(Linear) (Six-base) MAPSORT of: KIBB5441.seq Check: 1640 from: 1 to: 1017 KIBB5441.orf With 182 enzymes: SgfI * February 28, 2007 11:27 .. Acc65I G'GTAC_C Cuts at: 0 178 1017 Size: 178 839 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 488 1017 Size: 253 235 529 Fragments arranged by size: 529 253 235 AflIII A'CryG_T Cuts at: 0 804 1017 Size: 804 213 ApaI G_GGCC'C Cuts at: 0 197 1017 Size: 197 820 ApoI r'AATT_y Cuts at: 0 983 1017 Size: 983 34 BanI G'GyrC_C Cuts at: 0 178 1017 Size: 178 839 BanII G_rGCy'C Cuts at: 0 197 310 1017 Size: 197 113 707 Fragments arranged by size: 707 197 113 BciVI GTATCCnnnnn_n' Cuts at: 0 670 1017 Size: 670 347 BglI GCCn_nnn'nGGC Cuts at: 0 334 1017 Size: 334 683 BglII A'GATC_T Cuts at: 0 626 725 1017 Size: 626 99 292 Fragments arranged by size: 626 292 99 Bme1580I G_kGCm'C Cuts at: 0 134 197 1017 Size: 134 63 820 Fragments arranged by size: 820 134 63 BmgBI CAC'GTC Cuts at: 0 384 570 807 1017 Size: 384 186 237 210 Fragments arranged by size: 384 237 210 186 BmrI ACTGGGnnnn_n' Cuts at: 0 918 1014 1017 Size: 918 96 3 Fragments arranged by size: 918 96 3 BsaI GGTCTCn'nnnn_ Cuts at: 0 293 1017 Size: 293 724 BsaAI yAC'GTr Cuts at: 0 119 1017 Size: 119 898 BsaBI GATnn'nnATC Cuts at: 0 364 1017 Size: 364 653 BsaWI w'CCGG_w Cuts at: 0 356 811 1017 Size: 356 455 206 Fragments arranged by size: 455 356 206 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 292 322 679 709 1017 Size: 292 30 357 30 308 Fragments arranged by size: 357 308 292 30 30 BseYI C'CCAG_C Cuts at: 0 323 851 1017 Size: 323 528 166 Fragments arranged by size: 528 323 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 555 1017 Size: 555 462 BsiHKAI G_wGCw'C Cuts at: 0 566 1017 Size: 566 451 BsmBI CGTCTCn'nnnn_ Cuts at: 0 699 1017 Size: 699 318 Bsp1286I G_dGCh'C Cuts at: 0 134 197 310 566 1017 Size: 134 63 113 256 451 Fragments arranged by size: 451 256 134 113 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 419 1017 Size: 419 598 BstYI r'GATC_y Cuts at: 0 359 626 725 1017 Size: 359 267 99 292 Fragments arranged by size: 359 292 267 99 BtgI C'CryG_G Cuts at: 0 743 1017 Size: 743 274 DraI TTT'AAA Cuts at: 0 930 1017 Size: 930 87 EaeI y'GGCC_r Cuts at: 0 41 268 719 828 1017 Size: 41 227 451 109 189 Fragments arranged by size: 451 227 189 109 41 EarI CTCTTCn'nnn_ Cuts at: 0 160 1017 Size: 160 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 418 1017 Size: 418 599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 488 1017 Size: 253 235 529 Fragments arranged by size: 529 253 235 EcoO109I rG'GnC_Cy Cuts at: 0 59 228 408 1017 Size: 59 169 180 609 Fragments arranged by size: 609 180 169 59 EcoRI G'AATT_C Cuts at: 0 983 1017 Size: 983 34 FspI TGC'GCA Cuts at: 0 380 1017 Size: 380 637 FspAI rTGC'GCAy Cuts at: 0 380 1017 Size: 380 637 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 292 324 1017 Size: 292 32 693 Fragments arranged by size: 693 292 32 HincII GTy'rAC Cuts at: 0 93 102 573 1017 Size: 93 9 471 444 Fragments arranged by size: 471 444 93 9 HindIII A'AGCT_T Cuts at: 0 246 470 1017 Size: 246 224 547 Fragments arranged by size: 547 246 224 KpnI G_GTAC'C Cuts at: 0 182 1017 Size: 182 835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 59 1017 Size: 59 958 MscI TGG'CCA Cuts at: 0 43 270 830 1017 Size: 43 227 560 187 Fragments arranged by size: 560 227 187 43 MslI CAynn'nnrTG Cuts at: 0 374 1017 Size: 374 643 NspI r_CATG'y Cuts at: 0 422 435 1017 Size: 422 13 582 Fragments arranged by size: 582 422 13 PflMI CCAn_nnn'nTGG Cuts at: 0 896 1017 Size: 896 121 PfoI T'CCnGG_A Cuts at: 0 895 1017 Size: 895 122 PmlI CAC'GTG Cuts at: 0 119 1017 Size: 119 898 PpuMI rG'GwC_Cy Cuts at: 0 228 408 1017 Size: 228 180 609 Fragments arranged by size: 609 228 180 PspOMI G'GGCC_C Cuts at: 0 193 1017 Size: 193 824 ScaI AGT'ACT Cuts at: 0 1003 1017 Size: 1003 14 SfcI C'TryA_G Cuts at: 0 642 687 1017 Size: 642 45 330 Fragments arranged by size: 642 330 45 StuI AGG'CCT Cuts at: 0 497 1017 Size: 497 520 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 178 814 1017 Size: 178 636 203 Fragments arranged by size: 636 203 178 TatI w'GTAC_w Cuts at: 0 1001 1017 Size: 1001 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 310 843 1017 Size: 310 533 174 Fragments arranged by size: 533 310 174 Enzymes that do cut: Acc65I AcuI AflIII ApaI ApoI BanI BanII BciVI BglI BglII Bme1580I BmgBI BmrI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstYI BtgI DraI EaeI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI Hin4I HincII HindIII KpnI MmeI MscI MslI NspI PflMI PfoI PmlI PpuMI PspOMI ScaI SfcI StuI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BlpI BmtI BplI BpmI Bpu10I BpuEI BsaHI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI EcoRV FalI FseI HaeII HpaI KasI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI