Restriction map for
pF1KB5503
(Linear) (Six-base) MAPSORT of: KIBB5503.seq Check: 4300 from: 1 to: 1665
KIBB5503.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 728 1665
Size: 728 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 675 1665
Size: 154 521 990
Fragments arranged by size:
990 521 154
AfeI AGC'GCT
Cuts at: 0 642 1665
Size: 642 1023
AhdI GACnn_n'nnGTC
Cuts at: 0 714 1665
Size: 714 951
AleI CACnn'nnGTG
Cuts at: 0 199 256 1665
Size: 199 57 1409
Fragments arranged by size:
1409 199 57
AlwNI CAG_nnn'CTG
Cuts at: 0 1344 1665
Size: 1344 321
ApaI G_GGCC'C
Cuts at: 0 491 1665
Size: 491 1174
AvaI C'yCGr_G
Cuts at: 0 41 1665
Size: 41 1624
BamHI G'GATC_C
Cuts at: 0 934 1665
Size: 934 731
BanI G'GyrC_C
Cuts at: 0 186 787 1445 1665
Size: 186 601 658 220
Fragments arranged by size:
658 601 220 186
BanII G_rGCy'C
Cuts at: 0 421 491 1287 1665
Size: 421 70 796 378
Fragments arranged by size:
796 421 378 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 1663 1665
Size: 115 1548 2
Fragments arranged by size:
1548 115 2
BglI GCCn_nnn'nGGC
Cuts at: 0 13 1665
Size: 13 1652
BglII A'GATC_T
Cuts at: 0 1616 1665
Size: 1616 49
BlpI GC'TnA_GC
Cuts at: 0 1199 1665
Size: 1199 466
Bme1580I G_kGCm'C
Cuts at: 0 224 491 1448 1595 1665
Size: 224 267 957 147 70
Fragments arranged by size:
957 267 224 147 70
BmgBI CAC'GTC
Cuts at: 0 1380 1665
Size: 1380 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 741 910 1665
Size: 741 169 755
Fragments arranged by size:
755 741 169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 674 1665
Size: 674 991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 1016 1645 1662 1665
Size: 855 161 629 17 3
Fragments arranged by size:
855 629 161 17 3
Bpu10I CC'TnA_GC
Cuts at: 0 986 1665
Size: 986 679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1665
Size: 646 1019
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 1621 1665
Size: 112 1509 44
Fragments arranged by size:
1509 112 44
BsaAI yAC'GTr
Cuts at: 0 435 981 1665
Size: 435 546 684
Fragments arranged by size:
684 546 435
BsaHI Gr'CG_yC
Cuts at: 0 1438 1665
Size: 1438 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 340 370 1665
Size: 340 30 1295
Fragments arranged by size:
1295 340 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 361 441 1510 1665
Size: 358 3 80 1069 155
Fragments arranged by size:
1069 358 155 80 3
BseYI C'CCAG_C
Cuts at: 0 87 930 1064 1205 1665
Size: 87 843 134 141 460
Fragments arranged by size:
843 460 141 134 87
BsiEI CG_ry'CG
Cuts at: 0 8 1665
Size: 8 1657
BsiHKAI G_wGCw'C
Cuts at: 0 965 1665
Size: 965 700
BsmI GAATG_Cn'
Cuts at: 0 168 783 1665
Size: 168 615 882
Fragments arranged by size:
882 615 168
Bsp1286I G_dGCh'C
Cuts at: 0 224 421 491 965 1287 1448 1595 1665
Size: 224 197 70 474 322 161 147 70
Fragments arranged by size:
474 322 224 197 161 147 70 70
BsrBI CCG'CTC
Cuts at: 0 99 1665
Size: 99 1566
BsrDI GCAATG_nn'
Cuts at: 0 839 1665
Size: 839 826
BsrFI r'CCGG_y
Cuts at: 0 1271 1665
Size: 1271 394
BsrGI T'GTAC_A
Cuts at: 0 1007 1394 1665
Size: 1007 387 271
Fragments arranged by size:
1007 387 271
BssHII G'CGCG_C
Cuts at: 0 51 1665
Size: 51 1614
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 154 1665
Size: 87 67 1511
Fragments arranged by size:
1511 87 67
BstYI r'GATC_y
Cuts at: 0 934 1616 1665
