(Linear) (Six-base) MAPSORT of: KIBB5439.seq  Check: 168  from: 1  to: 621



KIBB5439.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     621 
   Size:         46     575
 
AflII C'TTAA_G

Cuts at:      0     520     621 
   Size:        520     101
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     264     621 
   Size:        264     357
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     478     510     621 
   Size:        478      32     111

  Fragments arranged by size: 

                 478     111      32
 
ApoI r'AATT_y

Cuts at:      0      94     553     621 
   Size:         94     459      68

  Fragments arranged by size: 

                 459      94      68
 
AvaI C'yCGr_G

Cuts at:      0     335     621 
   Size:        335     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      67     621 
   Size:         67     554
 
BglI GCCn_nnn'nGGC

Cuts at:      0     472     621 
   Size:        472     149
 
Bme1580I G_kGCm'C

Cuts at:      0     417     621 
   Size:        417     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     324     621 
   Size:         71     253     297

  Fragments arranged by size: 

                 297     253      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     451     621 
   Size:        451     170
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     478     508     621 
   Size:        478      30     113

  Fragments arranged by size: 

                 478     113      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     621 
   Size:        260     361
 
BsmI GAATG_Cn'

Cuts at:      0     519     621 
   Size:        519     102
 
Bsp1286I G_dGCh'C

Cuts at:      0     417     621 
   Size:        417     204
 
BspHI T'CATG_A

Cuts at:      0      71     621 
   Size:         71     550
 
BsrGI T'GTAC_A

Cuts at:      0     545     621 
   Size:        545      76
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     514     621 
   Size:        514     107
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     483     621 
   Size:        483     138
 
BstYI r'GATC_y

Cuts at:      0     339     621 
   Size:        339     282
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     166     621 
   Size:        166     455
 
EaeI y'GGCC_r

Cuts at:      0     401     621 
   Size:        401     220
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46      71     324     621 
   Size:         46      25     253     297

  Fragments arranged by size: 

                 297     253      46      25
 
MscI TGG'CCA

Cuts at:      0     403     621 
   Size:        403     218
 
MslI CAynn'nnrTG

Cuts at:      0     481     621 
   Size:        481     140
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510     621 
   Size:        478      32     111

  Fragments arranged by size: 

                 478     111      32
 
PstI C_TGCA'G

Cuts at:      0     616     621 
   Size:        616       5
 
SfcI C'TryA_G

Cuts at:      0     231     429     612     621 
   Size:        231     198     183       9

  Fragments arranged by size: 

                 231     198     183       9
 
SmlI C'TyrA_G

Cuts at:      0     520     621 
   Size:        520     101
 
StuI AGG'CCT

Cuts at:      0     421     598     621 
   Size:        421     177      23

  Fragments arranged by size: 

                 421     177      23
 
StyI C'CwwG_G

Cuts at:      0     140     467     593     621 
   Size:        140     327     126      28

  Fragments arranged by size: 

                 327     140     126      28
 
TatI w'GTAC_w

Cuts at:      0     107     545     621 
   Size:        107     438      76

  Fragments arranged by size: 

                 438     107      76
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147     621 
   Size:        147     474
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     AloI     ApoI     AvaI     BbsI     BglI
 Bme1580I     BpmI     BsaI    BsaXI     BsgI     BsmI Bsp1286I    BspHI
    BsrGI   BstAPI    BstXI    BstYI     DrdI     EaeI  Eco57MI     MscI
     MslI     PpiI     PstI     SfcI     SmlI     StuI     StyI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI