(Linear) (Six-base) MAPSORT of: KIBB5469.seq  Check: 8592  from: 1  to: 474



KIBB5469.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0     125     474 
   Size:        125     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     446     474 
   Size:        446      28
 
BsmI GAATG_Cn'

Cuts at:      0     203     474 
   Size:        203     271
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     412     474 
   Size:        412      62
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     334     474 
   Size:        334     140
 
BsrDI GCAATG_nn'

Cuts at:      0      62     474 
   Size:         62     412
 
BstYI r'GATC_y

Cuts at:      0     387     474 
   Size:        387      87
 
BtsI GCAGTG_nn'

Cuts at:      0     439     474 
   Size:        439      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     474 
   Size:        187     287
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     474 
   Size:         19     455
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     345     377     474 
   Size:        345      32      97

  Fragments arranged by size: 

                 345      97      32
 
MslI CAynn'nnrTG

Cuts at:      0     208     474 
   Size:        208     266
 
PstI C_TGCA'G

Cuts at:      0     451     474 
   Size:        451      23
 
SfcI C'TryA_G

Cuts at:      0     447     474 
   Size:        447      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      75     474 
   Size:         75     399
 

 Enzymes that do cut:

     AccI    AlwNI     BsmI    BsmBI    BspMI    BsrDI    BstYI     BtsI
     EarI     EciI    Hin4I     MslI     PstI     SfcI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI