Restriction map for
pF1KB5566
(Linear) (Six-base) MAPSORT of: KIBB5566.seq Check: 4721 from: 1 to: 792
KIBB5566.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 745 792
Size: 745 47
AgeI A'CCGG_T
Cuts at: 0 689 792
Size: 689 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 673 792
Size: 673 119
AlwNI CAG_nnn'CTG
Cuts at: 0 579 792
Size: 579 213
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 261 294 792
Size: 261 33 498
Fragments arranged by size:
498 261 33
BamHI G'GATC_C
Cuts at: 0 96 217 792
Size: 96 121 575
Fragments arranged by size:
575 121 96
BanI G'GyrC_C
Cuts at: 0 289 792
Size: 289 503
BclI T'GATC_A
Cuts at: 0 776 792
Size: 776 16
BlpI GC'TnA_GC
Cuts at: 0 572 613 792
Size: 572 41 179
Fragments arranged by size:
572 179 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 768 792
Size: 768 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 792
Size: 616 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 745 792
Size: 745 47
BsaHI Gr'CG_yC
Cuts at: 0 103 792
Size: 103 689
BsaWI w'CCGG_w
Cuts at: 0 25 689 792
Size: 25 664 103
Fragments arranged by size:
664 103 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 792
Size: 370 422
BseYI C'CCAG_C
Cuts at: 0 213 285 792
Size: 213 72 507
Fragments arranged by size:
507 213 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 792
Size: 92 700
BsmI GAATG_Cn'
Cuts at: 0 68 792
Size: 68 724
BspEI T'CCGG_A
Cuts at: 0 25 792
Size: 25 767
BsrFI r'CCGG_y
Cuts at: 0 428 689 792
Size: 428 261 103
Fragments arranged by size:
428 261 103
BsrGI T'GTAC_A
Cuts at: 0 422 792
Size: 422 370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 792
Size: 609 183
BstEII G'GTnAC_C
Cuts at: 0 150 792
Size: 150 642
BstYI r'GATC_y
Cuts at: 0 96 217 792
Size: 96 121 575
Fragments arranged by size:
575 121 96
BtgI C'CryG_G
Cuts at: 0 146 461 792
Size: 146 315 331
Fragments arranged by size:
331 315 146
EaeI y'GGCC_r
Cuts at: 0 336 545 792
Size: 336 209 247
Fragments arranged by size:
336 247 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 792
Size: 203 589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 792
Size: 616 176
EcoO109I rG'GnC_Cy
Cuts at: 0 158 792
Size: 158 634
HaeII r_GCGC'y
Cuts at: 0 435 792
Size: 435 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 110 120 142 792
Size: 88 22 10 22 650
Fragments arranged by size:
650 88 22 22 10
HincII GTy'rAC
Cuts at: 0 270 792
Size: 270 522
MscI TGG'CCA
Cuts at: 0 547 792
Size: 547 245
MslI CAynn'nnrTG
Cuts at: 0 655 792
Size: 655 137
NaeI GCC'GGC
Cuts at: 0 430 792
Size: 430 362
NcoI C'CATG_G
Cuts at: 0 146 461 792
Size: 146 315 331
Fragments arranged by size:
331 315 146
NgoMIV G'CCGG_C
Cuts at: 0 428 792
Size: 428 364
NspI r_CATG'y
Cuts at: 0 213 516 654 792
Size: 213 303 138 138
Fragments arranged by size:
303 213 138 138
PpuMI rG'GwC_Cy
Cuts at: 0 158 792
Size: 158 634
PshAI GACnn'nnGTC
Cuts at: 0 163 741 792
Size: 163 578 51
Fragments arranged by size:
578 163 51
RsrII CG'GwC_CG
Cuts at: 0 23 792
Size: 23 769
SfcI C'TryA_G
Cuts at: 0 381 746 792
Size: 381 365 46
Fragments arranged by size:
381 365 46
SphI G_CATG'C
Cuts at: 0 654 792
Size: 654 138
StyI C'CwwG_G
Cuts at: 0 146 230 461 792
Size: 146 84 231 331
Fragments arranged by size:
331 231 146 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 637 792
Size: 277 360 155
Fragments arranged by size:
360 277 155
TatI w'GTAC_w
Cuts at: 0 422 662 792
Size: 422 240 130
Fragments arranged by size:
422 240 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 778 792
Size: 778 14
Enzymes that do cut:
AccI AgeI AlfI AlwNI BaeI BamHI BanI BclI
BlpI BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsmI BspEI BsrFI BsrGI BstAPI BstEII BstYI
BtgI EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII
MscI MslI NaeI NcoI NgoMIV NspI PpuMI PshAI
RsrII SfcI SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI