnGG_A
Cuts at: 0 1239 1374 1413
Size: 1239 135 39
Fragments arranged by size:
1239 135 39
PpuMI rG'GwC_Cy
Cuts at: 0 1243 1413
Size: 1243 170
PsiI TTA'TAA
Cuts at: 0 724 1413
Size: 724 689
PvuII CAG'CTG
Cuts at: 0 414 598 1222 1413
Size: 414 184 624 191
Fragments arranged by size:
624 414 191 184
SacI G_AGCT'C
Cuts at: 0 1025 1413
Size: 1025 388
SapI GCTCTTCn'nnn_
Cuts at: 0 974 1031 1413
Size: 974 57 382
Fragments arranged by size:
974 382 57
SexAI A'CCwGG_T
Cuts at: 0 920 1413
Size: 920 493
SfcI C'TryA_G
Cuts at: 0 530 1413
Size: 530 883
SfoI GGC'GCC
Cuts at: 0 955 1048 1413
Size: 955 93 365
Fragments arranged by size:
955 365 93
StyI C'CwwG_G
Cuts at: 0 677 1413
Size: 677 736
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 777 1413
Size: 226 551 636
Fragments arranged by size:
636 551 226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 486 1413
Size: 381 105 927
Fragments arranged by size:
927 381 105
XmnI GAAnn'nnTTC
Cuts at: 0 205 965 1413
Size: 205 760 448
Fragments arranged by size:
760 448 205
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI BanI BanII BbeI BbsI
BcgI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BssSI BstBI Bsu36I BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII KasI KpnI MfeI MmeI MscI MslI MspA1I
NarI NcoI PflMI PfoI PpuMI PsiI PvuII SacI
SapI SexAI SfcI SfoI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI