Restriction map for
pF1KB5539
(Linear) (Six-base) MAPSORT of: KIBB5539.seq Check: 7392 from: 1 to: 1683
KIBB5539.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 4 929 1683
Size: 4 925 754
Fragments arranged by size:
925 754 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 882 1067 1216 1508 1683
Size: 866 16 185 149 292 175
Fragments arranged by size:
866 292 185 175 149 16
AflIII A'CryG_T
Cuts at: 0 1520 1683
Size: 1520 163
AlwNI CAG_nnn'CTG
Cuts at: 0 354 376 1636 1683
Size: 354 22 1260 47
Fragments arranged by size:
1260 354 47 22
AvaI C'yCGr_G
Cuts at: 0 1118 1683
Size: 1118 565
BamHI G'GATC_C
Cuts at: 0 65 965 1683
Size: 65 900 718
Fragments arranged by size:
900 718 65
BanI G'GyrC_C
Cuts at: 0 1129 1683
Size: 1129 554
BanII G_rGCy'C
Cuts at: 0 1119 1212 1591 1683
Size: 1119 93 379 92
Fragments arranged by size:
1119 379 93 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 674 1055 1496 1683
Size: 674 381 441 187
Fragments arranged by size:
674 441 381 187
BbvCI CC'TCA_GC
Cuts at: 0 1621 1683
Size: 1621 62
BciVI GTATCCnnnnn_n'
Cuts at: 0 1462 1683
Size: 1462 221
BglII A'GATC_T
Cuts at: 0 400 1361 1403 1683
Size: 400 961 42 280
Fragments arranged by size:
961 400 280 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 881 1391 1683
Size: 364 517 510 292
Fragments arranged by size:
517 510 364 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 629 1683
Size: 629 1054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 592 713 1613 1683
Size: 482 110 121 900 70
Fragments arranged by size:
900 482 121 110 70
Bpu10I CC'TnA_GC
Cuts at: 0 1228 1621 1683
Size: 1228 393 62
Fragments arranged by size:
1228 393 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1570 1683
Size: 1570 113
BsaBI GATnn'nnATC
Cuts at: 0 970 1683
Size: 970 713
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1061 1091 1683
Size: 1061 30 592
Fragments arranged by size:
1061 592 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 746 785 1683
Size: 746 39 898
Fragments arranged by size:
898 746 39
BseYI C'CCAG_C
Cuts at: 0 69 1022 1683
Size: 69 953 661
Fragments arranged by size:
953 661 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1683
Size: 725 958
BsiHKAI G_wGCw'C
Cuts at: 0 252 950 1591 1683
Size: 252 698 641 92
Fragments arranged by size:
698 641 252 92
Bsp1286I G_dGCh'C
Cuts at: 0 252 950 1119 1212 1591 1683
Size: 252 698 169 93 379 92
Fragments arranged by size:
698 379 252 169 93 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 1683
Size: 95 1588
BsrBI CCG'CTC
Cuts at: 0 129 1683
Size: 129 1554
BsrDI GCAATG_nn'
Cuts at: 0 1431 1683
Size: 1431 252
BsrFI r'CCGG_y
Cuts at: 0 206 1683
Size: 206 1477
BstAPI GCAn_nnn'nTGC
Cuts at: 0 85 1270 1683
Size: 85 1185 413
Fragments arranged by size:
1185 413 85
BstEII G'GTnAC_C
Cuts at: 0 1282 1683
Size: 1282 401
BstXI CCAn_nnnn'nTGG
Cuts at: 0 707 976 1683
Size: 707 269 707
Fragments arranged by size:
707 707 269
BstYI r'GATC_y
Cuts at: 0 65 350 400 965 1361 1385 1403 1683
Size: 65 285 50 565 396 24 18 280
Fragments arranged by size:
565 396 285 280 65 50 24 18
Bsu36I CC'TnA_GG
Cuts at: 0 1204 1278 1683
Size: 1204 74 405
Fragments arranged by size:
1204 405 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 296 1683
Size: 296 1387
DrdI GACnn_nn'nnGTC
Cuts at: 0 30 1683
Size: 30 1653
EaeI y'GGCC_r
Cuts at: 0 204 1396 1535 1683
Size: 204 1192 139 148
Fragments arranged by size:
1192 204 148 139
EarI CTCTTCn'nnn_
Cuts at: 0 119 598 657 1289 1683
Size: 119 479 59 632 394
Fragments arranged by size:
632 479 394 119 59
EcoICRI GAG'CTC
Cuts at: 0 1589 1683
Size: 1589 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 592 713 866 882 1067 1216 1508
Size: 482 110 121 153 16 185 149 292
Cuts at: 1508 1613 1683
Size: 105 70
Fragments arranged by size:
482 292 185 153 149 121 110 105
70 16
EcoO109I rG'GnC_Cy
Cuts at: 0 633 1122 1200 1683
Size: 633 489 78 483
Fragments arranged by size:
633 489 483 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1425 1683
Size: 1425 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 472 504 1106 1138 1683
Size: 234 32 206 32 602 32 545
Fragments arranged by size:
602 545 234 206 32 32 32
HincII GTy'rAC
Cuts at: 0 1167 1683
Size: 1167 516
HindIII A'AGCT_T
Cuts at: 0 1003 1301 1683
Size: 1003 298 382
Fragments arranged by size:
1003 382 298
MfeI C'AATT_G
Cuts at: 0 994 1475 1683
Size: 994 481 208
Fragments arranged by size:
994 481 208
MscI TGG'CCA
Cuts at: 0 1398 1537 1683
Size: 1398 139 146
Fragments arranged by size:
1398 146 139
MslI CAynn'nnrTG
Cuts at: 0 784 911 1683
Size: 784 127 772
Fragments arranged by size:
784 772 127
MspA1I CmG'CkG
Cuts at: 0 231 1683
Size: 231 1452
NaeI GCC'GGC
Cuts at: 0 208 1683
Size: 208 1475
NgoMIV G'CCGG_C
Cuts at: 0 206 1683
Size: 206 1477
NspI r_CATG'y
Cuts at: 0 1066 1524 1683
Size: 1066 458 159
Fragments arranged by size:
1066 458 159
PasI CC'CwG_GG
Cuts at: 0 54 1683
Size: 54 1629
PciI A'CATG_T
Cuts at: 0 1520 1683
Size: 1520 163
PpuMI rG'GwC_Cy
Cuts at: 0 633 1122 1200 1683
Size: 633 489 78 483
Fragments arranged by size:
633 489 483 78
PsiI TTA'TAA
Cuts at: 0 520 1683
Size: 520 1163
PstI C_TGCA'G
Cuts at: 0 624 1683
Size: 624 1059
PvuII CAG'CTG
Cuts at: 0 231 1683
Size: 231 1452
SacI G_AGCT'C
Cuts at: 0 1591 1683
Size: 1591 92
SanDI GG'GwC_CC
Cuts at: 0 633 1683
Size: 633 1050
SapI GCTCTTCn'nnn_
Cuts at: 0 119 1683
Size: 119 1564
SfcI C'TryA_G
Cuts at: 0 620 1683
Size: 620 1063
SmaI CCC'GGG
Cuts at: 0 1120 1683
Size: 1120 563
SspI AAT'ATT
Cuts at: 0 222 1683
Size: 222 1461
StyI C'CwwG_G
Cuts at: 0 60 1133 1683
Size: 60 1073 550
Fragments arranged by size:
1073 550 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 777 1683
Size: 777 906
TatI w'GTAC_w
Cuts at: 0 1458 1683
Size: 1458 225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 726 1683
Size: 726 957
XmaI C'CCGG_G
Cuts at: 0 1118 1683
Size: 1118 565
XmnI GAAnn'nnTTC
Cuts at: 0 596 1683
Size: 596 1087
Enzymes that do cut:
AccI AcuI AflIII AlwNI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BglII BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgZI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII HindIII MfeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PciI PpuMI PsiI PstI
PvuII SacI SanDI SapI SfcI SmaI SspI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmtI BpuEI BsaAI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstBI BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XhoI ZraI