Restriction map for
pF1KB5487
(Linear) (Six-base) MAPSORT of: KIBB5487.seq Check: 4986 from: 1 to: 744
KIBB5487.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 569 744
Size: 569 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 709 744
Size: 232 477 35
Fragments arranged by size:
477 232 35
AflIII A'CryG_T
Cuts at: 0 17 744
Size: 17 727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 448 744
Size: 448 296
AlwNI CAG_nnn'CTG
Cuts at: 0 337 687 744
Size: 337 350 57
Fragments arranged by size:
350 337 57
ApaLI G'TGCA_C
Cuts at: 0 21 744
Size: 21 723
AvaI C'yCGr_G
Cuts at: 0 631 744
Size: 631 113
BanI G'GyrC_C
Cuts at: 0 79 118 424 744
Size: 79 39 306 320
Fragments arranged by size:
320 306 79 39
BbeI G_GCGC'C
Cuts at: 0 83 122 744
Size: 83 39 622
Fragments arranged by size:
622 83 39
BbvCI CC'TCA_GC
Cuts at: 0 491 744
Size: 491 253
Bme1580I G_kGCm'C
Cuts at: 0 25 427 744
Size: 25 402 317
Fragments arranged by size:
402 317 25
BmgBI CAC'GTC
Cuts at: 0 585 744
Size: 585 159
BmtI G_CTAG'C
Cuts at: 0 511 744
Size: 511 233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 744
Size: 248 496
Bpu10I CC'TnA_GC
Cuts at: 0 292 491 744
Size: 292 199 253
Fragments arranged by size:
292 253 199
BsaAI yAC'GTr
Cuts at: 0 405 744
Size: 405 339
BsaHI Gr'CG_yC
Cuts at: 0 80 119 744
Size: 80 39 625
Fragments arranged by size:
625 80 39
BsaWI w'CCGG_w
Cuts at: 0 152 744
Size: 152 592
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 708 744
Size: 708 36
BseYI C'CCAG_C
Cuts at: 0 326 744
Size: 326 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 744
Size: 427 317
BsiEI CG_ry'CG
Cuts at: 0 90 744
Size: 90 654
BsiHKAI G_wGCw'C
Cuts at: 0 25 744
Size: 25 719
BsmI GAATG_Cn'
Cuts at: 0 320 744
Size: 320 424
Bsp1286I G_dGCh'C
Cuts at: 0 25 427 744
Size: 25 402 317
Fragments arranged by size:
402 317 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 744
Size: 187 557
BsrFI r'CCGG_y
Cuts at: 0 85 744
Size: 85 659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 744
Size: 129 615
BtgI C'CryG_G
Cuts at: 0 56 110 744
Size: 56 54 634
Fragments arranged by size:
634 56 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 12 744
Size: 12 732
BtsI GCAGTG_nn'
Cuts at: 0 306 744
Size: 306 438
EaeI y'GGCC_r
Cuts at: 0 87 449 744
Size: 87 362 295
Fragments arranged by size:
362 295 87
EagI C'GGCC_G
Cuts at: 0 87 744
Size: 87 657
EarI CTCTTCn'nnn_
Cuts at: 0 251 685 744
Size: 251 434 59
Fragments arranged by size:
434 251 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 248 709 744
Size: 232 16 461 35
Fragments arranged by size:
461 232 35 16
EcoO109I rG'GnC_Cy
Cuts at: 0 684 744
Size: 684 60
HaeII r_GCGC'y
Cuts at: 0 83 122 578 744
Size: 83 39 456 166
Fragments arranged by size:
456 166 83 39
HincII GTy'rAC
Cuts at: 0 486 744
Size: 486 258
KasI G'GCGC_C
Cuts at: 0 79 118 744
Size: 79 39 626
Fragments arranged by size:
626 79 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 744
Size: 89 655
MscI TGG'CCA
Cuts at: 0 451 744
Size: 451 293
MspA1I CmG'CkG
Cuts at: 0 58 125 309 744
Size: 58 67 184 435
Fragments arranged by size:
435 184 67 58
NaeI GCC'GGC
Cuts at: 0 87 744
Size: 87 657
NarI GG'CG_CC
Cuts at: 0 80 119 744
Size: 80 39 625
Fragments arranged by size:
625 80 39
NgoMIV G'CCGG_C
Cuts at: 0 85 744
Size: 85 659
NheI G'CTAG_C
Cuts at: 0 507 744
Size: 507 237
NspI r_CATG'y
Cuts at: 0 21 519 681 744
Size: 21 498 162 63
Fragments arranged by size:
498 162 63 21
PciI A'CATG_T
Cuts at: 0 17 744
Size: 17 727
PmlI CAC'GTG
Cuts at: 0 405 744
Size: 405 339
PpuMI rG'GwC_Cy
Cuts at: 0 684 744
Size: 684 60
PsiI TTA'TAA
Cuts at: 0 560 744
Size: 560 184
PstI C_TGCA'G
Cuts at: 0 301 506 744
Size: 301 205 238
Fragments arranged by size:
301 238 205
PvuII CAG'CTG
Cuts at: 0 309 744
Size: 309 435
SacII CC_GC'GG
Cuts at: 0 59 744
Size: 59 685
SapI GCTCTTCn'nnn_
Cuts at: 0 251 744
Size: 251 493
SfcI C'TryA_G
Cuts at: 0 297 502 570 744
Size: 297 205 68 174
Fragments arranged by size:
297 205 174 68
SfoI GGC'GCC
Cuts at: 0 81 120 744
Size: 81 39 624
Fragments arranged by size:
624 81 39
SphI G_CATG'C
Cuts at: 0 519 744
Size: 519 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 687 744
Size: 687 57
TatI w'GTAC_w
Cuts at: 0 625 744
Size: 625 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 744
Size: 134 32 578
Fragments arranged by size:
578 134 32
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaLI AvaI BanI
BbeI BbvCI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BstXI BtgI BtgZI BtsI EaeI
EagI EarI Eco57MI EcoO109I HaeII HincII KasI MmeI
MscI MspA1I NaeI NarI NgoMIV NheI NspI PciI
PmlI PpuMI PsiI PstI PvuII SacII SapI SfcI
SfoI SphI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BplI BpuEI BsaI BsaBI
BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI MslI NcoI NdeI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI