Restriction map for pF1KB5441
 (Linear) (Six-base) MAPSORT of: KIBB5441.seq  Check: 1640  from: 1  to: 1017



KIBB5441.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

Acc65I G'GTAC_C

Cuts at:      0     178    1017 
   Size:        178     839
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     488    1017 
   Size:        253     235     529

  Fragments arranged by size: 

                 529     253     235
 
AflIII A'CryG_T

Cuts at:      0     804    1017 
   Size:        804     213
 
ApaI G_GGCC'C

Cuts at:      0     197    1017 
   Size:        197     820
 
ApoI r'AATT_y

Cuts at:      0     983    1017 
   Size:        983      34
 
BanI G'GyrC_C

Cuts at:      0     178    1017 
   Size:        178     839
 
BanII G_rGCy'C

Cuts at:      0     197     310    1017 
   Size:        197     113     707

  Fragments arranged by size: 

                 707     197     113
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     670    1017 
   Size:        670     347
 
BglI GCCn_nnn'nGGC

Cuts at:      0     334    1017 
   Size:        334     683
 
BglII A'GATC_T

Cuts at:      0     626     725    1017 
   Size:        626      99     292

  Fragments arranged by size: 

                 626     292      99
 
Bme1580I G_kGCm'C

Cuts at:      0     134     197    1017 
   Size:        134      63     820

  Fragments arranged by size: 

                 820     134      63
 
BmgBI CAC'GTC

Cuts at:      0     384     570     807    1017 
   Size:        384     186     237     210

  Fragments arranged by size: 

                 384     237     210     186
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     918    1014    1017 
   Size:        918      96       3

  Fragments arranged by size: 

                 918      96       3
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     293    1017 
   Size:        293     724
 
BsaAI yAC'GTr

Cuts at:      0     119    1017 
   Size:        119     898
 
BsaBI GATnn'nnATC

Cuts at:      0     364    1017 
   Size:        364     653
 
BsaWI w'CCGG_w

Cuts at:      0     356     811    1017 
   Size:        356     455     206

  Fragments arranged by size: 

                 455     356     206
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     292     322     679     709    1017 
   Size:        292      30     357      30     308

  Fragments arranged by size: 

                 357     308     292      30      30
 
BseYI C'CCAG_C

Cuts at:      0     323     851    1017 
   Size:        323     528     166

  Fragments arranged by size: 

                 528     323     166
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    1017 
   Size:        555     462
 
BsiHKAI G_wGCw'C

Cuts at:      0     566    1017 
   Size:        566     451
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     699    1017 
   Size:        699     318
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     197     310     566    1017 
   Size:        134      63     113     256     451

  Fragments arranged by size: 

                 451     256     134     113      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     419    1017 
   Size:        419     598
 
BstYI r'GATC_y

Cuts at:      0     359     626     725    1017 
   Size:        359     267      99     292

  Fragments arranged by size: 

                 359     292     267      99
 
BtgI C'CryG_G

Cuts at:      0     743    1017 
   Size:        743     274
 
DraI TTT'AAA

Cuts at:      0     930    1017 
   Size:        930      87
 
EaeI y'GGCC_r

Cuts at:      0      41     268     719     828    1017 
   Size:         41     227     451     109     189

  Fragments arranged by size: 

                 451     227     189     109      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     160    1017 
   Size:        160     857
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     418    1017 
   Size:        418     599
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     488    1017 
   Size:        253     235     529

  Fragments arranged by size: 

                 529     253     235
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59     228     408    1017 
   Size:         59     169     180     609

  Fragments arranged by size: 

                 609     180     169      59
 
EcoRI G'AATT_C

Cuts at:      0     983    1017 
   Size:        983      34
 
FspI TGC'GCA

Cuts at:      0     380    1017 
   Size:        380     637
 
FspAI rTGC'GCAy

Cuts at:      0     380    1017 
   Size:        380     637
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324    1017 
   Size:        292      32     693

  Fragments arranged by size: 

                 693     292      32
 
HincII GTy'rAC

Cuts at:      0      93     102     573    1017 
   Size:         93       9     471     444

  Fragments arranged by size: 

                 471     444      93       9
 
HindIII A'AGCT_T

Cuts at:      0     246     470    1017 
   Size:        246     224     547

  Fragments arranged by size: 

                 547     246     224
 
KpnI G_GTAC'C

Cuts at:      0     182    1017 
   Size:        182     835
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59    1017 
   Size:         59     958
 
MscI TGG'CCA

Cuts at:      0      43     270     830    1017 
   Size:         43     227     560     187

  Fragments arranged by size: 

                 560     227     187      43
 
MslI CAynn'nnrTG

Cuts at:      0     374    1017 
   Size:        374     643
 
NspI r_CATG'y

Cuts at:      0     422     435    1017 
   Size:        422      13     582

  Fragments arranged by size: 

                 582     422      13
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     896    1017 
   Size:        896     121
 
PfoI T'CCnGG_A

Cuts at:      0     895    1017 
   Size:        895     122
 
PmlI CAC'GTG

Cuts at:      0     119    1017 
   Size:        119     898
 
PpuMI rG'GwC_Cy

Cuts at:      0     228     408    1017 
   Size:        228     180     609

  Fragments arranged by size: 

                 609     228     180
 
PspOMI G'GGCC_C

Cuts at:      0     193    1017 
   Size:        193     824
 
ScaI AGT'ACT

Cuts at:      0    1003    1017 
   Size:       1003      14
 
SfcI C'TryA_G

Cuts at:      0     642     687    1017 
   Size:        642      45     330

  Fragments arranged by size: 

                 642     330      45
 
StuI AGG'CCT

Cuts at:      0     497    1017 
   Size:        497     520
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     178     814    1017 
   Size:        178     636     203

  Fragments arranged by size: 

                 636     203     178
 
TatI w'GTAC_w

Cuts at:      0    1001    1017 
   Size:       1001      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     310     843    1017 
   Size:        310     533     174

  Fragments arranged by size: 

                 533     310     174
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     ApaI     ApoI     BanI    BanII    BciVI
     BglI    BglII Bme1580I    BmgBI     BmrI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BstYI     BtgI     DraI     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FspI    FspAI    Hin4I   HincII  HindIII     KpnI     MmeI
     MscI     MslI     NspI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     ScaI     SfcI     StuI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BlpI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaHI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI  EcoICRI    EcoNI    EcoRV     FalI     FseI    HaeII
     HpaI     KasI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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