Restriction map for
pF1KB5584
(Linear) (Six-base) MAPSORT of: KIBB5584.seq Check: 8534 from: 1 to: 1923
KIBB5584.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
Acc65I G'GTAC_C
Cuts at: 0 1616 1923
Size: 1616 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1923
Size: 1081 842
AflIII A'CryG_T
Cuts at: 0 228 1923
Size: 228 1695
ApoI r'AATT_y
Cuts at: 0 329 395 407 724 774 939 1003 1923
Size: 329 66 12 317 50 165 64 920
Fragments arranged by size:
920 329 317 165 66 64 50 12
BanI G'GyrC_C
Cuts at: 0 1616 1923
Size: 1616 307
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 758 1923
Size: 258 500 1165
Fragments arranged by size:
1165 500 258
BbvCI CC'TCA_GC
Cuts at: 0 1036 1923
Size: 1036 887
BciVI GTATCCnnnnn_n'
Cuts at: 0 32 687 1923
Size: 32 655 1236
Fragments arranged by size:
1236 655 32
BclI T'GATC_A
Cuts at: 0 339 1923
Size: 339 1584
Bme1580I G_kGCm'C
Cuts at: 0 1355 1923
Size: 1355 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 926 975 1851 1923
Size: 926 49 876 72
Fragments arranged by size:
926 876 72 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1736 1923
Size: 1736 187
Bpu10I CC'TnA_GC
Cuts at: 0 1036 1923
Size: 1036 887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1923
Size: 111 1812
BsaI GGTCTCn'nnnn_
Cuts at: 0 503 1923
Size: 503 1420
BsaAI yAC'GTr
Cuts at: 0 502 1474 1923
Size: 502 972 449
Fragments arranged by size:
972 502 449
BsaWI w'CCGG_w
Cuts at: 0 1813 1923
Size: 1813 110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1923
Size: 865 1058
BseYI C'CCAG_C
Cuts at: 0 9 971 1157 1390 1661 1923
Size: 9 962 186 233 271 262
Fragments arranged by size:
962 271 262 233 186 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1562 1923
Size: 1562 361
BsmI GAATG_Cn'
Cuts at: 0 157 992 1923
Size: 157 835 931
Fragments arranged by size:
931 835 157
Bsp1286I G_dGCh'C
Cuts at: 0 1355 1923
Size: 1355 568
BspHI T'CATG_A
Cuts at: 0 342 717 1482 1923
Size: 342 375 765 441
Fragments arranged by size:
765 441 375 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 973 1923
Size: 973 950
BsrDI GCAATG_nn'
Cuts at: 0 938 1923
Size: 938 985
BsrFI r'CCGG_y
Cuts at: 0 238 1923
Size: 238 1685
BsrGI T'GTAC_A
Cuts at: 0 1758 1923
Size: 1758 165
BssSI C'ACGA_G
Cuts at: 0 1313 1923
Size: 1313 610
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1468 1923
Size: 1468 455
BtgI C'CryG_G
Cuts at: 0 276 1923
Size: 276 1647
BtsI GCAGTG_nn'
Cuts at: 0 463 878 1550 1660 1923
Size: 463 415 672 110 263
Fragments arranged by size:
672 463 415 263 110
DraIII CAC_nnn'GTG
Cuts at: 0 146 1845 1923
Size: 146 1699 78
Fragments arranged by size:
1699 146 78
EaeI y'GGCC_r
Cuts at: 0 1676 1923
Size: 1676 247
EarI CTCTTCn'nnn_
Cuts at: 0 183 299 1923
Size: 183 116 1624
Fragments arranged by size:
1624 183 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1736 1923
Size: 1081 655 187
Fragments arranged by size:
1081 655 187
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 1516 1923
Size: 1053 463 407
Fragments arranged by size:
1053 463 407
EcoRI G'AATT_C
Cuts at: 0 724 774 1923
Size: 724 50 1149
Fragments arranged by size:
1149 724 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 775 1923
Size: 775 1148
HaeII r_GCGC'y
Cuts at: 0 1158 1923
Size: 1158 765
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 1923
Size: 30 32 1861
Fragments arranged by size:
1861 32 30
HincII GTy'rAC
Cuts at: 0 801 1923
Size: 801 1122
KpnI G_GTAC'C
Cuts at: 0 1620 1923
Size: 1620 303
MfeI C'AATT_G
Cuts at: 0 877 1923
Size: 877 1046
MscI TGG'CCA
Cuts at: 0 1678 1923
Size: 1678 245
MslI CAynn'nnrTG
Cuts at: 0 233 594 1754 1923
Size: 233 361 1160 169
Fragments arranged by size:
1160 361 233 169
NcoI C'CATG_G
Cuts at: 0 276 1923
Size: 276 1647
NdeI CA'TA_TG
Cuts at: 0 591 1019 1491 1923
Size: 591 428 472 432
Fragments arranged by size:
591 472 432 428
NspI r_CATG'y
Cuts at: 0 232 571 1923
Size: 232 339 1352
Fragments arranged by size:
1352 339 232
PciI A'CATG_T
Cuts at: 0 228 1923
Size: 228 1695
PpuMI rG'GwC_Cy
Cuts at: 0 1053 1516 1923
Size: 1053 463 407
Fragments arranged by size:
1053 463 407
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1792 1824 1923
Size: 1792 32 99
Fragments arranged by size:
1792 99 32
PstI C_TGCA'G
Cuts at: 0 984 1923
Size: 984 939
SfcI C'TryA_G
Cuts at: 0 582 980 1286 1923
Size: 582 398 306 637
Fragments arranged by size:
637 582 398 306
SmlI C'TyrA_G
Cuts at: 0 90 1923
Size: 90 1833
SspI AAT'ATT
Cuts at: 0 1795 1923
Size: 1795 128
StuI AGG'CCT
Cuts at: 0 352 821 1923
Size: 352 469 1102
Fragments arranged by size:
1102 469 352
StyI C'CwwG_G
Cuts at: 0 276 353 925 1923
Size: 276 77 572 998
Fragments arranged by size:
998 572 276 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 584 658 1833 1923
Size: 584 74 1175 90
Fragments arranged by size:
1175 584 90 74
TatI w'GTAC_w
Cuts at: 0 1758 1923
Size: 1758 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1623 1655 1923
Size: 1623 32 268
Fragments arranged by size:
1623 268 32
Enzymes that do cut:
Acc65I AcuI AflIII ApoI BanI BbsI BbvCI BciVI
BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BtgI BtsI DraIII
EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KpnI MfeI MscI MslI NcoI NdeI NspI
PciI PpuMI PsrI PstI SfcI SmlI SspI StuI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BcgI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaBI
BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KasI MluI
MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI