(Linear) (Six-base) MAPSORT of: KIBB5543.seq  Check: 3639  from: 1  to: 1038



KIBB5543.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     769    1038 
   Size:        769     269
 
ApoI r'AATT_y

Cuts at:      0     682     895    1038 
   Size:        682     213     143

  Fragments arranged by size: 

                 682     213     143
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     437    1038 
   Size:        437     601
 
BclI T'GATC_A

Cuts at:      0     269    1038 
   Size:        269     769
 
BglII A'GATC_T

Cuts at:      0     309    1038 
   Size:        309     729
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     779    1038 
   Size:        779     259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     167     743     749    1038 
   Size:         54     113     576       6     289

  Fragments arranged by size: 

                 576     289     113      54       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437    1038 
   Size:        437     601
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     848    1038 
   Size:        848     190
 
BsmI GAATG_Cn'

Cuts at:      0     117    1038 
   Size:        117     921
 
BspHI T'CATG_A

Cuts at:      0     609    1038 
   Size:        609     429
 
BsrBI CCG'CTC

Cuts at:      0     176    1038 
   Size:        176     862
 
BssSI C'ACGA_G

Cuts at:      0      31    1038 
   Size:         31    1007
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273     500    1038 
   Size:        273     227     538

  Fragments arranged by size: 

                 538     273     227
 
BstYI r'GATC_y

Cuts at:      0     309     519     840    1038 
   Size:        309     210     321     198

  Fragments arranged by size: 

                 321     309     210     198
 
BtsI GCAGTG_nn'

Cuts at:      0     173     208    1038 
   Size:        173      35     830

  Fragments arranged by size: 

                 830     173      35
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     384     419    1038 
   Size:        384      35     619

  Fragments arranged by size: 

                 619     384      35
 
EaeI y'GGCC_r

Cuts at:      0     696    1038 
   Size:        696     342
 
EarI CTCTTCn'nnn_

Cuts at:      0     464     825    1038 
   Size:        464     361     213

  Fragments arranged by size: 

                 464     361     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     167     743     749    1038 
   Size:         54     113     576       6     289

  Fragments arranged by size: 

                 576     289     113      54       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54    1038 
   Size:         54     984
 
EcoRI G'AATT_C

Cuts at:      0     895    1038 
   Size:        895     143
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      33    1038 
   Size:         33    1005
 
HincII GTy'rAC

Cuts at:      0      93    1038 
   Size:         93     945
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     868    1038 
   Size:        868     170
 
MslI CAynn'nnrTG

Cuts at:      0     271     286     698    1038 
   Size:        271      15     412     340

  Fragments arranged by size: 

                 412     340     271      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     721    1038 
   Size:        721     317
 
PsiI TTA'TAA

Cuts at:      0     992    1038 
   Size:        992      46
 
PstI C_TGCA'G

Cuts at:      0     753    1038 
   Size:        753     285
 
SapI GCTCTTCn'nnn_

Cuts at:      0     464    1038 
   Size:        464     574
 
ScaI AGT'ACT

Cuts at:      0     353    1038 
   Size:        353     685
 
SexAI A'CCwGG_T

Cuts at:      0     552    1038 
   Size:        552     486
 
SfcI C'TryA_G

Cuts at:      0     215     749     815    1038 
   Size:        215     534      66     223

  Fragments arranged by size: 

                 534     223     215      66
 
SmlI C'TyrA_G

Cuts at:      0     452    1038 
   Size:        452     586
 
TatI w'GTAC_w

Cuts at:      0     351    1038 
   Size:        351     687
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI     BclI    BglII     BmrI     BpmI    BpuEI
    BseRI     BsmI    BspHI    BsrBI    BssSI    BstXI    BstYI     BtsI
    CspCI     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI   HincII
     MmeI     MslI    PflMI     PsiI     PstI     SapI     ScaI    SexAI
     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI