Restriction map for
pF1KB5549
(Linear) (Six-base) MAPSORT of: KIBB5549.seq Check: 2555 from: 1 to: 4140
KIBB5549.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1080 4140
Size: 1080 3060
AccI GT'mk_AC
Cuts at: 0 2151 3539 4054 4140
Size: 2151 1388 515 86
Fragments arranged by size:
2151 1388 515 86
Acc65I G'GTAC_C
Cuts at: 0 3794 4140
Size: 3794 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1376 1787 2966 3376 4130 4140
Size: 1376 411 1179 410 754 10
Fragments arranged by size:
1376 1179 754 411 410 10
AflIII A'CryG_T
Cuts at: 0 524 4140
Size: 524 3616
AleI CACnn'nnGTG
Cuts at: 0 523 1360 4140
Size: 523 837 2780
Fragments arranged by size:
2780 837 523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3848 4140
Size: 3848 292
AlwNI CAG_nnn'CTG
Cuts at: 0 1183 1213 1417 1765 2533 4108 4140
Size: 1183 30 204 348 768 1575 32
Fragments arranged by size:
1575 1183 768 348 204 32 30
ApaI G_GGCC'C
Cuts at: 0 356 4140
Size: 356 3784
ApoI r'AATT_y
Cuts at: 0 1666 1796 1905 2130 3025 3243 4040 4140
Size: 1666 130 109 225 895 218 797 100
Fragments arranged by size:
1666 895 797 225 218 130 109 100
AseI AT'TA_AT
Cuts at: 0 1220 2456 4140
Size: 1220 1236 1684
Fragments arranged by size:
1684 1236 1220
AvaI C'yCGr_G
Cuts at: 0 86 706 2537 4140
Size: 86 620 1831 1603
Fragments arranged by size:
1831 1603 620 86
BamHI G'GATC_C
Cuts at: 0 731 4140
Size: 731 3409
BanI G'GyrC_C
Cuts at: 0 139 702 3794 4140
Size: 139 563 3092 346
Fragments arranged by size:
3092 563 346 139
BanII G_rGCy'C
Cuts at: 0 82 356 3132 4140
Size: 82 274 2776 1008
Fragments arranged by size:
2776 1008 274 82
BbeI G_GCGC'C
Cuts at: 0 143 4140
Size: 143 3997
BbsI GAAGACnn'nnnn_
Cuts at: 0 944 4140
Size: 944 3196
BbvCI CC'TCA_GC
Cuts at: 0 202 4140
Size: 202 3938
BciVI GTATCCnnnnn_n'
Cuts at: 0 2180 2986 3183 4140
Size: 2180 806 197 957
Fragments arranged by size:
2180 957 806 197
BclI T'GATC_A
Cuts at: 0 1118 4140
Size: 1118 3022
BfrBI ATG'CAT
Cuts at: 0 414 1689 4140
Size: 414 1275 2451
Fragments arranged by size:
2451 1275 414
BglI GCCn_nnn'nGGC
Cuts at: 0 30 33 91 148 230 384 4140
Size: 30 3 58 57 82 154 3756
Fragments arranged by size:
3756 154 82 58 57 30 3
BglII A'GATC_T
Cuts at: 0 3604 4140
Size: 3604 536
Bme1580I G_kGCm'C
Cuts at: 0 356 570 707 4140
Size: 356 214 137 3433
Fragments arranged by size:
3433 356 214 137
BmgBI CAC'GTC
Cuts at: 0 525 4140
Size: 525 3615
BmrI ACTGGGnnnn_n'
Cuts at: 0 1313 2458 3407 4140
Size: 1313 1145 949 733
Fragments arranged by size:
1313 1145 949 733
BmtI G_CTAG'C
Cuts at: 0 1433 2663 4140
Size: 1433 1230 1477
Fragments arranged by size:
1477 1433 1230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 2504 4140
Size: 794 1710 1636
Fragments arranged by size:
1710 1636 794
Bpu10I CC'TnA_GC
Cuts at: 0 202 4009 4078 4140
Size: 202 3807 69 62
Fragments arranged by size:
3807 202 69 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1557 2329 3116 4140
Size: 1557 772 787 1024
Fragments arranged by size:
1557 1024 787 772
BsaI GGTCTCn'nnnn_
Cuts at: 0 418 3217 4140
Size: 418 2799 923
Fragments arranged by size:
2799 923 418
BsaHI Gr'CG_yC
Cuts at: 0 140 2384 2496 4140
Size: 140 2244 112 1644
Fragments arranged by size:
2244 1644 140 112
BsaWI w'CCGG_w
Cuts at: 0 3525 3872 4140
Size: 3525 347 268
Fragments arranged by size:
3525 347 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 4140
Size: 178 30 3932
Fragments arranged by size:
3932 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 2754 2757 3464 4140
Size: 199 2555 3 707 676
Fragments arranged by size:
2555 707 676 199 3
BseYI C'CCAG_C
Cuts at: 0 72 538 3054 4140
Size: 72 466 2516 1086
Fragments arranged by size:
2516 1086 466 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3554 4140
Size: 3554 586
BsiEI CG_ry'CG
Cuts at: 0 12 25 152 168 218 258 277 384
Size: 12 13 127 16 50 40 19 107
Cuts at: 384 473 621 639 4140
Size: 89 148 18 3501
Fragments arranged by size:
3501 148 127 107 89 50 40 19
18 16 13 12
BsiHKAI G_wGCw'C
Cuts at: 0 82 2887 3132 4140
Size: 82 2805 245 1008
Fragments arranged by size:
2805 1008 245 82
BsmI GAATG_Cn'
Cuts at: 0 1200 2240 4140
Size: 1200 1040 1900
Fragments arranged by size:
1900 1200 1040
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2503 4140
Size: 2503 1637
Bsp1286I G_dGCh'C
Cuts at: 0 82 356 570 707 2887 3132 4140
Size: 82 274 214 137 2180 245 1008
Fragments arranged by size:
2180 1008 274 245 214 137 82
BspEI T'CCGG_A
Cuts at: 0 3525 4140
Size: 3525 615
BspHI T'CATG_A
Cuts at: 0 3828 4020 4140
Size: 3828 192 120
Fragments arranged by size:
3828 192 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 1080 2015 4140
Size: 539 541 935 2125
Fragments arranged by size:
2125 935 541 539
BsrBI CCG'CTC
Cuts at: 0 8 21 260 4140
Size: 8 13 239 3880
Fragments arranged by size:
3880 239 13 8
BsrFI r'CCGG_y
Cuts at: 0 272 301 383 472 676 4057 4140
Size: 272 29 82 89 204 3381 83
Fragments arranged by size:
3381 272 204 89 83 82 29
BssHII G'CGCG_C
Cuts at: 0 714 4140
Size: 714 3426
BssSI C'ACGA_G
Cuts at: 0 3173 4140
Size: 3173 967
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1209 1362 2330 3402 4140
Size: 1209 153 968 1072 738
Fragments arranged by size:
1209 1072 968 738 153
BstYI r'GATC_y
Cuts at: 0 731 1180 1935 3604 4140
Size: 731 449 755 1669 536
Fragments arranged by size:
1669 755 731 536 449
BstZ17I GTA'TAC
Cuts at: 0 3540 4140
Size: 3540 600
Bsu36I CC'TnA_GG
Cuts at: 0 323 4140
Size: 323 3817
BtgI C'CryG_G
Cuts at: 0 218 483 2979 4140
Size: 218 265 2496 1161
Fragments arranged by size:
2496 1161 265 218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3768 4140
Size: 3768 372
ClaI AT'CG_AT
Cuts at: 0 2793 4140
Size: 2793 1347
DraI TTT'AAA
Cuts at: 0 864 4140
Size: 864 3276
DraIII CAC_nnn'GTG
Cuts at: 0 2466 4140
Size: 2466 1674
DrdI GACnn_nn'nnGTC
Cuts at: 0 1768 4140
Size: 1768 2372
EaeI y'GGCC_r
Cuts at: 0 9 22 149 215 255 274 381 470
Size: 9 13 127 66 40 19 107 89
Cuts at: 470 618 636 3516 4140
Size: 148 18 2880 624
Fragments arranged by size:
2880 624 148 127 107 89 66 40
19 18 13 9
EagI C'GGCC_G
Cuts at: 0 9 22 149 215 255 274 381 470
Size: 9 13 127 66 40 19 107 89
Cuts at: 470 618 636 4140
Size: 148 18 3504
Fragments arranged by size:
3504 148 127 107 89 66 40 19
18 13 9
EarI CTCTTCn'nnn_
Cuts at: 0 176 1704 3614 4106 4140
Size: 176 1528 1910 492 34
Fragments arranged by size:
1910 1528 492 176 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 493 4140
Size: 124 369 3647
Fragments arranged by size:
3647 369 124
EcoICRI GAG'CTC
Cuts at: 0 80 3130 4140
Size: 80 3050 1010
Fragments arranged by size:
3050 1010 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1376 1787 2504 2966 3376 4130 4140
Size: 794 582 411 717 462 410 754 10
Fragments arranged by size:
794 754 717 582 462 411 410 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 4140
Size: 697 3443
EcoO109I rG'GnC_Cy
Cuts at: 0 337 489 4140
Size: 337 152 3651
Fragments arranged by size:
3651 337 152
EcoRI G'AATT_C
Cuts at: 0 1796 4140
Size: 1796 2344
EcoRV GAT'ATC
Cuts at: 0 1628 4140
Size: 1628 2512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 812 4014 4140
Size: 812 3202 126
Fragments arranged by size:
3202 812 126
FseI GG_CCGG'CC
Cuts at: 0 276 387 4140
Size: 276 111 3753
Fragments arranged by size:
3753 276 111
HaeII r_GCGC'y
Cuts at: 0 143 202 2588 3760 4140
Size: 143 59 2386 1172 380
Fragments arranged by size:
2386 1172 380 143 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 415 447 1405 1437 1920 1952
Size: 178 32 205 32 958 32 483 32
Cuts at: 1952 2339 2371 4140
Size: 387 32 1769
Fragments arranged by size:
1769 958 483 387 205 178 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2031 2598 3075 4140
Size: 2031 567 477 1065
Fragments arranged by size:
2031 1065 567 477
HindIII A'AGCT_T
Cuts at: 0 654 878 4140
Size: 654 224 3262
Fragments arranged by size:
3262 654 224
HpaI GTT'AAC
Cuts at: 0 2031 4140
Size: 2031 2109
KasI G'GCGC_C
Cuts at: 0 139 4140
Size: 139 4001
KpnI G_GTAC'C
Cuts at: 0 3798 4140
Size: 3798 342
MfeI C'AATT_G
Cuts at: 0 3067 4140
Size: 3067 1073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1686 2124 2713 2976 4140
Size: 170 1516 438 589 263 1164
Fragments arranged by size:
1516 1164 589 438 263 170
MscI TGG'CCA
Cuts at: 0 3518 4140
Size: 3518 622
MslI CAynn'nnrTG
Cuts at: 0 523 980 1147 1207 1360 1694 2984 3400
Size: 523 457 167 60 153 334 1290 416
Cuts at: 3400 3830 3992 4022 4140
Size: 430 162 30 118
Fragments arranged by size:
1290 523 457 430 416 334 167 162
153 118 60 30
MspA1I CmG'CkG
Cuts at: 0 220 279 372 485 617 2731 3058 4140
Size: 220 59 93 113 132 2114 327 1082
Fragments arranged by size:
2114 1082 327 220 132 113 93 59
NaeI GCC'GGC
Cuts at: 0 274 303 385 474 4140
Size: 274 29 82 89 3666
Fragments arranged by size:
3666 274 89 82 29
NarI GG'CG_CC
Cuts at: 0 140 4140
Size: 140 4000
NcoI C'CATG_G
Cuts at: 0 2979 4140
Size: 2979 1161
NgoMIV G'CCGG_C
Cuts at: 0 272 301 383 472 4140
Size: 272 29 82 89 3668
Fragments arranged by size:
3668 272 89 82 29
NheI G'CTAG_C
Cuts at: 0 1429 2659 4140
Size: 1429 1230 1481
Fragments arranged by size:
1481 1429 1230
NotI GC'GGCC_GC
Cuts at: 0 22 215 618 636 4140
Size: 22 193 403 18 3504
Fragments arranged by size:
3504 403 193 22 18
NsiI A_TGCA'T
Cuts at: 0 416 1691 4140
Size: 416 1275 2449
Fragments arranged by size:
2449 1275 416
NspI r_CATG'y
Cuts at: 0 414 4140
Size: 414 3726
PacI TTA_AT'TAA
Cuts at: 0 2717 4140
Size: 2717 1423
PfoI T'CCnGG_A
Cuts at: 0 927 1272 2508 3444 4091 4140
Size: 927 345 1236 936 647 49
Fragments arranged by size:
1236 936 927 647 345 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 2381 2413 4140
Size: 145 32 2204 32 1727
Fragments arranged by size:
2204 1727 145 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 489 4140
Size: 489 3651
PshAI GACnn'nnGTC
Cuts at: 0 629 3266 4140
Size: 629 2637 874
Fragments arranged by size:
2637 874 629
PsiI TTA'TAA
Cuts at: 0 2519 4140
Size: 2519 1621
PspOMI G'GGCC_C
Cuts at: 0 352 4140
Size: 352 3788
PspXI vC'TCGA_Gb
Cuts at: 0 2537 4140
Size: 2537 1603
PvuII CAG'CTG
Cuts at: 0 3058 4140
Size: 3058 1082
SacI G_AGCT'C
Cuts at: 0 82 3132 4140
Size: 82 3050 1008
Fragments arranged by size:
3050 1008 82
SacII CC_GC'GG
Cuts at: 0 221 486 4140
Size: 221 265 3654
Fragments arranged by size:
3654 265 221
SanDI GG'GwC_CC
Cuts at: 0 489 4140
Size: 489 3651
SexAI A'CCwGG_T
Cuts at: 0 1653 4140
Size: 1653 2487
SfcI C'TryA_G
Cuts at: 0 834 4140
Size: 834 3306
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 230 4140
Size: 230 3910
SfoI GGC'GCC
Cuts at: 0 141 4140
Size: 141 3999
SgrAI Cr'CCGG_yG
Cuts at: 0 676 4140
Size: 676 3464
SmaI CCC'GGG
Cuts at: 0 88 4140
Size: 88 4052
SmlI C'TyrA_G
Cuts at: 0 1536 2308 2537 3131 4140
Size: 1536 772 229 594 1009
Fragments arranged by size:
1536 1009 772 594 229
SrfI GCCC'GGGC
Cuts at: 0 88 4140
Size: 88 4052
SspI AAT'ATT
Cuts at: 0 1452 2182 3441 4140
Size: 1452 730 1259 699
Fragments arranged by size:
1452 1259 730 699
StyI C'CwwG_G
Cuts at: 0 28 1583 2123 2979 3889 4140
Size: 28 1555 540 856 910 251
Fragments arranged by size:
1555 910 856 540 251 28
SwaI ATTT'AAAT
Cuts at: 0 864 4140
Size: 864 3276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 2477 4140
Size: 273 2204 1663
Fragments arranged by size:
2204 1663 273
TatI w'GTAC_w
Cuts at: 0 1673 1992 3145 3327 3696 3747 4140
Size: 1673 319 1153 182 369 51 393
Fragments arranged by size:
1673 1153 393 369 319 182 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1685 1717 2890 2922 4140
Size: 1685 32 1173 32 1218
Fragments arranged by size:
1685 1218 1173 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 429 4140
Size: 429 3711
XbaI T'CTAG_A
Cuts at: 0 2400 4140
Size: 2400 1740
XhoI C'TCGA_G
Cuts at: 0 2537 4140
Size: 2537 1603
XmaI C'CCGG_G
Cuts at: 0 86 4140
Size: 86 4054
XmnI GAAnn'nnTTC
Cuts at: 0 1136 2183 4140
Size: 1136 1047 1957
Fragments arranged by size:
1957 1136 1047
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NsiI NspI PacI PfoI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuII SacI SacII SanDI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SrfI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApaLI
AscI AsiSI AvrII BaeI BcgI BlpI BplI BsaAI
BsaBI BsiWI BsrDI BsrGI BstAPI BstBI BstEII BtsI
CspCI FspI FspAI MluI NdeI NruI PasI PciI
PflMI PmeI PmlI PsrI PstI PvuI RsrII SalI
SapI SbfI ScaI SgfI SnaBI SpeI SphI StuI
XcmI ZraI