Restriction map for
pF1KB5440
(Linear) (Six-base) MAPSORT of: KIBB5440.seq Check: 9270 from: 1 to: 981
KIBB5440.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 779 981
Size: 779 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 981
Size: 393 588
AfeI AGC'GCT
Cuts at: 0 428 981
Size: 428 553
AhdI GACnn_n'nnGTC
Cuts at: 0 48 981
Size: 48 933
AleI CACnn'nnGTG
Cuts at: 0 966 981
Size: 966 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 981
Size: 628 353
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 673 705 981
Size: 673 32 276
Fragments arranged by size:
673 276 32
ApaI G_GGCC'C
Cuts at: 0 211 274 545 915 981
Size: 211 63 271 370 66
Fragments arranged by size:
370 271 211 66 63
ApaLI G'TGCA_C
Cuts at: 0 969 981
Size: 969 12
AvaI C'yCGr_G
Cuts at: 0 173 204 735 981
Size: 173 31 531 246
Fragments arranged by size:
531 246 173 31
BanI G'GyrC_C
Cuts at: 0 294 374 981
Size: 294 80 607
Fragments arranged by size:
607 294 80
BanII G_rGCy'C
Cuts at: 0 211 274 323 545 569 719 915 981
Size: 211 63 49 222 24 150 196 66
Fragments arranged by size:
222 211 196 150 66 63 49 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 84 263 321 981
Size: 84 179 58 660
Fragments arranged by size:
660 179 84 58
BlpI GC'TnA_GC
Cuts at: 0 151 981
Size: 151 830
Bme1580I G_kGCm'C
Cuts at: 0 211 274 299 338 545 915 973 981
Size: 211 63 25 39 207 370 58 8
Fragments arranged by size:
370 211 207 63 58 39 25 8
BmgBI CAC'GTC
Cuts at: 0 168 981
Size: 168 813
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 758 981
Size: 258 500 223
Fragments arranged by size:
500 258 223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 981
Size: 733 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 703 981
Size: 469 234 278
Fragments arranged by size:
469 278 234
Bpu10I CC'TnA_GC
Cuts at: 0 242 355 981
Size: 242 113 626
Fragments arranged by size:
626 242 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 981
Size: 193 788
BsaAI yAC'GTr
Cuts at: 0 387 552 981
Size: 387 165 429
Fragments arranged by size:
429 387 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 981
Size: 541 30 410
Fragments arranged by size:
541 410 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 526 981
Size: 331 195 455
Fragments arranged by size:
455 331 195
BseYI C'CCAG_C
Cuts at: 0 120 538 668 712 780 981
Size: 120 418 130 44 68 201
Fragments arranged by size:
418 201 130 120 68 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 981
Size: 903 78
BsiHKAI G_wGCw'C
Cuts at: 0 248 323 392 569 973 981
Size: 248 75 69 177 404 8
Fragments arranged by size:
404 248 177 75 69 8
Bsp1286I G_dGCh'C
Cuts at: 0 211 248 274 299 323 338 392 545
Size: 211 37 26 25 24 15 54 153
Cuts at: 545 569 719 915 973 981
Size: 24 150 196 58 8
Fragments arranged by size:
211 196 153 150 58 54 37 26
25 24 24 15 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 503 779 981
Size: 38 465 276 202
Fragments arranged by size:
465 276 202 38
BsrDI GCAATG_nn'
Cuts at: 0 473 648 981
Size: 473 175 333
Fragments arranged by size:
473 333 175
BsrFI r'CCGG_y
Cuts at: 0 632 695 981
Size: 632 63 286
Fragments arranged by size:
632 286 63
BstYI r'GATC_y
Cuts at: 0 368 981
Size: 368 613
Bsu36I CC'TnA_GG
Cuts at: 0 533 981
Size: 533 448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 795 981
Size: 795 186
BtsI GCAGTG_nn'
Cuts at: 0 293 890 981
Size: 293 597 91
Fragments arranged by size:
597 293 91
EarI CTCTTCn'nnn_
Cuts at: 0 787 981
Size: 787 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 804 981
Size: 718 86 177
Fragments arranged by size:
718 177 86
EcoICRI GAG'CTC
Cuts at: 0 321 567 981
Size: 321 246 414
Fragments arranged by size:
414 321 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 469 703 981
Size: 393 76 234 278
Fragments arranged by size:
393 278 234 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12 981
Size: 12 969
EcoO109I rG'GnC_Cy
Cuts at: 0 208 270 271 542 911 912 981
Size: 208 62 1 271 369 1 69
Fragments arranged by size:
369 271 208 69 62 1 1
HaeII r_GCGC'y
Cuts at: 0 430 981
Size: 430 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 981
Size: 34 32 915
Fragments arranged by size:
915 34 32
HincII GTy'rAC
Cuts at: 0 492 981
Size: 492 489
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 920 981
Size: 677 243 61
Fragments arranged by size:
677 243 61
MslI CAynn'nnrTG
Cuts at: 0 385 436 478 773 966 981
Size: 385 51 42 295 193 15
Fragments arranged by size:
385 295 193 51 42 15
MspA1I CmG'CkG
Cuts at: 0 30 155 981
Size: 30 125 826
Fragments arranged by size:
826 125 30
NspI r_CATG'y
Cuts at: 0 345 981
Size: 345 636
PasI CC'CwG_GG
Cuts at: 0 35 981
Size: 35 946
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 965 981
Size: 282 683 16
Fragments arranged by size:
683 282 16
PfoI T'CCnGG_A
Cuts at: 0 446 981
Size: 446 535
PmlI CAC'GTG
Cuts at: 0 552 981
Size: 552 429
PshAI GACnn'nnGTC
Cuts at: 0 595 981
Size: 595 386
PspOMI G'GGCC_C
Cuts at: 0 207 270 541 911 981
Size: 207 63 271 370 70
Fragments arranged by size:
370 271 207 70 63
PstI C_TGCA'G
Cuts at: 0 149 981
Size: 149 832
PvuII CAG'CTG
Cuts at: 0 30 155 981
Size: 30 125 826
Fragments arranged by size:
826 125 30
SacI G_AGCT'C
Cuts at: 0 323 569 981
Size: 323 246 412
Fragments arranged by size:
412 323 246
SexAI A'CCwGG_T
Cuts at: 0 395 981
Size: 395 586
SfcI C'TryA_G
Cuts at: 0 145 981
Size: 145 836
SmaI CCC'GGG
Cuts at: 0 175 981
Size: 175 806
SrfI GCCC'GGGC
Cuts at: 0 175 981
Size: 175 806
StuI AGG'CCT
Cuts at: 0 857 981
Size: 857 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 541 573 981
Size: 541 32 408
Fragments arranged by size:
541 408 32
XmaI C'CCGG_G
Cuts at: 0 173 981
Size: 173 808
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AloI ApaI
ApaLI AvaI BanI BanII BbsI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstYI
Bsu36I BtgZI BtsI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NspI
PasI PflMI PfoI PmlI PshAI PspOMI PstI PvuII
SacI SexAI SfcI SmaI SrfI StuI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AlwNI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmtI
BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmnI ZraI