Restriction map for
pF1KB5463
(Linear) (Six-base) MAPSORT of: KIBB5463.seq Check: 1231 from: 1 to: 864
KIBB5463.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 864
Size: 96 768
AccI GT'mk_AC
Cuts at: 0 722 864
Size: 722 142
AfeI AGC'GCT
Cuts at: 0 430 864
Size: 430 434
AflIII A'CryG_T
Cuts at: 0 641 864
Size: 641 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 864
Size: 310 32 522
Fragments arranged by size:
522 310 32
AlwNI CAG_nnn'CTG
Cuts at: 0 573 864
Size: 573 291
AscI GG'CGCG_CC
Cuts at: 0 781 864
Size: 781 83
BamHI G'GATC_C
Cuts at: 0 341 864
Size: 341 523
BanII G_rGCy'C
Cuts at: 0 23 864
Size: 23 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 256 355 864
Size: 214 42 99 509
Fragments arranged by size:
509 214 99 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 816 850 864
Size: 816 34 14
Fragments arranged by size:
816 34 14
BclI T'GATC_A
Cuts at: 0 800 864
Size: 800 64
BglI GCCn_nnn'nGGC
Cuts at: 0 184 864
Size: 184 680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 514 526 537 634 864
Size: 478 36 12 11 97 230
Fragments arranged by size:
478 230 97 36 12 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 71 864
Size: 71 793
BsaBI GATnn'nnATC
Cuts at: 0 805 864
Size: 805 59
BsaWI w'CCGG_w
Cuts at: 0 344 864
Size: 344 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 376 406 864
Size: 310 30 36 30 458
Fragments arranged by size:
458 310 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 169 232 315 502 864
Size: 127 42 63 83 187 362
Fragments arranged by size:
362 187 127 83 63 42
BsiHKAI G_wGCw'C
Cuts at: 0 23 864
Size: 23 841
BsmBI CGTCTCn'nnnn_
Cuts at: 0 580 864
Size: 580 284
Bsp1286I G_dGCh'C
Cuts at: 0 23 864
Size: 23 841
BspEI T'CCGG_A
Cuts at: 0 344 864
Size: 344 520
BspHI T'CATG_A
Cuts at: 0 797 864
Size: 797 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 96 864
Size: 96 768
BsrBI CCG'CTC
Cuts at: 0 323 864
Size: 323 541
BsrFI r'CCGG_y
Cuts at: 0 466 864
Size: 466 398
BssHII G'CGCG_C
Cuts at: 0 781 864
Size: 781 83
BssSI C'ACGA_G
Cuts at: 0 664 864
Size: 664 200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 666 864
Size: 666 198
BstYI r'GATC_y
Cuts at: 0 341 864
Size: 341 523
Bsu36I CC'TnA_GG
Cuts at: 0 337 864
Size: 337 527
BtgI C'CryG_G
Cuts at: 0 68 864
Size: 68 796
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 56 819 864
Size: 56 763 45
Fragments arranged by size:
763 56 45
EaeI y'GGCC_r
Cuts at: 0 29 656 864
Size: 29 627 208
Fragments arranged by size:
627 208 29
EcoICRI GAG'CTC
Cuts at: 0 21 864
Size: 21 843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 514 526 537 634 864
Size: 478 36 12 11 97 230
Fragments arranged by size:
478 230 97 36 12 11
HaeII r_GCGC'y
Cuts at: 0 432 864
Size: 432 432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 864
Size: 376 32 456
Fragments arranged by size:
456 376 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 374 440 864
Size: 263 111 66 424
Fragments arranged by size:
424 263 111 66
MscI TGG'CCA
Cuts at: 0 658 864
Size: 658 206
MslI CAynn'nnrTG
Cuts at: 0 646 652 658 664 864
Size: 646 6 6 6 200
Fragments arranged by size:
646 200 6 6 6
MspA1I CmG'CkG
Cuts at: 0 331 456 864
Size: 331 125 408
Fragments arranged by size:
408 331 125
NspI r_CATG'y
Cuts at: 0 504 645 864
Size: 504 141 219
Fragments arranged by size:
504 219 141
PciI A'CATG_T
Cuts at: 0 641 864
Size: 641 223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 864
Size: 310 32 522
Fragments arranged by size:
522 310 32
PvuII CAG'CTG
Cuts at: 0 456 864
Size: 456 408
SacI G_AGCT'C
Cuts at: 0 23 864
Size: 23 841
StuI AGG'CCT
Cuts at: 0 314 864
Size: 314 550
StyI C'CwwG_G
Cuts at: 0 32 864
Size: 32 832
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 23 89 864
Size: 23 66 775
Fragments arranged by size:
775 66 23
TatI w'GTAC_w
Cuts at: 0 398 701 864
Size: 398 303 163
Fragments arranged by size:
398 303 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 159 191 864
Size: 159 32 673
Fragments arranged by size:
673 159 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 489 864
Size: 378 111 375
Fragments arranged by size:
378 375 111
Enzymes that do cut:
AarI AccI AfeI AflIII AloI AlwNI AscI BamHI
BanII BbsI BcgI BclI BglI BpmI BsaI BsaBI
BsaWI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI Bsu36I
BtgI BtgZI EaeI EcoICRI Eco57MI HaeII Hin4I MmeI
MscI MslI MspA1I NspI PciI PpiI PvuII SacI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaHI BseYI BsgI BsiEI BsiWI BsmI BsrDI BsrGI
BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI