(Linear) (Six-base) MAPSORT of: KIBB5437.seq  Check: 4175  from: 1  to: 999



KIBB5437.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      22     350     391     655     999 
   Size:         22     328      41     264     344

  Fragments arranged by size: 

                 344     328     264      41      22
 
ApoI r'AATT_y

Cuts at:      0     502     999 
   Size:        502     497
 
BclI T'GATC_A

Cuts at:      0     467     999 
   Size:        467     532
 
BglII A'GATC_T

Cuts at:      0     848     999 
   Size:        848     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     999 
   Size:        566     433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     999 
   Size:        431     568
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     999 
   Size:        109     890
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     999 
   Size:        350     649
 
BsiHKAI G_wGCw'C

Cuts at:      0     604     999 
   Size:        604     395
 
Bsp1286I G_dGCh'C

Cuts at:      0     604     999 
   Size:        604     395
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      22     999 
   Size:         22     977
 
BstYI r'GATC_y

Cuts at:      0     848     999 
   Size:        848     151
 
BtsI GCAGTG_nn'

Cuts at:      0     236     999 
   Size:        236     763
 
DraI TTT'AAA

Cuts at:      0     609     830     999 
   Size:        609     221     169

  Fragments arranged by size: 

                 609     221     169
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     408     999 
   Size:        408     591
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     999 
   Size:        566     433
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     999 
   Size:         75     924
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     791     999 
   Size:        791     208
 
MslI CAynn'nnrTG

Cuts at:      0     241     999 
   Size:        241     758
 
PpuMI rG'GwC_Cy

Cuts at:      0      75     999 
   Size:         75     924
 
PstI C_TGCA'G

Cuts at:      0      15     999 
   Size:         15     984
 
ScaI AGT'ACT

Cuts at:      0     614     999 
   Size:        614     385
 
SfcI C'TryA_G

Cuts at:      0      11     999 
   Size:         11     988
 
SmlI C'TyrA_G

Cuts at:      0     446     999 
   Size:        446     553
 
TatI w'GTAC_w

Cuts at:      0     612     999 
   Size:        612     387
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     168     200     999 
   Size:        168      32     799

  Fragments arranged by size: 

                 799     168      32
 
XbaI T'CTAG_A

Cuts at:      0     213     999 
   Size:        213     786
 
XmnI GAAnn'nnTTC

Cuts at:      0     575     999 
   Size:        575     424
 

 Enzymes that do cut:

    AlwNI     ApoI     BclI    BglII     BpmI    BpuEI    BseRI     BsgI
  BsiHKAI Bsp1286I   BstAPI    BstYI     BtsI     DraI     DrdI  Eco57MI
 EcoO109I     MmeI     MslI    PpuMI     PstI     ScaI     SfcI     SmlI
     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI     ZraI