Restriction map for
pF1KB5507
(Linear) (Six-base) MAPSORT of: KIBB5507.seq Check: 6694 from: 1 to: 1788
KIBB5507.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 876 1788
Size: 271 605 912
Fragments arranged by size:
912 605 271
AflIII A'CryG_T
Cuts at: 0 1454 1788
Size: 1454 334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1174 1788
Size: 1174 614
AlwNI CAG_nnn'CTG
Cuts at: 0 400 1014 1542 1788
Size: 400 614 528 246
Fragments arranged by size:
614 528 400 246
ApaLI G'TGCA_C
Cuts at: 0 458 1788
Size: 458 1330
ApoI r'AATT_y
Cuts at: 0 306 971 1400 1788
Size: 306 665 429 388
Fragments arranged by size:
665 429 388 306
AvaI C'yCGr_G
Cuts at: 0 7 548 880 1788
Size: 7 541 332 908
Fragments arranged by size:
908 541 332 7
BamHI G'GATC_C
Cuts at: 0 846 1788
Size: 846 942
BanII G_rGCy'C
Cuts at: 0 8 68 1788
Size: 8 60 1720
Fragments arranged by size:
1720 60 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1322 1788
Size: 1322 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1137 1171 1788
Size: 1137 34 617
Fragments arranged by size:
1137 617 34
BclI T'GATC_A
Cuts at: 0 831 1788
Size: 831 957
BglII A'GATC_T
Cuts at: 0 906 1788
Size: 906 882
BlpI GC'TnA_GC
Cuts at: 0 67 1788
Size: 67 1721
Bme1580I G_kGCm'C
Cuts at: 0 462 1788
Size: 462 1326
BmgBI CAC'GTC
Cuts at: 0 296 1455 1788
Size: 296 1159 333
Fragments arranged by size:
1159 333 296
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 1288 1422 1788
Size: 189 1099 134 366
Fragments arranged by size:
1099 366 189 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1036 1126 1406 1788
Size: 853 183 90 280 382
Fragments arranged by size:
853 382 280 183 90
Bpu10I CC'TnA_GC
Cuts at: 0 235 1529 1788
Size: 235 1294 259
Fragments arranged by size:
1294 259 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1173 1203 1788
Size: 1173 30 585
Fragments arranged by size:
1173 585 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1373 1620 1788
Size: 1373 247 168
Fragments arranged by size:
1373 247 168
BseYI C'CCAG_C
Cuts at: 0 393 1788
Size: 393 1395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1537 1788
Size: 1537 251
BsiEI CG_ry'CG
Cuts at: 0 37 98 1788
Size: 37 61 1690
Fragments arranged by size:
1690 61 37
BsiHKAI G_wGCw'C
Cuts at: 0 462 998 1788
Size: 462 536 790
Fragments arranged by size:
790 536 462
BsmI GAATG_Cn'
Cuts at: 0 786 1788
Size: 786 1002
Bsp1286I G_dGCh'C
Cuts at: 0 8 68 462 998 1788
Size: 8 60 394 536 790
Fragments arranged by size:
790 536 394 60 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 410 1328 1788
Size: 410 918 460
Fragments arranged by size:
918 460 410
BsrBI CCG'CTC
Cuts at: 0 1492 1788
Size: 1492 296
BsrDI GCAATG_nn'
Cuts at: 0 1496 1788
Size: 1496 292
BsrFI r'CCGG_y
Cuts at: 0 1280 1788
Size: 1280 508
BssSI C'ACGA_G
Cuts at: 0 287 757 1788
Size: 287 470 1031
Fragments arranged by size:
1031 470 287
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1542 1788
Size: 1542 246
BstEII G'GTnAC_C
Cuts at: 0 379 767 1788
Size: 379 388 1021
Fragments arranged by size:
1021 388 379
BstYI r'GATC_y
Cuts at: 0 388 573 846 906 1788
Size: 388 185 273 60 882
Fragments arranged by size:
882 388 273 185 60
BtgI C'CryG_G
Cuts at: 0 37 1167 1788
Size: 37 1130 621
Fragments arranged by size:
1130 621 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 888 1788
Size: 888 900
BtsI GCAGTG_nn'
Cuts at: 0 462 1477 1788
Size: 462 1015 311
Fragments arranged by size:
1015 462 311
DrdI GACnn_nn'nnGTC
Cuts at: 0 347 1788
Size: 347 1441
EaeI y'GGCC_r
Cuts at: 0 95 1493 1788
Size: 95 1398 295
Fragments arranged by size:
1398 295 95
EagI C'GGCC_G
Cuts at: 0 95 1788
Size: 95 1693
EarI CTCTTCn'nnn_
Cuts at: 0 629 830 1553 1731 1788
Size: 629 201 723 178 57
Fragments arranged by size:
723 629 201 178 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 853 876 1036 1126 1406 1788
Size: 271 582 23 160 90 280 382
Fragments arranged by size:
582 382 280 271 160 90 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1584 1788
Size: 1584 204
EcoO109I rG'GnC_Cy
Cuts at: 0 88 1131 1539 1788
Size: 88 1043 408 249
Fragments arranged by size:
1043 408 249 88
EcoRI G'AATT_C
Cuts at: 0 1400 1788
Size: 1400 388
EcoRV GAT'ATC
Cuts at: 0 927 1056 1788
Size: 927 129 732
Fragments arranged by size:
927 732 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 844 991 1788
Size: 844 147 797
Fragments arranged by size:
844 797 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 652 684 1788
Size: 373 32 247 32 1104
Fragments arranged by size:
1104 373 247 32 32
HincII GTy'rAC
Cuts at: 0 425 698 1788
Size: 425 273 1090
Fragments arranged by size:
1090 425 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1788
Size: 92 1696
MslI CAynn'nnrTG
Cuts at: 0 487 757 1001 1681 1788
Size: 487 270 244 680 107
Fragments arranged by size:
680 487 270 244 107
MspA1I CmG'CkG
Cuts at: 0 39 810 1788
Size: 39 771 978
Fragments arranged by size:
978 771 39
NcoI C'CATG_G
Cuts at: 0 1167 1788
Size: 1167 621
NspI r_CATG'y
Cuts at: 0 1680 1788
Size: 1680 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 1788
Size: 1564 32 192
Fragments arranged by size:
1564 192 32
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1788
Size: 1131 657
PstI C_TGCA'G
Cuts at: 0 1325 1538 1788
Size: 1325 213 250
Fragments arranged by size:
1325 250 213
PvuII CAG'CTG
Cuts at: 0 810 1788
Size: 810 978
SacII CC_GC'GG
Cuts at: 0 40 1788
Size: 40 1748
SanDI GG'GwC_CC
Cuts at: 0 1131 1788
Size: 1131 657
SapI GCTCTTCn'nnn_
Cuts at: 0 830 1731 1788
Size: 830 901 57
Fragments arranged by size:
901 830 57
SbfI CC_TGCA'GG
Cuts at: 0 1325 1788
Size: 1325 463
SexAI A'CCwGG_T
Cuts at: 0 1271 1788
Size: 1271 517
SfcI C'TryA_G
Cuts at: 0 786 798 1321 1534 1788
Size: 786 12 523 213 254
Fragments arranged by size:
786 523 254 213 12
SmlI C'TyrA_G
Cuts at: 0 880 1788
Size: 880 908
SphI G_CATG'C
Cuts at: 0 1680 1788
Size: 1680 108
StuI AGG'CCT
Cuts at: 0 595 1788
Size: 595 1193
StyI C'CwwG_G
Cuts at: 0 299 1167 1788
Size: 299 868 621
Fragments arranged by size:
868 621 299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1173 1205 1788
Size: 1173 32 583
Fragments arranged by size:
1173 583 32
XhoI C'TCGA_G
Cuts at: 0 880 1788
Size: 880 908
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI BamHI
BanII BbsI BcgI BclI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstYI BtgI BtgZI BtsI DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I HincII MmeI MslI MspA1I NcoI NspI PpiI
PpuMI PstI PvuII SacII SanDI SapI SbfI SexAI
SfcI SmlI SphI StuI StyI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BfrBI BglI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EciI EcoICRI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XcmI XmaI XmnI ZraI