Restriction map for
pF1KB5597
(Linear) (Six-base) MAPSORT of: KIBB5597.seq Check: 984 from: 1 to: 1386
KIBB5597.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 969 1386
Size: 969 417
AccI GT'mk_AC
Cuts at: 0 1224 1386
Size: 1224 162
Acc65I G'GTAC_C
Cuts at: 0 1064 1386
Size: 1064 322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1207 1386
Size: 361 846 179
Fragments arranged by size:
846 361 179
AleI CACnn'nnGTG
Cuts at: 0 310 1386
Size: 310 1076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 328 1386
Size: 328 1058
AlwNI CAG_nnn'CTG
Cuts at: 0 21 52 337 648 699 813 966 1035
Size: 21 31 285 311 51 114 153 69
Cuts at: 1035 1386
Size: 351
Fragments arranged by size:
351 311 285 153 114 69 51 31
21
ApoI r'AATT_y
Cuts at: 0 481 1386
Size: 481 905
AvaI C'yCGr_G
Cuts at: 0 212 1386
Size: 212 1174
BanI G'GyrC_C
Cuts at: 0 118 929 1064 1274 1386
Size: 118 811 135 210 112
Fragments arranged by size:
811 210 135 118 112
BanII G_rGCy'C
Cuts at: 0 1012 1386
Size: 1012 374
BbeI G_GCGC'C
Cuts at: 0 933 1278 1386
Size: 933 345 108
Fragments arranged by size:
933 345 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 1314 1386
Size: 1314 72
BbvCI CC'TCA_GC
Cuts at: 0 635 1279 1361 1386
Size: 635 644 82 25
Fragments arranged by size:
644 635 82 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 732 766 1386
Size: 732 34 620
Fragments arranged by size:
732 620 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 300 1386
Size: 300 1086
BglI GCCn_nnn'nGGC
Cuts at: 0 568 754 1386
Size: 568 186 632
Fragments arranged by size:
632 568 186
BlpI GC'TnA_GC
Cuts at: 0 649 1386
Size: 649 737
Bme1580I G_kGCm'C
Cuts at: 0 121 1386
Size: 121 1265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 255 811 1159 1386
Size: 129 126 556 348 227
Fragments arranged by size:
556 348 227 129 126
Bpu10I CC'TnA_GC
Cuts at: 0 635 1279 1361 1386
Size: 635 644 82 25
Fragments arranged by size:
644 635 82 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1386
Size: 862 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 1386
Size: 679 707
BsaHI Gr'CG_yC
Cuts at: 0 930 1275 1386
Size: 930 345 111
Fragments arranged by size:
930 345 111
BsaWI w'CCGG_w
Cuts at: 0 1014 1386
Size: 1014 372
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 508 538 1386
Size: 445 30 33 30 848
Fragments arranged by size:
848 445 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 93 132 258 652 1386
Size: 88 5 39 126 394 734
Fragments arranged by size:
734 394 126 88 39 5
BseYI C'CCAG_C
Cuts at: 0 301 319 324 330 1386
Size: 301 18 5 6 1056
Fragments arranged by size:
1056 301 18 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 688 826 1386
Size: 568 120 138 560
Fragments arranged by size:
568 560 138 120
BsiEI CG_ry'CG
Cuts at: 0 1252 1386
Size: 1252 134
BsiHKAI G_wGCw'C
Cuts at: 0 1012 1259 1386
Size: 1012 247 127
Fragments arranged by size:
1012 247 127
Bsp1286I G_dGCh'C
Cuts at: 0 121 1012 1259 1386
Size: 121 891 247 127
Fragments arranged by size:
891 247 127 121
BspEI T'CCGG_A
Cuts at: 0 1014 1386
Size: 1014 372
BspHI T'CATG_A
Cuts at: 0 770 1386
Size: 770 616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 707 794 969 1386
Size: 707 87 175 417
Fragments arranged by size:
707 417 175 87
BsrBI CCG'CTC
Cuts at: 0 62 1386
Size: 62 1324
BsrDI GCAATG_nn'
Cuts at: 0 1208 1343 1386
Size: 1208 135 43
Fragments arranged by size:
1208 135 43
BsrFI r'CCGG_y
Cuts at: 0 926 1251 1386
Size: 926 325 135
Fragments arranged by size:
926 325 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 813 1386
Size: 813 573
BstEII G'GTnAC_C
Cuts at: 0 381 1386
Size: 381 1005
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 393 1386
Size: 234 159 993
Fragments arranged by size:
993 234 159
BstYI r'GATC_y
Cuts at: 0 390 1386
Size: 390 996
BtsI GCAGTG_nn'
Cuts at: 0 50 1046 1386
Size: 50 996 340
Fragments arranged by size:
996 340 50
ClaI AT'CG_AT
Cuts at: 0 1241 1386
Size: 1241 145
EaeI y'GGCC_r
Cuts at: 0 1249 1386
Size: 1249 137
EagI C'GGCC_G
Cuts at: 0 1249 1386
Size: 1249 137
EarI CTCTTCn'nnn_
Cuts at: 0 355 1386
Size: 355 1031
EcoICRI GAG'CTC
Cuts at: 0 1010 1386
Size: 1010 376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 255 361 811 1159 1207 1386
Size: 129 126 106 450 348 48 179
Fragments arranged by size:
450 348 179 129 126 106 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 505 790 1386
Size: 505 285 596
Fragments arranged by size:
596 505 285
EcoO109I rG'GnC_Cy
Cuts at: 0 618 696 747 855 897 1338 1386
Size: 618 78 51 108 42 441 48
Fragments arranged by size:
618 441 108 78 51 48 42
EcoRV GAT'ATC
Cuts at: 0 1160 1386
Size: 1160 226
HaeII r_GCGC'y
Cuts at: 0 249 933 1278 1386
Size: 249 684 345 108
Fragments arranged by size:
684 345 249 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 375 407 445 477 881 913
Size: 61 32 282 32 38 32 404 32
Cuts at: 913 1217 1249 1386
Size: 304 32 137
Fragments arranged by size:
404 304 282 137 61 38 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1225 1386
Size: 1225 161
KasI G'GCGC_C
Cuts at: 0 929 1274 1386
Size: 929 345 112
Fragments arranged by size:
929 345 112
KpnI G_GTAC'C
Cuts at: 0 1068 1386
Size: 1068 318
MslI CAynn'nnrTG
Cuts at: 0 310 716 1003 1386
Size: 310 406 287 383
Fragments arranged by size:
406 383 310 287
MspA1I CmG'CkG
Cuts at: 0 401 488 582 645 813 954 1386
Size: 401 87 94 63 168 141 432
Fragments arranged by size:
432 401 168 141 94 87 63
NarI GG'CG_CC
Cuts at: 0 930 1275 1386
Size: 930 345 111
Fragments arranged by size:
930 345 111
NspI r_CATG'y
Cuts at: 0 888 1386
Size: 888 498
PasI CC'CwG_GG
Cuts at: 0 1092 1386
Size: 1092 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 337 1035 1386
Size: 114 223 698 351
Fragments arranged by size:
698 351 223 114
PfoI T'CCnGG_A
Cuts at: 0 512 692 899 1162 1386
Size: 512 180 207 263 224
Fragments arranged by size:
512 263 224 207 180
PpuMI rG'GwC_Cy
Cuts at: 0 696 855 897 1338 1386
Size: 696 159 42 441 48
Fragments arranged by size:
696 441 159 48 42
PshAI GACnn'nnGTC
Cuts at: 0 463 1386
Size: 463 923
PstI C_TGCA'G
Cuts at: 0 414 755 1386
Size: 414 341 631
Fragments arranged by size:
631 414 341
PvuII CAG'CTG
Cuts at: 0 488 582 645 954 1386
Size: 488 94 63 309 432
Fragments arranged by size:
488 432 309 94 63
SacI G_AGCT'C
Cuts at: 0 1012 1386
Size: 1012 374
SalI G'TCGA_C
Cuts at: 0 1223 1386
Size: 1223 163
SbfI CC_TGCA'GG
Cuts at: 0 755 1386
Size: 755 631
SfcI C'TryA_G
Cuts at: 0 410 751 1386
Size: 410 341 635
Fragments arranged by size:
635 410 341
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 568 754 1386
Size: 568 186 632
Fragments arranged by size:
632 568 186
SfoI GGC'GCC
Cuts at: 0 931 1276 1386
Size: 931 345 110
Fragments arranged by size:
931 345 110
SmaI CCC'GGG
Cuts at: 0 214 1386
Size: 214 1172
SmlI C'TyrA_G
Cuts at: 0 877 1386
Size: 877 509
StuI AGG'CCT
Cuts at: 0 112 1386
Size: 112 1274
StyI C'CwwG_G
Cuts at: 0 113 386 680 1106 1386
Size: 113 273 294 426 280
Fragments arranged by size:
426 294 280 273 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 602 1386
Size: 333 269 784
Fragments arranged by size:
784 333 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1238 1270 1386
Size: 1158 32 48 32 116
Fragments arranged by size:
1158 116 48 32 32
XmaI C'CCGG_G
Cuts at: 0 212 1386
Size: 212 1174
XmnI GAAnn'nnTTC
Cuts at: 0 166 1386
Size: 166 1220
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI BtsI ClaI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI
KpnI MslI MspA1I NarI NspI PasI PflMI PfoI
PpuMI PshAI PstI PvuII SacI SalI SbfI SfcI
SfiI SfoI SmaI SmlI StuI StyI TaqII TstI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaAI
BsaBI BsiWI BsmI BsmBI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI
EciI EcoRI FalI FseI FspI FspAI HindIII HpaI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI ZraI