Restriction map for
pF1KB5556
(Linear) (Six-base) MAPSORT of: KIBB5556.seq Check: 4906 from: 1 to: 993
KIBB5556.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 638 993
Size: 638 355
AccI GT'mk_AC
Cuts at: 0 506 993
Size: 506 487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 459 694 993
Size: 166 293 235 299
Fragments arranged by size:
299 293 235 166
AhdI GACnn_n'nnGTC
Cuts at: 0 741 993
Size: 741 252
AlwNI CAG_nnn'CTG
Cuts at: 0 291 672 993
Size: 291 381 321
Fragments arranged by size:
381 321 291
AvaI C'yCGr_G
Cuts at: 0 220 697 993
Size: 220 477 296
Fragments arranged by size:
477 296 220
BanI G'GyrC_C
Cuts at: 0 558 956 993
Size: 558 398 37
Fragments arranged by size:
558 398 37
BbeI G_GCGC'C
Cuts at: 0 562 993
Size: 562 431
BbvCI CC'TCA_GC
Cuts at: 0 517 993
Size: 517 476
BglI GCCn_nnn'nGGC
Cuts at: 0 448 583 993
Size: 448 135 410
Fragments arranged by size:
448 410 135
BlpI GC'TnA_GC
Cuts at: 0 541 993
Size: 541 452
Bme1580I G_kGCm'C
Cuts at: 0 260 298 698 993
Size: 260 38 400 295
Fragments arranged by size:
400 295 260 38
BmgBI CAC'GTC
Cuts at: 0 195 392 993
Size: 195 197 601
Fragments arranged by size:
601 197 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 279 993
Size: 127 152 714
Fragments arranged by size:
714 152 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 841 859 993
Size: 247 594 18 134
Fragments arranged by size:
594 247 134 18
Bpu10I CC'TnA_GC
Cuts at: 0 517 901 993
Size: 517 384 92
Fragments arranged by size:
517 384 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 993
Size: 190 803
BsaHI Gr'CG_yC
Cuts at: 0 214 559 883 993
Size: 214 345 324 110
Fragments arranged by size:
345 324 214 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 868 898 993
Size: 868 30 95
Fragments arranged by size:
868 95 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 694 993
Size: 169 525 299
Fragments arranged by size:
525 299 169
BseYI C'CCAG_C
Cuts at: 0 382 602 635 993
Size: 382 220 33 358
Fragments arranged by size:
382 358 220 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 472 670 993
Size: 346 126 198 323
Fragments arranged by size:
346 323 198 126
BsiEI CG_ry'CG
Cuts at: 0 578 993
Size: 578 415
Bsp1286I G_dGCh'C
Cuts at: 0 260 298 698 993
Size: 260 38 400 295
Fragments arranged by size:
400 295 260 38
BspHI T'CATG_A
Cuts at: 0 935 993
Size: 935 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 638 993
Size: 638 355
BsrBI CCG'CTC
Cuts at: 0 68 753 993
Size: 68 685 240
Fragments arranged by size:
685 240 68
BsrFI r'CCGG_y
Cuts at: 0 405 501 577 993
Size: 405 96 76 416
Fragments arranged by size:
416 405 96 76
BssHII G'CGCG_C
Cuts at: 0 345 536 993
Size: 345 191 457
Fragments arranged by size:
457 345 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 630 981 993
Size: 630 351 12
Fragments arranged by size:
630 351 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 993
Size: 738 255
BstYI r'GATC_y
Cuts at: 0 95 993
Size: 95 898
BtgI C'CryG_G
Cuts at: 0 434 658 887 993
Size: 434 224 229 106
Fragments arranged by size:
434 229 224 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 774 993
Size: 774 219
EaeI y'GGCC_r
Cuts at: 0 125 353 575 890 993
Size: 125 228 222 315 103
Fragments arranged by size:
315 228 222 125 103
EagI C'GGCC_G
Cuts at: 0 575 993
Size: 575 418
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 778 993
Size: 778 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 247 459 694 841 859 993
Size: 166 81 212 235 147 18 134
Fragments arranged by size:
235 212 166 147 134 81 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 676 993
Size: 676 317
EcoO109I rG'GnC_Cy
Cuts at: 0 669 993
Size: 669 324
FseI GG_CCGG'CC
Cuts at: 0 581 993
Size: 581 412
HaeII r_GCGC'y
Cuts at: 0 234 482 562 993
Size: 234 248 80 431
Fragments arranged by size:
431 248 234 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 868 900 993
Size: 727 32 109 32 93
Fragments arranged by size:
727 109 93 32 32
HincII GTy'rAC
Cuts at: 0 411 948 993
Size: 411 537 45
Fragments arranged by size:
537 411 45
HpaI GTT'AAC
Cuts at: 0 948 993
Size: 948 45
KasI G'GCGC_C
Cuts at: 0 558 993
Size: 558 435
MslI CAynn'nnrTG
Cuts at: 0 736 993
Size: 736 257
MspA1I CmG'CkG
Cuts at: 0 436 461 467 548 660 993
Size: 436 25 6 81 112 333
Fragments arranged by size:
436 333 112 81 25 6
NaeI GCC'GGC
Cuts at: 0 579 993
Size: 579 414
NarI GG'CG_CC
Cuts at: 0 559 993
Size: 559 434
NgoMIV G'CCGG_C
Cuts at: 0 577 993
Size: 577 416
NspI r_CATG'y
Cuts at: 0 900 993
Size: 900 93
PstI C_TGCA'G
Cuts at: 0 668 993
Size: 668 325
SacII CC_GC'GG
Cuts at: 0 437 661 993
Size: 437 224 332
Fragments arranged by size:
437 332 224
SfcI C'TryA_G
Cuts at: 0 81 664 993
Size: 81 583 329
Fragments arranged by size:
583 329 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 583 993
Size: 583 410
SfoI GGC'GCC
Cuts at: 0 560 993
Size: 560 433
SmlI C'TyrA_G
Cuts at: 0 205 993
Size: 205 788
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 944 993
Size: 298 646 49
Fragments arranged by size:
646 298 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 949 993
Size: 949 44
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI AvaI BanI BbeI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI
BtgI BtgZI EaeI EagI EciI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HincII HpaI KasI MslI MspA1I
NaeI NarI NgoMIV NspI PstI SacII SfcI SfiI
SfoI SmlI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BcgI BciVI BclI
BfrBI BglII BmtI BplI BsaI BsaAI BsaBI BsaWI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FspI
FspAI HindIII KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI