Restriction map for
pF1KB5486
(Linear) (Six-base) MAPSORT of: KIBB5486.seq Check: 7523 from: 1 to: 579
KIBB5486.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 441 579
Size: 297 144 138
Fragments arranged by size:
297 144 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 579
Size: 376 203
AlwNI CAG_nnn'CTG
Cuts at: 0 45 579
Size: 45 534
ApoI r'AATT_y
Cuts at: 0 502 579
Size: 502 77
AvaI C'yCGr_G
Cuts at: 0 28 579
Size: 28 551
BanI G'GyrC_C
Cuts at: 0 232 514 579
Size: 232 282 65
Fragments arranged by size:
282 232 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 579
Size: 195 384
BglI GCCn_nnn'nGGC
Cuts at: 0 119 579
Size: 119 460
BglII A'GATC_T
Cuts at: 0 149 452 579
Size: 149 303 127
Fragments arranged by size:
303 149 127
BlpI GC'TnA_GC
Cuts at: 0 265 579
Size: 265 314
BmgBI CAC'GTC
Cuts at: 0 201 579
Size: 201 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 385 579
Size: 310 75 194
Fragments arranged by size:
310 194 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 286 579
Size: 88 198 293
Fragments arranged by size:
293 198 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 579
Size: 409 30 140
Fragments arranged by size:
409 140 30
BseYI C'CCAG_C
Cuts at: 0 559 579
Size: 559 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 579
Size: 394 185
BsiHKAI G_wGCw'C
Cuts at: 0 200 579
Size: 200 379
Bsp1286I G_dGCh'C
Cuts at: 0 200 579
Size: 200 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 579
Size: 53 526
BsrDI GCAATG_nn'
Cuts at: 0 257 579
Size: 257 322
BsrFI r'CCGG_y
Cuts at: 0 118 579
Size: 118 461
BssSI C'ACGA_G
Cuts at: 0 73 579
Size: 73 506
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 579
Size: 45 534
BstYI r'GATC_y
Cuts at: 0 149 452 579
Size: 149 303 127
Fragments arranged by size:
303 149 127
BtgI C'CryG_G
Cuts at: 0 131 579
Size: 131 448
BtsI GCAGTG_nn'
Cuts at: 0 556 570 579
Size: 556 14 9
Fragments arranged by size:
556 14 9
EaeI y'GGCC_r
Cuts at: 0 120 579
Size: 120 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 529 579
Size: 529 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 310 385 441 579
Size: 297 13 75 56 138
Fragments arranged by size:
297 138 75 56 13
EcoO109I rG'GnC_Cy
Cuts at: 0 42 579
Size: 42 537
EcoRI G'AATT_C
Cuts at: 0 502 579
Size: 502 77
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 327 579
Size: 327 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 579
Size: 409 32 138
Fragments arranged by size:
409 138 32
NspI r_CATG'y
Cuts at: 0 321 579
Size: 321 258
PfoI T'CCnGG_A
Cuts at: 0 245 579
Size: 245 334
PpuMI rG'GwC_Cy
Cuts at: 0 42 579
Size: 42 537
PstI C_TGCA'G
Cuts at: 0 41 83 551 579
Size: 41 42 468 28
Fragments arranged by size:
468 42 41 28
SfcI C'TryA_G
Cuts at: 0 37 79 547 579
Size: 37 42 468 32
Fragments arranged by size:
468 42 37 32
SmlI C'TyrA_G
Cuts at: 0 103 301 579
Size: 103 198 278
Fragments arranged by size:
278 198 103
StyI C'CwwG_G
Cuts at: 0 494 579
Size: 494 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 579
Size: 387 192
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AvaI BanI BbsI BglI
BglII BlpI BmgBI BpmI BpuEI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstYI
BtgI BtsI EaeI EciI Eco57MI EcoO109I EcoRI FalI
Hin4I NspI PfoI PpuMI PstI SfcI SmlI StyI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI Bme1580I BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI