Restriction map for
pF1KB5558
(Linear) (Six-base) MAPSORT of: KIBB5558.seq Check: 8799 from: 1 to: 1719
KIBB5558.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1438 1719
Size: 1438 281
AccI GT'mk_AC
Cuts at: 0 131 1719
Size: 131 1588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 845 868 1231 1719
Size: 515 330 23 363 488
Fragments arranged by size:
515 488 363 330 23
AgeI A'CCGG_T
Cuts at: 0 36 1719
Size: 36 1683
ApoI r'AATT_y
Cuts at: 0 91 712 749 1418 1643 1719
Size: 91 621 37 669 225 76
Fragments arranged by size:
669 621 225 91 76 37
AseI AT'TA_AT
Cuts at: 0 686 1719
Size: 686 1033
AvaI C'yCGr_G
Cuts at: 0 68 107 1719
Size: 68 39 1612
Fragments arranged by size:
1612 68 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 874 1010 1719
Size: 874 136 709
Fragments arranged by size:
874 709 136
BciVI GTATCCnnnnn_n'
Cuts at: 0 769 1719
Size: 769 950
BfrBI ATG'CAT
Cuts at: 0 1340 1719
Size: 1340 379
Bme1580I G_kGCm'C
Cuts at: 0 1329 1719
Size: 1329 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1349 1541 1719
Size: 890 459 192 178
Fragments arranged by size:
890 459 192 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 27 1719
Size: 27 1692
BsaWI w'CCGG_w
Cuts at: 0 36 1719
Size: 36 1683
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1190 1220 1514 1544 1719
Size: 1190 30 294 30 175
Fragments arranged by size:
1190 294 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 47 1719
Size: 47 1672
BseYI C'CCAG_C
Cuts at: 0 325 1719
Size: 325 1394
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 986 1719
Size: 119 867 733
Fragments arranged by size:
867 733 119
BsmI GAATG_Cn'
Cuts at: 0 1340 1449 1719
Size: 1340 109 270
Fragments arranged by size:
1340 270 109
Bsp1286I G_dGCh'C
Cuts at: 0 1329 1719
Size: 1329 390
BspHI T'CATG_A
Cuts at: 0 1017 1719
Size: 1017 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 1438 1719
Size: 783 655 281
Fragments arranged by size:
783 655 281
BsrDI GCAATG_nn'
Cuts at: 0 572 1433 1685 1719
Size: 572 861 252 34
Fragments arranged by size:
861 572 252 34
BsrFI r'CCGG_y
Cuts at: 0 36 1719
Size: 36 1683
BstAPI GCAn_nnn'nTGC
Cuts at: 0 511 1719
Size: 511 1208
BstEII G'GTnAC_C
Cuts at: 0 8 1719
Size: 8 1711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 1719
Size: 51 1668
BstYI r'GATC_y
Cuts at: 0 1459 1719
Size: 1459 260
BtsI GCAGTG_nn'
Cuts at: 0 341 974 1719
Size: 341 633 745
Fragments arranged by size:
745 633 341
EaeI y'GGCC_r
Cuts at: 0 152 1719
Size: 152 1567
EarI CTCTTCn'nnn_
Cuts at: 0 974 1649 1719
Size: 974 675 70
Fragments arranged by size:
974 675 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 845 868 890 1231 1349 1541 1719
Size: 515 330 23 22 341 118 192 178
Fragments arranged by size:
515 341 330 192 178 118 23 22
EcoO109I rG'GnC_Cy
Cuts at: 0 1662 1719
Size: 1662 57
EcoRI G'AATT_C
Cuts at: 0 712 1719
Size: 712 1007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1719
Size: 396 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1013 1719
Size: 981 32 706
Fragments arranged by size:
981 706 32
HincII GTy'rAC
Cuts at: 0 1616 1719
Size: 1616 103
HpaI GTT'AAC
Cuts at: 0 1616 1719
Size: 1616 103
MfeI C'AATT_G
Cuts at: 0 248 1719
Size: 248 1471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 1014 1719
Size: 558 456 705
Fragments arranged by size:
705 558 456
NsiI A_TGCA'T
Cuts at: 0 1342 1719
Size: 1342 377
NspI r_CATG'y
Cuts at: 0 1716 1719
Size: 1716 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 913 1719
Size: 913 806
PfoI T'CCnGG_A
Cuts at: 0 716 1719
Size: 716 1003
PpuMI rG'GwC_Cy
Cuts at: 0 1662 1719
Size: 1662 57
PshAI GACnn'nnGTC
Cuts at: 0 39 1719
Size: 39 1680
SfcI C'TryA_G
Cuts at: 0 365 1619 1719
Size: 365 1254 100
Fragments arranged by size:
1254 365 100
SpeI A'CTAG_T
Cuts at: 0 1144 1719
Size: 1144 575
StyI C'CwwG_G
Cuts at: 0 371 1349 1719
Size: 371 978 370
Fragments arranged by size:
978 371 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1663 1672 1719
Size: 1663 9 47
Fragments arranged by size:
1663 47 9
TatI w'GTAC_w
Cuts at: 0 351 850 1719
Size: 351 499 869
Fragments arranged by size:
869 499 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 167 199 1190 1222 1514 1546 1629 1661
Size: 167 32 991 32 292 32 83 32
Cuts at: 1661 1719
Size: 58
Fragments arranged by size:
991 292 167 83 58 32 32 32
32
XmnI GAAnn'nnTTC
Cuts at: 0 1007 1719
Size: 1007 712
Enzymes that do cut:
AarI AccI AcuI AgeI ApoI AseI AvaI BbsI
BciVI BfrBI Bme1580I BpmI BsaI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstAPI BstEII BstXI BstYI BtsI EaeI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI MmeI
NsiI NspI PflMI PfoI PpuMI PshAI SfcI SpeI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII KasI KpnI MluI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI