Restriction map for
pF1KB5455
(Linear) (Six-base) MAPSORT of: KIBB5455.seq Check: 8628 from: 1 to: 1125
KIBB5455.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 29 1125
Size: 29 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1125
Size: 220 905
AvaI C'yCGr_G
Cuts at: 0 80 292 1125
Size: 80 212 833
Fragments arranged by size:
833 212 80
BanI G'GyrC_C
Cuts at: 0 107 116 257 904 960 1125
Size: 107 9 141 647 56 165
Fragments arranged by size:
647 165 141 107 56 9
BbeI G_GCGC'C
Cuts at: 0 111 1125
Size: 111 1014
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 1125
Size: 81 1044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 521 1125
Size: 487 34 604
Fragments arranged by size:
604 487 34
BglI GCCn_nnn'nGGC
Cuts at: 0 82 1125
Size: 82 1043
Bme1580I G_kGCm'C
Cuts at: 0 491 1125
Size: 491 634
BmgBI CAC'GTC
Cuts at: 0 858 1125
Size: 858 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 1125
Size: 243 882
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 208 1125
Size: 208 917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1125
Size: 727 398
Bpu10I CC'TnA_GC
Cuts at: 0 998 1125
Size: 998 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 1125
Size: 367 758
BsaBI GATnn'nnATC
Cuts at: 0 982 1066 1125
Size: 982 84 59
Fragments arranged by size:
982 84 59
BsaHI Gr'CG_yC
Cuts at: 0 108 1125
Size: 108 1017
BsaWI w'CCGG_w
Cuts at: 0 463 1125
Size: 463 662
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 631 661 1125
Size: 22 30 579 30 464
Fragments arranged by size:
579 464 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 690 983 1125
Size: 310 380 293 142
Fragments arranged by size:
380 310 293 142
BseYI C'CCAG_C
Cuts at: 0 387 964 1125
Size: 387 577 161
Fragments arranged by size:
577 387 161
BsiEI CG_ry'CG
Cuts at: 0 611 1125
Size: 611 514
BsiHKAI G_wGCw'C
Cuts at: 0 218 302 1125
Size: 218 84 823
Fragments arranged by size:
823 218 84
Bsp1286I G_dGCh'C
Cuts at: 0 218 302 491 1125
Size: 218 84 189 634
Fragments arranged by size:
634 218 189 84
BspHI T'CATG_A
Cuts at: 0 566 845 1125
Size: 566 279 280
Fragments arranged by size:
566 280 279
BsrBI CCG'CTC
Cuts at: 0 615 759 1125
Size: 615 144 366
Fragments arranged by size:
615 366 144
BsrDI GCAATG_nn'
Cuts at: 0 759 923 986 1125
Size: 759 164 63 139
Fragments arranged by size:
759 164 139 63
BsrFI r'CCGG_y
Cuts at: 0 55 1125
Size: 55 1070
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 1125
Size: 306 819
BstEII G'GTnAC_C
Cuts at: 0 474 1125
Size: 474 651
BstYI r'GATC_y
Cuts at: 0 572 1125
Size: 572 553
Bsu36I CC'TnA_GG
Cuts at: 0 773 1125
Size: 773 352
BtgI C'CryG_G
Cuts at: 0 605 683 1125
Size: 605 78 442
Fragments arranged by size:
605 442 78
BtsI GCAGTG_nn'
Cuts at: 0 950 1125
Size: 950 175
DraIII CAC_nnn'GTG
Cuts at: 0 306 486 1125
Size: 306 180 639
Fragments arranged by size:
639 306 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 736 1125
Size: 736 389
EaeI y'GGCC_r
Cuts at: 0 435 543 608 680 732 1125
Size: 435 108 65 72 52 393
Fragments arranged by size:
435 393 108 72 65 52
EagI C'GGCC_G
Cuts at: 0 608 1125
Size: 608 517
EarI CTCTTCn'nnn_
Cuts at: 0 706 787 1125
Size: 706 81 338
Fragments arranged by size:
706 338 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 727 1125
Size: 220 507 398
Fragments arranged by size:
507 398 220
EcoO109I rG'GnC_Cy
Cuts at: 0 321 549 1125
Size: 321 228 576
Fragments arranged by size:
576 321 228
EcoRV GAT'ATC
Cuts at: 0 12 1125
Size: 12 1113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 1125
Size: 75 1050
HaeII r_GCGC'y
Cuts at: 0 111 1125
Size: 111 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 975 1007 1125
Size: 22 32 921 32 118
Fragments arranged by size:
921 118 32 32 22
HincII GTy'rAC
Cuts at: 0 30 1125
Size: 30 1095
KasI G'GCGC_C
Cuts at: 0 107 1125
Size: 107 1018
MscI TGG'CCA
Cuts at: 0 682 1125
Size: 682 443
MslI CAynn'nnrTG
Cuts at: 0 454 910 1125
Size: 454 456 215
Fragments arranged by size:
456 454 215
MspA1I CmG'CkG
Cuts at: 0 767 1125
Size: 767 358
NaeI GCC'GGC
Cuts at: 0 57 1125
Size: 57 1068
NarI GG'CG_CC
Cuts at: 0 108 1125
Size: 108 1017
NgoMIV G'CCGG_C
Cuts at: 0 55 1125
Size: 55 1070
NspI r_CATG'y
Cuts at: 0 48 1125
Size: 48 1077
PpuMI rG'GwC_Cy
Cuts at: 0 549 1125
Size: 549 576
SalI G'TCGA_C
Cuts at: 0 28 1125
Size: 28 1097
SapI GCTCTTCn'nnn_
Cuts at: 0 706 1125
Size: 706 419
ScaI AGT'ACT
Cuts at: 0 1009 1125
Size: 1009 116
SfcI C'TryA_G
Cuts at: 0 591 1125
Size: 591 534
SfoI GGC'GCC
Cuts at: 0 109 1125
Size: 109 1016
SmaI CCC'GGG
Cuts at: 0 82 1125
Size: 82 1043
StyI C'CwwG_G
Cuts at: 0 335 1125
Size: 335 790
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 493 978 1125
Size: 331 162 485 147
Fragments arranged by size:
485 331 162 147
TatI w'GTAC_w
Cuts at: 0 1007 1125
Size: 1007 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 631 663 1125
Size: 631 32 462
Fragments arranged by size:
631 462 32
Tth111I GACn'n_nGTC
Cuts at: 0 472 1125
Size: 472 653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 702 1125
Size: 528 174 423
Fragments arranged by size:
528 423 174
XmaI C'CCGG_G
Cuts at: 0 80 1125
Size: 80 1045
Enzymes that do cut:
AccI AcuI AvaI BanI BbeI BbsI BcgI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BsrBI BsrDI BsrFI BstAPI BstEII BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EagI EarI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PpuMI SalI
SapI ScaI SfcI SfoI SmaI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaAI
BsgI BsiWI BsmI BsmBI BspEI BspMI BsrGI BssHII
BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SanDI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI