Restriction map for
pF1KB5596
(Linear) (Six-base) MAPSORT of: KIBB5596.seq Check: 5721 from: 1 to: 1983
KIBB5596.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1175 1983
Size: 1175 808
AclI AA'CG_TT
Cuts at: 0 607 1983
Size: 607 1376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1983
Size: 841 1142
AfeI AGC'GCT
Cuts at: 0 1378 1983
Size: 1378 605
AflIII A'CryG_T
Cuts at: 0 1837 1983
Size: 1837 146
AhdI GACnn_n'nnGTC
Cuts at: 0 1391 1983
Size: 1391 592
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1481 1513 1983
Size: 1481 32 470
Fragments arranged by size:
1481 470 32
ApoI r'AATT_y
Cuts at: 0 484 1084 1523 1983
Size: 484 600 439 460
Fragments arranged by size:
600 484 460 439
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1118 1151 1983
Size: 1118 33 832
Fragments arranged by size:
1118 832 33
BanI G'GyrC_C
Cuts at: 0 944 1983
Size: 944 1039
BanII G_rGCy'C
Cuts at: 0 176 419 1983
Size: 176 243 1564
Fragments arranged by size:
1564 243 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 794 1343 1754 1983
Size: 794 549 411 229
Fragments arranged by size:
794 549 411 229
BbvCI CC'TCA_GC
Cuts at: 0 264 1983
Size: 264 1719
BciVI GTATCCnnnnn_n'
Cuts at: 0 1152 1983
Size: 1152 831
BglI GCCn_nnn'nGGC
Cuts at: 0 13 1983
Size: 13 1970
Bme1580I G_kGCm'C
Cuts at: 0 1835 1983
Size: 1835 148
BmrI ACTGGGnnnn_n'
Cuts at: 0 337 357 522 1983
Size: 337 20 165 1461
Fragments arranged by size:
1461 337 165 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1346 1983
Size: 1346 637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 709 1409 1983
Size: 691 18 700 574
Fragments arranged by size:
700 691 574 18
Bpu10I CC'TnA_GC
Cuts at: 0 264 411 1983
Size: 264 147 1572
Fragments arranged by size:
1572 264 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1500 1983
Size: 325 1175 483
Fragments arranged by size:
1175 483 325
BsaAI yAC'GTr
Cuts at: 0 796 1840 1983
Size: 796 1044 143
Fragments arranged by size:
1044 796 143
BsaBI GATnn'nnATC
Cuts at: 0 974 1430 1983
Size: 974 456 553
Fragments arranged by size:
974 553 456
BsaHI Gr'CG_yC
Cuts at: 0 721 1983
Size: 721 1262
BsaWI w'CCGG_w
Cuts at: 0 1155 1983
Size: 1155 828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 893 923 1983
Size: 893 30 1060
Fragments arranged by size:
1060 893 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 38 41 44 68 71 294 427
Size: 35 3 3 3 24 3 223 133
Cuts at: 427 554 1983
Size: 127 1429
Fragments arranged by size:
1429 223 133 127 35 24 3 3
3 3
BsiEI CG_ry'CG
Cuts at: 0 254 1983
Size: 254 1729
BsmI GAATG_Cn'
Cuts at: 0 558 746 1969 1983
Size: 558 188 1223 14
Fragments arranged by size:
1223 558 188 14
Bsp1286I G_dGCh'C
Cuts at: 0 176 419 1835 1983
Size: 176 243 1416 148
Fragments arranged by size:
1416 243 176 148
BspEI T'CCGG_A
Cuts at: 0 1155 1983
Size: 1155 828
BspHI T'CATG_A
Cuts at: 0 1332 1983
Size: 1332 651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1175 1983
Size: 1175 808
BsrDI GCAATG_nn'
Cuts at: 0 1471 1983
Size: 1471 512
BsrGI T'GTAC_A
Cuts at: 0 1293 1983
Size: 1293 690
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 1983
Size: 169 1814
BtgI C'CryG_G
Cuts at: 0 146 892 1983
Size: 146 746 1091
Fragments arranged by size:
1091 746 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1740 1983
Size: 1740 243
BtsI GCAGTG_nn'
Cuts at: 0 1773 1983
Size: 1773 210
EaeI y'GGCC_r
Cuts at: 0 251 314 1611 1886 1983
Size: 251 63 1297 275 97
Fragments arranged by size:
1297 275 251 97 63
EagI C'GGCC_G
Cuts at: 0 251 1983
Size: 251 1732
EarI CTCTTCn'nnn_
Cuts at: 0 312 1952 1983
Size: 312 1640 31
Fragments arranged by size:
1640 312 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 183 276 1983
Size: 174 9 93 1707
Fragments arranged by size:
1707 174 93 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 709 841 1409 1983
Size: 691 18 132 568 574
Fragments arranged by size:
691 574 568 132 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 916 1983
Size: 916 1067
EcoO109I rG'GnC_Cy
Cuts at: 0 153 684 1545 1983
Size: 153 531 861 438
Fragments arranged by size:
861 531 438 153
EcoRI G'AATT_C
Cuts at: 0 1523 1983
Size: 1523 460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1373 1983
Size: 1373 610
HaeII r_GCGC'y
Cuts at: 0 1380 1895 1983
Size: 1380 515 88
Fragments arranged by size:
1380 515 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 417 449 973 1005 1255 1287 1377 1409
Size: 417 32 524 32 250 32 90 32
Cuts at: 1409 1983
Size: 574
Fragments arranged by size:
574 524 417 250 90 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 1814 1983
Size: 1814 169
MscI TGG'CCA
Cuts at: 0 316 1888 1983
Size: 316 1572 95
Fragments arranged by size:
1572 316 95
MslI CAynn'nnrTG
Cuts at: 0 167 404 1064 1202 1983
Size: 167 237 660 138 781
Fragments arranged by size:
781 660 237 167 138
MspA1I CmG'CkG
Cuts at: 0 148 280 726 1983
Size: 148 132 446 1257
Fragments arranged by size:
1257 446 148 132
NdeI CA'TA_TG
Cuts at: 0 1141 1983
Size: 1141 842
NotI GC'GGCC_GC
Cuts at: 0 251 1983
Size: 251 1732
NspI r_CATG'y
Cuts at: 0 1201 1983
Size: 1201 782
PfoI T'CCnGG_A
Cuts at: 0 686 1983
Size: 686 1297
PpuMI rG'GwC_Cy
Cuts at: 0 153 684 1545 1983
Size: 153 531 861 438
Fragments arranged by size:
861 531 438 153
PstI C_TGCA'G
Cuts at: 0 650 1983
Size: 650 1333
PvuII CAG'CTG
Cuts at: 0 280 1983
Size: 280 1703
SacII CC_GC'GG
Cuts at: 0 149 1983
Size: 149 1834
SfcI C'TryA_G
Cuts at: 0 646 1983
Size: 646 1337
SmlI C'TyrA_G
Cuts at: 0 340 1479 1983
Size: 340 1139 504
Fragments arranged by size:
1139 504 340
SspI AAT'ATT
Cuts at: 0 1953 1983
Size: 1953 30
StyI C'CwwG_G
Cuts at: 0 311 1983
Size: 311 1672
TatI w'GTAC_w
Cuts at: 0 1293 1566 1983
Size: 1293 273 417
Fragments arranged by size:
1293 417 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 1983
Size: 608 32 1343
Fragments arranged by size:
1343 608 32
XbaI T'CTAG_A
Cuts at: 0 978 1983
Size: 978 1005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 1983
Size: 324 1659
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AhdI AloI ApoI
BaeI BanI BanII BbsI BbvCI BciVI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrGI BstXI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I MfeI MscI MslI MspA1I NdeI NotI
NspI PfoI PpuMI PstI PvuII SacII SfcI SmlI
SspI StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AleI AlfI AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BcgI BclI BfrBI BglII BlpI BmgBI BmtI
BsaI BseYI BsgI BsiHKAI BsiWI BsmBI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XhoI XmaI XmnI ZraI