Restriction map for
pF1KB5582
(Linear) (Six-base) MAPSORT of: KIBB5582.seq Check: 180 from: 1 to: 1122
KIBB5582.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
Acc65I G'GTAC_C
Cuts at: 0 376 1122
Size: 376 746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 872 1122
Size: 268 604 250
Fragments arranged by size:
604 268 250
AgeI A'CCGG_T
Cuts at: 0 532 1122
Size: 532 590
AleI CACnn'nnGTG
Cuts at: 0 451 1122
Size: 451 671
ApoI r'AATT_y
Cuts at: 0 301 682 1030 1122
Size: 301 381 348 92
Fragments arranged by size:
381 348 301 92
AseI AT'TA_AT
Cuts at: 0 364 1122
Size: 364 758
BanI G'GyrC_C
Cuts at: 0 81 376 1122
Size: 81 295 746
Fragments arranged by size:
746 295 81
BfrBI ATG'CAT
Cuts at: 0 1086 1122
Size: 1086 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 591 1122
Size: 591 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 856 1122
Size: 335 521 266
Fragments arranged by size:
521 335 266
BsaHI Gr'CG_yC
Cuts at: 0 860 1122
Size: 860 262
BsaWI w'CCGG_w
Cuts at: 0 532 1122
Size: 532 590
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 617 794 1122
Size: 82 535 177 328
Fragments arranged by size:
535 328 177 82
BseYI C'CCAG_C
Cuts at: 0 854 1122
Size: 854 268
BsiHKAI G_wGCw'C
Cuts at: 0 761 1122
Size: 761 361
BsmI GAATG_Cn'
Cuts at: 0 301 1086 1100 1122
Size: 301 785 14 22
Fragments arranged by size:
785 301 22 14
Bsp1286I G_dGCh'C
Cuts at: 0 761 1122
Size: 761 361
BspHI T'CATG_A
Cuts at: 0 96 821 1122
Size: 96 725 301
Fragments arranged by size:
725 301 96
BsrDI GCAATG_nn'
Cuts at: 0 116 1122
Size: 116 1006
BsrFI r'CCGG_y
Cuts at: 0 532 1122
Size: 532 590
BstBI TT'CG_AA
Cuts at: 0 105 1104 1122
Size: 105 999 18
Fragments arranged by size:
999 105 18
BstYI r'GATC_y
Cuts at: 0 480 1122
Size: 480 642
Bsu36I CC'TnA_GG
Cuts at: 0 170 728 1122
Size: 170 558 394
Fragments arranged by size:
558 394 170
BtsI GCAGTG_nn'
Cuts at: 0 122 554 947 1122
Size: 122 432 393 175
Fragments arranged by size:
432 393 175 122
DraIII CAC_nnn'GTG
Cuts at: 0 289 1122
Size: 289 833
EarI CTCTTCn'nnn_
Cuts at: 0 961 1122
Size: 961 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 872 1122
Size: 268 604 250
Fragments arranged by size:
604 268 250
EcoRV GAT'ATC
Cuts at: 0 462 1122
Size: 462 660
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 526 1122
Size: 526 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 1061 1122
Size: 1029 32 61
Fragments arranged by size:
1029 61 32
KpnI G_GTAC'C
Cuts at: 0 380 1122
Size: 380 742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 588 855 1122
Size: 545 43 267 267
Fragments arranged by size:
545 267 267 43
MslI CAynn'nnrTG
Cuts at: 0 140 203 451 661 766 1122
Size: 140 63 248 210 105 356
Fragments arranged by size:
356 248 210 140 105 63
MspA1I CmG'CkG
Cuts at: 0 921 1122
Size: 921 201
NsiI A_TGCA'T
Cuts at: 0 1088 1122
Size: 1088 34
NspI r_CATG'y
Cuts at: 0 845 1122
Size: 845 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 289 1122
Size: 289 833
PfoI T'CCnGG_A
Cuts at: 0 649 749 1122
Size: 649 100 373
Fragments arranged by size:
649 373 100
PstI C_TGCA'G
Cuts at: 0 33 1122
Size: 33 1089
PvuII CAG'CTG
Cuts at: 0 921 1122
Size: 921 201
SfcI C'TryA_G
Cuts at: 0 29 1122
Size: 29 1093
SmlI C'TyrA_G
Cuts at: 0 350 835 1122
Size: 350 485 287
Fragments arranged by size:
485 350 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 787 1122
Size: 787 335
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 263 295 645 677 1122
Size: 263 32 350 32 445
Fragments arranged by size:
445 350 263 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 262 1122
Size: 262 860
Enzymes that do cut:
Acc65I AcuI AgeI AleI ApoI AseI BanI BfrBI
BplI BpuEI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BstBI BstYI Bsu36I BtsI
DraIII EarI Eco57MI EcoRV FalI Hin4I KpnI MmeI
MslI MspA1I NsiI NspI PflMI PfoI PstI PvuII
SfcI SmlI TaqII TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI XbaI XcmI XhoI XmaI XmnI ZraI