Restriction map for
pF1KB5528
(Linear) (Six-base) MAPSORT of: KIBB5528.seq Check: 3801 from: 1 to: 4269
KIBB5528.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1492 4269
Size: 1492 2777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1090 3367 3505 3819 4269
Size: 337 753 2277 138 314 450
Fragments arranged by size:
2277 753 450 337 314 138
AflII C'TTAA_G
Cuts at: 0 508 4269
Size: 508 3761
AleI CACnn'nnGTG
Cuts at: 0 1778 4269
Size: 1778 2491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 695 3709 4154 4269
Size: 695 3014 445 115
Fragments arranged by size:
3014 695 445 115
AlwNI CAG_nnn'CTG
Cuts at: 0 3531 4269
Size: 3531 738
ApaLI G'TGCA_C
Cuts at: 0 1507 2304 4174 4269
Size: 1507 797 1870 95
Fragments arranged by size:
1870 1507 797 95
ApoI r'AATT_y
Cuts at: 0 513 846 2076 2799 3093 4101 4269
Size: 513 333 1230 723 294 1008 168
Fragments arranged by size:
1230 1008 723 513 333 294 168
BamHI G'GATC_C
Cuts at: 0 24 4269
Size: 24 4245
BanII G_rGCy'C
Cuts at: 0 1382 3269 3646 4269
Size: 1382 1887 377 623
Fragments arranged by size:
1887 1382 623 377
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 632 2823 4269
Size: 497 135 2191 1446
Fragments arranged by size:
2191 1446 497 135
BbvCI CC'TCA_GC
Cuts at: 0 2087 4269
Size: 2087 2182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2524 2558 3986 4020 4269
Size: 2524 34 1428 34 249
Fragments arranged by size:
2524 1428 249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2010 4269
Size: 2010 2259
BclI T'GATC_A
Cuts at: 0 4254 4269
Size: 4254 15
BfrBI ATG'CAT
Cuts at: 0 2019 4269
Size: 2019 2250
BlpI GC'TnA_GC
Cuts at: 0 146 4269
Size: 146 4123
Bme1580I G_kGCm'C
Cuts at: 0 1511 1575 2308 4178 4269
Size: 1511 64 733 1870 91
Fragments arranged by size:
1870 1511 733 91 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 985 1547 2126 2766 2869 3028 4269
Size: 985 562 579 640 103 159 1241
Fragments arranged by size:
1241 985 640 579 562 159 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1792 4269
Size: 1792 2477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1991 2012 2402 2588 4269
Size: 56 1935 21 390 186 1681
Fragments arranged by size:
1935 1681 390 186 56 21
Bpu10I CC'TnA_GC
Cuts at: 0 2087 4269
Size: 2087 2182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2313 2580 2682 4269
Size: 2313 267 102 1587
Fragments arranged by size:
2313 1587 267 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 2907 3474 4269
Size: 550 2357 567 795
Fragments arranged by size:
2357 795 567 550
BsaWI w'CCGG_w
Cuts at: 0 2045 4269
Size: 2045 2224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1398 1428 2724 2754 4269
Size: 1398 30 1296 30 1515
Fragments arranged by size:
1515 1398 1296 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1439 1968 1971 2009 2840 3899 4269
Size: 1439 529 3 38 831 1059 370
Fragments arranged by size:
1439 1059 831 529 370 38 3
BseYI C'CCAG_C
Cuts at: 0 3345 3865 4127 4269
Size: 3345 520 262 142
Fragments arranged by size:
3345 520 262 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 2554 2840 3154 4269
Size: 1016 1538 286 314 1115
Fragments arranged by size:
1538 1115 1016 314 286
BsiHKAI G_wGCw'C
Cuts at: 0 1511 2308 3646 4178 4269
Size: 1511 797 1338 532 91
Fragments arranged by size:
1511 1338 797 532 91
BsmI GAATG_Cn'
Cuts at: 0 3230 3461 3518 4269
Size: 3230 231 57 751
Fragments arranged by size:
3230 751 231 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 102 383 4269
Size: 102 281 3886
Fragments arranged by size:
3886 281 102
Bsp1286I G_dGCh'C
Cuts at: 0 1382 1511 1575 2308 3269 3646 4178 4269
Size: 1382 129 64 733 961 377 532 91
Fragments arranged by size:
1382 961 733 532 377 129 91 64
BspHI T'CATG_A
Cuts at: 0 1439 3476 4269
Size: 1439 2037 793
Fragments arranged by size:
2037 1439 793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 440 1492 2228 2862 4269
Size: 97 343 1052 736 634 1407
Fragments arranged by size:
1407 1052 736 634 343 97
BsrDI GCAATG_nn'
Cuts at: 0 2878 4004 4269
Size: 2878 1126 265
Fragments arranged by size:
2878 1126 265
BsrFI r'CCGG_y
Cuts at: 0 2779 4269
Size: 2779 1490
BssSI C'ACGA_G
Cuts at: 0 695 3464 4269
Size: 695 2769 805
Fragments arranged by size:
2769 805 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2061 2940 3531 4269
Size: 2061 879 591 738
Fragments arranged by size:
2061 879 738 591
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2976 3204 4269
Size: 2976 228 1065
Fragments arranged by size:
2976 1065 228
BstYI r'GATC_y
Cuts at: 0 24 856 2377 3960 4269
Size: 24 832 1521 1583 309
Fragments arranged by size:
1583 1521 832 309 24
Bsu36I CC'TnA_GG
Cuts at: 0 336 2748 4269
Size: 336 2412 1521
Fragments arranged by size:
2412 1521 336
BtgI C'CryG_G
Cuts at: 0 1517 2810 2969 3047 4269
Size: 1517 1293 159 78 1222
Fragments arranged by size:
1517 1293 1222 159 78
BtsI GCAGTG_nn'
Cuts at: 0 99 679 1809 2570 4166 4197 4269
Size: 99 580 1130 761 1596 31 72
Fragments arranged by size:
1596 1130 761 580 99 72 31
ClaI AT'CG_AT
Cuts at: 0 999 4269
Size: 999 3270
DraI TTT'AAA
Cuts at: 0 215 4269
Size: 215 4054
DraIII CAC_nnn'GTG
Cuts at: 0 2863 4269
Size: 2863 1406
DrdI GACnn_nn'nnGTC
Cuts at: 0 236 4269
Size: 236 4033
EaeI y'GGCC_r
Cuts at: 0 32 975 2994 4090 4106 4269
Size: 32 943 2019 1096 16 163
Fragments arranged by size:
2019 1096 943 163 32 16
EarI CTCTTCn'nnn_
Cuts at: 0 1057 2366 2891 3089 3182 4269
Size: 1057 1309 525 198 93 1087
Fragments arranged by size:
1309 1087 1057 525 198 93
EcoICRI GAG'CTC
Cuts at: 0 3644 4269
Size: 3644 625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 337 1090 1991 2012 2402 2588 3367
Size: 56 281 753 901 21 390 186 779
Cuts at: 3367 3505 3819 4269
Size: 138 314 450
Fragments arranged by size:
901 779 753 450 390 314 281 186
138 56 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 864 1334 1499 2747 3449 4269
Size: 864 470 165 1248 702 820
Fragments arranged by size:
1248 864 820 702 470 165
EcoO109I rG'GnC_Cy
Cuts at: 0 450 1244 1633 1831 3355 3889 4018 4249
Size: 450 794 389 198 1524 534 129 231
Cuts at: 4249 4269
Size: 20
Fragments arranged by size:
1524 794 534 450 389 231 198 129
20
EcoRI G'AATT_C
Cuts at: 0 2076 2799 3093 4269
Size: 2076 723 294 1176
Fragments arranged by size:
2076 1176 723 294
EcoRV GAT'ATC
Cuts at: 0 155 4269
Size: 155 4114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 985 1850 4269
Size: 985 865 2419
Fragments arranged by size:
2419 985 865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 316 348 712 744 916 948
Size: 139 32 145 32 364 32 172 32
Cuts at: 948 1398 1430 1434 1466 2236 2268 2664 2696
Size: 450 32 4 32 770 32 396 32
Cuts at: 2696 2718 2724 2750 2756 3458 3490 4269
Size: 22 6 26 6 702 32 779
Fragments arranged by size:
779 770 702 450 396 364 172 145
139 32 32 32 32 32 32 32
32 32 26 22 6 6 4
HincII GTy'rAC
Cuts at: 0 459 4269
Size: 459 3810
HindIII A'AGCT_T
Cuts at: 0 962 4269
Size: 962 3307
HpaI GTT'AAC
Cuts at: 0 459 4269
Size: 459 3810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1128 3149 3735 4269
Size: 1128 2021 586 534
Fragments arranged by size:
2021 1128 586 534
MscI TGG'CCA
Cuts at: 0 34 977 2996 4092 4108 4269
Size: 34 943 2019 1096 16 161
Fragments arranged by size:
2019 1096 943 161 34 16
MslI CAynn'nnrTG
Cuts at: 0 1204 1778 2974 4181 4228 4243 4269
Size: 1204 574 1196 1207 47 15 26
Fragments arranged by size:
1207 1204 1196 574 47 26 15
MspA1I CmG'CkG
Cuts at: 0 2086 3168 4269
Size: 2086 1082 1101
Fragments arranged by size:
2086 1101 1082
NaeI GCC'GGC
Cuts at: 0 2781 4269
Size: 2781 1488
NcoI C'CATG_G
Cuts at: 0 1517 2810 2969 3047 4269
Size: 1517 1293 159 78 1222
Fragments arranged by size:
1517 1293 1222 159 78
NgoMIV G'CCGG_C
Cuts at: 0 2779 4269
Size: 2779 1490
NsiI A_TGCA'T
Cuts at: 0 2021 4269
Size: 2021 2248
NspI r_CATG'y
Cuts at: 0 1344 3042 4242 4269
Size: 1344 1698 1200 27
Fragments arranged by size:
1698 1344 1200 27
PasI CC'CwG_GG
Cuts at: 0 1330 3648 4269
Size: 1330 2318 621
Fragments arranged by size:
2318 1330 621
PflMI CCAn_nnn'nTGG
Cuts at: 0 3940 3970 4269
Size: 3940 30 299
Fragments arranged by size:
3940 299 30
PfoI T'CCnGG_A
Cuts at: 0 72 544 4269
Size: 72 472 3725
Fragments arranged by size:
3725 472 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2490 2522 4269
Size: 2490 32 1747
Fragments arranged by size:
2490 1747 32
PpuMI rG'GwC_Cy
Cuts at: 0 450 1831 3889 4018 4249 4269
Size: 450 1381 2058 129 231 20
Fragments arranged by size:
2058 1381 450 231 129 20
PshAI GACnn'nnGTC
Cuts at: 0 1895 4269
Size: 1895 2374
PsiI TTA'TAA
Cuts at: 0 2834 4269
Size: 2834 1435
PstI C_TGCA'G
Cuts at: 0 94 1868 2069 2109 3536 4161 4269
Size: 94 1774 201 40 1427 625 108
Fragments arranged by size:
1774 1427 625 201 108 94 40
PvuII CAG'CTG
Cuts at: 0 2086 3168 4269
Size: 2086 1082 1101
Fragments arranged by size:
2086 1101 1082
SacI G_AGCT'C
Cuts at: 0 3646 4269
Size: 3646 623
SanDI GG'GwC_CC
Cuts at: 0 4018 4269
Size: 4018 251
SexAI A'CCwGG_T
Cuts at: 0 452 2904 4269
Size: 452 2452 1365
Fragments arranged by size:
2452 1365 452
SfcI C'TryA_G
Cuts at: 0 90 305 527 761 1022 1864 2065 2105
Size: 90 215 222 234 261 842 201 40
Cuts at: 2105 2263 3532 4157 4269
Size: 158 1269 625 112
Fragments arranged by size:
1269 842 625 261 234 222 215 201
158 112 90 40
SmlI C'TyrA_G
Cuts at: 0 508 2328 2595 2697 4269
Size: 508 1820 267 102 1572
Fragments arranged by size:
1820 1572 508 267 102
SpeI A'CTAG_T
Cuts at: 0 2422 4269
Size: 2422 1847
SphI G_CATG'C
Cuts at: 0 1344 4242 4269
Size: 1344 2898 27
Fragments arranged by size:
2898 1344 27
SspI AAT'ATT
Cuts at: 0 1665 4269
Size: 1665 2604
StuI AGG'CCT
Cuts at: 0 870 2955 3081 3203 3621 3832 4269
Size: 870 2085 126 122 418 211 437
Fragments arranged by size:
2085 870 437 418 211 126 122
StyI C'CwwG_G
Cuts at: 0 1218 1517 2810 2969 3047 3285 3450 4269
Size: 1218 299 1293 159 78 238 165 819
Fragments arranged by size:
1293 1218 819 299 238 165 159 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2644 2695 2851 2879 3476 4269
Size: 2644 51 156 28 597 793
Fragments arranged by size:
2644 793 597 156 51 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 1537 1569 4269
Size: 1010 32 495 32 2700
Fragments arranged by size:
2700 1010 495 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 80 4269
Size: 80 4189
XbaI T'CTAG_A
Cuts at: 0 2397 4269
Size: 2397 1872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1707 2856 4269
Size: 1707 1149 1413
Fragments arranged by size:
1707 1413 1149
XmnI GAAnn'nnTTC
Cuts at: 0 2502 4269
Size: 2502 1767
Enzymes that do cut:
AarI AcuI AflII AleI AlfI AlwNI ApaLI ApoI
BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI ClaI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PsiI PstI PvuII SacI
SanDI SexAI SfcI SmlI SpeI SphI SspI StuI
StyI TaqII TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BglI BglII BmgBI BmtI BsaAI BsaBI
BsaHI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI CspCI EagI EciI FseI FspI
FspAI HaeII KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SwaI TatI XhoI XmaI ZraI