Restriction map for
pF1KB5554
(Linear) (Six-base) MAPSORT of: KIBB5554.seq Check: 467 from: 1 to: 894
KIBB5554.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 35 894
Size: 35 859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 460 894
Size: 217 243 434
Fragments arranged by size:
434 243 217
AflIII A'CryG_T
Cuts at: 0 621 894
Size: 621 273
AhdI GACnn_n'nnGTC
Cuts at: 0 733 771 894
Size: 733 38 123
Fragments arranged by size:
733 123 38
ApaLI G'TGCA_C
Cuts at: 0 224 894
Size: 224 670
ApoI r'AATT_y
Cuts at: 0 305 894
Size: 305 589
BanI G'GyrC_C
Cuts at: 0 846 894
Size: 846 48
BanII G_rGCy'C
Cuts at: 0 158 511 894
Size: 158 353 383
Fragments arranged by size:
383 353 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 685 894
Size: 29 656 209
Fragments arranged by size:
656 209 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 350 582 616 894
Size: 316 34 232 34 278
Fragments arranged by size:
316 278 232 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 158 894
Size: 158 736
BfrBI ATG'CAT
Cuts at: 0 603 894
Size: 603 291
Bme1580I G_kGCm'C
Cuts at: 0 228 894
Size: 228 666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 894
Size: 419 475
BsaAI yAC'GTr
Cuts at: 0 199 894
Size: 199 695
BsaBI GATnn'nnATC
Cuts at: 0 128 894
Size: 128 766
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 894
Size: 518 376
BsiHKAI G_wGCw'C
Cuts at: 0 158 228 511 533 894
Size: 158 70 283 22 361
Fragments arranged by size:
361 283 158 70 22
Bsp1286I G_dGCh'C
Cuts at: 0 158 228 511 533 894
Size: 158 70 283 22 361
Fragments arranged by size:
361 283 158 70 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 76 528 894
Size: 76 452 366
Fragments arranged by size:
452 366 76
Bsu36I CC'TnA_GG
Cuts at: 0 887 894
Size: 887 7
BtgI C'CryG_G
Cuts at: 0 212 894
Size: 212 682
DraI TTT'AAA
Cuts at: 0 182 639 894
Size: 182 457 255
Fragments arranged by size:
457 255 182
DraIII CAC_nnn'GTG
Cuts at: 0 528 894
Size: 528 366
EaeI y'GGCC_r
Cuts at: 0 209 894
Size: 209 685
EcoICRI GAG'CTC
Cuts at: 0 156 509 894
Size: 156 353 385
Fragments arranged by size:
385 353 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 419 460 894
Size: 217 202 41 434
Fragments arranged by size:
434 217 202 41
EcoO109I rG'GnC_Cy
Cuts at: 0 578 894
Size: 578 316
EcoRI G'AATT_C
Cuts at: 0 305 894
Size: 305 589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 719 751 757 789 894
Size: 719 32 6 32 105
Fragments arranged by size:
719 105 32 32 6
HincII GTy'rAC
Cuts at: 0 776 894
Size: 776 118
HindIII A'AGCT_T
Cuts at: 0 101 894
Size: 101 793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 894
Size: 282 612
NsiI A_TGCA'T
Cuts at: 0 605 894
Size: 605 289
NspI r_CATG'y
Cuts at: 0 625 894
Size: 625 269
PciI A'CATG_T
Cuts at: 0 621 894
Size: 621 273
PstI C_TGCA'G
Cuts at: 0 681 894
Size: 681 213
SacI G_AGCT'C
Cuts at: 0 158 511 894
Size: 158 353 383
Fragments arranged by size:
383 353 158
ScaI AGT'ACT
Cuts at: 0 473 894
Size: 473 421
SfcI C'TryA_G
Cuts at: 0 677 788 894
Size: 677 111 106
Fragments arranged by size:
677 111 106
TatI w'GTAC_w
Cuts at: 0 471 894
Size: 471 423
Enzymes that do cut:
AccI AcuI AflIII AhdI ApaLI ApoI BanI BanII
BbsI BcgI BciVI BfrBI Bme1580I BpmI BsaAI BsaBI
BsgI BsiHKAI Bsp1286I BstAPI Bsu36I BtgI DraI DraIII
EaeI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII
MmeI NsiI NspI PciI PstI SacI ScaI SfcI
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BclI BglI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DrdI EagI EarI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI