Restriction map for
pF1KB5571
(Linear) (Six-base) MAPSORT of: KIBB5571.seq Check: 440 from: 1 to: 2754
KIBB5571.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 2344 2754
Size: 2344 410
Acc65I G'GTAC_C
Cuts at: 0 1423 2754
Size: 1423 1331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 2183 2754
Size: 842 1341 571
Fragments arranged by size:
1341 842 571
AleI CACnn'nnGTG
Cuts at: 0 2174 2754
Size: 2174 580
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1556 1588 2754
Size: 1556 32 1166
Fragments arranged by size:
1556 1166 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2641 2754
Size: 2641 113
ApoI r'AATT_y
Cuts at: 0 390 1813 2754
Size: 390 1423 941
Fragments arranged by size:
1423 941 390
AseI AT'TA_AT
Cuts at: 0 2156 2754
Size: 2156 598
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 915 948 2754
Size: 915 33 1806
Fragments arranged by size:
1806 915 33
BanI G'GyrC_C
Cuts at: 0 1423 2754
Size: 1423 1331
BbsI GAAGACnn'nnnn_
Cuts at: 0 830 1060 2183 2319 2754
Size: 830 230 1123 136 435
Fragments arranged by size:
1123 830 435 230 136
BciVI GTATCCnnnnn_n'
Cuts at: 0 916 2754
Size: 916 1838
BclI T'GATC_A
Cuts at: 0 689 2754
Size: 689 2065
BfrBI ATG'CAT
Cuts at: 0 2198 2754
Size: 2198 556
BglII A'GATC_T
Cuts at: 0 2118 2382 2754
Size: 2118 264 372
Fragments arranged by size:
2118 372 264
BlpI GC'TnA_GC
Cuts at: 0 1878 2754
Size: 1878 876
BmrI ACTGGGnnnn_n'
Cuts at: 0 1986 2754
Size: 1986 768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 359 948 957 1475 2754
Size: 89 270 589 9 518 1279
Fragments arranged by size:
1279 589 518 270 89 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1384 1826 2316 2754
Size: 1007 377 442 490 438
Fragments arranged by size:
1007 490 442 438 377
BsaI GGTCTCn'nnnn_
Cuts at: 0 1925 2754
Size: 1925 829
BsaBI GATnn'nnATC
Cuts at: 0 1119 1184 2754
Size: 1119 65 1570
Fragments arranged by size:
1570 1119 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1047 1077 1473 1503 2754
Size: 1047 30 396 30 1251
Fragments arranged by size:
1251 1047 396 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2000 2383 2754
Size: 2000 383 371
Fragments arranged by size:
2000 383 371
BseYI C'CCAG_C
Cuts at: 0 2348 2754
Size: 2348 406
BsiHKAI G_wGCw'C
Cuts at: 0 2266 2754
Size: 2266 488
BsmI GAATG_Cn'
Cuts at: 0 609 1294 1882 2754
Size: 609 685 588 872
Fragments arranged by size:
872 685 609 588
Bsp1286I G_dGCh'C
Cuts at: 0 2266 2754
Size: 2266 488
BsrBI CCG'CTC
Cuts at: 0 2373 2754
Size: 2373 381
BsrDI GCAATG_nn'
Cuts at: 0 1057 2754
Size: 1057 1697
BsrGI T'GTAC_A
Cuts at: 0 111 1038 1760 2754
Size: 111 927 722 994
Fragments arranged by size:
994 927 722 111
BssSI C'ACGA_G
Cuts at: 0 1045 2754
Size: 1045 1709
BstAPI GCAn_nnn'nTGC
Cuts at: 0 604 2623 2754
Size: 604 2019 131
Fragments arranged by size:
2019 604 131
BstEII G'GTnAC_C
Cuts at: 0 1997 2754
Size: 1997 757
BstXI CCAn_nnnn'nTGG
Cuts at: 0 995 2754
Size: 995 1759
BstYI r'GATC_y
Cuts at: 0 75 1008 1284 1968 2118 2335 2382 2581
Size: 75 933 276 684 150 217 47 199
Cuts at: 2581 2754
Size: 173
Fragments arranged by size:
933 684 276 217 199 173 150 75
47
Bsu36I CC'TnA_GG
Cuts at: 0 224 2754
Size: 224 2530
BtgI C'CryG_G
Cuts at: 0 2282 2754
Size: 2282 472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1417 2754
Size: 1417 1337
BtsI GCAGTG_nn'
Cuts at: 0 131 2181 2235 2754
Size: 131 2050 54 519
Fragments arranged by size:
2050 519 131 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1030 1065 2754
Size: 1030 35 1689
Fragments arranged by size:
1689 1030 35
DraI TTT'AAA
Cuts at: 0 479 2754
Size: 479 2275
EaeI y'GGCC_r
Cuts at: 0 2374 2754
Size: 2374 380
EarI CTCTTCn'nnn_
Cuts at: 0 1904 2754
Size: 1904 850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 659 2754
Size: 659 2095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 359 842 948 957 1475 2183 2754
Size: 89 270 483 106 9 518 708 571
Fragments arranged by size:
708 571 518 483 270 106 89 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1559 2754
Size: 613 946 1195
Fragments arranged by size:
1195 946 613
EcoO109I rG'GnC_Cy
Cuts at: 0 1564 2754
Size: 1564 1190
EcoRI G'AATT_C
Cuts at: 0 1813 2754
Size: 1813 941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 817 849 961 993 1047 1079
Size: 691 32 94 32 112 32 54 32
Cuts at: 1079 1152 1184 2110 2142 2544 2576 2754
Size: 73 32 926 32 402 32 178
Fragments arranged by size:
926 691 402 178 112 94 73 54
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 5 576 2754
Size: 5 571 2178
Fragments arranged by size:
2178 571 5
KpnI G_GTAC'C
Cuts at: 0 1427 2754
Size: 1427 1327
MslI CAynn'nnrTG
Cuts at: 0 179 1864 2174 2405 2754
Size: 179 1685 310 231 349
Fragments arranged by size:
1685 349 310 231 179
MspA1I CmG'CkG
Cuts at: 0 1234 2754
Size: 1234 1520
NsiI A_TGCA'T
Cuts at: 0 2200 2754
Size: 2200 554
NspI r_CATG'y
Cuts at: 0 1314 2202 2703 2748 2754
Size: 1314 888 501 45 6
Fragments arranged by size:
1314 888 501 45 6
PpuMI rG'GwC_Cy
Cuts at: 0 1564 2754
Size: 1564 1190
PsiI TTA'TAA
Cuts at: 0 757 2754
Size: 757 1997
PstI C_TGCA'G
Cuts at: 0 138 2458 2619 2754
Size: 138 2320 161 135
Fragments arranged by size:
2320 161 138 135
PvuII CAG'CTG
Cuts at: 0 1234 2754
Size: 1234 1520
ScaI AGT'ACT
Cuts at: 0 2440 2754
Size: 2440 314
SfcI C'TryA_G
Cuts at: 0 59 134 1029 2298 2454 2615 2754
Size: 59 75 895 1269 156 161 139
Fragments arranged by size:
1269 895 161 156 139 75 59
SmlI C'TyrA_G
Cuts at: 0 1022 1363 1841 2331 2754
Size: 1022 341 478 490 423
Fragments arranged by size:
1022 490 478 423 341
SpeI A'CTAG_T
Cuts at: 0 1747 2754
Size: 1747 1007
SphI G_CATG'C
Cuts at: 0 2703 2754
Size: 2703 51
SspI AAT'ATT
Cuts at: 0 294 2754
Size: 294 2460
StyI C'CwwG_G
Cuts at: 0 609 1336 2014 2754
Size: 609 727 678 740
Fragments arranged by size:
740 727 678 609
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1308 2754
Size: 1308 1446
TatI w'GTAC_w
Cuts at: 0 111 1038 1760 2438 2754
Size: 111 927 722 678 316
Fragments arranged by size:
927 722 678 316 111
XbaI T'CTAG_A
Cuts at: 0 72 2754
Size: 72 2682
Enzymes that do cut:
AccI Acc65I AcuI AleI AloI AlwNI ApoI AseI
BaeI BanI BbsI BciVI BclI BfrBI BglII BlpI
BmrI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII KpnI MslI MspA1I NsiI NspI PpuMI
PsiI PstI PvuII ScaI SfcI SmlI SpeI SphI
SspI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI
BanII BbeI BbvCI BcgI BglI Bme1580I BmgBI BmtI
BplI Bpu10I BsaAI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BssHII BstBI BstZ17I
ClaI DraIII DrdI EagI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI StuI SwaI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI