Restriction map for
pF1KB5522
(Linear) (Six-base) MAPSORT of: KIBB5522.seq Check: 8666 from: 1 to: 1146
KIBB5522.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 1146
Size: 855 291
AfeI AGC'GCT
Cuts at: 0 424 1146
Size: 424 722
AlwNI CAG_nnn'CTG
Cuts at: 0 497 1146
Size: 497 649
ApaI G_GGCC'C
Cuts at: 0 8 1146
Size: 8 1138
ApoI r'AATT_y
Cuts at: 0 223 1015 1045 1146
Size: 223 792 30 101
Fragments arranged by size:
792 223 101 30
AvaI C'yCGr_G
Cuts at: 0 993 1146
Size: 993 153
BanI G'GyrC_C
Cuts at: 0 807 913 1146
Size: 807 106 233
Fragments arranged by size:
807 233 106
BanII G_rGCy'C
Cuts at: 0 8 1146
Size: 8 1138
BbsI GAAGACnn'nnnn_
Cuts at: 0 618 783 859 1112 1146
Size: 618 165 76 253 34
Fragments arranged by size:
618 253 165 76 34
BglI GCCn_nnn'nGGC
Cuts at: 0 805 1146
Size: 805 341
Bme1580I G_kGCm'C
Cuts at: 0 8 810 994 1146
Size: 8 802 184 152
Fragments arranged by size:
802 184 152 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 1146
Size: 339 807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1146
Size: 202 944
Bpu10I CC'TnA_GC
Cuts at: 0 784 1146
Size: 784 362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1146
Size: 748 398
BsaI GGTCTCn'nnnn_
Cuts at: 0 51 1102 1146
Size: 51 1051 44
Fragments arranged by size:
1051 51 44
BsaWI w'CCGG_w
Cuts at: 0 968 1146
Size: 968 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1146
Size: 1057 30 59
Fragments arranged by size:
1057 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 1146
Size: 394 752
BseYI C'CCAG_C
Cuts at: 0 500 803 1146
Size: 500 303 343
Fragments arranged by size:
500 343 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 1146
Size: 600 546
BsiHKAI G_wGCw'C
Cuts at: 0 905 1146
Size: 905 241
Bsp1286I G_dGCh'C
Cuts at: 0 8 810 905 994 1146
Size: 8 802 95 89 152
Fragments arranged by size:
802 152 95 89 8
BspEI T'CCGG_A
Cuts at: 0 968 1146
Size: 968 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 1146
Size: 311 835
BsrBI CCG'CTC
Cuts at: 0 588 813 877 998 1146
Size: 588 225 64 121 148
Fragments arranged by size:
588 225 148 121 64
BsrFI r'CCGG_y
Cuts at: 0 1036 1146
Size: 1036 110
BtgI C'CryG_G
Cuts at: 0 620 1146
Size: 620 526
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 396 780 1017 1146
Size: 396 384 237 129
Fragments arranged by size:
396 384 237 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 1116 1146
Size: 1116 30
EarI CTCTTCn'nnn_
Cuts at: 0 484 616 820 874 1143 1146
Size: 484 132 204 54 269 3
Fragments arranged by size:
484 269 204 132 54 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 1146
Size: 463 683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 855 1146
Size: 202 653 291
Fragments arranged by size:
653 291 202
EcoNI CCTnn'n_nnAGG
Cuts at: 0 681 1146
Size: 681 465
EcoO109I rG'GnC_Cy
Cuts at: 0 4 1146
Size: 4 1142
EcoRI G'AATT_C
Cuts at: 0 1045 1146
Size: 1045 101
EcoRV GAT'ATC
Cuts at: 0 69 1146
Size: 69 1077
HaeII r_GCGC'y
Cuts at: 0 426 497 760 1146
Size: 426 71 263 386
Fragments arranged by size:
426 386 263 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1016 1048 1057 1089 1146
Size: 1016 32 9 32 57
Fragments arranged by size:
1016 57 32 32 9
HincII GTy'rAC
Cuts at: 0 60 1134 1146
Size: 60 1074 12
Fragments arranged by size:
1074 60 12
MspA1I CmG'CkG
Cuts at: 0 21 569 1146
Size: 21 548 577
Fragments arranged by size:
577 548 21
NaeI GCC'GGC
Cuts at: 0 1038 1146
Size: 1038 108
NgoMIV G'CCGG_C
Cuts at: 0 1036 1146
Size: 1036 110
PfoI T'CCnGG_A
Cuts at: 0 179 1146
Size: 179 967
PspOMI G'GGCC_C
Cuts at: 0 4 1146
Size: 4 1142
PstI C_TGCA'G
Cuts at: 0 667 1146
Size: 667 479
PvuII CAG'CTG
Cuts at: 0 569 1146
Size: 569 577
SapI GCTCTTCn'nnn_
Cuts at: 0 820 1146
Size: 820 326
ScaI AGT'ACT
Cuts at: 0 883 1146
Size: 883 263
SfcI C'TryA_G
Cuts at: 0 663 1146
Size: 663 483
SmlI C'TyrA_G
Cuts at: 0 763 1146
Size: 763 383
StyI C'CwwG_G
Cuts at: 0 677 725 1146
Size: 677 48 421
Fragments arranged by size:
677 421 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 511 1146
Size: 33 478 635
Fragments arranged by size:
635 478 33
TatI w'GTAC_w
Cuts at: 0 239 659 881 1146
Size: 239 420 222 265
Fragments arranged by size:
420 265 239 222
Tth111I GACn'n_nGTC
Cuts at: 0 55 1146
Size: 55 1091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 1146
Size: 18 1128
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BtgI BtgZI DrdI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
MspA1I NaeI NgoMIV PfoI PspOMI PstI PvuII SapI
ScaI SfcI SmlI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII EaeI EagI
EcoICRI FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI XmnI ZraI