Restriction map for
pF1KB5441
(Linear) (Six-base) MAPSORT of: KIBB5441.seq Check: 1640 from: 1 to: 1017
KIBB5441.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
Acc65I G'GTAC_C
Cuts at: 0 178 1017
Size: 178 839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 488 1017
Size: 253 235 529
Fragments arranged by size:
529 253 235
AflIII A'CryG_T
Cuts at: 0 804 1017
Size: 804 213
ApaI G_GGCC'C
Cuts at: 0 197 1017
Size: 197 820
ApoI r'AATT_y
Cuts at: 0 983 1017
Size: 983 34
BanI G'GyrC_C
Cuts at: 0 178 1017
Size: 178 839
BanII G_rGCy'C
Cuts at: 0 197 310 1017
Size: 197 113 707
Fragments arranged by size:
707 197 113
BciVI GTATCCnnnnn_n'
Cuts at: 0 670 1017
Size: 670 347
BglI GCCn_nnn'nGGC
Cuts at: 0 334 1017
Size: 334 683
BglII A'GATC_T
Cuts at: 0 626 725 1017
Size: 626 99 292
Fragments arranged by size:
626 292 99
Bme1580I G_kGCm'C
Cuts at: 0 134 197 1017
Size: 134 63 820
Fragments arranged by size:
820 134 63
BmgBI CAC'GTC
Cuts at: 0 384 570 807 1017
Size: 384 186 237 210
Fragments arranged by size:
384 237 210 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 918 1014 1017
Size: 918 96 3
Fragments arranged by size:
918 96 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 293 1017
Size: 293 724
BsaAI yAC'GTr
Cuts at: 0 119 1017
Size: 119 898
BsaBI GATnn'nnATC
Cuts at: 0 364 1017
Size: 364 653
BsaWI w'CCGG_w
Cuts at: 0 356 811 1017
Size: 356 455 206
Fragments arranged by size:
455 356 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 679 709 1017
Size: 292 30 357 30 308
Fragments arranged by size:
357 308 292 30 30
BseYI C'CCAG_C
Cuts at: 0 323 851 1017
Size: 323 528 166
Fragments arranged by size:
528 323 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1017
Size: 555 462
BsiHKAI G_wGCw'C
Cuts at: 0 566 1017
Size: 566 451
BsmBI CGTCTCn'nnnn_
Cuts at: 0 699 1017
Size: 699 318
Bsp1286I G_dGCh'C
Cuts at: 0 134 197 310 566 1017
Size: 134 63 113 256 451
Fragments arranged by size:
451 256 134 113 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 1017
Size: 419 598
BstYI r'GATC_y
Cuts at: 0 359 626 725 1017
Size: 359 267 99 292
Fragments arranged by size:
359 292 267 99
BtgI C'CryG_G
Cuts at: 0 743 1017
Size: 743 274
DraI TTT'AAA
Cuts at: 0 930 1017
Size: 930 87
EaeI y'GGCC_r
Cuts at: 0 41 268 719 828 1017
Size: 41 227 451 109 189
Fragments arranged by size:
451 227 189 109 41
EarI CTCTTCn'nnn_
Cuts at: 0 160 1017
Size: 160 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 1017
Size: 418 599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 488 1017
Size: 253 235 529
Fragments arranged by size:
529 253 235
EcoO109I rG'GnC_Cy
Cuts at: 0 59 228 408 1017
Size: 59 169 180 609
Fragments arranged by size:
609 180 169 59
EcoRI G'AATT_C
Cuts at: 0 983 1017
Size: 983 34
FspI TGC'GCA
Cuts at: 0 380 1017
Size: 380 637
FspAI rTGC'GCAy
Cuts at: 0 380 1017
Size: 380 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 1017
Size: 292 32 693
Fragments arranged by size:
693 292 32
HincII GTy'rAC
Cuts at: 0 93 102 573 1017
Size: 93 9 471 444
Fragments arranged by size:
471 444 93 9
HindIII A'AGCT_T
Cuts at: 0 246 470 1017
Size: 246 224 547
Fragments arranged by size:
547 246 224
KpnI G_GTAC'C
Cuts at: 0 182 1017
Size: 182 835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 1017
Size: 59 958
MscI TGG'CCA
Cuts at: 0 43 270 830 1017
Size: 43 227 560 187
Fragments arranged by size:
560 227 187 43
MslI CAynn'nnrTG
Cuts at: 0 374 1017
Size: 374 643
NspI r_CATG'y
Cuts at: 0 422 435 1017
Size: 422 13 582
Fragments arranged by size:
582 422 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 896 1017
Size: 896 121
PfoI T'CCnGG_A
Cuts at: 0 895 1017
Size: 895 122
PmlI CAC'GTG
Cuts at: 0 119 1017
Size: 119 898
PpuMI rG'GwC_Cy
Cuts at: 0 228 408 1017
Size: 228 180 609
Fragments arranged by size:
609 228 180
PspOMI G'GGCC_C
Cuts at: 0 193 1017
Size: 193 824
ScaI AGT'ACT
Cuts at: 0 1003 1017
Size: 1003 14
SfcI C'TryA_G
Cuts at: 0 642 687 1017
Size: 642 45 330
Fragments arranged by size:
642 330 45
StuI AGG'CCT
Cuts at: 0 497 1017
Size: 497 520
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 814 1017
Size: 178 636 203
Fragments arranged by size:
636 203 178
TatI w'GTAC_w
Cuts at: 0 1001 1017
Size: 1001 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 310 843 1017
Size: 310 533 174
Fragments arranged by size:
533 310 174
Enzymes that do cut:
Acc65I AcuI AflIII ApaI ApoI BanI BanII BciVI
BglI BglII Bme1580I BmgBI BmrI BsaI BsaAI BsaBI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BstYI BtgI DraI EaeI EarI EciI Eco57MI EcoO109I
EcoRI FspI FspAI Hin4I HincII HindIII KpnI MmeI
MscI MslI NspI PflMI PfoI PmlI PpuMI PspOMI
ScaI SfcI StuI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BlpI BmtI BplI BpmI Bpu10I BpuEI
BsaHI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FalI FseI HaeII
HpaI KasI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI