Restriction map for
pF1KB5605
(Linear) (Six-base) MAPSORT of: KIBB5605.seq Check: 3817 from: 1 to: 1725
KIBB5605.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 350 529 1622 1725
Size: 350 179 1093 103
Fragments arranged by size:
1093 350 179 103
Acc65I G'GTAC_C
Cuts at: 0 1378 1725
Size: 1378 347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1092 1304 1725
Size: 1092 212 421
Fragments arranged by size:
1092 421 212
AfeI AGC'GCT
Cuts at: 0 1015 1725
Size: 1015 710
AflIII A'CryG_T
Cuts at: 0 761 1725
Size: 761 964
AgeI A'CCGG_T
Cuts at: 0 962 1725
Size: 962 763
AhdI GACnn_n'nnGTC
Cuts at: 0 1235 1725
Size: 1235 490
AlwNI CAG_nnn'CTG
Cuts at: 0 618 1140 1725
Size: 618 522 585
Fragments arranged by size:
618 585 522
ApaI G_GGCC'C
Cuts at: 0 124 311 1403 1725
Size: 124 187 1092 322
Fragments arranged by size:
1092 322 187 124
ApaLI G'TGCA_C
Cuts at: 0 669 1725
Size: 669 1056
AvaI C'yCGr_G
Cuts at: 0 117 304 1725
Size: 117 187 1421
Fragments arranged by size:
1421 187 117
BanI G'GyrC_C
Cuts at: 0 251 547 580 714 808 1208 1378 1429
Size: 251 296 33 134 94 400 170 51
Cuts at: 1429 1630 1725
Size: 201 95
Fragments arranged by size:
400 296 251 201 170 134 95 94
51 33
BanII G_rGCy'C
Cuts at: 0 124 311 390 545 786 913 998 1094
Size: 124 187 79 155 241 127 85 96
Cuts at: 1094 1403 1484 1535 1725
Size: 309 81 51 190
Fragments arranged by size:
309 241 190 187 155 127 124 96
85 81 79 51
BbeI G_GCGC'C
Cuts at: 0 255 551 718 812 1634 1725
Size: 255 296 167 94 822 91
Fragments arranged by size:
822 296 255 167 94 91
BbvCI CC'TCA_GC
Cuts at: 0 428 1725
Size: 428 1297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 570 604 1305 1339 1548 1582 1725
Size: 570 34 701 34 209 34 143
Fragments arranged by size:
701 570 209 143 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 643 712 806 820 1286 1427 1639 1725
Size: 643 69 94 14 466 141 212 86
Fragments arranged by size:
643 466 212 141 94 86 69 14
BglII A'GATC_T
Cuts at: 0 149 1725
Size: 149 1576
Bme1580I G_kGCm'C
Cuts at: 0 124 311 369 443 673 701 802 1282
Size: 124 187 58 74 230 28 101 480
Cuts at: 1282 1403 1725
Size: 121 322
Fragments arranged by size:
480 322 230 187 124 121 101 74
58 28
BmgBI CAC'GTC
Cuts at: 0 975 1725
Size: 975 750
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 992 1725
Size: 711 281 733
Fragments arranged by size:
733 711 281
BmtI G_CTAG'C
Cuts at: 0 1271 1725
Size: 1271 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1725
Size: 1012 713
Bpu10I CC'TnA_GC
Cuts at: 0 428 1725
Size: 428 1297
BsaI GGTCTCn'nnnn_
Cuts at: 0 925 1725
Size: 925 800
BsaAI yAC'GTr
Cuts at: 0 1695 1725
Size: 1695 30
BsaHI Gr'CG_yC
Cuts at: 0 252 548 715 809 1498 1631 1711 1725
Size: 252 296 167 94 689 133 80 14
Fragments arranged by size:
689 296 252 167 133 94 80 14
BsaWI w'CCGG_w
Cuts at: 0 962 1725
Size: 962 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 817 847 1426 1456 1500 1530
Size: 454 30 333 30 579 30 44 30
Cuts at: 1530 1642 1672 1725
Size: 112 30 53
Fragments arranged by size:
579 454 333 112 53 44 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 180 475 1508 1594 1725
Size: 180 295 1033 86 131
Fragments arranged by size:
1033 295 180 131 86
BseYI C'CCAG_C
Cuts at: 0 442 513 801 1489 1725
Size: 442 71 288 688 236
Fragments arranged by size:
688 442 288 236 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1299 1725
Size: 822 477 426
Fragments arranged by size:
822 477 426
BsiEI CG_ry'CG
Cuts at: 0 953 1526 1725
Size: 953 573 199
Fragments arranged by size:
953 573 199
BsiHKAI G_wGCw'C
Cuts at: 0 98 673 761 881 1725
Size: 98 575 88 120 844
Fragments arranged by size:
844 575 120 98 88
BsmI GAATG_Cn'
Cuts at: 0 1539 1725
Size: 1539 186
BsmBI CGTCTCn'nnnn_
Cuts at: 0 570 1725
Size: 570 1155
Bsp1286I G_dGCh'C
Cuts at: 0 98 124 311 369 390 443 545 673
Size: 98 26 187 58 21 53 102 128
Cuts at: 673 701 761 786 802 881 913 998 1094
Size: 28 60 25 16 79 32 85 96
Cuts at: 1094 1282 1403 1484 1535 1725
Size: 188 121 81 51 190
Fragments arranged by size:
190 188 187 128 121 102 98 96
85 81 79 60 58 53 51 32
28 26 25 21 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 529 1622 1725
Size: 350 179 1093 103
Fragments arranged by size:
1093 350 179 103
BsrBI CCG'CTC
Cuts at: 0 604 688 1707 1725
Size: 604 84 1019 18
Fragments arranged by size:
1019 604 84 18
BsrDI GCAATG_nn'
Cuts at: 0 774 1725
Size: 774 951
BsrFI r'CCGG_y
Cuts at: 0 37 248 621 805 934 941 962 1725
Size: 37 211 373 184 129 7 21 763
Fragments arranged by size:
763 373 211 184 129 37 21 7
BssHII G'CGCG_C
Cuts at: 0 23 1725
Size: 23 1702
BssSI C'ACGA_G
Cuts at: 0 918 1195 1534 1725
Size: 918 277 339 191
Fragments arranged by size:
918 339 277 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 1695 1725
Size: 86 1609 30
Fragments arranged by size:
1609 86 30
BstYI r'GATC_y
Cuts at: 0 149 1725
Size: 149 1576
BtgI C'CryG_G
Cuts at: 0 551 597 638 1094 1526 1725
Size: 551 46 41 456 432 199
Fragments arranged by size:
551 456 432 199 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1551 1725
Size: 1551 174
BtsI GCAGTG_nn'
Cuts at: 0 475 1047 1725
Size: 475 572 678
Fragments arranged by size:
678 572 475
DraIII CAC_nnn'GTG
Cuts at: 0 1033 1725
Size: 1033 692
EaeI y'GGCC_r
Cuts at: 0 35 243 950 1523 1725
Size: 35 208 707 573 202
Fragments arranged by size:
707 573 208 202 35
EagI C'GGCC_G
Cuts at: 0 950 1523 1725
Size: 950 573 202
Fragments arranged by size:
950 573 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 624 1725
Size: 624 1101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1092 1304 1725
Size: 1012 80 212 421
Fragments arranged by size:
1012 421 212 80
EcoO109I rG'GnC_Cy
Cuts at: 0 9 121 308 1725
Size: 9 112 187 1417
Fragments arranged by size:
1417 187 112 9
FseI GG_CCGG'CC
Cuts at: 0 41 1725
Size: 41 1684
FspI TGC'GCA
Cuts at: 0 1618 1725
Size: 1618 107
HaeII r_GCGC'y
Cuts at: 0 255 303 551 619 718 725 812 834
Size: 255 48 248 68 99 7 87 22
Cuts at: 834 1017 1594 1603 1634 1725
Size: 183 577 9 31 91
Fragments arranged by size:
577 255 248 183 99 91 87 68
48 31 22 9 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1221 1253 1426 1456 1458 1488
Size: 817 32 372 32 173 30 2 30
Cuts at: 1488 1725
Size: 237
Fragments arranged by size:
817 372 237 173 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 1023 1725
Size: 1023 702
KasI G'GCGC_C
Cuts at: 0 251 547 714 808 1630 1725
Size: 251 296 167 94 822 95
Fragments arranged by size:
822 296 251 167 95 94
KpnI G_GTAC'C
Cuts at: 0 1382 1725
Size: 1382 343
MluI A'CGCG_T
Cuts at: 0 761 1725
Size: 761 964
MspA1I CmG'CkG
Cuts at: 0 273 424 553 599 640 791 1469 1725
Size: 273 151 129 46 41 151 678 256
Fragments arranged by size:
678 273 256 151 151 129 46 41
NaeI GCC'GGC
Cuts at: 0 39 250 807 1725
Size: 39 211 557 918
Fragments arranged by size:
918 557 211 39
NarI GG'CG_CC
Cuts at: 0 252 548 715 809 1631 1725
Size: 252 296 167 94 822 94
Fragments arranged by size:
822 296 252 167 94 94
NgoMIV G'CCGG_C
Cuts at: 0 37 248 805 1725
Size: 37 211 557 920
Fragments arranged by size:
920 557 211 37
NheI G'CTAG_C
Cuts at: 0 1267 1725
Size: 1267 458
NruI TCG'CGA
Cuts at: 0 1570 1725
Size: 1570 155
NspI r_CATG'y
Cuts at: 0 927 1725
Size: 927 798
PasI CC'CwG_GG
Cuts at: 0 778 1725
Size: 778 947
PfoI T'CCnGG_A
Cuts at: 0 1295 1725
Size: 1295 430
PmlI CAC'GTG
Cuts at: 0 1695 1725
Size: 1695 30
PpuMI rG'GwC_Cy
Cuts at: 0 9 1725
Size: 9 1716
PspOMI G'GGCC_C
Cuts at: 0 120 307 1399 1725
Size: 120 187 1092 326
Fragments arranged by size:
1092 326 187 120
PstI C_TGCA'G
Cuts at: 0 272 526 736 821 1691 1725
Size: 272 254 210 85 870 34
Fragments arranged by size:
870 272 254 210 85 34
PvuII CAG'CTG
Cuts at: 0 273 1725
Size: 273 1452
RsrII CG'GwC_CG
Cuts at: 0 1700 1725
Size: 1700 25
SacII CC_GC'GG
Cuts at: 0 554 600 641 1725
Size: 554 46 41 1084
Fragments arranged by size:
1084 554 46 41
SbfI CC_TGCA'GG
Cuts at: 0 526 821 1725
Size: 526 295 904
Fragments arranged by size:
904 526 295
SexAI A'CCwGG_T
Cuts at: 0 1067 1725
Size: 1067 658
SfcI C'TryA_G
Cuts at: 0 268 354 522 732 817 1687 1725
Size: 268 86 168 210 85 870 38
Fragments arranged by size:
870 268 210 168 86 85 38
SfoI GGC'GCC
Cuts at: 0 253 549 716 810 1632 1725
Size: 253 296 167 94 822 93
Fragments arranged by size:
822 296 253 167 94 93
SgrAI Cr'CCGG_yG
Cuts at: 0 248 962 1725
Size: 248 714 763
Fragments arranged by size:
763 714 248
SmaI CCC'GGG
Cuts at: 0 119 1725
Size: 119 1606
StyI C'CwwG_G
Cuts at: 0 18 1673 1725
Size: 18 1655 52
Fragments arranged by size:
1655 52 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1276 1717 1725
Size: 1276 441 8
Fragments arranged by size:
1276 441 8
TatI w'GTAC_w
Cuts at: 0 1652 1725
Size: 1652 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 1066 1098 1500 1532 1642 1674
Size: 454 32 580 32 402 32 110 32
Cuts at: 1674 1725
Size: 51
Fragments arranged by size:
580 454 402 110 51 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 855 1725
Size: 855 870
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1644 1725
Size: 1644 81
XmaI C'CCGG_G
Cuts at: 0 117 1725
Size: 117 1608
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AhdI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI
BglI BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstYI BtgI BtgZI BtsI
DraIII EaeI EagI EciI Eco57MI EcoO109I FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MspA1I NaeI
NarI NgoMIV NheI NruI NspI PasI PfoI PmlI
PpuMI PspOMI PstI PvuII RsrII SacII SbfI SexAI
SfcI SfoI SgrAI SmaI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AleI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BlpI BplI BpuEI BsaBI BsiWI BspEI
BspHI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FspAI HindIII HpaI MfeI MmeI MscI MslI
NcoI NdeI NotI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI SacI SalI
SanDI SapI ScaI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmnI
ZraI