Restriction map for
pF1KB5523
(Linear) (Six-base) MAPSORT of: KIBB5523.seq Check: 9923 from: 1 to: 7416
KIBB5523.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1422 3192 7416
Size: 1422 1770 4224
Fragments arranged by size:
4224 1770 1422
AatII G_ACGT'C
Cuts at: 0 4605 7416
Size: 4605 2811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 179 229 290 542 739 1208 2806
Size: 175 4 50 61 252 197 469 1598
Cuts at: 2806 3439 3482 3497 5161 5584 5746 5920 7416
Size: 633 43 15 1664 423 162 174 1496
Fragments arranged by size:
1664 1598 1496 633 469 423 252 197
175 174 162 61 50 43 15 4
AfeI AGC'GCT
Cuts at: 0 775 1511 3640 4027 7416
Size: 775 736 2129 387 3389
Fragments arranged by size:
3389 2129 775 736 387
AflII C'TTAA_G
Cuts at: 0 1516 7416
Size: 1516 5900
AflIII A'CryG_T
Cuts at: 0 6141 7416
Size: 6141 1275
AhdI GACnn_n'nnGTC
Cuts at: 0 3471 4053 5328 7416
Size: 3471 582 1275 2088
Fragments arranged by size:
3471 2088 1275 582
AleI CACnn'nnGTG
Cuts at: 0 1220 7416
Size: 1220 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1420 2702 2880 3145 4333 4535 6925 7416
Size: 1420 1282 178 265 1188 202 2390 491
Fragments arranged by size:
2390 1420 1282 1188 491 265 202 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7195 7227 7416
Size: 7195 32 189
Fragments arranged by size:
7195 189 32
AlwNI CAG_nnn'CTG
Cuts at: 0 117 540 717 876 1278 2337 2463 2655
Size: 117 423 177 159 402 1059 126 192
Cuts at: 2655 3660 3810 4212 4959 5403 5532 5595 6204
Size: 1005 150 402 747 444 129 63 609
Cuts at: 6204 7296 7416
Size: 1092 120
Fragments arranged by size:
1092 1059 1005 747 609 444 423 402
402 192 177 159 150 129 126 120
117 63
ApaI G_GGCC'C
Cuts at: 0 1701 7245 7416
Size: 1701 5544 171
Fragments arranged by size:
5544 1701 171
ApaLI G'TGCA_C
Cuts at: 0 3939 7416
Size: 3939 3477
ApoI r'AATT_y
Cuts at: 0 568 1582 6979 7416
Size: 568 1014 5397 437
Fragments arranged by size:
5397 1014 568 437
AvaI C'yCGr_G
Cuts at: 0 2948 3092 3910 4388 5582 7301 7416
Size: 2948 144 818 478 1194 1719 115
Fragments arranged by size:
2948 1719 1194 818 478 144 115
BamHI G'GATC_C
Cuts at: 0 4740 4814 6659 7134 7416
Size: 4740 74 1845 475 282
Fragments arranged by size:
4740 1845 475 282 74
BanII G_rGCy'C
Cuts at: 0 1701 1754 1862 2315 2414 5365 7245 7286
Size: 1701 53 108 453 99 2951 1880 41
Cuts at: 7286 7367 7416
Size: 81 49
Fragments arranged by size:
2951 1880 1701 453 108 99 81 53
49 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 929 1247 1484 1535 2771 3470 3929 4418
Size: 929 318 237 51 1236 699 459 489
Cuts at: 4418 4469 6004 6017 7416
Size: 51 1535 13 1399
Fragments arranged by size:
1535 1399 1236 929 699 489 459 318
237 51 51 13
BbvCI CC'TCA_GC
Cuts at: 0 947 2774 4121 6418 7246 7416
Size: 947 1827 1347 2297 828 170
Fragments arranged by size:
2297 1827 1347 947 828 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2032 2066 2752 2786 5169 5203 5686 5720
Size: 2032 34 686 34 2383 34 483 34
Cuts at: 5720 7416
Size: 1696
Fragments arranged by size:
2383 2032 1696 686 483 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 170 7416
Size: 170 7246
BclI T'GATC_A
Cuts at: 0 674 1967 4448 7184 7416
Size: 674 1293 2481 2736 232
Fragments arranged by size:
2736 2481 1293 674 232
BglI GCCn_nnn'nGGC
Cuts at: 0 2287 4393 4897 5041 6163 6295 7240 7416
Size: 2287 2106 504 144 1122 132 945 176
Fragments arranged by size:
2287 2106 1122 945 504 176 144 132
BglII A'GATC_T
Cuts at: 0 906 2124 2811 7416
Size: 906 1218 687 4605
Fragments arranged by size:
4605 1218 906 687
BlpI GC'TnA_GC
Cuts at: 0 1856 7416
Size: 1856 5560
Bme1580I G_kGCm'C
Cuts at: 0 1554 1701 3943 7245 7416
Size: 1554 147 2242 3302 171
Fragments arranged by size:
3302 2242 1554 171 147
BmgBI CAC'GTC
Cuts at: 0 480 2393 5958 7416
Size: 480 1913 3565 1458
Fragments arranged by size:
3565 1913 1458 480
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 2909 3075 3244 7416
Size: 1035 1874 166 169 4172
Fragments arranged by size:
4172 1874 1035 169 166
BmtI G_CTAG'C
Cuts at: 0 1708 4102 7416
Size: 1708 2394 3314
Fragments arranged by size:
3314 2394 1708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5856 7416
Size: 5856 1560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 562 919 1378 2131 2677 3127 3370
Size: 187 375 357 459 753 546 450 243
Cuts at: 3370 3490 3981 4153 4309 5902 6259 6544 6555
Size: 120 491 172 156 1593 357 285 11
Cuts at: 6555 6592 6943 7416
Size: 37 351 473
Fragments arranged by size:
1593 753 546 491 473 459 450 375
357 357 351 285 243 187 172 156
120 37 11
Bpu10I CC'TnA_GC
Cuts at: 0 947 2774 4033 4121 4905 6418 7246 7416
Size: 947 1827 1259 88 784 1513 828 170
Fragments arranged by size:
1827 1513 1259 947 828 784 170 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1738 2290 7416
Size: 1738 552 5126
Fragments arranged by size:
5126 1738 552
BsaI GGTCTCn'nnnn_
Cuts at: 0 848 6011 6523 6661 7416
Size: 848 5163 512 138 755
Fragments arranged by size:
5163 848 755 512 138
BsaAI yAC'GTr
Cuts at: 0 3060 7269 7344 7386 7416
Size: 3060 4209 75 42 30
Fragments arranged by size:
4209 3060 75 42 30
BsaBI GATnn'nnATC
Cuts at: 0 128 7416
Size: 128 7288
BsaHI Gr'CG_yC
Cuts at: 0 108 4602 6401 7416
Size: 108 4494 1799 1015
Fragments arranged by size:
4494 1799 1015 108
BsaWI w'CCGG_w
Cuts at: 0 4114 4151 4811 7416
Size: 4114 37 660 2605
Fragments arranged by size:
4114 2605 660 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 281 311 7309 7339 7416
Size: 281 30 6998 30 77
Fragments arranged by size:
6998 281 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 1354 2401 3124 3364 3508 5341 5530
Size: 679 675 1047 723 240 144 1833 189
Cuts at: 5530 5845 6031 6346 6402 6541 6616 6673 6832
Size: 315 186 315 56 139 75 57 159
Cuts at: 6832 6979 7234 7294 7416
Size: 147 255 60 122
Fragments arranged by size:
1833 1047 723 679 675 315 315 255
240 189 186 159 147 144 139 122
75 60 57 56
BseYI C'CCAG_C
Cuts at: 0 528 1124 1180 1700 2639 2675 6067 6632
Size: 528 596 56 520 939 36 3392 565
Cuts at: 6632 6728 6872 6897 7416
Size: 96 144 25 519
Fragments arranged by size:
3392 939 596 565 528 520 519 144
96 56 36 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 364 475 2158 3520 4001 4301 5281
Size: 295 69 111 1683 1362 481 300 980
Cuts at: 5281 5416 5772 6052 7416
Size: 135 356 280 1364
Fragments arranged by size:
1683 1364 1362 980 481 356 300 295
280 135 111 69
BsiEI CG_ry'CG
Cuts at: 0 3033 5379 7416
Size: 3033 2346 2037
Fragments arranged by size:
3033 2346 2037
BsiHKAI G_wGCw'C
Cuts at: 0 1217 1754 1862 3386 3943 5552 6854 7286
Size: 1217 537 108 1524 557 1609 1302 432
Cuts at: 7286 7367 7416
Size: 81 49
Fragments arranged by size:
1609 1524 1302 1217 557 537 432 108
81 49
BsiWI C'GTAC_G
Cuts at: 0 3056 7416
Size: 3056 4360
BsmI GAATG_Cn'
Cuts at: 0 2547 7416
Size: 2547 4869
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2383 7329 7416
Size: 2383 4946 87
Fragments arranged by size:
4946 2383 87
Bsp1286I G_dGCh'C
Cuts at: 0 1217 1554 1701 1754 1862 2315 2414 3386
Size: 1217 337 147 53 108 453 99 972
Cuts at: 3386 3943 5365 5552 6854 7245 7286 7367 7416
Size: 557 1422 187 1302 391 41 81 49
Fragments arranged by size:
1422 1302 1217 972 557 453 391 337
187 147 108 99 81 53 49 41
BspHI T'CATG_A
Cuts at: 0 600 6182 7181 7416
Size: 600 5582 999 235
Fragments arranged by size:
5582 999 600 235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 1412 1422 3192 3363 3935 4028 5093
Size: 51 1361 10 1770 171 572 93 1065
Cuts at: 5093 7082 7416
Size: 1989 334
Fragments arranged by size:
1989 1770 1361 1065 572 334 171 93
51 10
BsrBI CCG'CTC
Cuts at: 0 1348 3337 6410 7400 7416
Size: 1348 1989 3073 990 16
Fragments arranged by size:
3073 1989 1348 990 16
BsrDI GCAATG_nn'
Cuts at: 0 1625 1671 2061 2414 3319 4126 4584 6095
Size: 1625 46 390 353 905 807 458 1511
Cuts at: 6095 7416
Size: 1321
Fragments arranged by size:
1625 1511 1321 905 807 458 390 353
46
BsrFI r'CCGG_y
Cuts at: 0 2867 3048 3175 4540 4571 4867 5451 7416
Size: 2867 181 127 1365 31 296 584 1965
Fragments arranged by size:
2867 1965 1365 584 296 181 127 31
BssSI C'ACGA_G
Cuts at: 0 889 1675 2284 2596 7416
Size: 889 786 609 312 4820
Fragments arranged by size:
4820 889 786 609 312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1282 2497 3507 5103 7416
Size: 1282 1215 1010 1596 2313
Fragments arranged by size:
2313 1596 1282 1215 1010
BstEII G'GTnAC_C
Cuts at: 0 2955 7416
Size: 2955 4461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1176 2239 7416
Size: 1176 1063 5177
Fragments arranged by size:
5177 1176 1063
BstYI r'GATC_y
Cuts at: 0 537 906 1034 1806 2124 2811 4740 4814
Size: 537 369 128 772 318 687 1929 74
Cuts at: 4814 5519 6659 6932 7134 7416
Size: 705 1140 273 202 282
Fragments arranged by size:
1929 1140 772 705 687 537 369 318
282 273 202 128 74
Bsu36I CC'TnA_GG
Cuts at: 0 103 662 5404 7416
Size: 103 559 4742 2012
Fragments arranged by size:
4742 2012 559 103
BtgI C'CryG_G
Cuts at: 0 2315 2546 2885 3571 3602 4818 6512 6812
Size: 2315 231 339 686 31 1216 1694 300
Cuts at: 6812 6860 7079 7416
Size: 48 219 337
Fragments arranged by size:
2315 1694 1216 686 339 337 300 231
219 48 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4518 5275 7416
Size: 4518 757 2141
Fragments arranged by size:
4518 2141 757
BtsI GCAGTG_nn'
Cuts at: 0 1276 1399 3127 3186 4878 5187 6107 6883
Size: 1276 123 1728 59 1692 309 920 776
Cuts at: 6883 7416
Size: 533
Fragments arranged by size:
1728 1692 1276 920 776 533 309 123
59
ClaI AT'CG_AT
Cuts at: 0 132 4163 7416
Size: 132 4031 3253
Fragments arranged by size:
4031 3253 132
DraIII CAC_nnn'GTG
Cuts at: 0 3603 7416
Size: 3603 3813
DrdI GACnn_nn'nnGTC
Cuts at: 0 5758 7321 7416
Size: 5758 1563 95
Fragments arranged by size:
5758 1563 95
EaeI y'GGCC_r
Cuts at: 0 849 1631 1902 2241 2339 3605 3662 4542
Size: 849 782 271 339 98 1266 57 880
Cuts at: 4542 5376 5678 6012 6206 6449 7149 7416
Size: 834 302 334 194 243 700 267
Fragments arranged by size:
1266 880 849 834 782 700 339 334
302 271 267 243 194 98 57
EagI C'GGCC_G
Cuts at: 0 5376 7416
Size: 5376 2040
EarI CTCTTCn'nnn_
Cuts at: 0 155 518 569 1417 2371 2935 3259 3743
Size: 155 363 51 848 954 564 324 484
Cuts at: 3743 4487 5594 5633 5847 6181 6280 6701 7416
Size: 744 1107 39 214 334 99 421 715
Fragments arranged by size:
1107 954 848 744 715 564 484 421
363 334 324 214 155 99 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5968 7416
Size: 5968 1448
EcoICRI GAG'CTC
Cuts at: 0 1752 1860 7284 7365 7416
Size: 1752 108 5424 81 51
Fragments arranged by size:
5424 1752 108 81 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 179 187 229 290 542 562 739
Size: 175 4 8 42 61 252 20 177
Cuts at: 739 919 1208 1378 2131 2677 2806 3127 3370
Size: 180 289 170 753 546 129 321 243
Cuts at: 3370 3439 3482 3490 3497 3981 4153 4309 5161
Size: 69 43 8 7 484 172 156 852
Cuts at: 5161 5584 5746 5902 5920 6259 6544 6555 6592
Size: 423 162 156 18 339 285 11 37
Cuts at: 6592 6943 7416
Size: 351 473
Fragments arranged by size:
852 753 546 484 473 423 351 339
321 289 285 252 243 180 177 175
172 170 162 156 156 129 69 61
43 42 37 20 18 11 8 8
7 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1336 2167 4291 7416
Size: 1336 831 2124 3125
Fragments arranged by size:
3125 2124 1336 831
EcoO109I rG'GnC_Cy
Cuts at: 0 63 723 2652 3414 3807 3840 4047 5034
Size: 63 660 1929 762 393 33 207 987
Cuts at: 5034 5145 5382 5856 6270 6519 6692 6716 6798
Size: 111 237 474 414 249 173 24 82
Cuts at: 6798 6822 6969 7242 7416
Size: 24 147 273 174
Fragments arranged by size:
1929 987 762 660 474 414 393 273
249 237 207 174 173 147 111 82
63 33 24 24
EcoRI G'AATT_C
Cuts at: 0 6979 7416
Size: 6979 437
EcoRV GAT'ATC
Cuts at: 0 2976 7416
Size: 2976 4440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1531 6142 7416
Size: 1531 4611 1274
Fragments arranged by size:
4611 1531 1274
FspI TGC'GCA
Cuts at: 0 1375 5444 6907 7416
Size: 1375 4069 1463 509
Fragments arranged by size:
4069 1463 1375 509
FspAI rTGC'GCAy
Cuts at: 0 6907 7416
Size: 6907 509
HaeII r_GCGC'y
Cuts at: 0 777 1354 1513 3642 3695 3894 4029 5235
Size: 777 577 159 2129 53 199 135 1206
Cuts at: 5235 7235 7416
Size: 2000 181
Fragments arranged by size:
2129 2000 1206 777 577 199 181 159
135 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2692 2724 2752 2784 2796 2828 3135 3167
Size: 2692 32 28 32 12 32 307 32
Cuts at: 3167 3457 3489 3776 3808 4039 4071 4308 4340
Size: 290 32 287 32 231 32 237 32
Cuts at: 4340 4491 4523 4615 4647 5314 5346 5789 5821
Size: 151 32 92 32 667 32 443 32
Cuts at: 5821 5908 5940 7030 7062 7416
Size: 87 32 1090 32 354
Fragments arranged by size:
2692 1090 667 443 354 307 290 287
237 231 151 92 87 32 32 32
32 32 32 32 32 32 32 32
32 32 32 28 12
HincII GTy'rAC
Cuts at: 0 1923 7416
Size: 1923 5493
HindIII A'AGCT_T
Cuts at: 0 241 1796 2017 2590 2633 2798 5738 6232
Size: 241 1555 221 573 43 165 2940 494
Cuts at: 6232 6380 7416
Size: 148 1036
Fragments arranged by size:
2940 1555 1036 573 494 241 221 165
148 43
MfeI C'AATT_G
Cuts at: 0 509 7416
Size: 509 6907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1838 3239 3336 4683 6515 7416
Size: 787 1051 1401 97 1347 1832 901
Fragments arranged by size:
1832 1401 1347 1051 901 787 97
MscI TGG'CCA
Cuts at: 0 1633 1904 2243 3607 5680 6208 7151 7416
Size: 1633 271 339 1364 2073 528 943 265
Fragments arranged by size:
2073 1633 1364 943 528 339 271 265
MslI CAynn'nnrTG
Cuts at: 0 1220 2249 3719 6907 7416
Size: 1220 1029 1470 3188 509
Fragments arranged by size:
3188 1470 1220 1029 509
MspA1I CmG'CkG
Cuts at: 0 714 1124 3660 4212 4344 4942 5529 5571
Size: 714 410 2536 552 132 598 587 42
Cuts at: 5571 5578 5592 6314 6447 6591 6888 6897 7416
Size: 7 14 722 133 144 297 9 519
Fragments arranged by size:
2536 722 714 598 587 552 519 410
297 144 133 132 42 14 9 7
NaeI GCC'GGC
Cuts at: 0 2869 3050 3177 4542 4573 4869 5453 7416
Size: 2869 181 127 1365 31 296 584 1963
Fragments arranged by size:
2869 1963 1365 584 296 181 127 31
NcoI C'CATG_G
Cuts at: 0 2315 2546 6512 6812 7079 7416
Size: 2315 231 3966 300 267 337
Fragments arranged by size:
3966 2315 337 300 267 231
NgoMIV G'CCGG_C
Cuts at: 0 2867 3048 3175 4540 4571 4867 5451 7416
Size: 2867 181 127 1365 31 296 584 1965
Fragments arranged by size:
2867 1965 1365 584 296 181 127 31
NheI G'CTAG_C
Cuts at: 0 1704 4098 7416
Size: 1704 2394 3318
Fragments arranged by size:
3318 2394 1704
NspI r_CATG'y
Cuts at: 0 550 1063 2161 3258 4801 6906 6912 7416
Size: 550 513 1098 1097 1543 2105 6 504
Fragments arranged by size:
2105 1543 1098 1097 550 513 504 6
PasI CC'CwG_GG
Cuts at: 0 3539 7062 7416
Size: 3539 3523 354
Fragments arranged by size:
3539 3523 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 450 540 3684 3740 6204 7416
Size: 450 90 3144 56 2464 1212
Fragments arranged by size:
3144 2464 1212 450 90 56
PfoI T'CCnGG_A
Cuts at: 0 44 2369 2710 3104 4043 4487 5309 5332
Size: 44 2325 341 394 939 444 822 23
Cuts at: 5332 5468 5968 6830 7121 7416
Size: 136 500 862 291 295
Fragments arranged by size:
2325 939 862 822 500 444 394 341
295 291 136 44 23
PpuMI rG'GwC_Cy
Cuts at: 0 63 2652 3414 3840 4047 5145 5382 6270
Size: 63 2589 762 426 207 1098 237 888
Cuts at: 6270 6692 6716 6798 7416
Size: 422 24 82 618
Fragments arranged by size:
2589 1098 888 762 618 426 422 237
207 82 63 24
PsiI TTA'TAA
Cuts at: 0 215 1802 7416
Size: 215 1587 5614
Fragments arranged by size:
5614 1587 215
PspOMI G'GGCC_C
Cuts at: 0 1697 7241 7416
Size: 1697 5544 175
Fragments arranged by size:
5544 1697 175
PstI C_TGCA'G
Cuts at: 0 440 737 1260 1409 1727 2574 2672 3932
Size: 440 297 523 149 318 847 98 1260
Cuts at: 3932 4025 4343 4514 5895 6077 6581 7416
Size: 93 318 171 1381 182 504 835
Fragments arranged by size:
1381 1260 847 835 523 504 440 318
318 297 182 171 149 98 93
PvuI CG_AT'CG
Cuts at: 0 3033 7416
Size: 3033 4383
PvuII CAG'CTG
Cuts at: 0 1124 3660 4212 4344 5571 5578 6314 6447
Size: 1124 2536 552 132 1227 7 736 133
Cuts at: 6447 6888 6897 7416
Size: 441 9 519
Fragments arranged by size:
2536 1227 1124 736 552 519 441 133
132 9 7
SacI G_AGCT'C
Cuts at: 0 1754 1862 7286 7367 7416
Size: 1754 108 5424 81 49
Fragments arranged by size:
5424 1754 108 81 49
SanDI GG'GwC_CC
Cuts at: 0 6716 7416
Size: 6716 700
SapI GCTCTTCn'nnn_
Cuts at: 0 155 518 1417 2371 7416
Size: 155 363 899 954 5045
Fragments arranged by size:
5045 954 899 363 155
SbfI CC_TGCA'GG
Cuts at: 0 3932 7416
Size: 3932 3484
ScaI AGT'ACT
Cuts at: 0 460 2683 7416
Size: 460 2223 4733
Fragments arranged by size:
4733 2223 460
SexAI A'CCwGG_T
Cuts at: 0 6502 7416
Size: 6502 914
SfcI C'TryA_G
Cuts at: 0 436 733 1256 1405 1723 2570 2668 3307
Size: 436 297 523 149 318 847 98 639
Cuts at: 3307 3652 3928 4021 4339 4510 5891 6073 6544
Size: 345 276 93 318 171 1381 182 471
Cuts at: 6544 6577 7416
Size: 33 839
Fragments arranged by size:
1381 847 839 639 523 471 436 345
318 318 297 276 182 171 149 98
93 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 6163 7416
Size: 6163 1253
SmaI CCC'GGG
Cuts at: 0 3094 4390 5584 7303 7416
Size: 3094 1296 1194 1719 113
Fragments arranged by size:
3094 1719 1296 1194 113
SmlI C'TyrA_G
Cuts at: 0 1516 1753 2305 7416
Size: 1516 237 552 5111
Fragments arranged by size:
5111 1516 552 237
SphI G_CATG'C
Cuts at: 0 4801 6906 6912 7416
Size: 4801 2105 6 504
Fragments arranged by size:
4801 2105 504 6
StuI AGG'CCT
Cuts at: 0 3958 4297 4396 4516 6079 7416
Size: 3958 339 99 120 1563 1337
Fragments arranged by size:
3958 1563 1337 339 120 99
StyI C'CwwG_G
Cuts at: 0 1043 1493 1889 1934 2315 2546 3873 4103
Size: 1043 450 396 45 381 231 1327 230
Cuts at: 4103 4553 5606 6152 6365 6512 6812 7079 7274
Size: 450 1053 546 213 147 300 267 195
Cuts at: 7274 7416
Size: 142
Fragments arranged by size:
1327 1053 1043 546 450 450 396 381
300 267 231 230 213 195 147 142
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 1116 2210 3226 3994 7416
Size: 86 1030 1094 1016 768 3422
Fragments arranged by size:
3422 1094 1030 1016 768 86
TatI w'GTAC_w
Cuts at: 0 458 1830 2681 3702 6842 7416
Size: 458 1372 851 1021 3140 574
Fragments arranged by size:
3140 1372 1021 851 574 458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6133 6165 7119 7151 7416
Size: 6133 32 954 32 265
Fragments arranged by size:
6133 954 265 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 928 7416
Size: 928 6488
XbaI T'CTAG_A
Cuts at: 0 918 7416
Size: 918 6498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 1941 6519 7416
Size: 300 1641 4578 897
Fragments arranged by size:
4578 1641 897 300
XmaI C'CCGG_G
Cuts at: 0 3092 4388 5582 7301 7416
Size: 3092 1296 1194 1719 115
Fragments arranged by size:
3092 1719 1296 1194 115
XmnI GAAnn'nnTTC
Cuts at: 0 188 578 1511 7416
Size: 188 390 933 5905
Fragments arranged by size:
5905 933 390 188
ZraI GAC'GTC
Cuts at: 0 4603 7416
Size: 4603 2813
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PasI PflMI PfoI PpuMI
PsiI PspOMI PstI PvuI PvuII SacI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AscI AseI AsiSI AvrII
BaeI BanI BbeI BfrBI BspEI BsrGI BssHII BstBI
BstZ17I CspCI DraI FseI HpaI KasI KpnI MluI
NarI NdeI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PspXI PsrI RsrII SacII SalI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XhoI