(Linear) (Six-base) MAPSORT of: KIBB5514.seq  Check: 2926  from: 1  to: 1341



KIBB5514.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AccI GT'mk_AC

Cuts at:      0     337     728    1341 
   Size:        337     391     613

  Fragments arranged by size: 

                 613     391     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     836    1080    1195    1341 
   Size:        836     244     115     146

  Fragments arranged by size: 

                 836     244     146     115
 
AhdI GACnn_n'nnGTC

Cuts at:      0     915    1341 
   Size:        915     426
 
AlwNI CAG_nnn'CTG

Cuts at:      0      10    1341 
   Size:         10    1331
 
ApoI r'AATT_y

Cuts at:      0     103     697     874     926    1033    1341 
   Size:        103     594     177      52     107     308

  Fragments arranged by size: 

                 594     308     177     107     103      52
 
AseI AT'TA_AT

Cuts at:      0     554     599    1341 
   Size:        554      45     742

  Fragments arranged by size: 

                 742     554      45
 
BanI G'GyrC_C

Cuts at:      0    1271    1341 
   Size:       1271      70
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     818    1341 
   Size:        818     523
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     137    1341 
   Size:         32     105    1204

  Fragments arranged by size: 

                1204     105      32
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      29     218     427     846    1341 
   Size:         29     189     209     419     495

  Fragments arranged by size: 

                 495     419     209     189      29
 
BspHI T'CATG_A

Cuts at:      0    1070    1341 
   Size:       1070     271
 
BsrDI GCAATG_nn'

Cuts at:      0    1289    1341 
   Size:       1289      52
 
BstEII G'GTnAC_C

Cuts at:      0     302    1341 
   Size:        302    1039
 
BstYI r'GATC_y

Cuts at:      0     432     480     819    1054    1078    1341 
   Size:        432      48     339     235      24     263

  Fragments arranged by size: 

                 432     339     263     235      48      24
 
BstZ17I GTA'TAC

Cuts at:      0     729    1341 
   Size:        729     612
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1127    1341 
   Size:       1127     214
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1066    1341 
   Size:       1066     275
 
EarI CTCTTCn'nnn_

Cuts at:      0     662    1341 
   Size:        662     679
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     137     836    1080    1195    1341 
   Size:         32     105     699     244     115     146

  Fragments arranged by size: 

                 699     244     146     115     105      32
 
EcoRV GAT'ATC

Cuts at:      0     648    1341 
   Size:        648     693
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     449     481     901     933    1341 
   Size:        143      32     274      32     420      32     408

  Fragments arranged by size: 

                 420     408     274     143      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1230    1341 
   Size:       1230     111
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1341 
   Size:       1027     314
 
MspA1I CmG'CkG

Cuts at:      0     713    1341 
   Size:        713     628
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     984    1016    1341 
   Size:        984      32     325

  Fragments arranged by size: 

                 984     325      32
 
PstI C_TGCA'G

Cuts at:      0     585    1341 
   Size:        585     756
 
PvuII CAG'CTG

Cuts at:      0     713    1341 
   Size:        713     628
 
SapI GCTCTTCn'nnn_

Cuts at:      0     662    1341 
   Size:        662     679
 
SexAI A'CCwGG_T

Cuts at:      0     968    1341 
   Size:        968     373
 
SfcI C'TryA_G

Cuts at:      0      95     440     581    1341 
   Size:         95     345     141     760

  Fragments arranged by size: 

                 760     345     141      95
 
SspI AAT'ATT

Cuts at:      0     512    1341 
   Size:        512     829
 
StuI AGG'CCT

Cuts at:      0     474     954    1341 
   Size:        474     480     387

  Fragments arranged by size: 

                 480     474     387
 
StyI C'CwwG_G

Cuts at:      0    1277    1341 
   Size:       1277      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     357     389    1341 
   Size:        357      32     952

  Fragments arranged by size: 

                 952     357      32
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI     ApoI     AseI     BanI     BmrI
     BpmI    BseRI    BspHI    BsrDI   BstEII    BstYI  BstZ17I    BtgZI
     DrdI     EarI  Eco57MI    EcoRV    Hin4I   HincII     MmeI   MspA1I
     PsrI     PstI    PvuII     SapI    SexAI     SfcI     SspI     StuI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI