Restriction map for
pF1KB5588
(Linear) (Six-base) MAPSORT of: KIBB5588.seq Check: 4596 from: 1 to: 2175
KIBB5588.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 606 1493 2175
Size: 606 887 682
Fragments arranged by size:
887 682 606
AccI GT'mk_AC
Cuts at: 0 527 558 2077 2175
Size: 527 31 1519 98
Fragments arranged by size:
1519 527 98 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 756 1780 1835 2168 2175
Size: 670 86 1024 55 333 7
Fragments arranged by size:
1024 670 333 86 55 7
AfeI AGC'GCT
Cuts at: 0 511 2175
Size: 511 1664
AhdI GACnn_n'nnGTC
Cuts at: 0 1644 1830 2175
Size: 1644 186 345
Fragments arranged by size:
1644 345 186
AleI CACnn'nnGTG
Cuts at: 0 1604 2175
Size: 1604 571
ApaI G_GGCC'C
Cuts at: 0 2163 2175
Size: 2163 12
AvaI C'yCGr_G
Cuts at: 0 56 989 1079 1367 2175
Size: 56 933 90 288 808
Fragments arranged by size:
933 808 288 90 56
AvrII C'CTAG_G
Cuts at: 0 897 2175
Size: 897 1278
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1317 1350 2175
Size: 1317 33 825
Fragments arranged by size:
1317 825 33
BanI G'GyrC_C
Cuts at: 0 1889 2175
Size: 1889 286
BanII G_rGCy'C
Cuts at: 0 524 1078 1367 1988 2163 2175
Size: 524 554 289 621 175 12
Fragments arranged by size:
621 554 524 289 175 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1421 1647 2175
Size: 1421 226 528
Fragments arranged by size:
1421 528 226
BbvCI CC'TCA_GC
Cuts at: 0 1876 2175
Size: 1876 299
BciVI GTATCCnnnnn_n'
Cuts at: 0 1318 2175
Size: 1318 857
BclI T'GATC_A
Cuts at: 0 389 1278 2175
Size: 389 889 897
Fragments arranged by size:
897 889 389
BglI GCCn_nnn'nGGC
Cuts at: 0 32 2011 2175
Size: 32 1979 164
Fragments arranged by size:
1979 164 32
BglII A'GATC_T
Cuts at: 0 12 2175
Size: 12 2163
Bme1580I G_kGCm'C
Cuts at: 0 877 2163 2175
Size: 877 1286 12
Fragments arranged by size:
1286 877 12
BmgBI CAC'GTC
Cuts at: 0 552 1161 2175
Size: 552 609 1014
Fragments arranged by size:
1014 609 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 1325 2175
Size: 1325 850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 703 1160 1684 1711 1954 2065 2175
Size: 195 508 457 524 27 243 111 110
Fragments arranged by size:
524 508 457 243 195 111 110 27
Bpu10I CC'TnA_GC
Cuts at: 0 1361 1876 2175
Size: 1361 515 299
Fragments arranged by size:
1361 515 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1068 2175
Size: 1068 1107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1265 2175
Size: 1265 910
BsaAI yAC'GTr
Cuts at: 0 501 2175
Size: 501 1674
BsaBI GATnn'nnATC
Cuts at: 0 637 2175
Size: 637 1538
BsaHI Gr'CG_yC
Cuts at: 0 760 2175
Size: 760 1415
BsaWI w'CCGG_w
Cuts at: 0 440 944 2175
Size: 440 504 1231
Fragments arranged by size:
1231 504 440
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1100 1130 2175
Size: 1100 30 1045
Fragments arranged by size:
1100 1045 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 434 601 1453 1461 1645 2175
Size: 434 167 852 8 184 530
Fragments arranged by size:
852 530 434 184 167 8
BseYI C'CCAG_C
Cuts at: 0 473 2175
Size: 473 1702
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1897 2175
Size: 1897 278
BsiEI CG_ry'CG
Cuts at: 0 417 1502 2175
Size: 417 1085 673
Fragments arranged by size:
1085 673 417
BsiHKAI G_wGCw'C
Cuts at: 0 689 1670 2175
Size: 689 981 505
Fragments arranged by size:
981 689 505
BsmI GAATG_Cn'
Cuts at: 0 1979 2175
Size: 1979 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 862 881 1136 2175
Size: 862 19 255 1039
Fragments arranged by size:
1039 862 255 19
Bsp1286I G_dGCh'C
Cuts at: 0 524 689 877 1078 1367 1670 1988 2163
Size: 524 165 188 201 289 303 318 175
Cuts at: 2163 2175
Size: 12
Fragments arranged by size:
524 318 303 289 201 188 175 165
12
BspEI T'CCGG_A
Cuts at: 0 440 2175
Size: 440 1735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 1493 2175
Size: 606 887 682
Fragments arranged by size:
887 682 606
BsrBI CCG'CTC
Cuts at: 0 201 2175
Size: 201 1974
BsrDI GCAATG_nn'
Cuts at: 0 431 2175
Size: 431 1744
BsrFI r'CCGG_y
Cuts at: 0 1103 2175
Size: 1103 1072
BssSI C'ACGA_G
Cuts at: 0 1600 2175
Size: 1600 575
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 618 832 1606 2175
Size: 501 117 214 774 569
Fragments arranged by size:
774 569 501 214 117
BstYI r'GATC_y
Cuts at: 0 12 837 857 2175
Size: 12 825 20 1318
Fragments arranged by size:
1318 825 20 12
BstZ17I GTA'TAC
Cuts at: 0 2078 2175
Size: 2078 97
BtgI C'CryG_G
Cuts at: 0 906 1502 1586 2175
Size: 906 596 84 589
Fragments arranged by size:
906 596 589 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 755 2113 2175
Size: 755 1358 62
Fragments arranged by size:
1358 755 62
BtsI GCAGTG_nn'
Cuts at: 0 484 1010 1925 2175
Size: 484 526 915 250
Fragments arranged by size:
915 526 484 250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1213 1248 2175
Size: 1213 35 927
Fragments arranged by size:
1213 927 35
EaeI y'GGCC_r
Cuts at: 0 45 63 414 798 1105 1196 1234 1377
Size: 45 18 351 384 307 91 38 143
Cuts at: 1377 1499 1583 1866 2003 2123 2175
Size: 122 84 283 137 120 52
Fragments arranged by size:
384 351 307 283 143 137 122 120
91 84 52 45 38 18
EagI C'GGCC_G
Cuts at: 0 414 1499 2175
Size: 414 1085 676
Fragments arranged by size:
1085 676 414
EarI CTCTTCn'nnn_
Cuts at: 0 679 925 1766 2144 2175
Size: 679 246 841 378 31
Fragments arranged by size:
841 679 378 246 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 13 742 2175
Size: 13 729 1433
Fragments arranged by size:
1433 729 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 670 703 756 1160 1684 1711 1780
Size: 195 475 33 53 404 524 27 69
Cuts at: 1780 1835 1954 2065 2168 2175
Size: 55 119 111 103 7
Fragments arranged by size:
524 475 404 195 119 111 103 69
55 53 33 27 7
EcoO109I rG'GnC_Cy
Cuts at: 0 218 1088 1908 2052 2175
Size: 218 870 820 144 123
Fragments arranged by size:
870 820 218 144 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 1062 2175
Size: 690 372 1113
Fragments arranged by size:
1113 690 372
HaeII r_GCGC'y
Cuts at: 0 513 919 1614 2175
Size: 513 406 695 561
Fragments arranged by size:
695 561 513 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 387 419 1630 1662 1816 1848
Size: 124 32 231 32 1211 32 154 32
Cuts at: 1848 2175
Size: 327
Fragments arranged by size:
1211 327 231 154 124 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 518 746 2175
Size: 482 36 228 1429
Fragments arranged by size:
1429 482 228 36
MscI TGG'CCA
Cuts at: 0 65 1236 1379 1585 1868 2005 2125 2175
Size: 65 1171 143 206 283 137 120 50
Fragments arranged by size:
1171 283 206 143 137 120 65 50
MslI CAynn'nnrTG
Cuts at: 0 71 631 1604 2175
Size: 71 560 973 571
Fragments arranged by size:
973 571 560 71
MspA1I CmG'CkG
Cuts at: 0 215 807 908 1322 1813 2175
Size: 215 592 101 414 491 362
Fragments arranged by size:
592 491 414 362 215 101
NaeI GCC'GGC
Cuts at: 0 1105 2175
Size: 1105 1070
NcoI C'CATG_G
Cuts at: 0 1586 2175
Size: 1586 589
NgoMIV G'CCGG_C
Cuts at: 0 1103 2175
Size: 1103 1072
NspI r_CATG'y
Cuts at: 0 285 472 2175
Size: 285 187 1703
Fragments arranged by size:
1703 285 187
PasI CC'CwG_GG
Cuts at: 0 293 2175
Size: 293 1882
PflMI CCAn_nnn'nTGG
Cuts at: 0 578 2175
Size: 578 1597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2008 2040 2175
Size: 2008 32 135
Fragments arranged by size:
2008 135 32
PpuMI rG'GwC_Cy
Cuts at: 0 218 1088 2052 2175
Size: 218 870 964 123
Fragments arranged by size:
964 870 218 123
PshAI GACnn'nnGTC
Cuts at: 0 554 2175
Size: 554 1621
PspOMI G'GGCC_C
Cuts at: 0 2159 2175
Size: 2159 16
PvuII CAG'CTG
Cuts at: 0 807 2175
Size: 807 1368
SacII CC_GC'GG
Cuts at: 0 909 2175
Size: 909 1266
SanDI GG'GwC_CC
Cuts at: 0 218 2175
Size: 218 1957
SapI GCTCTTCn'nnn_
Cuts at: 0 925 2175
Size: 925 1250
ScaI AGT'ACT
Cuts at: 0 175 2134 2175
Size: 175 1959 41
Fragments arranged by size:
1959 175 41
SfcI C'TryA_G
Cuts at: 0 177 559 2175
Size: 177 382 1616
Fragments arranged by size:
1616 382 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2011 2175
Size: 2011 164
SmaI CCC'GGG
Cuts at: 0 991 2175
Size: 991 1184
SmlI C'TyrA_G
Cuts at: 0 1047 2175
Size: 1047 1128
SphI G_CATG'C
Cuts at: 0 472 2175
Size: 472 1703
StuI AGG'CCT
Cuts at: 0 769 2155 2175
Size: 769 1386 20
Fragments arranged by size:
1386 769 20
StyI C'CwwG_G
Cuts at: 0 161 897 1586 2175
Size: 161 736 689 589
Fragments arranged by size:
736 689 589 161
TatI w'GTAC_w
Cuts at: 0 173 539 704 713 1718 2132 2175
Size: 173 366 165 9 1005 414 43
Fragments arranged by size:
1005 414 366 173 165 43 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1100 1132 2175
Size: 1100 32 1043
Fragments arranged by size:
1100 1043 32
XbaI T'CTAG_A
Cuts at: 0 1284 2175
Size: 1284 891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1704 1842 2175
Size: 1704 138 333
Fragments arranged by size:
1704 333 138
XmaI C'CCGG_G
Cuts at: 0 989 2175
Size: 989 1186
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI ApaI AvaI
AvrII BaeI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PpiI PpuMI
PshAI PspOMI PvuII SacII SanDI SapI ScaI SfcI
SfiI SmaI SmlI SphI StuI StyI TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI BamHI BbeI
BcgI BfrBI BlpI BmtI BplI BsiWI BspHI BsrGI
BssHII BstAPI BstBI BstEII Bsu36I ClaI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SbfI SexAI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XhoI XmnI
ZraI