Restriction map for
pF1KB5513
(Linear) (Six-base) MAPSORT of: KIBB5513.seq Check: 8557 from: 1 to: 2100
KIBB5513.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
Acc65I G'GTAC_C
Cuts at: 0 1858 2100
Size: 1858 242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1698 1801 2100
Size: 1698 103 299
Fragments arranged by size:
1698 299 103
AfeI AGC'GCT
Cuts at: 0 1381 2100
Size: 1381 719
AflIII A'CryG_T
Cuts at: 0 785 2100
Size: 785 1315
AlwNI CAG_nnn'CTG
Cuts at: 0 771 840 1161 2100
Size: 771 69 321 939
Fragments arranged by size:
939 771 321 69
ApaI G_GGCC'C
Cuts at: 0 194 2100
Size: 194 1906
ApoI r'AATT_y
Cuts at: 0 967 1826 1866 2100
Size: 967 859 40 234
Fragments arranged by size:
967 859 234 40
AvaI C'yCGr_G
Cuts at: 0 209 365 2041 2100
Size: 209 156 1676 59
Fragments arranged by size:
1676 209 156 59
AvrII C'CTAG_G
Cuts at: 0 303 2100
Size: 303 1797
BamHI G'GATC_C
Cuts at: 0 2037 2100
Size: 2037 63
BanI G'GyrC_C
Cuts at: 0 592 1858 2100
Size: 592 1266 242
Fragments arranged by size:
1266 592 242
BanII G_rGCy'C
Cuts at: 0 58 194 303 1105 1232 2066 2100
Size: 58 136 109 802 127 834 34
Fragments arranged by size:
834 802 136 127 109 58 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 2100
Size: 620 1480
BbvCI CC'TCA_GC
Cuts at: 0 1342 2100
Size: 1342 758
BclI T'GATC_A
Cuts at: 0 1163 2100
Size: 1163 937
BglI GCCn_nnn'nGGC
Cuts at: 0 22 1621 2100
Size: 22 1599 479
Fragments arranged by size:
1599 479 22
BglII A'GATC_T
Cuts at: 0 239 1016 1640 2100
Size: 239 777 624 460
Fragments arranged by size:
777 624 460 239
Bme1580I G_kGCm'C
Cuts at: 0 194 785 2100
Size: 194 591 1315
Fragments arranged by size:
1315 591 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 1611 2100
Size: 249 1362 489
Fragments arranged by size:
1362 489 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 183 549 562 1033 1113 1192 2100
Size: 139 44 366 13 471 80 79 908
Fragments arranged by size:
908 471 366 139 80 79 44 13
Bpu10I CC'TnA_GC
Cuts at: 0 1342 2100
Size: 1342 758
BsaBI GATnn'nnATC
Cuts at: 0 847 2100
Size: 847 1253
BsaWI w'CCGG_w
Cuts at: 0 622 799 2100
Size: 622 177 1301
Fragments arranged by size:
1301 622 177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 284 823 943 1189 1192 1207 1327 2100
Size: 284 539 120 246 3 15 120 773
Fragments arranged by size:
773 539 284 246 120 120 15 3
BseYI C'CCAG_C
Cuts at: 0 51 1773 2100
Size: 51 1722 327
Fragments arranged by size:
1722 327 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 2100
Size: 1197 903
BsiEI CG_ry'CG
Cuts at: 0 44 2100
Size: 44 2056
BsiHKAI G_wGCw'C
Cuts at: 0 1415 2066 2100
Size: 1415 651 34
Fragments arranged by size:
1415 651 34
BsmI GAATG_Cn'
Cuts at: 0 504 2100
Size: 504 1596
Bsp1286I G_dGCh'C
Cuts at: 0 58 194 303 785 1105 1232 1415 2066
Size: 58 136 109 482 320 127 183 651
Cuts at: 2066 2100
Size: 34
Fragments arranged by size:
651 482 320 183 136 127 109 58
34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1891 2100
Size: 1891 209
BsrBI CCG'CTC
Cuts at: 0 1208 2100
Size: 1208 892
BsrDI GCAATG_nn'
Cuts at: 0 329 629 2100
Size: 329 300 1471
Fragments arranged by size:
1471 329 300
BssSI C'ACGA_G
Cuts at: 0 61 1450 2100
Size: 61 1389 650
Fragments arranged by size:
1389 650 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 331 2100
Size: 331 1769
BstBI TT'CG_AA
Cuts at: 0 611 1466 2100
Size: 611 855 634
Fragments arranged by size:
855 634 611
BstEII G'GTnAC_C
Cuts at: 0 795 2100
Size: 795 1305
BstYI r'GATC_y
Cuts at: 0 239 410 1016 1640 2037 2100
Size: 239 171 606 624 397 63
Fragments arranged by size:
624 606 397 239 171 63
Bsu36I CC'TnA_GG
Cuts at: 0 994 2100
Size: 994 1106
BtgI C'CryG_G
Cuts at: 0 233 740 1109 2100
Size: 233 507 369 991
Fragments arranged by size:
991 507 369 233
ClaI AT'CG_AT
Cuts at: 0 1970 2100
Size: 1970 130
EaeI y'GGCC_r
Cuts at: 0 14 41 155 2100
Size: 14 27 114 1945
Fragments arranged by size:
1945 114 27 14
EagI C'GGCC_G
Cuts at: 0 41 2100
Size: 41 2059
EarI CTCTTCn'nnn_
Cuts at: 0 139 1556 2100
Size: 139 1417 544
Fragments arranged by size:
1417 544 139
EcoICRI GAG'CTC
Cuts at: 0 2064 2100
Size: 2064 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 183 549 562 1033 1113 1192 1698
Size: 139 44 366 13 471 80 79 506
Cuts at: 1698 1801 2100
Size: 103 299
Fragments arranged by size:
506 471 366 299 139 103 80 79
44 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 1405 2100
Size: 85 1320 695
Fragments arranged by size:
1320 695 85
EcoO109I rG'GnC_Cy
Cuts at: 0 141 190 191 2100
Size: 141 49 1 1909
Fragments arranged by size:
1909 141 49 1
EcoRI G'AATT_C
Cuts at: 0 967 1866 2100
Size: 967 899 234
Fragments arranged by size:
967 899 234
EcoRV GAT'ATC
Cuts at: 0 1698 2100
Size: 1698 402
HaeII r_GCGC'y
Cuts at: 0 103 231 536 740 1383 1692 2100
Size: 103 128 305 204 643 309 408
Fragments arranged by size:
643 408 309 305 204 128 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 1687 1719 1902 1934 2059 2091
Size: 1137 32 518 32 183 32 125 32
Cuts at: 2091 2100
Size: 9
Fragments arranged by size:
1137 518 183 125 32 32 32 32
9
HincII GTy'rAC
Cuts at: 0 1743 1848 1921 2100
Size: 1743 105 73 179
Fragments arranged by size:
1743 179 105 73
KpnI G_GTAC'C
Cuts at: 0 1862 2100
Size: 1862 238
MluI A'CGCG_T
Cuts at: 0 785 2100
Size: 785 1315
MslI CAynn'nnrTG
Cuts at: 0 959 1078 1450 2100
Size: 959 119 372 650
Fragments arranged by size:
959 650 372 119
MspA1I CmG'CkG
Cuts at: 0 742 1111 2034 2100
Size: 742 369 923 66
Fragments arranged by size:
923 742 369 66
NspI r_CATG'y
Cuts at: 0 1278 1730 1749 2100
Size: 1278 452 19 351
Fragments arranged by size:
1278 452 351 19
PasI CC'CwG_GG
Cuts at: 0 171 860 2100
Size: 171 689 1240
Fragments arranged by size:
1240 689 171
PfoI T'CCnGG_A
Cuts at: 0 137 392 1403 2100
Size: 137 255 1011 697
Fragments arranged by size:
1011 697 255 137
PpuMI rG'GwC_Cy
Cuts at: 0 141 2100
Size: 141 1959
PspOMI G'GGCC_C
Cuts at: 0 190 2100
Size: 190 1910
PspXI vC'TCGA_Gb
Cuts at: 0 365 2100
Size: 365 1735
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 2100
Size: 1141 32 927
Fragments arranged by size:
1141 927 32
PstI C_TGCA'G
Cuts at: 0 1661 2100
Size: 1661 439
PvuII CAG'CTG
Cuts at: 0 2034 2100
Size: 2034 66
SacI G_AGCT'C
Cuts at: 0 2066 2100
Size: 2066 34
SacII CC_GC'GG
Cuts at: 0 743 1112 2100
Size: 743 369 988
Fragments arranged by size:
988 743 369
SapI GCTCTTCn'nnn_
Cuts at: 0 139 2100
Size: 139 1961
ScaI AGT'ACT
Cuts at: 0 2096 2100
Size: 2096 4
SfcI C'TryA_G
Cuts at: 0 832 917 1657 2100
Size: 832 85 740 443
Fragments arranged by size:
832 740 443 85
SmaI CCC'GGG
Cuts at: 0 211 2100
Size: 211 1889
SmlI C'TyrA_G
Cuts at: 0 365 2100
Size: 365 1735
StyI C'CwwG_G
Cuts at: 0 294 303 479 711 2100
Size: 294 9 176 232 1389
Fragments arranged by size:
1389 294 232 176 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 783 2100
Size: 435 348 1317
Fragments arranged by size:
1317 435 348
TatI w'GTAC_w
Cuts at: 0 1442 2094 2100
Size: 1442 652 6
Fragments arranged by size:
1442 652 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 262 2100
Size: 262 1838
XhoI C'TCGA_G
Cuts at: 0 365 2100
Size: 365 1735
XmaI C'CCGG_G
Cuts at: 0 209 2100
Size: 209 1891
XmnI GAAnn'nnTTC
Cuts at: 0 1138 1786 2100
Size: 1138 648 314
Fragments arranged by size:
1138 648 314
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BclI BglI
BglII Bme1580I BmrI BpmI Bpu10I BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
ClaI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII KpnI MluI MslI
MspA1I NspI PasI PfoI PpuMI PspOMI PspXI PsrI
PstI PvuII SacI SacII SapI ScaI SfcI SmaI
SmlI StyI TaqII TatI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BbeI
BcgI BciVI BfrBI BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaHI BsaXI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BstXI BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EciI FalI FseI FspI FspAI
HindIII HpaI KasI MfeI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PvuI
RsrII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI ZraI