Restriction map for
pF1KB5407
(Linear) (Six-base) MAPSORT of: KIBB5407.seq Check: 6492 from: 1 to: 1482
KIBB5407.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 612 1482
Size: 90 522 870
Fragments arranged by size:
870 522 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1423 1482
Size: 1423 59
AleI CACnn'nnGTG
Cuts at: 0 824 1482
Size: 824 658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 842 1482
Size: 842 640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 1482
Size: 346 32 1104
Fragments arranged by size:
1104 346 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 627 873 1366 1482
Size: 105 522 246 493 116
Fragments arranged by size:
522 493 246 116 105
AvaI C'yCGr_G
Cuts at: 0 523 613 921 1482
Size: 523 90 308 561
Fragments arranged by size:
561 523 308 90
BanI G'GyrC_C
Cuts at: 0 482 604 826 1380 1482
Size: 482 122 222 554 102
Fragments arranged by size:
554 482 222 122 102
BanII G_rGCy'C
Cuts at: 0 49 155 1333 1482
Size: 49 106 1178 149
Fragments arranged by size:
1178 149 106 49
BbeI G_GCGC'C
Cuts at: 0 486 1384 1482
Size: 486 898 98
Fragments arranged by size:
898 486 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 1002 1482
Size: 1002 480
BglI GCCn_nnn'nGGC
Cuts at: 0 868 1482
Size: 868 614
Bme1580I G_kGCm'C
Cuts at: 0 538 607 831 1482
Size: 538 69 224 651
Fragments arranged by size:
651 538 224 69
BmgBI CAC'GTC
Cuts at: 0 777 1482
Size: 777 705
BmrI ACTGGGnnnn_n'
Cuts at: 0 979 1068 1482
Size: 979 89 414
Fragments arranged by size:
979 414 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 535 685 724 1276 1284 1300 1343
Size: 433 102 150 39 552 8 16 43
Cuts at: 1343 1453 1482
Size: 110 29
Fragments arranged by size:
552 433 150 110 102 43 39 29
16 8
Bpu10I CC'TnA_GC
Cuts at: 0 391 1482
Size: 391 1091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 934 1482
Size: 343 591 548
Fragments arranged by size:
591 548 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 1045 1252 1482
Size: 130 915 207 230
Fragments arranged by size:
915 230 207 130
BsaAI yAC'GTr
Cuts at: 0 801 1482
Size: 801 681
BsaHI Gr'CG_yC
Cuts at: 0 251 483 632 1381 1482
Size: 251 232 149 749 101
Fragments arranged by size:
749 251 232 149 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1482
Size: 667 30 785
Fragments arranged by size:
785 667 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 421 430 909 1459 1482
Size: 109 312 9 479 550 23
Fragments arranged by size:
550 479 312 109 23 9
BseYI C'CCAG_C
Cuts at: 0 169 1143 1482
Size: 169 974 339
Fragments arranged by size:
974 339 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 727 874 886 1482
Size: 454 273 147 12 596
Fragments arranged by size:
596 454 273 147 12
BsiEI CG_ry'CG
Cuts at: 0 470 1482
Size: 470 1012
BsiHKAI G_wGCw'C
Cuts at: 0 49 155 560 656 1482
Size: 49 106 405 96 826
Fragments arranged by size:
826 405 106 96 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 585 670 1482
Size: 585 85 812
Fragments arranged by size:
812 585 85
Bsp1286I G_dGCh'C
Cuts at: 0 49 155 538 560 607 656 831 1333
Size: 49 106 383 22 47 49 175 502
Cuts at: 1333 1482
Size: 149
Fragments arranged by size:
502 383 175 149 106 49 49 47
22
BspHI T'CATG_A
Cuts at: 0 761 1482
Size: 761 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 612 719 1482
Size: 90 522 107 763
Fragments arranged by size:
763 522 107 90
BsrFI r'CCGG_y
Cuts at: 0 1397 1482
Size: 1397 85
BssHII G'CGCG_C
Cuts at: 0 424 892 1482
Size: 424 468 590
Fragments arranged by size:
590 468 424
BssSI C'ACGA_G
Cuts at: 0 1273 1482
Size: 1273 209
BstAPI GCAn_nnn'nTGC
Cuts at: 0 864 873 1482
Size: 864 9 609
Fragments arranged by size:
864 609 9
BtgI C'CryG_G
Cuts at: 0 587 1031 1262 1482
Size: 587 444 231 220
Fragments arranged by size:
587 444 231 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 873 1170 1176 1482
Size: 873 297 6 306
Fragments arranged by size:
873 306 297 6
BtsI GCAGTG_nn'
Cuts at: 0 1001 1344 1482
Size: 1001 343 138
Fragments arranged by size:
1001 343 138
EaeI y'GGCC_r
Cuts at: 0 467 551 782 1160 1265 1482
Size: 467 84 231 378 105 217
Fragments arranged by size:
467 378 231 217 105 84
EagI C'GGCC_G
Cuts at: 0 467 1482
Size: 467 1015
EarI CTCTTCn'nnn_
Cuts at: 0 727 1482
Size: 727 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 1482
Size: 163 1319
EcoICRI GAG'CTC
Cuts at: 0 47 153 1482
Size: 47 106 1329
Fragments arranged by size:
1329 106 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 535 685 724 1276 1284 1300 1343
Size: 433 102 150 39 552 8 16 43
Cuts at: 1343 1423 1453 1482
Size: 80 30 29
Fragments arranged by size:
552 433 150 102 80 43 39 30
29 16 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1441 1482
Size: 1441 41
EcoO109I rG'GnC_Cy
Cuts at: 0 114 243 1460 1482
Size: 114 129 1217 22
Fragments arranged by size:
1217 129 114 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 762 975 1482
Size: 762 213 507
Fragments arranged by size:
762 507 213
FspI TGC'GCA
Cuts at: 0 886 1482
Size: 886 596
FspAI rTGC'GCAy
Cuts at: 0 886 1482
Size: 886 596
HaeII r_GCGC'y
Cuts at: 0 486 874 1384 1482
Size: 486 388 510 98
Fragments arranged by size:
510 486 388 98
HincII GTy'rAC
Cuts at: 0 81 1482
Size: 81 1401
KasI G'GCGC_C
Cuts at: 0 482 1380 1482
Size: 482 898 102
Fragments arranged by size:
898 482 102
MscI TGG'CCA
Cuts at: 0 784 1482
Size: 784 698
MslI CAynn'nnrTG
Cuts at: 0 271 598 824 1099 1482
Size: 271 327 226 275 383
Fragments arranged by size:
383 327 275 271 226
MspA1I CmG'CkG
Cuts at: 0 52 479 488 1264 1307 1482
Size: 52 427 9 776 43 175
Fragments arranged by size:
776 427 175 52 43 9
NaeI GCC'GGC
Cuts at: 0 1399 1482
Size: 1399 83
NarI GG'CG_CC
Cuts at: 0 483 1381 1482
Size: 483 898 101
Fragments arranged by size:
898 483 101
NcoI C'CATG_G
Cuts at: 0 1031 1482
Size: 1031 451
NgoMIV G'CCGG_C
Cuts at: 0 1397 1482
Size: 1397 85
PasI CC'CwG_GG
Cuts at: 0 1020 1482
Size: 1020 462
PfoI T'CCnGG_A
Cuts at: 0 26 365 443 512 1253 1482
Size: 26 339 78 69 741 229
Fragments arranged by size:
741 339 229 78 69 26
PpuMI rG'GwC_Cy
Cuts at: 0 114 243 1460 1482
Size: 114 129 1217 22
Fragments arranged by size:
1217 129 114 22
PshAI GACnn'nnGTC
Cuts at: 0 1108 1482
Size: 1108 374
PstI C_TGCA'G
Cuts at: 0 623 932 1393 1482
Size: 623 309 461 89
Fragments arranged by size:
623 461 309 89
SacI G_AGCT'C
Cuts at: 0 49 155 1482
Size: 49 106 1327
Fragments arranged by size:
1327 106 49
SacII CC_GC'GG
Cuts at: 0 1265 1482
Size: 1265 217
SanDI GG'GwC_CC
Cuts at: 0 243 1482
Size: 243 1239
SfcI C'TryA_G
Cuts at: 0 619 928 1389 1482
Size: 619 309 461 93
Fragments arranged by size:
619 461 309 93
SfoI GGC'GCC
Cuts at: 0 484 1382 1482
Size: 484 898 100
Fragments arranged by size:
898 484 100
SmlI C'TyrA_G
Cuts at: 0 358 949 1482
Size: 358 591 533
Fragments arranged by size:
591 533 358
StyI C'CwwG_G
Cuts at: 0 1031 1482
Size: 1031 451
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 747 814 1456 1482
Size: 747 67 642 26
Fragments arranged by size:
747 642 67 26
TatI w'GTAC_w
Cuts at: 0 275 1482
Size: 275 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 1482
Size: 667 32 783
Fragments arranged by size:
783 667 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1140 1275 1482
Size: 1140 135 207
Fragments arranged by size:
1140 207 135
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI
BssHII BssSI BstAPI BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII HincII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PfoI PpuMI PshAI PstI
SacI SacII SanDI SfcI SfoI SmlI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI
BsrBI BsrDI BsrGI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV
FseI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI