Restriction map for
pF1KB5401
(Linear) (Six-base) MAPSORT of: KIBB5401.seq Check: 3986 from: 1 to: 789
KIBB5401.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 789
Size: 375 414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 789
Size: 380 409
AlwNI CAG_nnn'CTG
Cuts at: 0 206 324 789
Size: 206 118 465
Fragments arranged by size:
465 206 118
ApaI G_GGCC'C
Cuts at: 0 239 789
Size: 239 550
BanII G_rGCy'C
Cuts at: 0 239 789
Size: 239 550
BbsI GAAGACnn'nnnn_
Cuts at: 0 706 789
Size: 706 83
BclI T'GATC_A
Cuts at: 0 536 612 789
Size: 536 76 177
Fragments arranged by size:
536 177 76
Bme1580I G_kGCm'C
Cuts at: 0 239 789
Size: 239 550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 330 789
Size: 330 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 713 789
Size: 86 627 76
Fragments arranged by size:
627 86 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 789
Size: 301 488
BsaAI yAC'GTr
Cuts at: 0 513 789
Size: 513 276
BsaBI GATnn'nnATC
Cuts at: 0 451 535 789
Size: 451 84 254
Fragments arranged by size:
451 254 84
BsaHI Gr'CG_yC
Cuts at: 0 266 789
Size: 266 523
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 789
Size: 97 30 662
Fragments arranged by size:
662 97 30
BseYI C'CCAG_C
Cuts at: 0 516 789
Size: 516 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 403 457 789
Size: 392 11 54 332
Fragments arranged by size:
392 332 54 11
BsmI GAATG_Cn'
Cuts at: 0 22 789
Size: 22 767
Bsp1286I G_dGCh'C
Cuts at: 0 239 789
Size: 239 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 375 789
Size: 302 73 414
Fragments arranged by size:
414 302 73
BsrBI CCG'CTC
Cuts at: 0 161 415 789
Size: 161 254 374
Fragments arranged by size:
374 254 161
BsrDI GCAATG_nn'
Cuts at: 0 542 789
Size: 542 247
BsrFI r'CCGG_y
Cuts at: 0 190 262 789
Size: 190 72 527
Fragments arranged by size:
527 190 72
BstYI r'GATC_y
Cuts at: 0 61 789
Size: 61 728
Bsu36I CC'TnA_GG
Cuts at: 0 740 789
Size: 740 49
EaeI y'GGCC_r
Cuts at: 0 87 789
Size: 87 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 380 713 789
Size: 86 294 333 76
Fragments arranged by size:
333 294 86 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 582 789
Size: 582 207
EcoO109I rG'GnC_Cy
Cuts at: 0 225 236 291 321 492 789
Size: 225 11 55 30 171 297
Fragments arranged by size:
297 225 171 55 30 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 545 577 789
Size: 97 32 416 32 212
Fragments arranged by size:
416 212 97 32 32
HincII GTy'rAC
Cuts at: 0 735 789
Size: 735 54
MslI CAynn'nnrTG
Cuts at: 0 526 532 789
Size: 526 6 257
Fragments arranged by size:
526 257 6
MspA1I CmG'CkG
Cuts at: 0 134 433 462 789
Size: 134 299 29 327
Fragments arranged by size:
327 299 134 29
NaeI GCC'GGC
Cuts at: 0 192 264 789
Size: 192 72 525
Fragments arranged by size:
525 192 72
NgoMIV G'CCGG_C
Cuts at: 0 190 262 789
Size: 190 72 527
Fragments arranged by size:
527 190 72
NspI r_CATG'y
Cuts at: 0 42 789
Size: 42 747
PflMI CCAn_nnn'nTGG
Cuts at: 0 448 789
Size: 448 341
PfoI T'CCnGG_A
Cuts at: 0 347 789
Size: 347 442
PpuMI rG'GwC_Cy
Cuts at: 0 225 291 492 789
Size: 225 66 201 297
Fragments arranged by size:
297 225 201 66
PspOMI G'GGCC_C
Cuts at: 0 235 789
Size: 235 554
PvuII CAG'CTG
Cuts at: 0 433 789
Size: 433 356
StyI C'CwwG_G
Cuts at: 0 479 789
Size: 479 310
Enzymes that do cut:
AarI AcuI AlwNI ApaI BanII BbsI BclI Bme1580I
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI
Bsu36I EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MslI
MspA1I NaeI NgoMIV NspI PflMI PfoI PpuMI PspOMI
PvuII StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaWI BseRI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI