Restriction map for
pF1KB5438
(Linear) (Six-base) MAPSORT of: KIBB5438.seq Check: 4518 from: 1 to: 1326
KIBB5438.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AatII G_ACGT'C
Cuts at: 0 341 1326
Size: 341 985
AccI GT'mk_AC
Cuts at: 0 275 569 620 1326
Size: 275 294 51 706
Fragments arranged by size:
706 294 275 51
AleI CACnn'nnGTG
Cuts at: 0 400 1326
Size: 400 926
AlwNI CAG_nnn'CTG
Cuts at: 0 831 1326
Size: 831 495
ApaI G_GGCC'C
Cuts at: 0 699 1326
Size: 699 627
ApaLI G'TGCA_C
Cuts at: 0 348 1326
Size: 348 978
ApoI r'AATT_y
Cuts at: 0 298 1326
Size: 298 1028
AvaI C'yCGr_G
Cuts at: 0 736 865 1326
Size: 736 129 461
Fragments arranged by size:
736 461 129
BamHI G'GATC_C
Cuts at: 0 3 1326
Size: 3 1323
BanII G_rGCy'C
Cuts at: 0 699 711 825 1326
Size: 699 12 114 501
Fragments arranged by size:
699 501 114 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 138 1326
Size: 138 1188
BglI GCCn_nnn'nGGC
Cuts at: 0 556 1326
Size: 556 770
BglII A'GATC_T
Cuts at: 0 629 1013 1326
Size: 629 384 313
Fragments arranged by size:
629 384 313
Bme1580I G_kGCm'C
Cuts at: 0 352 599 699 1326
Size: 352 247 100 627
Fragments arranged by size:
627 352 247 100
BmrI ACTGGGnnnn_n'
Cuts at: 0 781 1326
Size: 781 545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 864 1326
Size: 864 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 72 301 834 853 1326
Size: 58 14 229 533 19 473
Fragments arranged by size:
533 473 229 58 19 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1326
Size: 1045 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 943 1326
Size: 943 383
BsaAI yAC'GTr
Cuts at: 0 594 1232 1326
Size: 594 638 94
Fragments arranged by size:
638 594 94
BsaBI GATnn'nnATC
Cuts at: 0 32 466 1326
Size: 32 434 860
Fragments arranged by size:
860 434 32
BsaHI Gr'CG_yC
Cuts at: 0 338 651 1326
Size: 338 313 675
Fragments arranged by size:
675 338 313
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 496 564 1326
Size: 232 264 68 762
Fragments arranged by size:
762 264 232 68
BseYI C'CCAG_C
Cuts at: 0 895 1088 1236 1326
Size: 895 193 148 90
Fragments arranged by size:
895 193 148 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1326
Size: 738 588
BsiHKAI G_wGCw'C
Cuts at: 0 352 1326
Size: 352 974
BsmBI CGTCTCn'nnnn_
Cuts at: 0 588 1326
Size: 588 738
Bsp1286I G_dGCh'C
Cuts at: 0 352 599 699 711 825 1326
Size: 352 247 100 12 114 501
Fragments arranged by size:
501 352 247 114 100 12
BspHI T'CATG_A
Cuts at: 0 341 458 764 1326
Size: 341 117 306 562
Fragments arranged by size:
562 341 306 117
BsrDI GCAATG_nn'
Cuts at: 0 545 1326
Size: 545 781
BsrFI r'CCGG_y
Cuts at: 0 56 1326
Size: 56 1270
BsrGI T'GTAC_A
Cuts at: 0 419 1217 1326
Size: 419 798 109
Fragments arranged by size:
798 419 109
BstEII G'GTnAC_C
Cuts at: 0 450 1326
Size: 450 876
BstYI r'GATC_y
Cuts at: 0 3 629 1013 1022 1313 1326
Size: 3 626 384 9 291 13
Fragments arranged by size:
626 384 291 13 9 3
Bsu36I CC'TnA_GG
Cuts at: 0 703 1326
Size: 703 623
BtgI C'CryG_G
Cuts at: 0 933 1326
Size: 933 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 1326
Size: 117 1209
BtsI GCAGTG_nn'
Cuts at: 0 115 236 1320 1326
Size: 115 121 1084 6
Fragments arranged by size:
1084 121 115 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 244 325 1326
Size: 244 81 1001
Fragments arranged by size:
1001 244 81
EaeI y'GGCC_r
Cuts at: 0 233 404 1326
Size: 233 171 922
Fragments arranged by size:
922 233 171
EarI CTCTTCn'nnn_
Cuts at: 0 514 1326
Size: 514 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 813 1326
Size: 100 713 513
Fragments arranged by size:
713 513 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 72 301 834 853 1326
Size: 58 14 229 533 19 473
Fragments arranged by size:
533 473 229 58 19 14
EcoO109I rG'GnC_Cy
Cuts at: 0 477 695 872 1326
Size: 477 218 177 454
Fragments arranged by size:
477 454 218 177
HaeII r_GCGC'y
Cuts at: 0 193 447 1226 1256 1326
Size: 193 254 779 30 70
Fragments arranged by size:
779 254 193 70 30
HincII GTy'rAC
Cuts at: 0 666 1248 1326
Size: 666 582 78
Fragments arranged by size:
666 582 78
MscI TGG'CCA
Cuts at: 0 406 1326
Size: 406 920
MslI CAynn'nnrTG
Cuts at: 0 172 400 412 457 1114 1326
Size: 172 228 12 45 657 212
Fragments arranged by size:
657 228 212 172 45 12
MspA1I CmG'CkG
Cuts at: 0 400 909 1326
Size: 400 509 417
Fragments arranged by size:
509 417 400
NaeI GCC'GGC
Cuts at: 0 58 1326
Size: 58 1268
NgoMIV G'CCGG_C
Cuts at: 0 56 1326
Size: 56 1270
NspI r_CATG'y
Cuts at: 0 988 1326
Size: 988 338
PasI CC'CwG_GG
Cuts at: 0 266 1326
Size: 266 1060
PflMI CCAn_nnn'nTGG
Cuts at: 0 473 831 1326
Size: 473 358 495
Fragments arranged by size:
495 473 358
PmlI CAC'GTG
Cuts at: 0 1232 1326
Size: 1232 94
PpuMI rG'GwC_Cy
Cuts at: 0 477 872 1326
Size: 477 395 454
Fragments arranged by size:
477 454 395
PspOMI G'GGCC_C
Cuts at: 0 695 1326
Size: 695 631
PstI C_TGCA'G
Cuts at: 0 197 1326
Size: 197 1129
PvuII CAG'CTG
Cuts at: 0 400 909 1326
Size: 400 509 417
Fragments arranged by size:
509 417 400
SfcI C'TryA_G
Cuts at: 0 193 432 1326
Size: 193 239 894
Fragments arranged by size:
894 239 193
SmlI C'TyrA_G
Cuts at: 0 1060 1326
Size: 1060 266
SphI G_CATG'C
Cuts at: 0 988 1326
Size: 988 338
StuI AGG'CCT
Cuts at: 0 1303 1326
Size: 1303 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 909 1326
Size: 909 417
TatI w'GTAC_w
Cuts at: 0 419 727 1217 1326
Size: 419 308 490 109
Fragments arranged by size:
490 419 308 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 1326
Size: 300 32 994
Fragments arranged by size:
994 300 32
ZraI GAC'GTC
Cuts at: 0 339 1326
Size: 339 987
Enzymes that do cut:
AatII AccI AleI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanII BbsI BglI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI EciI Eco57MI EcoO109I HaeII HincII MscI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PmlI PpuMI
PspOMI PstI PvuII SfcI SmlI SphI StuI TaqII
TatI TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI Bpu10I BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI