(Linear) (Six-base) MAPSORT of: KIBB5561.seq  Check: 6067  from: 1  to: 1491



KIBB5561.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:      0    1347    1452    1491 
   Size:       1347     105      39

  Fragments arranged by size: 

                1347     105      39
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1031    1097    1491 
   Size:        427     604      66     394

  Fragments arranged by size: 

                 604     427     394      66
 
AflIII A'CryG_T

Cuts at:      0      32    1405    1491 
   Size:         32    1373      86

  Fragments arranged by size: 

                1373      86      32
 
ApaI G_GGCC'C

Cuts at:      0     990    1491 
   Size:        990     501
 
ApaLI G'TGCA_C

Cuts at:      0     143    1491 
   Size:        143    1348
 
ApoI r'AATT_y

Cuts at:      0     528     760     905    1491 
   Size:        528     232     145     586

  Fragments arranged by size: 

                 586     528     232     145
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     365     398    1491 
   Size:        365      33    1093

  Fragments arranged by size: 

                1093     365      33
 
BanII G_rGCy'C

Cuts at:      0     990    1491 
   Size:        990     501
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1199    1491 
   Size:       1199     292
 
BclI T'GATC_A

Cuts at:      0     417    1491 
   Size:        417    1074
 
Bme1580I G_kGCm'C

Cuts at:      0     147     990    1491 
   Size:        147     843     501

  Fragments arranged by size: 

                 843     501     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1438    1491 
   Size:       1438      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     912    1491 
   Size:        912     579
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      85    1491 
   Size:         85    1406
 
BsaHI Gr'CG_yC

Cuts at:      0     670    1491 
   Size:        670     821
 
BsaWI w'CCGG_w

Cuts at:      0     681    1491 
   Size:        681     810
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21    1491 
   Size:         21    1470
 
BsiHKAI G_wGCw'C

Cuts at:      0     147     305    1491 
   Size:        147     158    1186

  Fragments arranged by size: 

                1186     158     147
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     305     990    1491 
   Size:        147     158     685     501

  Fragments arranged by size: 

                 685     501     158     147
 
BspEI T'CCGG_A

Cuts at:      0     681    1491 
   Size:        681     810
 
BsrDI GCAATG_nn'

Cuts at:      0     112     948     969    1491 
   Size:        112     836      21     522

  Fragments arranged by size: 

                 836     522     112      21
 
BsrGI T'GTAC_A

Cuts at:      0     432    1491 
   Size:        432    1059
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     938    1491 
   Size:        938     553
 
BstYI r'GATC_y

Cuts at:      0     684     897    1491 
   Size:        684     213     594

  Fragments arranged by size: 

                 684     594     213
 
BtgI C'CryG_G

Cuts at:      0    1371    1491 
   Size:       1371     120
 
BtsI GCAGTG_nn'

Cuts at:      0     660    1491 
   Size:        660     831
 
EaeI y'GGCC_r

Cuts at:      0     189    1491 
   Size:        189    1302
 
EarI CTCTTCn'nnn_

Cuts at:      0     443    1073    1280    1491 
   Size:        443     630     207     211

  Fragments arranged by size: 

                 630     443     211     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1031    1097    1491 
   Size:        427     604      66     394

  Fragments arranged by size: 

                 604     427     394      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     426     987    1491 
   Size:        426     561     504

  Fragments arranged by size: 

                 561     504     426
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     193    1491 
   Size:        193    1298
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     526     558     803     835    1491 
   Size:        526      32     245      32     656

  Fragments arranged by size: 

                 656     526     245      32      32
 
HincII GTy'rAC

Cuts at:      0    1348    1491 
   Size:       1348     143
 
HindIII A'AGCT_T

Cuts at:      0     285    1169    1491 
   Size:        285     884     322

  Fragments arranged by size: 

                 884     322     285
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     704    1491 
   Size:        704     787
 
MscI TGG'CCA

Cuts at:      0     191    1491 
   Size:        191    1300
 
MslI CAynn'nnrTG

Cuts at:      0     713    1491 
   Size:        713     778
 
NcoI C'CATG_G

Cuts at:      0    1371    1491 
   Size:       1371     120
 
NspI r_CATG'y

Cuts at:      0      36    1409    1491 
   Size:         36    1373      82

  Fragments arranged by size: 

                1373      82      36
 
PciI A'CATG_T

Cuts at:      0      32    1405    1491 
   Size:         32    1373      86

  Fragments arranged by size: 

                1373      86      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     426    1491 
   Size:        426    1065
 
PspOMI G'GGCC_C

Cuts at:      0     986    1491 
   Size:        986     505
 
SalI G'TCGA_C

Cuts at:      0    1346    1491 
   Size:       1346     145
 
SfcI C'TryA_G

Cuts at:      0     327     662    1112    1491 
   Size:        327     335     450     379

  Fragments arranged by size: 

                 450     379     335     327
 
StyI C'CwwG_G

Cuts at:      0    1371    1491 
   Size:       1371     120
 
TatI w'GTAC_w

Cuts at:      0     432    1491 
   Size:        432    1059
 
XbaI T'CTAG_A

Cuts at:      0     478     496    1491 
   Size:        478      18     995

  Fragments arranged by size: 

                 995     478      18
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApaI    ApaLI     ApoI     BaeI    BanII
     BbsI     BclI Bme1580I     BmrI   Bpu10I     BsaI    BsaHI    BsaWI
    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI    BsrGI   BstAPI    BstYI
     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI     NcoI     NspI     PciI
    PpuMI   PspOMI     SalI     SfcI     StyI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI