Restriction map for
pF1KB5511
(Linear) (Six-base) MAPSORT of: KIBB5511.seq Check: 2966 from: 1 to: 2181
KIBB5511.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 205 1553 2181
Size: 205 1348 628
Fragments arranged by size:
1348 628 205
AatII G_ACGT'C
Cuts at: 0 211 2181
Size: 211 1970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 361 1119 2181
Size: 262 99 758 1062
Fragments arranged by size:
1062 758 262 99
AfeI AGC'GCT
Cuts at: 0 136 2181
Size: 136 2045
AleI CACnn'nnGTG
Cuts at: 0 1687 2181
Size: 1687 494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1119 1399 2181
Size: 1119 280 782
Fragments arranged by size:
1119 782 280
AlwNI CAG_nnn'CTG
Cuts at: 0 627 2181
Size: 627 1554
ApaI G_GGCC'C
Cuts at: 0 329 1054 1905 2181
Size: 329 725 851 276
Fragments arranged by size:
851 725 329 276
ApoI r'AATT_y
Cuts at: 0 1840 2181
Size: 1840 341
AvaI C'yCGr_G
Cuts at: 0 329 1579 2181
Size: 329 1250 602
Fragments arranged by size:
1250 602 329
AvrII C'CTAG_G
Cuts at: 0 177 2181
Size: 177 2004
BanI G'GyrC_C
Cuts at: 0 2146 2181
Size: 2146 35
BanII G_rGCy'C
Cuts at: 0 329 338 392 460 1054 1169 1263 1905
Size: 329 9 54 68 594 115 94 642
Cuts at: 1905 2073 2132 2181
Size: 168 59 49
Fragments arranged by size:
642 594 329 168 115 94 68 59
54 49 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 2181
Size: 366 1815
BbvCI CC'TCA_GC
Cuts at: 0 383 490 2181
Size: 383 107 1691
Fragments arranged by size:
1691 383 107
BciVI GTATCCnnnnn_n'
Cuts at: 0 434 2181
Size: 434 1747
BclI T'GATC_A
Cuts at: 0 1367 2181
Size: 1367 814
BglI GCCn_nnn'nGGC
Cuts at: 0 833 2181
Size: 833 1348
BglII A'GATC_T
Cuts at: 0 554 1407 2181
Size: 554 853 774
Fragments arranged by size:
853 774 554
BlpI GC'TnA_GC
Cuts at: 0 138 2181
Size: 138 2043
Bme1580I G_kGCm'C
Cuts at: 0 329 1054 1346 1383 1905 2149 2181
Size: 329 725 292 37 522 244 32
Fragments arranged by size:
725 522 329 292 244 37 32
BmgBI CAC'GTC
Cuts at: 0 309 1152 2181
Size: 309 843 1029
Fragments arranged by size:
1029 843 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 1899 2181
Size: 1899 282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1968 2181
Size: 1968 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 433 482 1870 2181
Size: 274 159 49 1388 311
Fragments arranged by size:
1388 311 274 159 49
Bpu10I CC'TnA_GC
Cuts at: 0 383 490 992 2181
Size: 383 107 502 1189
Fragments arranged by size:
1189 502 383 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 814 2181
Size: 640 174 1367
Fragments arranged by size:
1367 640 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 409 1742 2181
Size: 103 306 1333 439
Fragments arranged by size:
1333 439 306 103
BsaAI yAC'GTr
Cuts at: 0 1302 2181
Size: 1302 879
BsaBI GATnn'nnATC
Cuts at: 0 589 2181
Size: 589 1592
BsaHI Gr'CG_yC
Cuts at: 0 62 208 1648 2181
Size: 62 146 1440 533
Fragments arranged by size:
1440 533 146 62
BsaWI w'CCGG_w
Cuts at: 0 426 2181
Size: 426 1755
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 2181
Size: 406 30 1745
Fragments arranged by size:
1745 406 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 1726 2181
Size: 400 1326 455
Fragments arranged by size:
1326 455 400
BseYI C'CCAG_C
Cuts at: 0 27 2181
Size: 27 2154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1107 1289 1333 1531 2181
Size: 783 324 182 44 198 650
Fragments arranged by size:
783 650 324 198 182 44
BsiHKAI G_wGCw'C
Cuts at: 0 338 392 656 1724 2181
Size: 338 54 264 1068 457
Fragments arranged by size:
1068 457 338 264 54
BsmI GAATG_Cn'
Cuts at: 0 1077 2181
Size: 1077 1104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1639 2181
Size: 1639 542
Bsp1286I G_dGCh'C
Cuts at: 0 329 338 392 460 656 1054 1169 1263
Size: 329 9 54 68 196 398 115 94
Cuts at: 1263 1346 1383 1724 1905 2073 2132 2149 2181
Size: 83 37 341 181 168 59 17 32
Fragments arranged by size:
398 341 329 196 181 168 115 94
83 68 59 54 37 32 17 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 205 1553 2181
Size: 205 1348 628
Fragments arranged by size:
1348 628 205
BsrBI CCG'CTC
Cuts at: 0 1827 2181
Size: 1827 354
BsrDI GCAATG_nn'
Cuts at: 0 1208 1856 2181
Size: 1208 648 325
Fragments arranged by size:
1208 648 325
BsrFI r'CCGG_y
Cuts at: 0 1106 1493 2181
Size: 1106 387 688
Fragments arranged by size:
1106 688 387
BssSI C'ACGA_G
Cuts at: 0 973 2181
Size: 973 1208
BstBI TT'CG_AA
Cuts at: 0 788 2181
Size: 788 1393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 172 864 1089 1626 2181
Size: 172 692 225 537 555
Fragments arranged by size:
692 555 537 225 172
BstYI r'GATC_y
Cuts at: 0 161 276 554 1407 2181
Size: 161 115 278 853 774
Fragments arranged by size:
853 774 278 161 115
Bsu36I CC'TnA_GG
Cuts at: 0 1055 2181
Size: 1055 1126
BtgI C'CryG_G
Cuts at: 0 720 857 2181
Size: 720 137 1324
Fragments arranged by size:
1324 720 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1980 2142 2181
Size: 1980 162 39
Fragments arranged by size:
1980 162 39
BtsI GCAGTG_nn'
Cuts at: 0 1383 2181
Size: 1383 798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 879 914 2181
Size: 879 35 1267
Fragments arranged by size:
1267 879 35
DraIII CAC_nnn'GTG
Cuts at: 0 806 2181
Size: 806 1375
EaeI y'GGCC_r
Cuts at: 0 44 98 503 1506 1728 2181
Size: 44 54 405 1003 222 453
Fragments arranged by size:
1003 453 405 222 54 44
EarI CTCTTCn'nnn_
Cuts at: 0 295 568 995 1417 2181
Size: 295 273 427 422 764
Fragments arranged by size:
764 427 422 295 273
EcoICRI GAG'CTC
Cuts at: 0 336 390 2181
Size: 336 54 1791
Fragments arranged by size:
1791 336 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 274 361 433 482 1119 1870 2181
Size: 262 12 87 72 49 637 751 311
Fragments arranged by size:
751 637 311 262 87 72 49 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 1561 2181
Size: 181 1380 620
Fragments arranged by size:
1380 620 181
EcoO109I rG'GnC_Cy
Cuts at: 0 325 326 1051 1901 2181
Size: 325 1 725 850 280
Fragments arranged by size:
850 725 325 280 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 576 2181
Size: 576 1605
FspI TGC'GCA
Cuts at: 0 823 2181
Size: 823 1358
HaeII r_GCGC'y
Cuts at: 0 138 172 2181
Size: 138 34 2009
Fragments arranged by size:
2009 138 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 408 440 455 487 947 979 1018 1050
Size: 408 32 15 32 460 32 39 32
Cuts at: 1050 1384 1416 2181
Size: 334 32 765
Fragments arranged by size:
765 460 408 334 39 32 32 32
32 32 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1215 2181
Size: 1215 966
MscI TGG'CCA
Cuts at: 0 100 1508 1730 2181
Size: 100 1408 222 451
Fragments arranged by size:
1408 451 222 100
MslI CAynn'nnrTG
Cuts at: 0 1687 1804 1970 2120 2181
Size: 1687 117 166 150 61
Fragments arranged by size:
1687 166 150 117 61
MspA1I CmG'CkG
Cuts at: 0 540 608 918 1121 1218 2181
Size: 540 68 310 203 97 963
Fragments arranged by size:
963 540 310 203 97 68
NaeI GCC'GGC
Cuts at: 0 1108 2181
Size: 1108 1073
NcoI C'CATG_G
Cuts at: 0 720 2181
Size: 720 1461
NgoMIV G'CCGG_C
Cuts at: 0 1106 2181
Size: 1106 1075
NspI r_CATG'y
Cuts at: 0 954 2181
Size: 954 1227
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 2181
Size: 627 1554
PspOMI G'GGCC_C
Cuts at: 0 325 1050 1901 2181
Size: 325 725 851 280
Fragments arranged by size:
851 725 325 280
PstI C_TGCA'G
Cuts at: 0 746 1400 2181
Size: 746 654 781
Fragments arranged by size:
781 746 654
PvuII CAG'CTG
Cuts at: 0 540 1121 2181
Size: 540 581 1060
Fragments arranged by size:
1060 581 540
SacI G_AGCT'C
Cuts at: 0 338 392 2181
Size: 338 54 1789
Fragments arranged by size:
1789 338 54
SfcI C'TryA_G
Cuts at: 0 742 1396 2181
Size: 742 654 785
Fragments arranged by size:
785 742 654
SmaI CCC'GGG
Cuts at: 0 331 2181
Size: 331 1850
SmlI C'TyrA_G
Cuts at: 0 655 829 2181
Size: 655 174 1352
Fragments arranged by size:
1352 655 174
StyI C'CwwG_G
Cuts at: 0 177 638 720 1503 2074 2181
Size: 177 461 82 783 571 107
Fragments arranged by size:
783 571 461 177 107 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 1177 1555 2181
Size: 127 1050 378 626
Fragments arranged by size:
1050 626 378 127
TatI w'GTAC_w
Cuts at: 0 1598 1853 2181
Size: 1598 255 328
Fragments arranged by size:
1598 328 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 426 558 628 2081 2181
Size: 366 60 132 70 1453 100
Fragments arranged by size:
1453 366 132 100 70 60
XmaI C'CCGG_G
Cuts at: 0 329 2181
Size: 329 1852
XmnI GAAnn'nnTTC
Cuts at: 0 346 2181
Size: 346 1835
ZraI GAC'GTC
Cuts at: 0 209 2181
Size: 209 1972
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PspOMI PstI PvuII SacI SfcI SmaI SmlI
StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI
BfrBI BmtI BsiEI BsiWI BspEI BspHI BsrGI BssHII
BstAPI BstEII BstZ17I ClaI DraI DrdI EagI EciI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI