Restriction map for
pF1KB5497
(Linear) (Six-base) MAPSORT of: KIBB5497.seq Check: 4112 from: 1 to: 1437
KIBB5497.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1362 1437
Size: 67 1295 75
Fragments arranged by size:
1295 75 67
AlwNI CAG_nnn'CTG
Cuts at: 0 270 297 1437
Size: 270 27 1140
Fragments arranged by size:
1140 270 27
ApoI r'AATT_y
Cuts at: 0 992 1038 1076 1244 1437
Size: 992 46 38 168 193
Fragments arranged by size:
992 193 168 46 38
AvaI C'yCGr_G
Cuts at: 0 194 244 326 1437
Size: 194 50 82 1111
Fragments arranged by size:
1111 194 82 50
BanII G_rGCy'C
Cuts at: 0 224 320 649 1097 1437
Size: 224 96 329 448 340
Fragments arranged by size:
448 340 329 224 96
BciVI GTATCCnnnnn_n'
Cuts at: 0 1033 1437
Size: 1033 404
BclI T'GATC_A
Cuts at: 0 299 1437
Size: 299 1138
BlpI GC'TnA_GC
Cuts at: 0 1316 1437
Size: 1316 121
Bme1580I G_kGCm'C
Cuts at: 0 24 1437
Size: 24 1413
BmgBI CAC'GTC
Cuts at: 0 363 1437
Size: 363 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 304 307 397 1022 1274 1437
Size: 292 12 3 90 625 252 163
Fragments arranged by size:
625 292 252 163 90 12 3
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 241 1437
Size: 175 66 1196
Fragments arranged by size:
1196 175 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 1437
Size: 133 30 1274
Fragments arranged by size:
1274 133 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 556 894 1105 1132 1437
Size: 472 84 338 211 27 305
Fragments arranged by size:
472 338 305 211 84 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1437
Size: 352 1085
BsiHKAI G_wGCw'C
Cuts at: 0 224 320 1437
Size: 224 96 1117
Fragments arranged by size:
1117 224 96
BsmI GAATG_Cn'
Cuts at: 0 972 1437
Size: 972 465
Bsp1286I G_dGCh'C
Cuts at: 0 24 224 320 649 1097 1437
Size: 24 200 96 329 448 340
Fragments arranged by size:
448 340 329 200 96 24
BspHI T'CATG_A
Cuts at: 0 855 1437
Size: 855 582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 483 1437
Size: 483 954
BsrDI GCAATG_nn'
Cuts at: 0 945 1437
Size: 945 492
BstYI r'GATC_y
Cuts at: 0 266 393 1437
Size: 266 127 1044
Fragments arranged by size:
1044 266 127
Bsu36I CC'TnA_GG
Cuts at: 0 311 1437
Size: 311 1126
BtgI C'CryG_G
Cuts at: 0 626 1437
Size: 626 811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 927 962 1095 1130 1437
Size: 927 35 133 35 307
Fragments arranged by size:
927 307 133 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 173 588 1437
Size: 173 415 849
Fragments arranged by size:
849 415 173
EcoICRI GAG'CTC
Cuts at: 0 222 318 1437
Size: 222 96 1119
Fragments arranged by size:
1119 222 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 292 304 307 397 1022 1274 1362
Size: 67 225 12 3 90 625 252 88
Cuts at: 1362 1437
Size: 75
Fragments arranged by size:
625 252 225 90 88 75 67 12
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 310 473 876 1437
Size: 136 174 163 403 561
Fragments arranged by size:
561 403 174 163 136
EcoO109I rG'GnC_Cy
Cuts at: 0 190 1437
Size: 190 1247
EcoRI G'AATT_C
Cuts at: 0 992 1076 1244 1437
Size: 992 84 168 193
Fragments arranged by size:
992 193 168 84
EcoRV GAT'ATC
Cuts at: 0 1414 1437
Size: 1414 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 70 95 102 453 485 789 821
Size: 63 7 25 7 351 32 304 32
Cuts at: 821 1437
Size: 616
Fragments arranged by size:
616 351 304 63 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 229 1437
Size: 229 1208
HindIII A'AGCT_T
Cuts at: 0 565 1205 1437
Size: 565 640 232
Fragments arranged by size:
640 565 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1320 1437
Size: 1320 117
MslI CAynn'nnrTG
Cuts at: 0 506 860 917 1437
Size: 506 354 57 520
Fragments arranged by size:
520 506 354 57
MspA1I CmG'CkG
Cuts at: 0 390 1437
Size: 390 1047
NcoI C'CATG_G
Cuts at: 0 626 1437
Size: 626 811
NdeI CA'TA_TG
Cuts at: 0 1279 1437
Size: 1279 158
NspI r_CATG'y
Cuts at: 0 751 1324 1437
Size: 751 573 113
Fragments arranged by size:
751 573 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 732 1437
Size: 30 702 705
Fragments arranged by size:
705 702 30
PfoI T'CCnGG_A
Cuts at: 0 215 269 1437
Size: 215 54 1168
Fragments arranged by size:
1168 215 54
PpuMI rG'GwC_Cy
Cuts at: 0 190 1437
Size: 190 1247
PvuII CAG'CTG
Cuts at: 0 390 1437
Size: 390 1047
SacI G_AGCT'C
Cuts at: 0 224 320 1437
Size: 224 96 1117
Fragments arranged by size:
1117 224 96
SfcI C'TryA_G
Cuts at: 0 238 1437
Size: 238 1199
SmaI CCC'GGG
Cuts at: 0 328 1437
Size: 328 1109
SmlI C'TyrA_G
Cuts at: 0 194 1437
Size: 194 1243
StuI AGG'CCT
Cuts at: 0 131 974 1135 1437
Size: 131 843 161 302
Fragments arranged by size:
843 302 161 131
StyI C'CwwG_G
Cuts at: 0 132 257 626 1437
Size: 132 125 369 811
Fragments arranged by size:
811 369 132 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 359 1162 1437
Size: 359 803 275
Fragments arranged by size:
803 359 275
TatI w'GTAC_w
Cuts at: 0 480 754 1437
Size: 480 274 683
Fragments arranged by size:
683 480 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 401 433 1437
Size: 133 32 236 32 1004
Fragments arranged by size:
1004 236 133 32 32
XbaI T'CTAG_A
Cuts at: 0 832 1437
Size: 832 605
XhoI C'TCGA_G
Cuts at: 0 194 1437
Size: 194 1243
XmaI C'CCGG_G
Cuts at: 0 326 1437
Size: 326 1111
XmnI GAAnn'nnTTC
Cuts at: 0 1291 1437
Size: 1291 146
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanII BciVI BclI BlpI
Bme1580I BmgBI BpmI BsaI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BstYI Bsu36I BtgI
CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MmeI MslI MspA1I NcoI NdeI
NspI PflMI PfoI PpuMI PvuII SacI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EciI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XcmI ZraI