Restriction map for
pF1KB5464
(Linear) (Six-base) MAPSORT of: KIBB5464.seq Check: 3038 from: 1 to: 798
KIBB5464.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 798
Size: 187 611
Acc65I G'GTAC_C
Cuts at: 0 132 798
Size: 132 666
AflIII A'CryG_T
Cuts at: 0 254 798
Size: 254 544
ApoI r'AATT_y
Cuts at: 0 382 769 798
Size: 382 387 29
Fragments arranged by size:
387 382 29
BanI G'GyrC_C
Cuts at: 0 132 798
Size: 132 666
BanII G_rGCy'C
Cuts at: 0 59 308 798
Size: 59 249 490
Fragments arranged by size:
490 249 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 760 798
Size: 726 34 38
Fragments arranged by size:
726 38 34
BclI T'GATC_A
Cuts at: 0 636 798
Size: 636 162
BglII A'GATC_T
Cuts at: 0 471 798
Size: 471 327
BmgBI CAC'GTC
Cuts at: 0 255 798
Size: 255 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 798
Size: 562 236
BsaBI GATnn'nnATC
Cuts at: 0 419 798
Size: 419 379
BsaHI Gr'CG_yC
Cuts at: 0 95 798
Size: 95 703
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 227 798
Size: 85 142 571
Fragments arranged by size:
571 142 85
BseYI C'CCAG_C
Cuts at: 0 321 798
Size: 321 477
BsiHKAI G_wGCw'C
Cuts at: 0 605 798
Size: 605 193
Bsp1286I G_dGCh'C
Cuts at: 0 59 308 605 798
Size: 59 249 297 193
Fragments arranged by size:
297 249 193 59
BspHI T'CATG_A
Cuts at: 0 438 597 669 798
Size: 438 159 72 129
Fragments arranged by size:
438 159 129 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 798
Size: 187 611
BsrBI CCG'CTC
Cuts at: 0 25 65 798
Size: 25 40 733
Fragments arranged by size:
733 40 25
BsrGI T'GTAC_A
Cuts at: 0 224 798
Size: 224 574
BstYI r'GATC_y
Cuts at: 0 471 798
Size: 471 327
BtgI C'CryG_G
Cuts at: 0 776 798
Size: 776 22
EaeI y'GGCC_r
Cuts at: 0 141 798
Size: 141 657
EarI CTCTTCn'nnn_
Cuts at: 0 144 798
Size: 144 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 798
Size: 562 236
EcoO109I rG'GnC_Cy
Cuts at: 0 117 798
Size: 117 681
HaeII r_GCGC'y
Cuts at: 0 79 798
Size: 79 719
KpnI G_GTAC'C
Cuts at: 0 136 798
Size: 136 662
MfeI C'AATT_G
Cuts at: 0 341 738 798
Size: 341 397 60
Fragments arranged by size:
397 341 60
MscI TGG'CCA
Cuts at: 0 143 798
Size: 143 655
MslI CAynn'nnrTG
Cuts at: 0 520 798
Size: 520 278
MspA1I CmG'CkG
Cuts at: 0 325 798
Size: 325 473
NspI r_CATG'y
Cuts at: 0 171 798
Size: 171 627
PpuMI rG'GwC_Cy
Cuts at: 0 117 798
Size: 117 681
PstI C_TGCA'G
Cuts at: 0 315 798
Size: 315 483
PvuII CAG'CTG
Cuts at: 0 325 798
Size: 325 473
SapI GCTCTTCn'nnn_
Cuts at: 0 144 798
Size: 144 654
SfcI C'TryA_G
Cuts at: 0 311 798
Size: 311 487
SspI AAT'ATT
Cuts at: 0 232 354 798
Size: 232 122 444
Fragments arranged by size:
444 232 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 543 798
Size: 543 255
TatI w'GTAC_w
Cuts at: 0 224 257 275 798
Size: 224 33 18 523
Fragments arranged by size:
523 224 33 18
Tth111I GACn'n_nGTC
Cuts at: 0 97 665 798
Size: 97 568 133
Fragments arranged by size:
568 133 97
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 798
Size: 537 261
Enzymes that do cut:
AarI Acc65I AflIII ApoI BanI BanII BcgI BclI
BglII BmgBI BpmI BsaBI BsaHI BseRI BseYI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrGI BstYI BtgI EaeI
EarI Eco57MI EcoO109I HaeII KpnI MfeI MscI MslI
MspA1I NspI PpuMI PstI PvuII SapI SfcI SspI
TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TstI XbaI XhoI XmaI XmnI ZraI