Restriction map for
pF1KB5572
(Linear) (Six-base) MAPSORT of: KIBB5572.seq Check: 7948 from: 1 to: 1143
KIBB5572.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 82 1143
Size: 82 1061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 640 1143
Size: 280 360 503
Fragments arranged by size:
503 360 280
AflIII A'CryG_T
Cuts at: 0 364 1143
Size: 364 779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 1143
Size: 401 32 710
Fragments arranged by size:
710 401 32
AlwNI CAG_nnn'CTG
Cuts at: 0 296 1143
Size: 296 847
ApaLI G'TGCA_C
Cuts at: 0 360 1143
Size: 360 783
ApoI r'AATT_y
Cuts at: 0 1132 1143
Size: 1132 11
BanI G'GyrC_C
Cuts at: 0 105 241 376 1143
Size: 105 136 135 767
Fragments arranged by size:
767 136 135 105
BanII G_rGCy'C
Cuts at: 0 7 279 1143
Size: 7 272 864
Fragments arranged by size:
864 272 7
BbeI G_GCGC'C
Cuts at: 0 109 245 380 1143
Size: 109 136 135 763
Fragments arranged by size:
763 136 135 109
BglI GCCn_nnn'nGGC
Cuts at: 0 536 1143
Size: 536 607
Bme1580I G_kGCm'C
Cuts at: 0 364 1143
Size: 364 779
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 1143
Size: 705 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 294 1143
Size: 121 173 849
Fragments arranged by size:
849 173 121
BsaHI Gr'CG_yC
Cuts at: 0 106 242 377 1143
Size: 106 136 135 766
Fragments arranged by size:
766 136 135 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 1143
Size: 401 30 712
Fragments arranged by size:
712 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 1143
Size: 490 653
BseYI C'CCAG_C
Cuts at: 0 546 974 1143
Size: 546 428 169
Fragments arranged by size:
546 428 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1143
Size: 951 192
BsiEI CG_ry'CG
Cuts at: 0 960 1143
Size: 960 183
BsiHKAI G_wGCw'C
Cuts at: 0 7 279 364 718 1143
Size: 7 272 85 354 425
Fragments arranged by size:
425 354 272 85 7
BsmI GAATG_Cn'
Cuts at: 0 235 389 1143
Size: 235 154 754
Fragments arranged by size:
754 235 154
Bsp1286I G_dGCh'C
Cuts at: 0 7 279 364 718 1143
Size: 7 272 85 354 425
Fragments arranged by size:
425 354 272 85 7
BspHI T'CATG_A
Cuts at: 0 1040 1143
Size: 1040 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 1143
Size: 82 1061
BsrBI CCG'CTC
Cuts at: 0 256 1143
Size: 256 887
BsrFI r'CCGG_y
Cuts at: 0 926 1143
Size: 926 217
BsrGI T'GTAC_A
Cuts at: 0 827 1143
Size: 827 316
BssHII G'CGCG_C
Cuts at: 0 116 1143
Size: 116 1027
BssSI C'ACGA_G
Cuts at: 0 746 1143
Size: 746 397
BstAPI GCAn_nnn'nTGC
Cuts at: 0 854 1143
Size: 854 289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1143
Size: 225 918
BstYI r'GATC_y
Cuts at: 0 266 1143
Size: 266 877
BtgI C'CryG_G
Cuts at: 0 380 512 1143
Size: 380 132 631
Fragments arranged by size:
631 380 132
BtsI GCAGTG_nn'
Cuts at: 0 90 472 1143
Size: 90 382 671
Fragments arranged by size:
671 382 90
EaeI y'GGCC_r
Cuts at: 0 957 1143
Size: 957 186
EagI C'GGCC_G
Cuts at: 0 957 1143
Size: 957 186
EcoICRI GAG'CTC
Cuts at: 0 5 277 1143
Size: 5 272 866
Fragments arranged by size:
866 272 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 280 294 640 1143
Size: 121 159 14 346 503
Fragments arranged by size:
503 346 159 121 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 671 841 1107 1143
Size: 538 133 170 266 36
Fragments arranged by size:
538 266 170 133 36
EcoO109I rG'GnC_Cy
Cuts at: 0 507 1143
Size: 507 636
HaeII r_GCGC'y
Cuts at: 0 25 67 109 245 380 1143
Size: 25 42 42 136 135 763
Fragments arranged by size:
763 136 135 42 42 25
HincII GTy'rAC
Cuts at: 0 575 1143
Size: 575 568
KasI G'GCGC_C
Cuts at: 0 105 241 376 1143
Size: 105 136 135 767
Fragments arranged by size:
767 136 135 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1143
Size: 582 561
MslI CAynn'nnrTG
Cuts at: 0 1085 1143
Size: 1085 58
MspA1I CmG'CkG
Cuts at: 0 82 978 1007 1143
Size: 82 896 29 136
Fragments arranged by size:
896 136 82 29
NarI GG'CG_CC
Cuts at: 0 106 242 377 1143
Size: 106 136 135 766
Fragments arranged by size:
766 136 135 106
NcoI C'CATG_G
Cuts at: 0 512 1143
Size: 512 631
NspI r_CATG'y
Cuts at: 0 368 1084 1143
Size: 368 716 59
Fragments arranged by size:
716 368 59
PciI A'CATG_T
Cuts at: 0 364 1143
Size: 364 779
PfoI T'CCnGG_A
Cuts at: 0 137 1143
Size: 137 1006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 1143
Size: 401 32 710
Fragments arranged by size:
710 401 32
PpuMI rG'GwC_Cy
Cuts at: 0 507 1143
Size: 507 636
PstI C_TGCA'G
Cuts at: 0 193 274 1143
Size: 193 81 869
Fragments arranged by size:
869 193 81
PvuII CAG'CTG
Cuts at: 0 978 1143
Size: 978 165
SacI G_AGCT'C
Cuts at: 0 7 279 1143
Size: 7 272 864
Fragments arranged by size:
864 272 7
ScaI AGT'ACT
Cuts at: 0 937 1143
Size: 937 206
SfcI C'TryA_G
Cuts at: 0 189 270 1107 1143
Size: 189 81 837 36
Fragments arranged by size:
837 189 81 36
SfoI GGC'GCC
Cuts at: 0 107 243 378 1143
Size: 107 136 135 765
Fragments arranged by size:
765 136 135 107
SphI G_CATG'C
Cuts at: 0 1084 1143
Size: 1084 59
StyI C'CwwG_G
Cuts at: 0 218 512 534 1143
Size: 218 294 22 609
Fragments arranged by size:
609 294 218 22
TatI w'GTAC_w
Cuts at: 0 827 935 1143
Size: 827 108 208
Fragments arranged by size:
827 208 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1143
Size: 809 32 302
Fragments arranged by size:
809 302 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 1143
Size: 444 699
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaLI ApoI BanI
BanII BbeI BglI Bme1580I BmrI BpmI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI
BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI
EcoO109I HaeII HincII KasI MmeI MslI MspA1I NarI
NcoI NspI PciI PfoI PpiI PpuMI PstI PvuII
SacI ScaI SfcI SfoI SphI StyI TatI TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BsrDI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI