Restriction map for
pF1KB5499
(Linear) (Six-base) MAPSORT of: KIBB5499.seq Check: 3919 from: 1 to: 4827
KIBB5499.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 302 1695 4827
Size: 302 1393 3132
Fragments arranged by size:
3132 1393 302
AccI GT'mk_AC
Cuts at: 0 941 4827
Size: 941 3886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1622 1857 2569 2951 2962 4067 4217
Size: 1334 288 235 712 382 11 1105 150
Cuts at: 4217 4827
Size: 610
Fragments arranged by size:
1334 1105 712 610 382 288 235 150
11
AfeI AGC'GCT
Cuts at: 0 145 2899 4302 4827
Size: 145 2754 1403 525
Fragments arranged by size:
2754 1403 525 145
AflII C'TTAA_G
Cuts at: 0 1803 4827
Size: 1803 3024
AflIII A'CryG_T
Cuts at: 0 200 202 4827
Size: 200 2 4625
Fragments arranged by size:
4625 200 2
AgeI A'CCGG_T
Cuts at: 0 2585 4827
Size: 2585 2242
AleI CACnn'nnGTG
Cuts at: 0 439 2979 4827
Size: 439 2540 1848
Fragments arranged by size:
2540 1848 439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2655 4724 4827
Size: 2655 2069 103
Fragments arranged by size:
2655 2069 103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 541 573 4827
Size: 541 32 4254
Fragments arranged by size:
4254 541 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3735 4233 4339 4827
Size: 3735 498 106 488
Fragments arranged by size:
3735 498 488 106
ApaI G_GGCC'C
Cuts at: 0 512 4827
Size: 512 4315
ApaLI G'TGCA_C
Cuts at: 0 117 2373 4827
Size: 117 2256 2454
Fragments arranged by size:
2454 2256 117
ApoI r'AATT_y
Cuts at: 0 622 895 1927 3291 4827
Size: 622 273 1032 1364 1536
Fragments arranged by size:
1536 1364 1032 622 273
AseI AT'TA_AT
Cuts at: 0 4550 4827
Size: 4550 277
AvaI C'yCGr_G
Cuts at: 0 38 154 217 1466 1612 3752 4019 4827
Size: 38 116 63 1249 146 2140 267 808
Fragments arranged by size:
2140 1249 808 267 146 116 63 38
BanI G'GyrC_C
Cuts at: 0 1126 1142 2293 2392 4618 4827
Size: 1126 16 1151 99 2226 209
Fragments arranged by size:
2226 1151 1126 209 99 16
BanII G_rGCy'C
Cuts at: 0 512 1213 2417 2824 3826 4827
Size: 512 701 1204 407 1002 1001
Fragments arranged by size:
1204 1002 1001 701 512 407
BbeI G_GCGC'C
Cuts at: 0 1130 4827
Size: 1130 3697
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 949 1784 1863 4786 4827
Size: 509 440 835 79 2923 41
Fragments arranged by size:
2923 835 509 440 79 41
BbvCI CC'TCA_GC
Cuts at: 0 4540 4827
Size: 4540 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4486 4520 4827
Size: 4486 34 307
Fragments arranged by size:
4486 307 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4065 4575 4827
Size: 4065 510 252
Fragments arranged by size:
4065 510 252
BclI T'GATC_A
Cuts at: 0 3129 4827
Size: 3129 1698
BglI GCCn_nnn'nGGC
Cuts at: 0 25 42 70 85 1091 2873 3775 3811
Size: 25 17 28 15 1006 1782 902 36
Cuts at: 3811 4228 4261 4282 4827
Size: 417 33 21 545
Fragments arranged by size:
1782 1006 902 545 417 36 33 28
25 21 17 15
BglII A'GATC_T
Cuts at: 0 3797 4365 4827
Size: 3797 568 462
Fragments arranged by size:
3797 568 462
BlpI GC'TnA_GC
Cuts at: 0 3815 3820 4827
Size: 3815 5 1007
Fragments arranged by size:
3815 1007 5
Bme1580I G_kGCm'C
Cuts at: 0 99 121 512 2377 3408 4827
Size: 99 22 391 1865 1031 1419
Fragments arranged by size:
1865 1419 1031 391 99 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 656 3191 4827
Size: 656 2535 1636
Fragments arranged by size:
2535 1636 656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1638 1928 3160 4827
Size: 1638 290 1232 1667
Fragments arranged by size:
1667 1638 1232 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2060 2348 3130 3865 3994 4702 4786 4827
Size: 2060 288 782 735 129 708 84 41
Fragments arranged by size:
2060 782 735 708 288 129 84 41
Bpu10I CC'TnA_GC
Cuts at: 0 1829 4540 4827
Size: 1829 2711 287
Fragments arranged by size:
2711 1829 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 793 1810 3475 4472 4827
Size: 433 360 1017 1665 997 355
Fragments arranged by size:
1665 1017 997 433 360 355
BsaI GGTCTCn'nnnn_
Cuts at: 0 1846 2185 2432 4827
Size: 1846 339 247 2395
Fragments arranged by size:
2395 1846 339 247
BsaAI yAC'GTr
Cuts at: 0 203 1969 2730 4827
Size: 203 1766 761 2097
Fragments arranged by size:
2097 1766 761 203
BsaBI GATnn'nnATC
Cuts at: 0 3436 4827
Size: 3436 1391
BsaHI Gr'CG_yC
Cuts at: 0 278 1127 4827
Size: 278 849 3700
Fragments arranged by size:
3700 849 278
BsaWI w'CCGG_w
Cuts at: 0 2585 3359 4827
Size: 2585 774 1468
Fragments arranged by size:
2585 1468 774
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 541 571 770 800 2356 2386
Size: 376 30 135 30 199 30 1556 30
Cuts at: 2386 4827
Size: 2441
Fragments arranged by size:
2441 1556 376 199 135 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 999 1536 2289 3778 4168 4528 4753 4827
Size: 999 537 753 1489 390 360 225 74
Fragments arranged by size:
1489 999 753 537 390 360 225 74
BseYI C'CCAG_C
Cuts at: 0 388 685 4223 4569 4827
Size: 388 297 3538 346 258
Fragments arranged by size:
3538 388 346 297 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 394 400 2052 2280 2508 4827
Size: 253 141 6 1652 228 228 2319
Fragments arranged by size:
2319 1652 253 228 228 141 6
BsiEI CG_ry'CG
Cuts at: 0 80 139 979 4148 4827
Size: 80 59 840 3169 679
Fragments arranged by size:
3169 840 679 80 59
BsiHKAI G_wGCw'C
Cuts at: 0 121 2028 2377 2417 4827
Size: 121 1907 349 40 2410
Fragments arranged by size:
2410 1907 349 121 40
BsmI GAATG_Cn'
Cuts at: 0 1018 1054 1497 2548 3010 4320 4827
Size: 1018 36 443 1051 462 1310 507
Fragments arranged by size:
1310 1051 1018 507 462 443 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 451 4827
Size: 451 4376
Bsp1286I G_dGCh'C
Cuts at: 0 99 121 512 1213 2028 2377 2417 2824
Size: 99 22 391 701 815 349 40 407
Cuts at: 2824 3408 3826 4827
Size: 584 418 1001
Fragments arranged by size:
1001 815 701 584 418 407 391 349
99 40 22
BspEI T'CCGG_A
Cuts at: 0 3359 4827
Size: 3359 1468
BspHI T'CATG_A
Cuts at: 0 4745 4827
Size: 4745 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 1695 3647 4827
Size: 302 1393 1952 1180
Fragments arranged by size:
1952 1393 1180 302
BsrBI CCG'CTC
Cuts at: 0 3426 4827
Size: 3426 1401
BsrDI GCAATG_nn'
Cuts at: 0 1038 1676 2184 4827
Size: 1038 638 508 2643
Fragments arranged by size:
2643 1038 638 508
BsrFI r'CCGG_y
Cuts at: 0 24 974 2585 2851 3104 3778 4827
Size: 24 950 1611 266 253 674 1049
Fragments arranged by size:
1611 1049 950 674 266 253 24
BsrGI T'GTAC_A
Cuts at: 0 1510 4827
Size: 1510 3317
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1331 1481 2648 4827
Size: 1331 150 1167 2179
Fragments arranged by size:
2179 1331 1167 150
BstEII G'GTnAC_C
Cuts at: 0 243 252 4827
Size: 243 9 4575
Fragments arranged by size:
4575 243 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2746 4827
Size: 2746 2081
BstYI r'GATC_y
Cuts at: 0 2965 3732 3797 4365 4827
Size: 2965 767 65 568 462
Fragments arranged by size:
2965 767 568 462 65
Bsu36I CC'TnA_GG
Cuts at: 0 2960 4827
Size: 2960 1867
BtgI C'CryG_G
Cuts at: 0 83 107 570 979 1086 4827
Size: 83 24 463 409 107 3741
Fragments arranged by size:
3741 463 409 107 83 24
BtsI GCAGTG_nn'
Cuts at: 0 2268 2496 2564 2716 2842 4245 4827
Size: 2268 228 68 152 126 1403 582
Fragments arranged by size:
2268 1403 582 228 152 126 68
ClaI AT'CG_AT
Cuts at: 0 2501 4827
Size: 2501 2326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 186 221 1950 1985 2039 2074 2348 2383
Size: 186 35 1729 35 54 35 274 35
Cuts at: 2383 4827
Size: 2444
Fragments arranged by size:
2444 1729 274 186 54 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 350 1226 1228 4827
Size: 350 876 2 3599
Fragments arranged by size:
3599 876 350 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 2153 4827
Size: 2153 2674
EaeI y'GGCC_r
Cuts at: 0 62 77 136 191 371 976 1083 2736
Size: 62 15 59 55 180 605 107 1653
Cuts at: 2736 2853 4145 4827
Size: 117 1292 682
Fragments arranged by size:
1653 1292 682 605 180 117 107 62
59 55 15
EagI C'GGCC_G
Cuts at: 0 77 136 976 4145 4827
Size: 77 59 840 3169 682
Fragments arranged by size:
3169 840 682 77 59
EarI CTCTTCn'nnn_
Cuts at: 0 1191 2121 3368 3536 3683 4827
Size: 1191 930 1247 168 147 1144
Fragments arranged by size:
1247 1191 1144 930 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 1150 4264 4300 4827
Size: 203 947 3114 36 527
Fragments arranged by size:
3114 947 527 203 36
EcoICRI GAG'CTC
Cuts at: 0 2415 4827
Size: 2415 2412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1622 1857 2060 2348 2569 2951 2962
Size: 1334 288 235 203 288 221 382 11
Cuts at: 2962 3130 3865 3994 4067 4217 4702 4786 4827
Size: 168 735 129 73 150 485 84 41
Fragments arranged by size:
1334 735 485 382 288 288 235 221
203 168 150 129 84 73 41 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1626 2207 3259 4827
Size: 1626 581 1052 1568
Fragments arranged by size:
1626 1568 1052 581
EcoO109I rG'GnC_Cy
Cuts at: 0 509 3513 3924 4038 4230 4827
Size: 509 3004 411 114 192 597
Fragments arranged by size:
3004 597 509 411 192 114
EcoRI G'AATT_C
Cuts at: 0 622 4827
Size: 622 4205
EcoRV GAT'ATC
Cuts at: 0 1638 4734 4827
Size: 1638 3096 93
Fragments arranged by size:
3096 1638 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1887 4827
Size: 1887 2940
HaeII r_GCGC'y
Cuts at: 0 147 692 1130 2901 4304 4827
Size: 147 545 438 1771 1403 523
Fragments arranged by size:
1771 1403 545 523 438 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1704 1736 2184 2216 2399 2431 4827
Size: 1704 32 448 32 183 32 2396
Fragments arranged by size:
2396 1704 448 183 32 32 32
HincII GTy'rAC
Cuts at: 0 165 2910 3285 3623 4827
Size: 165 2745 375 338 1204
Fragments arranged by size:
2745 1204 375 338 165
HindIII A'AGCT_T
Cuts at: 0 422 3553 4827
Size: 422 3131 1274
Fragments arranged by size:
3131 1274 422
KasI G'GCGC_C
Cuts at: 0 1126 4827
Size: 1126 3701
MfeI C'AATT_G
Cuts at: 0 1416 2652 4827
Size: 1416 1236 2175
Fragments arranged by size:
2175 1416 1236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2496 4827
Size: 2496 2331
MscI TGG'CCA
Cuts at: 0 193 1085 2738 4827
Size: 193 892 1653 2089
Fragments arranged by size:
2089 1653 892 193
MslI CAynn'nnrTG
Cuts at: 0 439 2979 3868 4459 4827
Size: 439 2540 889 591 368
Fragments arranged by size:
2540 889 591 439 368
MspA1I CmG'CkG
Cuts at: 0 85 572 1250 2690 3819 4569 4578 4827
Size: 85 487 678 1440 1129 750 9 249
Fragments arranged by size:
1440 1129 750 678 487 249 85 9
NaeI GCC'GGC
Cuts at: 0 26 4827
Size: 26 4801
NarI GG'CG_CC
Cuts at: 0 1127 4827
Size: 1127 3700
NcoI C'CATG_G
Cuts at: 0 107 1086 4827
Size: 107 979 3741
Fragments arranged by size:
3741 979 107
NdeI CA'TA_TG
Cuts at: 0 933 4827
Size: 933 3894
NgoMIV G'CCGG_C
Cuts at: 0 24 4827
Size: 24 4803
NotI GC'GGCC_GC
Cuts at: 0 77 136 4827
Size: 77 59 4691
Fragments arranged by size:
4691 77 59
NspI r_CATG'y
Cuts at: 0 153 4827
Size: 153 4674
PasI CC'CwG_GG
Cuts at: 0 2445 4233 4827
Size: 2445 1788 594
Fragments arranged by size:
2445 1788 594
PflMI CCAn_nnn'nTGG
Cuts at: 0 350 2817 4827
Size: 350 2467 2010
Fragments arranged by size:
2467 2010 350
PfoI T'CCnGG_A
Cuts at: 0 2325 3146 4827
Size: 2325 821 1681
Fragments arranged by size:
2325 1681 821
PmlI CAC'GTG
Cuts at: 0 203 2730 4827
Size: 203 2527 2097
Fragments arranged by size:
2527 2097 203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 4827
Size: 541 32 4254
Fragments arranged by size:
4254 541 32
PpuMI rG'GwC_Cy
Cuts at: 0 3513 3924 4827
Size: 3513 411 903
Fragments arranged by size:
3513 903 411
PshAI GACnn'nnGTC
Cuts at: 0 250 4827
Size: 250 4577
PspOMI G'GGCC_C
Cuts at: 0 508 4827
Size: 508 4319
PstI C_TGCA'G
Cuts at: 0 299 722 2227 2292 2780 4406 4827
Size: 299 423 1505 65 488 1626 421
Fragments arranged by size:
1626 1505 488 423 421 299 65
PvuII CAG'CTG
Cuts at: 0 1250 2690 3819 4569 4578 4827
Size: 1250 1440 1129 750 9 249
Fragments arranged by size:
1440 1250 1129 750 249 9
SacI G_AGCT'C
Cuts at: 0 2417 4827
Size: 2417 2410
SacII CC_GC'GG
Cuts at: 0 86 573 4827
Size: 86 487 4254
Fragments arranged by size:
4254 487 86
SanDI GG'GwC_CC
Cuts at: 0 3513 4827
Size: 3513 1314
SapI GCTCTTCn'nnn_
Cuts at: 0 1191 3683 4827
Size: 1191 2492 1144
Fragments arranged by size:
2492 1191 1144
ScaI AGT'ACT
Cuts at: 0 529 4827
Size: 529 4298
SexAI A'CCwGG_T
Cuts at: 0 346 4827
Size: 346 4481
SfcI C'TryA_G
Cuts at: 0 295 531 718 842 1197 1527 2223 2288
Size: 295 236 187 124 355 330 696 65
Cuts at: 2288 2776 3307 3506 4402 4827
Size: 488 531 199 896 425
Fragments arranged by size:
896 696 531 488 425 355 330 295
236 199 187 124 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 1091 3775 4827
Size: 25 1066 2684 1052
Fragments arranged by size:
2684 1066 1052 25
SfoI GGC'GCC
Cuts at: 0 1128 4827
Size: 1128 3699
SmaI CCC'GGG
Cuts at: 0 40 4827
Size: 40 4787
SmlI C'TyrA_G
Cuts at: 0 448 808 1612 1789 1803 3454 4487 4827
Size: 448 360 804 177 14 1651 1033 340
Fragments arranged by size:
1651 1033 804 448 360 340 177 14
SphI G_CATG'C
Cuts at: 0 153 4827
Size: 153 4674
StuI AGG'CCT
Cuts at: 0 607 1908 4827
Size: 607 1301 2919
Fragments arranged by size:
2919 1301 607
StyI C'CwwG_G
Cuts at: 0 107 560 1086 1164 1900 2816 3174 4034
Size: 107 453 526 78 736 916 358 860
Cuts at: 4034 4328 4827
Size: 294 499
Fragments arranged by size:
916 860 736 526 499 453 358 294
107 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 1562 2046 3056 4827
Size: 334 1228 484 1010 1771
Fragments arranged by size:
1771 1228 1010 484 334
TatI w'GTAC_w
Cuts at: 0 527 613 1510 3756 4827
Size: 527 86 897 2246 1071
Fragments arranged by size:
2246 1071 897 527 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 1965 1997 2356 2367 2388 2399
Size: 770 32 1163 32 359 11 21 11
Cuts at: 2399 4827
Size: 2428
Fragments arranged by size:
2428 1163 770 359 32 32 21 11
11
Tth111I GACn'n_nGTC
Cuts at: 0 1402 4827
Size: 1402 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 532 2920 4827
Size: 532 2388 1907
Fragments arranged by size:
2388 1907 532
XhoI C'TCGA_G
Cuts at: 0 1612 4827
Size: 1612 3215
XmaI C'CCGG_G
Cuts at: 0 38 4827
Size: 38 4789
XmnI GAAnn'nnTTC
Cuts at: 0 1156 4827
Size: 1156 3671
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SapI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AhdI AscI AsiSI AvrII BaeI
BamHI BfrBI BmgBI BmtI BsiWI BssHII BssSI BstBI
BstZ17I BtgZI DraI FseI FspI FspAI HpaI KpnI
MluI NheI NruI NsiI PacI PciI PmeI PsiI
PspXI PsrI PvuI RsrII SalI SbfI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI ZraI