Restriction map for
pF1KB5542
(Linear) (Six-base) MAPSORT of: KIBB5542.seq Check: 9286 from: 1 to: 1074
KIBB5542.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AflIII A'CryG_T
Cuts at: 0 624 833 1074
Size: 624 209 241
Fragments arranged by size:
624 241 209
AleI CACnn'nnGTG
Cuts at: 0 94 553 1074
Size: 94 459 521
Fragments arranged by size:
521 459 94
ApaI G_GGCC'C
Cuts at: 0 377 678 1074
Size: 377 301 396
Fragments arranged by size:
396 377 301
AvaI C'yCGr_G
Cuts at: 0 862 1074
Size: 862 212
BanI G'GyrC_C
Cuts at: 0 198 207 807 1074
Size: 198 9 600 267
Fragments arranged by size:
600 267 198 9
BanII G_rGCy'C
Cuts at: 0 67 122 377 614 678 752 869 1000
Size: 67 55 255 237 64 74 117 131
Cuts at: 1000 1074
Size: 74
Fragments arranged by size:
255 237 131 117 74 74 67 64
55
BbeI G_GCGC'C
Cuts at: 0 211 1074
Size: 211 863
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 1074
Size: 551 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 180 214 1074
Size: 180 34 860
Fragments arranged by size:
860 180 34
BfrBI ATG'CAT
Cuts at: 0 357 1074
Size: 357 717
BglI GCCn_nnn'nGGC
Cuts at: 0 381 517 1074
Size: 381 136 557
Fragments arranged by size:
557 381 136
BglII A'GATC_T
Cuts at: 0 485 1074
Size: 485 589
Bme1580I G_kGCm'C
Cuts at: 0 377 678 1050 1074
Size: 377 301 372 24
Fragments arranged by size:
377 372 301 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 1074
Size: 150 924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 628 836 980 1074
Size: 598 30 208 144 94
Fragments arranged by size:
598 208 144 94 30
Bpu10I CC'TnA_GC
Cuts at: 0 999 1074
Size: 999 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 751 960 1074
Size: 341 410 209 114
Fragments arranged by size:
410 341 209 114
BsaAI yAC'GTr
Cuts at: 0 144 627 836 1074
Size: 144 483 209 238
Fragments arranged by size:
483 238 209 144
BsaHI Gr'CG_yC
Cuts at: 0 179 208 1074
Size: 179 29 866
Fragments arranged by size:
866 179 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 953 983 1029 1059 1074
Size: 439 30 484 30 46 30 15
Fragments arranged by size:
484 439 46 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 835 1074
Size: 16 819 239
Fragments arranged by size:
819 239 16
BseYI C'CCAG_C
Cuts at: 0 369 671 896 1074
Size: 369 302 225 178
Fragments arranged by size:
369 302 225 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1074
Size: 865 209
BsiEI CG_ry'CG
Cuts at: 0 111 1074
Size: 111 963
BsiHKAI G_wGCw'C
Cuts at: 0 527 752 1000 1074
Size: 527 225 248 74
Fragments arranged by size:
527 248 225 74
BsmBI CGTCTCn'nnnn_
Cuts at: 0 586 1074
Size: 586 488
Bsp1286I G_dGCh'C
Cuts at: 0 67 122 377 527 614 678 752 869
Size: 67 55 255 150 87 64 74 117
Cuts at: 869 1000 1050 1074
Size: 131 50 24
Fragments arranged by size:
255 150 131 117 87 74 67 64
55 50 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 458 1074
Size: 302 156 616
Fragments arranged by size:
616 302 156
BsrDI GCAATG_nn'
Cuts at: 0 923 1074
Size: 923 151
BsrFI r'CCGG_y
Cuts at: 0 888 1074
Size: 888 186
BssSI C'ACGA_G
Cuts at: 0 751 1074
Size: 751 323
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 1074
Size: 354 720
BstYI r'GATC_y
Cuts at: 0 485 946 1074
Size: 485 461 128
Fragments arranged by size:
485 461 128
Bsu36I CC'TnA_GG
Cuts at: 0 664 1074
Size: 664 410
BtgI C'CryG_G
Cuts at: 0 111 202 211 393 464 1074
Size: 111 91 9 182 71 610
Fragments arranged by size:
610 182 111 91 71 9
BtsI GCAGTG_nn'
Cuts at: 0 355 1074
Size: 355 719
EaeI y'GGCC_r
Cuts at: 0 108 1074
Size: 108 966
EagI C'GGCC_G
Cuts at: 0 108 1074
Size: 108 966
EarI CTCTTCn'nnn_
Cuts at: 0 988 1074
Size: 988 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 535 1074
Size: 535 539
EcoICRI GAG'CTC
Cuts at: 0 750 998 1074
Size: 750 248 76
Fragments arranged by size:
750 248 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 628 836 980 1074
Size: 598 30 208 144 94
Fragments arranged by size:
598 208 144 94 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 442 580 1074
Size: 31 411 138 494
Fragments arranged by size:
494 411 138 31
EcoO109I rG'GnC_Cy
Cuts at: 0 36 373 447 675 1074
Size: 36 337 74 228 399
Fragments arranged by size:
399 337 228 74 36
HaeII r_GCGC'y
Cuts at: 0 211 263 387 1074
Size: 211 52 124 687
Fragments arranged by size:
687 211 124 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 439 471 484 516 938 970
Size: 319 32 88 32 13 32 422 32
Cuts at: 970 1074
Size: 104
Fragments arranged by size:
422 319 104 88 32 32 32 32
13
KasI G'GCGC_C
Cuts at: 0 207 1074
Size: 207 867
MslI CAynn'nnrTG
Cuts at: 0 94 133 553 1074
Size: 94 39 420 521
Fragments arranged by size:
521 420 94 39
MspA1I CmG'CkG
Cuts at: 0 113 204 395 466 799 1074
Size: 113 91 191 71 333 275
Fragments arranged by size:
333 275 191 113 91 71
NaeI GCC'GGC
Cuts at: 0 890 1074
Size: 890 184
NarI GG'CG_CC
Cuts at: 0 208 1074
Size: 208 866
NgoMIV G'CCGG_C
Cuts at: 0 888 1074
Size: 888 186
NsiI A_TGCA'T
Cuts at: 0 359 1074
Size: 359 715
NspI r_CATG'y
Cuts at: 0 554 1074
Size: 554 520
PasI CC'CwG_GG
Cuts at: 0 678 1050 1074
Size: 678 372 24
Fragments arranged by size:
678 372 24
PfoI T'CCnGG_A
Cuts at: 0 457 1074
Size: 457 617
PmlI CAC'GTG
Cuts at: 0 627 836 1074
Size: 627 209 238
Fragments arranged by size:
627 238 209
PpuMI rG'GwC_Cy
Cuts at: 0 447 1074
Size: 447 627
PspOMI G'GGCC_C
Cuts at: 0 373 674 1074
Size: 373 301 400
Fragments arranged by size:
400 373 301
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 391 423 1074
Size: 391 32 651
Fragments arranged by size:
651 391 32
PstI C_TGCA'G
Cuts at: 0 350 771 1074
Size: 350 421 303
Fragments arranged by size:
421 350 303
PvuII CAG'CTG
Cuts at: 0 799 1074
Size: 799 275
SacI G_AGCT'C
Cuts at: 0 752 1000 1074
Size: 752 248 74
Fragments arranged by size:
752 248 74
SacII CC_GC'GG
Cuts at: 0 114 205 396 467 1074
Size: 114 91 191 71 607
Fragments arranged by size:
607 191 114 91 71
SapI GCTCTTCn'nnn_
Cuts at: 0 988 1074
Size: 988 86
SbfI CC_TGCA'GG
Cuts at: 0 771 1074
Size: 771 303
SfcI C'TryA_G
Cuts at: 0 346 767 1074
Size: 346 421 307
Fragments arranged by size:
421 346 307
SfoI GGC'GCC
Cuts at: 0 209 1074
Size: 209 865
StuI AGG'CCT
Cuts at: 0 765 1074
Size: 765 309
StyI C'CwwG_G
Cuts at: 0 942 1074
Size: 942 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 1074
Size: 169 905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 1029 1061 1074
Size: 266 32 731 32 13
Fragments arranged by size:
731 266 32 32 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 1074
Size: 912 162
XmnI GAAnn'nnTTC
Cuts at: 0 82 1074
Size: 82 992
Enzymes that do cut:
AflIII AleI ApaI AvaI BanI BanII BbeI BbsI
BcgI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PfoI
PmlI PpuMI PspOMI PsrI PstI PvuII SacI SacII
SapI SbfI SfcI SfoI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BlpI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SalI SanDI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI