(Linear) (Six-base) MAPSORT of: KIBB5585.seq  Check: 428  from: 1  to: 975



KIBB5585.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AflIII A'CryG_T

Cuts at:      0     821     975 
   Size:        821     154
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171     975 
   Size:        171     804
 
ApoI r'AATT_y

Cuts at:      0      28     390     763     975 
   Size:         28     362     373     212

  Fragments arranged by size: 

                 373     362     212      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     975 
   Size:        299     676
 
BclI T'GATC_A

Cuts at:      0     359     975 
   Size:        359     616
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     975 
   Size:        159     816
 
BmtI G_CTAG'C

Cuts at:      0     566     975 
   Size:        566     409
 
BsaBI GATnn'nnATC

Cuts at:      0     106     975 
   Size:        106     869
 
BsaHI Gr'CG_yC

Cuts at:      0     441     975 
   Size:        441     534
 
BsmI GAATG_Cn'

Cuts at:      0     845     975 
   Size:        845     130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     766     975 
   Size:        766     209
 
BstYI r'GATC_y

Cuts at:      0     270     975 
   Size:        270     705
 
BtgI C'CryG_G

Cuts at:      0     658     975 
   Size:        658     317
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     784     975 
   Size:        784     191
 
BtsI GCAGTG_nn'

Cuts at:      0     137     548     975 
   Size:        137     411     427

  Fragments arranged by size: 

                 427     411     137
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     714     749     975 
   Size:        714      35     226

  Fragments arranged by size: 

                 714     226      35
 
EaeI y'GGCC_r

Cuts at:      0     479     975 
   Size:        479     496
 
EarI CTCTTCn'nnn_

Cuts at:      0     304     936     975 
   Size:        304     632      39

  Fragments arranged by size: 

                 632     304      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     280     857     975 
   Size:        280     577     118

  Fragments arranged by size: 

                 577     280     118
 
EcoRI G'AATT_C

Cuts at:      0      28     975 
   Size:         28     947
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304     598     630     975 
   Size:        272      32     294      32     345

  Fragments arranged by size: 

                 345     294     272      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     975 
   Size:         18     957
 
MscI TGG'CCA

Cuts at:      0     481     975 
   Size:        481     494
 
MslI CAynn'nnrTG

Cuts at:      0     872     975 
   Size:        872     103
 
MspA1I CmG'CkG

Cuts at:      0      14     975 
   Size:         14     961
 
NcoI C'CATG_G

Cuts at:      0     658     975 
   Size:        658     317
 
NheI G'CTAG_C

Cuts at:      0     562     975 
   Size:        562     413
 
NspI r_CATG'y

Cuts at:      0     825     975 
   Size:        825     150
 
PciI A'CATG_T

Cuts at:      0     821     975 
   Size:        821     154
 
PpuMI rG'GwC_Cy

Cuts at:      0     280     857     975 
   Size:        280     577     118

  Fragments arranged by size: 

                 577     280     118
 
PstI C_TGCA'G

Cuts at:      0     762     975 
   Size:        762     213
 
SfcI C'TryA_G

Cuts at:      0     758     975 
   Size:        758     217
 
SpeI A'CTAG_T

Cuts at:      0     261     975 
   Size:        261     714
 
StyI C'CwwG_G

Cuts at:      0      20     658     975 
   Size:         20     638     317

  Fragments arranged by size: 

                 638     317      20
 
TatI w'GTAC_w

Cuts at:      0     258     336     488     773     975 
   Size:        258      78     152     285     202

  Fragments arranged by size: 

                 285     258     202     152      78
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BbsI     BclI     BmrI     BmtI    BsaBI
    BsaHI     BsmI   BstAPI    BstYI     BtgI    BtgZI     BtsI    CspCI
     EaeI     EarI EcoO109I    EcoRI    Hin4I     MmeI     MscI     MslI
   MspA1I     NcoI     NheI     NspI     PciI    PpuMI     PstI     SfcI
     SpeI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI