(Linear) (Six-base) MAPSORT of: KIBB5530.seq  Check: 8771  from: 1  to: 360



KIBB5530.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AvaI C'yCGr_G

Cuts at:      0     112     360 
   Size:        112     248
 
BanI G'GyrC_C

Cuts at:      0     253     360 
   Size:        253     107
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     272     360 
   Size:        272      88
 
BsaBI GATnn'nnATC

Cuts at:      0     355     360 
   Size:        355       5
 
BsaHI Gr'CG_yC

Cuts at:      0      77     360 
   Size:         77     283
 
BseYI C'CCAG_C

Cuts at:      0      31     360 
   Size:         31     329
 
BspHI T'CATG_A

Cuts at:      0      93     360 
   Size:         93     267
 
BsrFI r'CCGG_y

Cuts at:      0      13     360 
   Size:         13     347
 
BtgI C'CryG_G

Cuts at:      0      62     197     360 
   Size:         62     135     163

  Fragments arranged by size: 

                 163     135      62
 
EaeI y'GGCC_r

Cuts at:      0      21      65     360 
   Size:         21      44     295

  Fragments arranged by size: 

                 295      44      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     360 
   Size:        130     230
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     360 
   Size:        158     202
 
MscI TGG'CCA

Cuts at:      0      67     360 
   Size:         67     293
 
MspA1I CmG'CkG

Cuts at:      0      20     360 
   Size:         20     340
 
NaeI GCC'GGC

Cuts at:      0      15     360 
   Size:         15     345
 
NcoI C'CATG_G

Cuts at:      0     197     360 
   Size:        197     163
 
NgoMIV G'CCGG_C

Cuts at:      0      13     360 
   Size:         13     347
 
NspI r_CATG'y

Cuts at:      0     325     360 
   Size:        325      35
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130     360 
   Size:        130     230
 
SmaI CCC'GGG

Cuts at:      0     114     360 
   Size:        114     246
 
StyI C'CwwG_G

Cuts at:      0     197     360 
   Size:        197     163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     360 
   Size:         60     300
 
XmaI C'CCGG_G

Cuts at:      0     112     360 
   Size:        112     248
 

 Enzymes that do cut:

     AvaI     BanI     BbsI    BsaBI    BsaHI    BseYI    BspHI    BsrFI
     BtgI     EaeI     EarI     MmeI     MscI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     SapI     SmaI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI