Restriction map for
pF1KB5414
(Linear) (Six-base) MAPSORT of: KIBB5414.seq Check: 9092 from: 1 to: 1023
KIBB5414.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 495 1023
Size: 495 528
Acc65I G'GTAC_C
Cuts at: 0 589 1023
Size: 589 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 488 857 1023
Size: 464 24 369 166
Fragments arranged by size:
464 369 166 24
AflII C'TTAA_G
Cuts at: 0 994 1023
Size: 994 29
AlwNI CAG_nnn'CTG
Cuts at: 0 58 487 609 1023
Size: 58 429 122 414
Fragments arranged by size:
429 414 122 58
ApoI r'AATT_y
Cuts at: 0 377 407 1023
Size: 377 30 616
Fragments arranged by size:
616 377 30
AvaI C'yCGr_G
Cuts at: 0 890 1023
Size: 890 133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 573 606 1023
Size: 573 33 417
Fragments arranged by size:
573 417 33
BanI G'GyrC_C
Cuts at: 0 507 589 1023
Size: 507 82 434
Fragments arranged by size:
507 434 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 1023
Size: 254 769
BglI GCCn_nnn'nGGC
Cuts at: 0 180 1023
Size: 180 843
BglII A'GATC_T
Cuts at: 0 695 1023
Size: 695 328
Bme1580I G_kGCm'C
Cuts at: 0 707 1023
Size: 707 316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 758 812 1023
Size: 35 723 54 211
Fragments arranged by size:
723 211 54 35
BsaWI w'CCGG_w
Cuts at: 0 292 1023
Size: 292 731
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 578 994 1023
Size: 487 91 416 29
Fragments arranged by size:
487 416 91 29
BseYI C'CCAG_C
Cuts at: 0 86 218 706 813 1023
Size: 86 132 488 107 210
Fragments arranged by size:
488 210 132 107 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 734 1023
Size: 734 289
Bsp1286I G_dGCh'C
Cuts at: 0 707 1023
Size: 707 316
BspEI T'CCGG_A
Cuts at: 0 292 1023
Size: 292 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 495 1023
Size: 495 528
BstAPI GCAn_nnn'nTGC
Cuts at: 0 133 1023
Size: 133 890
BstXI CCAn_nnnn'nTGG
Cuts at: 0 34 1023
Size: 34 989
BstYI r'GATC_y
Cuts at: 0 695 1023
Size: 695 328
BtgI C'CryG_G
Cuts at: 0 5 198 1023
Size: 5 193 825
Fragments arranged by size:
825 193 5
BtsI GCAGTG_nn'
Cuts at: 0 540 953 1023
Size: 540 413 70
Fragments arranged by size:
540 413 70
EarI CTCTTCn'nnn_
Cuts at: 0 452 464 1023
Size: 452 12 559
Fragments arranged by size:
559 452 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 464 488 758 812 857 1023
Size: 35 429 24 270 54 45 166
Fragments arranged by size:
429 270 166 54 45 35 24
EcoO109I rG'GnC_Cy
Cuts at: 0 606 999 1023
Size: 606 393 24
Fragments arranged by size:
606 393 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 410 1023
Size: 410 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 1023
Size: 680 32 311
Fragments arranged by size:
680 311 32
KpnI G_GTAC'C
Cuts at: 0 593 1023
Size: 593 430
MspA1I CmG'CkG
Cuts at: 0 46 171 576 1023
Size: 46 125 405 447
Fragments arranged by size:
447 405 125 46
NcoI C'CATG_G
Cuts at: 0 5 198 1023
Size: 5 193 825
Fragments arranged by size:
825 193 5
PasI CC'CwG_GG
Cuts at: 0 558 1023
Size: 558 465
PpuMI rG'GwC_Cy
Cuts at: 0 999 1023
Size: 999 24
PvuII CAG'CTG
Cuts at: 0 46 171 576 1023
Size: 46 125 405 447
Fragments arranged by size:
447 405 125 46
SfcI C'TryA_G
Cuts at: 0 399 664 1023
Size: 399 265 359
Fragments arranged by size:
399 359 265
SmlI C'TyrA_G
Cuts at: 0 890 994 1023
Size: 890 104 29
Fragments arranged by size:
890 104 29
StuI AGG'CCT
Cuts at: 0 393 1023
Size: 393 630
StyI C'CwwG_G
Cuts at: 0 5 198 445 1023
Size: 5 193 247 578
Fragments arranged by size:
578 247 193 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 786 1023
Size: 786 237
TatI w'GTAC_w
Cuts at: 0 165 1023
Size: 165 858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 532 1023
Size: 532 491
XhoI C'TCGA_G
Cuts at: 0 890 1023
Size: 890 133
Enzymes that do cut:
AarI Acc65I AcuI AflII AlwNI ApoI AvaI BaeI
BanI BbsI BglI BglII Bme1580I BpmI BsaWI BseRI
BseYI BsmBI Bsp1286I BspEI BspMI BstAPI BstXI BstYI
BtgI BtsI EarI Eco57MI EcoO109I FalI Hin4I KpnI
MspA1I NcoI PasI PpuMI PvuII SfcI SmlI StuI
StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MmeI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XmaI XmnI ZraI