Restriction map for
pF1KB5454
(Linear) (Six-base) MAPSORT of: KIBB5454.seq Check: 1978 from: 1 to: 1542
KIBB5454.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 1499 1542
Size: 1499 43
AccI GT'mk_AC
Cuts at: 0 892 1542
Size: 892 650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 364 1542
Size: 187 177 1178
Fragments arranged by size:
1178 187 177
AfeI AGC'GCT
Cuts at: 0 76 1542
Size: 76 1466
AhdI GACnn_n'nnGTC
Cuts at: 0 1005 1542
Size: 1005 537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 842 1369 1542
Size: 842 527 173
Fragments arranged by size:
842 527 173
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1542
Size: 1062 480
AvaI C'yCGr_G
Cuts at: 0 87 1542
Size: 87 1455
BanII G_rGCy'C
Cuts at: 0 919 1156 1542
Size: 919 237 386
Fragments arranged by size:
919 386 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 171 205 1542
Size: 171 34 1337
Fragments arranged by size:
1337 171 34
BglI GCCn_nnn'nGGC
Cuts at: 0 562 1542
Size: 562 980
BlpI GC'TnA_GC
Cuts at: 0 718 1542
Size: 718 824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 933 1194 1542
Size: 933 261 348
Fragments arranged by size:
933 348 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1342 1542
Size: 1156 186 200
Fragments arranged by size:
1156 200 186
Bpu10I CC'TnA_GC
Cuts at: 0 1155 1542
Size: 1155 387
BsaI GGTCTCn'nnnn_
Cuts at: 0 1182 1542
Size: 1182 360
BsaHI Gr'CG_yC
Cuts at: 0 1496 1542
Size: 1496 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1129 1159 1542
Size: 1129 30 383
Fragments arranged by size:
1129 383 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1060 1542
Size: 313 747 482
Fragments arranged by size:
747 482 313
BseYI C'CCAG_C
Cuts at: 0 45 565 1542
Size: 45 520 977
Fragments arranged by size:
977 520 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 820 1542
Size: 400 420 722
Fragments arranged by size:
722 420 400
BsiEI CG_ry'CG
Cuts at: 0 859 1542
Size: 859 683
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 1487 1542
Size: 55 1432 55
Fragments arranged by size:
1432 55 55
Bsp1286I G_dGCh'C
Cuts at: 0 919 1156 1542
Size: 919 237 386
Fragments arranged by size:
919 386 237
BspHI T'CATG_A
Cuts at: 0 683 1542
Size: 683 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 875 904 1542
Size: 875 29 638
Fragments arranged by size:
875 638 29
BsrBI CCG'CTC
Cuts at: 0 360 381 1542
Size: 360 21 1161
Fragments arranged by size:
1161 360 21
BsrDI GCAATG_nn'
Cuts at: 0 824 1542
Size: 824 718
BsrFI r'CCGG_y
Cuts at: 0 1211 1542
Size: 1211 331
BsrGI T'GTAC_A
Cuts at: 0 484 1542
Size: 484 1058
BstBI TT'CG_AA
Cuts at: 0 1218 1542
Size: 1218 324
BstEII G'GTnAC_C
Cuts at: 0 1530 1542
Size: 1530 12
BstYI r'GATC_y
Cuts at: 0 9 1542
Size: 9 1533
BtgI C'CryG_G
Cuts at: 0 557 921 936 1542
Size: 557 364 15 606
Fragments arranged by size:
606 557 364 15
BtsI GCAGTG_nn'
Cuts at: 0 768 1542
Size: 768 774
DraIII CAC_nnn'GTG
Cuts at: 0 1083 1542
Size: 1083 459
EaeI y'GGCC_r
Cuts at: 0 856 924 1542
Size: 856 68 618
Fragments arranged by size:
856 618 68
EagI C'GGCC_G
Cuts at: 0 856 1542
Size: 856 686
EarI CTCTTCn'nnn_
Cuts at: 0 1019 1542
Size: 1019 523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 364 1156 1342 1542
Size: 187 177 792 186 200
Fragments arranged by size:
792 200 187 186 177
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 1542
Size: 610 932
EcoO109I rG'GnC_Cy
Cuts at: 0 42 84 594 864 1542
Size: 42 42 510 270 678
Fragments arranged by size:
678 510 270 42 42
HaeII r_GCGC'y
Cuts at: 0 78 1542
Size: 78 1464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 766 798 991 1023 1542
Size: 217 32 517 32 193 32 519
Fragments arranged by size:
519 517 217 193 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1126 1542
Size: 1126 416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 350 1542
Size: 342 8 1192
Fragments arranged by size:
1192 342 8
MslI CAynn'nnrTG
Cuts at: 0 842 926 1351 1542
Size: 842 84 425 191
Fragments arranged by size:
842 425 191 84
MspA1I CmG'CkG
Cuts at: 0 105 1542
Size: 105 1437
NaeI GCC'GGC
Cuts at: 0 1213 1542
Size: 1213 329
NcoI C'CATG_G
Cuts at: 0 921 1542
Size: 921 621
NdeI CA'TA_TG
Cuts at: 0 1271 1542
Size: 1271 271
NgoMIV G'CCGG_C
Cuts at: 0 1211 1542
Size: 1211 331
PasI CC'CwG_GG
Cuts at: 0 1062 1542
Size: 1062 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 1062 1542
Size: 1062 480
PfoI T'CCnGG_A
Cuts at: 0 605 1319 1542
Size: 605 714 223
Fragments arranged by size:
714 605 223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 1542
Size: 602 32 908
Fragments arranged by size:
908 602 32
PpuMI rG'GwC_Cy
Cuts at: 0 42 84 594 864 1542
Size: 42 42 510 270 678
Fragments arranged by size:
678 510 270 42 42
PsiI TTA'TAA
Cuts at: 0 743 1542
Size: 743 799
PstI C_TGCA'G
Cuts at: 0 276 1520 1542
Size: 276 1244 22
Fragments arranged by size:
1244 276 22
PvuII CAG'CTG
Cuts at: 0 105 1542
Size: 105 1437
SanDI GG'GwC_CC
Cuts at: 0 42 1542
Size: 42 1500
ScaI AGT'ACT
Cuts at: 0 502 1542
Size: 502 1040
SexAI A'CCwGG_T
Cuts at: 0 515 1542
Size: 515 1027
SfcI C'TryA_G
Cuts at: 0 272 1516 1542
Size: 272 1244 26
Fragments arranged by size:
1244 272 26
SmaI CCC'GGG
Cuts at: 0 89 1542
Size: 89 1453
SpeI A'CTAG_T
Cuts at: 0 93 1542
Size: 93 1449
SspI AAT'ATT
Cuts at: 0 1089 1542
Size: 1089 453
StuI AGG'CCT
Cuts at: 0 1141 1542
Size: 1141 401
StyI C'CwwG_G
Cuts at: 0 641 921 1542
Size: 641 280 621
Fragments arranged by size:
641 621 280
TatI w'GTAC_w
Cuts at: 0 484 500 983 1542
Size: 484 16 483 559
Fragments arranged by size:
559 484 483 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 462 494 1542
Size: 462 32 1048
Fragments arranged by size:
1048 462 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1245 1542
Size: 1245 297
XmaI C'CCGG_G
Cuts at: 0 87 1542
Size: 87 1455
XmnI GAAnn'nnTTC
Cuts at: 0 76 1372 1542
Size: 76 1296 170
Fragments arranged by size:
1296 170 76
ZraI GAC'GTC
Cuts at: 0 1497 1542
Size: 1497 45
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AlfI AlwNI AvaI
BanII BcgI BglI BlpI BplI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII
BstYI BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII MmeI MslI MspA1I
NaeI NcoI NdeI NgoMIV PasI PflMI PfoI PpiI
PpuMI PsiI PstI PvuII SanDI ScaI SexAI SfcI
SmaI SpeI SspI StuI StyI TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BpuEI BsaAI BsaBI
BsaWI BsiHKAI BsiWI BsmI BspEI BssHII BssSI BstAPI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MscI NarI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SphI SrfI SwaI TaqII Tth111I XbaI
XhoI