Size: 934 682 49
Fragments arranged by size:
934 682 49
Bsu36I CC'TnA_GG
Cuts at: 0 401 1665
Size: 401 1264
BtgI C'CryG_G
Cuts at: 0 113 1665
Size: 113 1552
BtsI GCAGTG_nn'
Cuts at: 0 372 509 1665
Size: 372 137 1156
Fragments arranged by size:
1156 372 137
DrdI GACnn_nn'nnGTC
Cuts at: 0 1635 1665
Size: 1635 30
EaeI y'GGCC_r
Cuts at: 0 5 77 546 1037 1304 1665
Size: 5 72 469 491 267 361
Fragments arranged by size:
491 469 361 267 72 5
EagI C'GGCC_G
Cuts at: 0 5 1665
Size: 5 1660
EarI CTCTTCn'nnn_
Cuts at: 0 67 643 1424 1579 1665
Size: 67 576 781 155 86
Fragments arranged by size:
781 576 155 86 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 1665
Size: 166 1499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 675 855 1016 1645 1662 1665
Size: 154 521 180 161 629 17 3
Fragments arranged by size:
629 521 180 161 154 17 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 324 625 1665
Size: 324 301 1040
Fragments arranged by size:
1040 324 301
EcoO109I rG'GnC_Cy
Cuts at: 0 351 1234 1665
Size: 351 883 431
Fragments arranged by size:
883 431 351
EcoRV GAT'ATC
Cuts at: 0 440 1665
Size: 440 1225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 687 1665
Size: 264 423 978
Fragments arranged by size:
978 423 264
HaeII r_GCGC'y
Cuts at: 0 644 759 1031 1665
Size: 644 115 272 634
Fragments arranged by size:
644 634 272 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 700 732 1369 1401 1423 1455
Size: 340 32 328 32 637 32 22 32
Cuts at: 1455 1665
Size: 210
Fragments arranged by size:
637 340 328 210 32 32 32 32
22
HincII GTy'rAC
Cuts at: 0 1092 1665
Size: 1092 573
MscI TGG'CCA
Cuts at: 0 79 1039 1306 1665
Size: 79 960 267 359
Fragments arranged by size:
960 359 267 79
MslI CAynn'nnrTG
Cuts at: 0 199 256 1665
Size: 199 57 1409
Fragments arranged by size:
1409 199 57
MspA1I CmG'CkG
Cuts at: 0 793 1005 1064 1209 1665
Size: 793 212 59 145 456
Fragments arranged by size:
793 456 212 145 59
NotI GC'GGCC_GC
Cuts at: 0 5 1665
Size: 5 1660
NspI r_CATG'y
Cuts at: 0 891 1665
Size: 891 774
PfoI T'CCnGG_A
Cuts at: 0 1622 1639 1665
Size: 1622 17 26
Fragments arranged by size:
1622 26 17
PspOMI G'GGCC_C
Cuts at: 0 487 1665
Size: 487 1178
PvuII CAG'CTG
Cuts at: 0 1209 1665
Size: 1209 456
SfcI C'TryA_G
Cuts at: 0 1403 1665
Size: 1403 262
SmaI CCC'GGG
Cuts at: 0 43 1665
Size: 43 1622
SmlI C'TyrA_G
Cuts at: 0 661 1665
Size: 661 1004
StuI AGG'CCT
Cuts at: 0 319 532 1665
Size: 319 213 1133
Fragments arranged by size:
1133 319 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1277 1665
Size: 1277 388
TatI w'GTAC_w
Cuts at: 0 1007 1394 1665
Size: 1007 387 271
Fragments arranged by size:
1007 387 271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 632 879 1665
Size: 632 247 786
Fragments arranged by size:
786 632 247
XmaI C'CCGG_G
Cuts at: 0 41 1665
Size: 41 1624
Enzymes that do cut:
AccI AcuI AfeI AhdI AleI AlwNI ApaI AvaI
BamHI BanI BanII BbsI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BsrFI BsrGI BssHII BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII MscI
MslI MspA1I NotI NspI PfoI PspOMI PvuII SfcI
SmaI SmlI StuI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BmtI BsaBI
BsaWI BsgI BsiWI BsmBI BspEI BspHI BspMI BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoICRI EcoRI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI