Restriction map for
pF1KB5490
(Linear) (Six-base) MAPSORT of: KIBB5490.seq Check: 1090 from: 1 to: 1482
KIBB5490.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 629 1482
Size: 337 292 853
Fragments arranged by size:
853 337 292
AflIII A'CryG_T
Cuts at: 0 55 1253 1482
Size: 55 1198 229
Fragments arranged by size:
1198 229 55
AhdI GACnn_n'nnGTC
Cuts at: 0 1170 1327 1482
Size: 1170 157 155
Fragments arranged by size:
1170 157 155
AlwNI CAG_nnn'CTG
Cuts at: 0 468 582 736 979 1482
Size: 468 114 154 243 503
Fragments arranged by size:
503 468 243 154 114
ApaLI G'TGCA_C
Cuts at: 0 1082 1482
Size: 1082 400
ApoI r'AATT_y
Cuts at: 0 135 456 846 873 1409 1433 1482
Size: 135 321 390 27 536 24 49
Fragments arranged by size:
536 390 321 135 49 27 24
AvrII C'CTAG_G
Cuts at: 0 1114 1482
Size: 1114 368
BamHI G'GATC_C
Cuts at: 0 1197 1482
Size: 1197 285
BanI G'GyrC_C
Cuts at: 0 1219 1482
Size: 1219 263
BanII G_rGCy'C
Cuts at: 0 1343 1482
Size: 1343 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 1391 1441 1482
Size: 635 756 50 41
Fragments arranged by size:
756 635 50 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 427 1482
Size: 427 1055
BglII A'GATC_T
Cuts at: 0 17 312 1482
Size: 17 295 1170
Fragments arranged by size:
1170 295 17
Bme1580I G_kGCm'C
Cuts at: 0 1086 1224 1482
Size: 1086 138 258
Fragments arranged by size:
1086 258 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 1482
Size: 612 870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1482
Size: 539 943
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 725 755 1482
Size: 725 30 727
Fragments arranged by size:
727 725 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 945 948 1482
Size: 945 3 534
Fragments arranged by size:
945 534 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 595 1482
Size: 53 542 887
Fragments arranged by size:
887 542 53
BsiHKAI G_wGCw'C
Cuts at: 0 602 717 1086 1482
Size: 602 115 369 396
Fragments arranged by size:
602 396 369 115
BsmI GAATG_Cn'
Cuts at: 0 1009 1280 1482
Size: 1009 271 202
Fragments arranged by size:
1009 271 202
Bsp1286I G_dGCh'C
Cuts at: 0 602 717 1086 1224 1343 1482
Size: 602 115 369 138 119 139
Fragments arranged by size:
602 369 139 138 119 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 894 1482
Size: 894 588
BsrDI GCAATG_nn'
Cuts at: 0 65 1482
Size: 65 1417
BssSI C'ACGA_G
Cuts at: 0 1180 1482
Size: 1180 302
BstAPI GCAn_nnn'nTGC
Cuts at: 0 670 1482
Size: 670 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 781 1482
Size: 781 701
BstYI r'GATC_y
Cuts at: 0 17 312 620 1134 1197 1455 1482
Size: 17 295 308 514 63 258 27
Fragments arranged by size:
514 308 295 258 63 27 17
DraIII CAC_nnn'GTG
Cuts at: 0 1247 1482
Size: 1247 235
EarI CTCTTCn'nnn_
Cuts at: 0 740 1482
Size: 740 742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 539 629 1482
Size: 337 202 90 853
Fragments arranged by size:
853 337 202 90
EcoO109I rG'GnC_Cy
Cuts at: 0 49 510 579 1482
Size: 49 461 69 903
Fragments arranged by size:
903 461 69 49
EcoRI G'AATT_C
Cuts at: 0 135 1409 1482
Size: 135 1274 73
Fragments arranged by size:
1274 135 73
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 265 1058 1323 1482
Size: 265 793 265 159
Fragments arranged by size:
793 265 265 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 605 637 1156 1188 1313 1345 1482
Size: 605 32 519 32 125 32 137
Fragments arranged by size:
605 519 137 125 32 32 32
HindIII A'AGCT_T
Cuts at: 0 329 1482
Size: 329 1153
MfeI C'AATT_G
Cuts at: 0 1382 1482
Size: 1382 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 298 497 1482
Size: 24 274 199 985
Fragments arranged by size:
985 274 199 24
MslI CAynn'nnrTG
Cuts at: 0 913 1097 1148 1482
Size: 913 184 51 334
Fragments arranged by size:
913 334 184 51
NdeI CA'TA_TG
Cuts at: 0 74 1203 1416 1482
Size: 74 1129 213 66
Fragments arranged by size:
1129 213 74 66
NspI r_CATG'y
Cuts at: 0 59 1214 1257 1482
Size: 59 1155 43 225
Fragments arranged by size:
1155 225 59 43
PciI A'CATG_T
Cuts at: 0 55 1253 1482
Size: 55 1198 229
Fragments arranged by size:
1198 229 55
PfoI T'CCnGG_A
Cuts at: 0 946 1331 1482
Size: 946 385 151
Fragments arranged by size:
946 385 151
PpuMI rG'GwC_Cy
Cuts at: 0 49 510 1482
Size: 49 461 972
Fragments arranged by size:
972 461 49
ScaI AGT'ACT
Cuts at: 0 533 1482
Size: 533 949
SspI AAT'ATT
Cuts at: 0 222 1066 1482
Size: 222 844 416
Fragments arranged by size:
844 416 222
StyI C'CwwG_G
Cuts at: 0 1114 1193 1482
Size: 1114 79 289
Fragments arranged by size:
1114 289 79
TatI w'GTAC_w
Cuts at: 0 531 1482
Size: 531 951
XmnI GAAnn'nnTTC
Cuts at: 0 1280 1482
Size: 1280 202
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaLI ApoI AvrII BamHI
BanI BanII BbsI BciVI BglII Bme1580I BmrI BpmI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BssSI BstAPI BstXI BstYI DraIII EarI Eco57MI EcoO109I
EcoRI FalI Hin4I HindIII MfeI MmeI MslI NdeI
NspI PciI PfoI PpuMI ScaI SspI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
BaeI BbeI BbvCI BcgI BclI BfrBI BglI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI MscI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI